Resource data sheet
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CS-UbC-MCS-IRES2-Venus

Backbone vector of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.

Catalog number RDB07696
Resource name CS-UbC-MCS-IRES2-Venus
Clone info. Backbone vector of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-UbC-RfA-IRES2-Venus ()
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


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RDB07696 CS-UbC-MCS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07696z.seq from Depositor
Electronic file PDF RDB07696.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13165_A5C9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F RDB13165_A5C9a.seq
>07696_13165_A5C9_IRES-F_G11_20.ab1
    1 CAGTCTTTCT GTGTTTAGTC GAGGTTAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
   61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC AAGGGCGAGG
  121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
  181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC
  241 TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT
  301 ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
  361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
  421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
  481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
  541 ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA
  601 AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
  661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG
  721 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
  781 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AGAATTCAAG GCCTCTCGAG
  841 CCTCTAGAGT TAACATCGAG GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAATT
  901 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTGCTCCTTT TTACGCTATG TGGATACGCT
  961 GCTTTATTGC TTTGTATCAT GCTATGCTCC CGGTATGCTT TCATTTTCTC TTCTGGATAT
 1021 CTGCTGCTGT CTCTTTATGA GATGTGTCGT TGTCAGGCAA CTGCTGTTGC ACTGGGATTG
1081 CTGAACGCTC ACTCCACTTG G
//
Primer: IRES-R RDB13165_A5C9b.seq
>07696_13165_A5C9_IRES-R_H11_23.ab1
    1 GCGCGCGGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGCGGA TCCGCGGCCG
   61 CTGCAGAATT CTCGAGACCG GTAGCGCTAA ACACGGTCCG GTCTAACAAA AAAGCCAAAA
  121 ACGGCCAGAA TTTAGCGGAC AATTTACTAG TCTAACACTG AAAATTACAT ATTGACCCAA
  181 ATGATTACAT TTCAAAAGGT GCCTAAAAAA CTTCACAAAA CACACTCGCC AACCCCGAGC
  241 GCATAGTTCA AAACCGGAGC TTCAGCTACT TAAGAAGATA GGTACATAAA ACCGACCAAA
  301 GAAACTGACG CCTCACTTAT CCCTCCCCTC ACCAGAGGTC CGGCGCCTGT CGATTCAGGA
  361 GAGCCTACCC TAGGCCCGAA CCCTGCGTCC TGCGACGGAG AAAAGCCTAC CGCACACCTA
  421 CCGGCAGGTG GCCCCACCCT GCATTATAAG CCAACAGAAC GGGTGACGTC ACGACACGAC
  481 GAGGGCGCGC GCTCCCAAAG GTACGGGTGC ACTGCCCAAC GGCACCGCCA TAACTGCCGC
  541 CCCCGCAACA GACGACAAAC CGAGTTCTCC AGTCAGTGAC AAACTTCACG TCAGGGTCCC
  601 CAGATGGTGC CCCAGCCCAT CTCACCCGAA TAAGAGCTTT CCCGCATTAG CGAAGGCCTC
  661 AAGACCTTGG GTTCTTGCCG CCCACCATGC CCCCCACCTT GTTTCAACGA CCTCACAGCC
  721 CGCCTCACAA GCGTCTTCCA TTCAAGACTC GGGAACAGCC GCCATTTTGC TGCGCTCCCC
  781 CCAACCCCCA GTTCAGGCAA CCTTGCTCGC GGACCCAGAC TACAGCCCTT GGCGGTCTCT
  841 CCACACGCTT CCGTCCCACC GAGCGGCCCG GCGGCCACGA AAGCCCCGGC CAGCCCAGCA
  901 GCCCGCTACT CACCAAGTGA CGATCACAGC GATCCACAAA CAAGAACCGC GACCCAAATC
  961 CCCGGCTGCG ACGGAACTAG CTGTGCCACA CCCGGCGCGT CTTAATATAA TCATCGCGTC
 1021 ACCGCCCCCA CGGAGATCCC TCCGCAGATT CGCGAGATGG ACTACTTTCC TCGGCTGGTC
 1081 GCTCTCTGAA GGAAAACCAT GCTAGAGTCA CCTAAGGTCG TCTAAATGTC GATCTGCTGA
1141 ATACT
//
Primer: CMV-Forward RDB13165_A5C9c.seq
>07696_13165_A5C9_CMV-Forward_A12_03.ab1
    1 CTACCTCGCC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAAGC
   61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
  121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
  181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
  241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA
  301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG
  361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG
  421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA
  481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA
  541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG
  601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA
  661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA
  721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT
  781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
  901 GAGAGAAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
  961 AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TTATTGTCTG
 1021 GGTATAGTGC CAGCAGCCAG AACAAATTTG GCCTGAGGGC TATTGAGCGC AACAGCATCT
1081 GATGCCACTT CACAGGTCCT GGGGGCCATC AAGTAGCCTC CCAGAGGGTC A
//
Primer: BGH_rev RDB13165_A5C9d.seq
>07696_13165_A5C9_BGHrev_B12_06.ab1
    1 GTTCGGGGAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
   61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  181 CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG
  241 CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA
  301 GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT
  361 GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG
  421 TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT
  481 TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA
  541 AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT GACTGGAAAA
  601 CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT AGCTGCTGTA
  661 TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG CGGGGAGGCG
  721 GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA
  781 GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG
  841 AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC
  901 AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG
  961 CGGGCAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC
 1021 CCGGGAAGGG AGCTGACAGG TGGTGGGCAT GCCCCACCAG TGGGGGTTGC GTCAGCAACA
 1081 CAGGTGCACA CCACGCCACG TTTGCCTGAC ACCGGACACA CTCCTTCATT AAGGAGGACA
1141 GGCAGCCCAG GATTTATATA CCAACGCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl