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pGL4-phCXCL12 (#RDB07687)

Promoter collection, Human CXCL12 promoter

Alternative name U3001-7
Clone info. PCR amplified human CXCL12 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44386550 to 44385073(NC_000010.11); relatively -1445 to +25, where +1 corresponds to 1 nt of NM_199168.4.
Comment PCR cloning, forward primer: gtgggtggatggatggatg; reverse primer: ggctgacggagagtgaaagt. Entire sequence of promoter region has not been confirmed. Known differences (at least) : G to A substitution at 44386558nt. ; G to A substitution at 44386562nt. ; T to C substitution at 44386190nt.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07687.pdf

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RDB07687 pGL4-phCXCL12 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCXCL12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07687).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07687_A5Dnp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: insert 5'
Sequence file: RDB07687_A5Dnc.seq check
>07687_07687_A5Dn_pGL4-4174F_A02_02.ab1
    1 NNNNNNNNNN NNNTNNNNNG GCCNNNCNGG CCNGTNNCTG NNCTCGCTAG CCTCGAGGNN
   61 GTGGGTGGAT GGATGGATGG ATGGATGGAT GGATGGATGG AGTGCCGGCC CACAGCCATC
  121 TAACGGCCAA AGTGGTTTTG GAAAAAAAAT GCACAGAAGA CACCTACTCC CACCAGCGGA
  181 GTTCCGGAGC CCTCGCAGCC TCCTGTTGAC CGCTCCCGCC TAATGCAGCC GCTGACCGCC
  241 CACTCCCCGA CGGCCAGGAC TCCCCAGGGA CAGGGACGTG TCCCCAGGGC AGGCCCCTGG
  301 ATGGACGCGG CGACTGACCC CACTTCGCTG GACGCTGTGC TGGGAAGGAC ACAGAGAGGT
  361 GGCTGGGGCA GCCTGCGGTC ACAAAGCGAG GCCCAAAGGG GCGCTCTCCT CACCCCCACG
  421 CCTCCTGGGT GCCGACCTGC ACCCTCCCTT CGCCACCGGA CTGGGGCCAT CTGGGATGTC
  481 TCGGGGGTAT CCGGAGGGCT AAGCACCGCC GAGGGACGGC TCCGTGGGAA GAGTTTTCTG
  541 GACCCAGAAG GCAGACGCCA GTAGTACTGT CCTAGGAGTC GGAGGTCGGG GTGGGGGAGT
  601 TCTCAGCTCT TTGGGTCGCA CGGAGCTTTT CTTGGGTAAG GCAGTAAGTA CTTAGGTTTA
  661 AAGGACTTAC TTACAGCTAC CATTTATTGA GTACTGTCTG CTTGTCAGAC ACGATGCAGA
  721 GAATTTCGCG GCGTGGGGCG GGTCTCATTG AATCTCCCGT CCCACTCCGC GGGGTGGGCC
  781 TGTGNNTAGC TCATTTCACC ATTGAGANGT CGGAAGTACA AANGCTACAT TCGCTTTTAC
  841 TGANANCCGC CGGCGCCTTC TGCTTTGTTT GTACAGGCGA NGAAACTGAN GNTCGGCTGG
  901 NGGCGCCNNN GGCTNNGANN CCGANCNNCG CNNNNNGCNA NNNNNGGNCN NANNNCCGCA
961 NATCNNNCTN NGCCGGNNNN NNCNCCNCNN NNNNGNNNNN NNGNNNNNNN NNN
//
Primer: pGL4-136R (Pr0079)
Region: insert 3'
Sequence file: RDB07687_A5Dnd.seq check
>07687_07687_A5Dn_pGL4-136R_B02_05.ab1
    1 NNNNNNNNNN NNGGNNNNTT NNNNGGNGGC TTTNNNNACA GTACCGGNNT GCCNAGCTTG
   61 GCCGCCGAGG CCAGATCTTG ATGGCTGACG GAGAGTGAAA GTGCGGCGGT GGGAGGCGCG
  121 CGCCGGGCGC TTTAGAGGGG AGAGCCAGGC GGGGCGGGGC GGGGCGCGCG GTGAGGGGCG
  181 GGCCCGGAGC CGAGTGCTGG CCGTGGCCGG GGTCTCTGAC GCGCGACTCC CTCGGCGGCA
  241 CCCCCCACCC ATCCCCTTCG CCCCTCCTCC GCTCCCTCTG TGCTCCCGAG GCGCAGTGCG
  301 CTCCGGCCTT TGACCTTCTC AGGCTCCGCT GGGCCCCGCA CCGCTGGCCC GGTCTGCAGT
  361 GAGGTCCGCA GCCTGAGCGA CGCCGGCTGG AGGGCCGCTT ATTGTCCCTG TGACAGGGCC
  421 CCACTGGAGA GACTGAGGAC CTTAGGCGTA AAGTGGGGCG CGCGTGGAAA GCTGCGGGAC
  481 GGGGCCTTTC GATGGCGGGA ACTGAATGAG AACCAATGAA AAGCAAACAA GTTAGTCCCG
  541 GGCGGAGGGC CTGCTGGCGG TGGCGGCCCA AGGCAGCCGA GGGGGTCCTG CTTTCTGTGC
  601 GTGGGGGCTC AAGCTCGCCA CCTGCCCGGC TTGCGGCGCA GCCGCCGGCA GAGCTCGATC
  661 TGCGGGAATG AGACCCGTCT TTGCAGTCAG CGTGGCTCGG ACTCCAAGCC CACGGCGCCA
  721 CCAGCCGAGC CTCAGTTTCC TCGCCTGTAC AAACAAAGCA GAANGCGCCG GCGGCTCTCA
  781 NTAAAAGCGA ATGTAGCCTT TGTACTTCCG ANCTCTCAAT GGTGAAATGA NCTNATCACA
  841 GGCCCACCCC GCGGANNGGG ACGGGANNNT CAATGNNNNC CGCCCCACGC CGCGAAATTN
  901 TCNGCATCNN NNCTGANNAN CAGANANNAC TNNATAANNG GNANCTNNAA NTANNNCCTT
961 TNAANNNANN NNNNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 pA,Luc 3'
Sequence file: RDB07687_A5Dne.seq check
>07687_07687_B1FaS_SV40pA_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTAAAGC ATTTTTTTCN CTGCATTCTA GTTGTGGTTT
   61 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT
  121 AGAATTATTA CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT
  181 TGCGGGCGTC CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC
  241 CGCGCAGCTT CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG
  301 TCATGGTTTT ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT
  361 CGGGCAGGCC GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA
  421 GTTCGGCTGG GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA
  481 TGAAGAAGTG CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT
  541 TGTCGATGAG AGCGTTTGTA GCCTCGGGGT TGTTAACGTA NCCGCTCATG ATCATGGGGC
  601 CACGGACGCA CAGCTCGNCG CGCTGGTTCA CACCCAGNGT CNTACCGGTG TNNNAGTNCA
  661 NCACCTTAGC CTCGAANAAN GGNANNACNT TGNNNANNGN NNCNNGNNTN GNNNNCCCCT
721 TNNGGGNNNN NNNNANNNNN NNNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles