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CS-UbC-MCS-IRES2-Venus

Backbone vector of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.

Catalog number RDB07696
Resource name CS-UbC-MCS-IRES2-Venus
Clone info. Backbone vector of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-UbC-MCS-IRES2-Venus ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07696 CS-UbC-MCS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07696z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07696.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17872_A9L4p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro-B_F
Region: UbC pro, IRES2
Sequence file: RDB17872_A9L4a.seq check
>07696_17872_A9L4_1_UbCpro-B_F_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNGN GGGGCGGNGA ACGCCGATGN NTATATAAGG
   61 ACGCGCCGGG TGTGGCACAG CTAGTTCCGT CGCAGCCGGG ATTTGGGTCG CGGTTCTTGT
  121 TTGTGGATCG CTGTGATCGT CACTTGGTGA GTAGCGGGCT GCTGGGCTGG CCGGGGCTTT
  181 CGTGGCCGCC GGGCCGCTCG GTGGGACGGA AGCGTGTGGA GAGACCGCCA AGGGCTGTAG
  241 TCTGGGTCCG CGAGCAAGGT TGCCCTGAAC TGGGGGTTGG GGGGAGCGCA GCAAAATGGC
  301 GGCTGTTCCC GAGTCTTGAA TGGAAGACGC TTGTGAGGCG GGCTGTGAGG TCGTTGAAAC
  361 AAGGTGGGGG GCATGGTGGG CGGCAAGAAC CCAAGGTCTT GAGGCCTTCG CTAATGCGGG
  421 AAAGCTCTTA TTCGGGTGAG ATGGGCTGGG GCACCATCTG GGGACCCTGA CGTGAAGTTT
  481 GTCACTGACT GGAGAACTCG GTTTGTCGTC TGTTGCGGGG GCGGCAGTTA TGGCGGTGCC
  541 GTTGGGCAGT GCACCCGTAC CTTTGGGAGC GCGCGCCCTC GTCGTGTCGT GACGTCACCC
  601 GTTCTGTTGG CTTATAATGC AGGGTGGGGC CACCTGCCGG TAGGTGTGCG GTAGGCTTTT
  661 CTCCGTCGCA GGACGCAGGG TTCGGGCCTA GGGTAGGCTC TCCTGAATCG ACAGGCGCCG
  721 GACCTCTGGT GAGGGGANGG ATAAGTGANG CGTCAGTTTC TTTGGTCGGT TTTATGTACC
  781 TATCTTCTTA AGTAGCTGAA GCTCCGGTTT TGAACTATNC GCTCGNGNTT GGCGAGTGTG
  841 TTTTGTGAAG TTTTTTTANG CNNNNTTTGA AATGTANTCA TNTGGGNNNA TATGTAATTT
  901 TTCAGTGTTN NANTANTAAA TTGNCCGCTA AATTCNNGNN NNNTTTTTGG CTTTTTTTNN
961 NTNNNNNNNN NNNNNNNNTN NNCNNNNNNN NNNNNNNNNN ANNNNNNNN
//
Primer: PRE_rev
Region: PRE, Venus 3'
Sequence file: RDB17872_A9L4b.seq check
>07696_17872_A9L4_1_PRE_rev_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNA ANGGNNCNAC NTAGTTAAGA NTACCAGTCA ATCTTTCNCA
   61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG
  121 GCTCGAGAGG CCTTGAATTC TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG
  181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
  241 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG
  301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC
  361 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
  421 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
  481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
  541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
  601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
  661 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
  721 AGCTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGNAC
  781 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGNGAACAGC
  841 TCCTCGCCNN TGCTCACNAT GGNTGTGGNC ATATTATCAT CGNGNTTTTT CAAANGAAAA
  901 CCACGNNCNN NNNGNTCNGG GGGGGCCNAN NNNNTTTTTT NANNTNNANT AAANNCNTNN
961 NANNNATNNG NNNNNNNNGN NNNNNNNNNN NNNNNCNNNN
//
Primer: CMV_Forward
Region: CMV pro, U5, HIV-1 psi
Sequence file: RDB17872_A9L4d.seq check
>07696_17872_A9L4_1_CMV_Forward_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTG NNNCNNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
  121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
  181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
  241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
  301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
  361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
  421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
  481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
  541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
  601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
  661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCNAGGAAG
  721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG
  781 CTGATCTTCA GACCTGGANG NNGANATATG ANGGNNAATT GGAGAAGTGA ATTATATAAA
  841 TATAAAGTAG TAAAAATTGA ANCNTTNNGA NTAGCNCCNN CCNAGGCNAA NANAANNNNN
  901 NNNCANNNAN AAAAAAAGAN CNNNNGGNAN TANGNNCTTN NNNNNNNNGN NTNNNTNNGN
961 NNCNNCNNNA NNNNNNNNNG GNNNN
//
Primer: RRE-M_R
Region: RRE, HIV-1 psi, U5
Sequence file: RDB17872_A9L4c.seq check
>07696_17872_A9L4_1_RRE-M_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CNATAGCCCT CAGCANATTG TTCTGCTGCT
   61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA
  121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT
  181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT
  241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG
  301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT
  361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG
  421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT
  481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG
  541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC
  601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG
  661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC
  721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG
  781 AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT
  841 TCCACACTGA CTAAAAGGGT CTGANGGATC TCTAGTTACC AGANNCACAC AACAGANGGG
  901 CNCACACTAC TNGANNCANT CNAGGNAAGC TTTATTGAGG NTTAANCAGN GGNNTNCNNA
  961 NNNAGCCNGA NNNNNNCNAN NNNCNNNNNT GGNNNNNNNA GANANNNCCA NNANNNNNNA
1021 ANN
//
Primer: cPPT_CTS-E_R
Region: cPPT_CTS
Sequence file: RDB17872_A9L4e.seq check
>07696_17872_A9L4_1_cPPT_CTS-E_R_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNTTTNNNNN NNTCTTTAGT TTGNNNGTCT GTTGCTATTA TGTCTACTAT
   61 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC
  121 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT
  181 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG
  241 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC
  301 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG
  361 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCNATAAT TCTTGTTCAT
  421 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAANGANTG TATTAAGCTT GTGTAATTGT
  481 TNATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGANTNCN AATCTGNTNC ANANATTTAT
  541 TACTNCNNCT ANNNNTCCNA GGNACANCAN NGNNGNNANT GANTTTTCCA NANNNNCNNN
  601 ANNNCCNNNN NNNNNNNGAT NNNTTNNNNN NNNTNNNNNN NNNNNNNNNN NNNNNNNNNN
661 NNNNNNNNNN NCNNNNNNNN
//
Primer: IRES-F
Region: IRES2, Venus 5'
Sequence file: RDB17872_A9L4f.seq check
>07696_17872_A9L4_1_IRES-F_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCNNNNGG NNNNNNNNNG TCTAGGCCCC CCGAACCNCG
   61 GGGNNGTGGT TTTCCTTTGN AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
  121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  241 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  301 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  541 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGANCTAC
  721 CAGTCCGCCC TGAGCAAAGA NCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC
  781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGANCTGT ACAAGTAAGA ATTCNAGGCC
  841 TCTCGAGCCT CTAGAGTTAA CATCGAGGGA TCAAGCTNAT CGATAATCAA CCTCNNGATT
  901 ACNAAATTTG TGAAAGANTG NNNGGTANTN NNNNNNANNN NGNNNCNTTT ACGCNATGNG
961 GNNNNNCNNN NNNNNNNNNN TNNNNNNNNN NNNNTNNNNT NNCNNNN
//
Primer: BGH_rev2
Region: BGH p(A), del U3
Sequence file: RDB17872_A9L4g.seq check
>07696_17872_A9L4_1_BGH_rev2_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGN GGNNGGGCAA NCANCAGNNG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
  121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
  181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
  241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
  301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
  361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
  421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
  481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
  541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
  601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
  661 TACCTGANGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
  721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
  781 ACGGTATCGA TGCGGGGANG CGGCCCNAAG GGANATCCGA NTCGTCTGAN GGCGAANGCG
  841 AANANNCGNA ANANGCCGCA NANCCGGCAG CNNNNCNCGG GNANGNANNN TNCGCNNGNN
  901 TNANGGNCGA ANGGANGNAN CAGAANNNAN NTCNNNCGCA NNNNCCNNNN GGNNNNNCNN
961 NNNANNNNNN NNNNNNAANG NNNNNNNNNN TNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, Zeo
Sequence file: RDB17872_A9L4h.seq check
>07696_17872_A9L4_1_SV40pro_ori_F_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTANGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGNT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
  661 CTCCAGCGCG GGGNTCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTN NNTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
  841 NCNANNNCTA NCTAGAGCTN GNCGNAATCN NGGNCATANC TGTTTCCTGN NNNAAANTGN
  901 TATCCGCNCA NAATTCCACA CNANNTACNA NNNNGNANCN TAAANNNNNA NGCCNNGGNN
961 NNNNNNNNNN NNNNCNNNNN NNNNNTNANN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.12.20

GNP_filter3_RDBDEP_html_191130.pl