Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-CDF-EG-PRE-RfA

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16922_A9Aop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-_C_F Sequence file: RDB16922_A9Aoa.seq
>07722_16922_A9Ao_7_EF1a-_C_F_A06_01_ABI08.ab1     1 TTTCTTGGGG AATTTGCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG    61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT   121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA   361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG   601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA   661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT   781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG   841 ACTCAGATCC TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT   901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT   961 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC  1021 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGC  1081 AACGTGCGTG GTGTGCACTG TGTTGCTGGA CGCAAACCCC CCACTGGGTT GGGCATTGCA 1141 ACCACTGGTC AGCTCCTTTT CCGAACTTCG CATTTTCCCC TCCCTATTGG CCCACCGAC //
Primer: Cm-M_R Sequence file: RDB16922_A9Aob.seq
>07722_16922_A9Ao_7_Cm-M_R_B06_02_ABI08.ab1     1 GGCTTGGGGT GAACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAATTC    61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT   121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT   181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC   241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT   301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC   361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA   421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT   481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT   541 TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT   601 GGTCTTAAAG GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT   661 AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT   721 TCTAGGTCTC GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA   781 GGGCGAAGGC GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG   841 TCCGCTGGAT TGAGGGCCGA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AAATCCAGGT   901 GGCAACCACA GGCGAGCAGC CATGGGAAAG GACGTCAGCT TCCCTCGACA ACACCACGGG   961 AATTGTCAGT GGCCCAACAG CCGAGCCCCC TGTCCAGCAG CGGGGCAGGC AAGCGGCGAT  1021 GAGTTCGCCG TGGCATAGGG GAGGGGGGAA AGCGAAAGTC CCGGAAGGGA GCTGACAGGT  1081 GGTGGCAATG CTCCAACCAG TGGGGGTGCC GTCACCAAAC CAGTGCAACC ACGGCACGTT  1141 GGCCTGACAA CGTGCACAAC CTCCTCATAA AGAGACAAGC CACCAGGAAT TTATCCAGGG 1201 AGGGACAAAG TGAAGAGTGC GTGCTGGGTA GGTCAT //
Primer: BGH_rev Sequence file: RDB16922_A9Aoc.seq
>07722_16922_A9Ao_7_BGH_rev_C06_03_ABI08.ab1     1 CCTTAAAAGG GGGGCCTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC    61 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT   121 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG   181 TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC GGGCCATGTG ATGAAATGCT   241 AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG GGTCATCCAT CCCATGCAGG   301 CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG CTTCTTCTAC CTTCTCTTGC   361 TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG GATATCACCA CTTTGTACAA   421 GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA   481 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGTCGACCTG   541 CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC AGGTTAATGG   601 CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG ATAACGGAGA   661 CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG ATATGCACCA   721 CCGGGTAAAG TTCACGGGAG ACTTTATCTG ACAGCAGACG TGCACTGGGC CAGGGGGATC   781 ACCATCCGTC GCCCGGGCGT GTCAATAAAT ATCACTCTGT ACATCCACAA ACAGACGATA   841 ACGGGCTCTC TCTTTTATAG GTGTAAACCT TTAAACTGCA TTTCACCAGC CCCTGTTCTC   901 GTCAGCAAAG AGCCGTTCAT TTCAATAAAA CCGGGCGACC TCAGCCCATC CCTTCCTGAT   961 TTCCGCTTTC CAGCGTTCGG CACGCAGACG ACGGGCTTTC ATTCTGCCAT GGTTGTGCTT  1021 ACCCAGACCG GAGAATATTG ACATTCATAT ATTGCCCTTG AGCACTGGAT AGCCTGTCGC  1081 TGTCCAACTG TACACTGTAA TACCGCTTGC TTCAATAGCA ATACCCTCCT TTTTGACCAT  1141 ACTTCGGGGT ATACATAATC CAGGTATTAT AATTCTTATT AGCGCGCCAA AAAATTCGAG  1201 CGCGGCGCCA ATTACGCGCA TAACTGGTTT ATCTCTGGGG GCCTTTTGTA GAAGTTAAAC 1261 GCAGCCAGCA G //
Primer: CMV-Forward Sequence file: RDB16922_A9Aod.seq
>07722_16922_A9Ao_7_CMV-Forward_D06_04_ABI08.ab1     1 GCGGTCAACC TCCGGCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG   121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC   181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT   241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG   301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG   361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG   421 AGATGGGGCG AGAGCGTCGT ATTAGCGGGG GAAATTAATC GCGATGGGAA AAATCGGTAG   481 GGCAGGGGAA AAAAAATATA ATTAAACTAT AGTATGGCCA GCCGGGAGCA GAACGATTGC   541 GTTAACCTGG CCGGTAAACC ACCAAAGGGT TAACAAATTT GGGAAGTAAC AACTCCCTTC   601 AAAGGGCAAA AAAATTTATT TTTTTTTAAA ACCGGGCCCC CCCCTTTTTT GGGGGGCCCA   661 AGGGGGAAAA AAAAAAAAAC CCCCCGGGGG TTTTTTTTCC CCATTTTGGG GGAAAGCAAA   721 CCCATTTAAA CCCCCCCCCA AAAAAGGGGG CGCCCTTTTT TTTTTAATTT GGGGGGGGGG   781 AAATTTGGGG GGCCATTTTG GGAAAGAATT TTTTTTTAAT ATTAAAAGGG AAAAATTGTC   841 CCCCTTGGGG GGGCCCCCCC CCCCCCGGAA AAAAAAAAAA AGGGGGAAAA AAAAAAAAAA   901 AGGGGGGGAA AAAGGGTTTT TTCTTTGGTG TGTGGGGGAA AAAAAAAAAA TTTGGGGGGG   961 CATAAAATTA ACACAAGAGA GAGAAACAAA ATAAAAATGG GGGGGGGGAG TGCGCCAAAA 1021 AAATTTTTTT TGGGTGTTTT TTGTAGGGGC ACCCCCCTCT TCTTT //
Primer: SV40pro_F Sequence file: RDB16922_A9Aoe.seq
>07722_16922_A9Ao_7_SV40pro_F_E06_05_ABI08.ab1     1 CCCTGGCCCT GGACCTTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG    61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT   121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG   181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG   241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG   301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC   361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC   421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG   481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG   541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG   601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG   661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC   721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG   781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCCTCTA GCTAGAGCTT   841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC   901 ACAACATACG AGCCGGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA   961 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAAG TCGGGAAACC TGTCGTGGCA  1021 GGCTGCCATT AAATGGAATC GGGCCAAACG CCGCGGGGAA GAGGCGGTTT GCCGTAATGG  1081 GGCGCTTCTT CCGCTTCCCT CGCTCACTGA CTCCGCTGCC GCTCGGTCCG TTCGGCTTGC  1141 GGGCGTAGCG GTATACCAGC CTCACTTCAA AAGGGCGTTA ATACGGGTCA TCCCACGGAA 1201 TTCAGGGATA CCGCAGGGAA AGAAACCATG TGTGAAGCTC AACAT //
Primer: EF1a-N_R Sequence file: RDB16922_A9Aof.seq
>07722_16922_A9Ao_7_EF1a-N_R_B07_02_ABI08.ab1     1 TCCCCGGGGG GGGAATGTGG GCGATGTGCG CTCTGCCCAC TGACGGGCAC CGGAGCCTCA    61 CGAAGCTTGA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT   121 TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT   181 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC   241 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT   301 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG   361 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC   421 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG   481 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT   541 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT   601 TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT   661 TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC   721 AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA   781 GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA   841 ACAAATTGTT CTGCTGCTTG CACTATACCA GACAATAATT GTCTGGGCCT GTACCGTCAG   901 CGTCATTGAC GCCTGCGCCC ATAGTGCTTC CTTGCTGCTT CCCAAGAAAC CCAAGGAACA   961 AGCTCCTATT CCCACTGCTC TTTTTTTCTC TCTGCACCAT CTTCTCTTGG CTTGGGTGGG 1021 GTGGCTACTT CAATGGGTTC AATTTTTTAC TACCTTCTTG TTTTATATAA ATTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl