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CS-CDF-EG-PRE-RfA

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Catalog number RDB07722
Resource name CS-CDF-EG-PRE-RfA
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Use DB3.1(Invitrogen) or equivalent to amplify this clone.
Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

References

user report Kusakari, S., Shp2 in forebrain neurons regulates synaptic plasticity, locomotion, and memory formation in mice. Mol. Cell. Biol. 35 (9): 1557-1572 (2015). PMID 25713104.
user report Maruyama, T., Hypothermia-induced tyrosine phosphorylation of SIRP alpha in the brain. J. Neurochem., 121 (6): 891-902 (2012). PMID 22468987.

2018.03.15

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