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pGL4-phLEP

Promoter collection, Human LEP promoter

Catalog number RDB07688
Resource name pGL4-phLEP
Alternative name U3005-5
Clone info. PCR amplified human LEP promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 128239886 to 128241342(NC_000007.14 ); relatively -1392 to +65, where +1 corresponds to 1 nt of NM_000230.3.
Comment PCR cloning, forward primer: tcaccaacgtcatttgcagt; reverse primer: agcagagaaggaggaaggag. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human leptin genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human leptin

          Reference sequence
            

          Distribution information

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          RDB07688 pGL4-phLEP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07688.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07688_A6C1p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07688_A6C1a.seq check
          >07688_07688_A6C1_2_pGL4-4174F_C04_07.ab1
              1 GTGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 TCACCAACGT CATTTGCAGT TCCCCGCTTT ATGTTATGGG TTTTGTTTTG TTTTGTTTTT
            121 TTTATTGAGA CAGAGTTTCA CTCTTGTTGC CCAGGCTGTA GTGCAATGGT CTGATCTTGG
            181 CTCACTGCAA CCTCCACTTC CCAGGTTCAA GCCATTCTCC TGCCTCAGCC TCTCAAGTAG
            241 CTGGGATTAC AGACACTCAC CACCACACCT GGCTAATTTT GTATTTTTAG TAGAGATGAG
            301 GTTTCACCAT GTTGGCCAGG CTGGTCTCGA AATCCTGACC TCAGGTGATC CACCCACCTT
            361 GGCCTCCCAA AGTGCTGGGA TTACAGGCTT GAGCTACCAC GCCTGGCTGG GTTGGTTCTC
            421 AATGGAGTGG TTTGTTTTTG GAGCTGCTCT GCGCAGTGGG GACCAGAATA GGCCTGGGTT
            481 CCTAGCCCAT TGCTATTCCT TACCAGCTGT GGATTCTAAG GAAAGTCATT TAACCTCGCT
            541 GGACCTTAGA TTCCTCATCC CTGAAGCCCA AGGGTAAAAC AAAACAAAAC AAAACAAAAC
            601 AAACCAACCC ATCATGTAAA GCGGGGAACT ACAAACGAAT ACAGGTGAAA CATGCCTACC
          661 ACACCACTCA CAGGCTATGA TGACAAAAAG TGGGCTACAT CTGGGACCAA CCC
          //
          Primer: pGL4-136R
          Sequence file: RDB07688_A6C1b.seq check
          >07688_07688_A6C1_2_pGL4-136R_D04_10.ab1
              1 GAGGTCTAGT TTGGCATCTT GCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATAGCAGAGA AGGAGGAAGG AGCGCGCCGG GGCCTTACCT
            121 TGCAACCGCT GGCGCTGCGA TTCCTACGGG GCTCCATGCC TGCCCGCCCC TCTTATAGCG
            181 GCCCGATCAC AACTTGCGCA ACTGCCCCGC AGGCCCCGGC GCATTTCTAG CGCCAGCTCC
            241 CGCCCCGCCC CTCAGGGTAG CGACGTGCCG GGCGGCTGCT AGCCCTGGGC CCGCAGTGTG
            301 CACCTCGCGG GGCCTCGAGG GAGGGCCTCG GCGCTCCAGG AGCCACGCGC GCACCCTTGG
            361 GTACCTCCCA AAAATTTGGG GGTGGCTGGT AGGAGCGAGA AATCCTTGAT GTCCCTCCAG
            421 AGGCTCCGGC GCGACTATGG CGCAAGGACC AGCCCCTTGG GAAGGTGCTT CGAGCCGCGC
            481 GGGGCGCGGT CGGGGCGGGG GTGGCTGTGA GGGGCTCCGC GGAGCGGGCT GGGGCATACG
            541 GCTGCGCCCT CGTTGGTCCT GGCGCGGCTT CGGGGTGGGA GCGAATCGGG ACTCGGCCCC
            601 GACGGCCCTG GACGCCAGGG AAGCCCAGGG TTAGAGGGAG CGGCCCGCAG TGCGCCAGGC
            661 TCCCCGACCC AGATTGCGGG GTTGGGGGTT CTGAAGTCCC ACCCCTTCTC TGGCACGCGG
            721 AGAATCGAGT TCCAAGCCTC AGGGCAAGTT GTCTCTGGCT GGCACCCCAG GGAGTGCACA
            781 CTCTCTGGAG GGCTCCAGGC GGGCACTGTG GCCTTTGGGG ACACTCTGGA CATACTGTGG
            841 GTCTCCATCC TCAACTCCGT ATTTCCTACG TACAAAGTGG GGGGTTGGGG GGGGTGGTCC
            901 CAGATGTAGC CACGTTTTTT GTCATCATAG CCTGTGAGTG GTGTGGTAGG CATGTTTCAC
            961 CTGTATCGTT TGTAGTTTCC CCGCTTTACA TGATGGGGTT TGGTTTGTTT TGTTTTGTTT
           1021 TTGTTTTTGT TTTACCCTTT GGGGCTTCAT GGATGAGAAT CTAACGTCCC AGCGAGGGTT
          1081 AAATTGACCT TCGTAGAACT CCACAGCTGG TAAGGAAATT AAGGCCTAT
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07688_A6C1c.seq check
          >07688_07688_A6C1_2_pAxCALNL_F1_E04_13.ab1
              1 TAATTATCTT ATCATGTCTG CTCGAGCGGC CGGCCGCCCC GACTCTAGAA TTATTACACG
             61 GCGATCTTGC CGCCCTTCTT GGCCTTAATG AGAATCTCGC GGATCTTGCG GGCGTCCAAC
            121 TTGCCGGTCA GTCCTTTAGG CACCTCGTCC ACGAACACAA CACCACCGCG CAGCTTCTTG
            181 GCGGTTGTAA CCTGGCTGGC CACATAGTCC ACGATCTCCT TCTCGGTCAT GGTTTTACCG
            241 TGTTCCAGCA CGACGACTGC GGCGGGCAGC TCGCCGGCAT CGTCGTCGGG CAGGCCGGCG
            301 ACCCCGGCGT CGAAGATGTT GGGGTGTTGC AGCAGGATGC TCTCCAGTTC GGCTGGGGCT
            361 ACCTGGTAGC CCTTGTATTT GATCAGGCTC TTCAGCCGGT CCACGATGAA GAAGTGCTCG
            421 TCCTCGTCCC AGTAGGCGAT GTCGCCGCTG TGCAGCCAGC CGTCCTTGTC GATGAGAGCG
            481 TTTGTAGCCT CGGGGTTGTT AACGTAGCCG CTCATGATCA TGGGGCCACG GACGCACAGC
            541 TCGCCGCGCT GGTTCACACC CAGTGTCTTA CCGGTGTCCA AGTCCACCAC CTTAGCCTCG
            601 AAGAAGGGCA CCACCTTGCC TACTGCGCCA GGCTTGTCGT CCCCTTCGGG GGTGATCAGA
            661 ATGGCGCTGG TTGTTTCTGT CAGGCCGTAG CCCTGGCGGA TGCCTGGTAG GTGGAAGCGT
            721 TTGGCCACGG CCTCACCTAC CTCCTTGCTG AGCGGCGCCC CGCCGCTGGC GATCTCGTGC
            781 AAGTTGCTTA GGTCGTACTT GTCGATGAGA GTGCTCTTAG CGAAGAAGCT AAATAGTGTG
            841 GGCACCAGCA GGGCAGATTG AATCTTATAG TCTTGCAAGC TGCGCAAGAA TAGCTCCTCC
            901 TCGAAAGCGG TACATGAGCA CGACCCGAAA GCCGCAGATC AAGTAGCCCA GCGTGGTGAA
            961 CATGCCGAAG CCGTGGGTGA AATGGCACCA CGCTGAGATA GCGGTGTCGG GGATGATCTG
           1021 GGTTGCCGAG ATGGGGGGTC GCGGCATGAC TGAATTCGGA CACAGCCGGT TGCCGGATGC
          1081 GGCTATGCTA CGTCCTTGGG GCCACTCGGC TACCTGCTAC TAATCCTGGG T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl