RDB07684 - pGL4-phAPOA1

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pGL4-phAPOA1

Promoter collection, Human APOA1 promoter

Catalog number	RDB07684
Resource name	pGL4-phAPOA1
Alternative name	U2914-3
Clone info.	PCR amplified human APOA1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 116839091 to 116837607(NC_000011.10 ); relatively -1141 to +344, where +1 corresponds to 1 nt of NM_000039.2.
Comment	PCR cloning, forward primer: tgaacctgcctgacccttag; reverse primer: ccttctcgcagtctctaagca. Entire sequence of promoter region has not been confirmed. Known differences (at least): G to A substitution at 116,837,697, G to A substitution at 116,838,722 of NC_000011.10.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human apolipoprotein A-I genomic DNA
Depositor\|Developer	DNA Bank, \|

Other clones in our bank

human apolipoprotein A-I

External Database
Human apolipoprotein A-I

Reference sequence

Remarks, protocol and/or map (pdf)	RDB07684.pdf

Distribution information

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Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07684	pGL4-phAPOA1	DNA solution

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How to cite this biological resource

Materials & Methods section:

The pGL4-phAPOA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07684).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Promoter Collection (Firefly Luciferase) (English text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07684_A7Eop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07684_A7Eoa.seq
>07684_07684_A7Eo_pGL4-4174F_D01_04_ABI08.ab1 1 AAGGGGCAGA ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TTGAACCTGC CTGACCCTTA GATTTGAGGT TTTCTGGCTC CAGAATCTTC TCTGAAGTGA 121 AAGGCATGAG GCCGACCACT CCCTGATCTG GTAAACAGAG ATGTCAGCCT GGTTTCTAGT 181 GTTAGGGAGT TTCCTGGAGT GATGGTACAG GGTACATTTC TGCCCTGCAT CCCAAGTCCA 241 GAGACTGGGT TCTAGGTCCA GCCTTTCTTC TAACTCCCTG AGAGATGACA GCCTCTGGGC 301 AAAGTCCCTC TGTGCTCTTC AGTCTCTTCA TCTGTGAGAT GGTGGCATGG GGAGAGGCTG 361 GAGTGATGTC ACAGTTCCTC TCAGCCTGCA TCACAGGCTC TAGGACTCAG GTCCTATCAG 421 TAACCTGCTG AGGGAGGTCT GGGCCTTCCA GGAGAAACCT GACAAGATGG TGCTGCAAAC 481 ACCAACGGAC ACACAGCACT TTACATTCAC AGGCTGTCTC AGGGGCCTCC AACAACCCTG 541 ACCATTCTTG CCCCATTTTG CAGATAGAAA ACCGAGGCTC AGAGAGATTA TATAACTTGC 601 CCACGATCTT CCTCCAGCAA GATGGAGGCC AAGTGAAATG AGAAAGCAGG TCTCCTGCCA 661 CTTCCTTTGC CCAGAGGTCT TCTCCCCACA CCAGGGCTTC CCAAGGGCTG AGATCCAGTC 721 ACACCTGTGC GTGATCAAAT ATAAGTGTGA ACAATGCAAA GGGAGACGTC TTCAATCTAA 781 GGGGCTTCAA TTCTGTAATG TAATTCTGAG ATTATGCCCT TTTTTTGTTA AAGCCTTTCC 841 TTTTTGAAGT GATGGTCACT GTAGATGGTG AGGGTTTTTT TGGAGGCGGA CAATATCTTT 901 ACATGACAAA ATTAAAAGTT GGCAGCTCCG AATTTGATCT CTGGAGTGTT TTGAAATGCA 961 AGAGGGTCTC CGAAACCTCA GTCTGGGAGC CACGGGAGGC TCTCTCTCTC TCAGGGTTTT 1021 ACCTAGGTTT TGGGAGGGCT TTGAGAAGAA GGCCTGAAAG AACTGGCCTG GGGACTAAGG 1081 AGAGCACTTG GTGGAAGGAC CAGGGCGGGG AGGGCGGGAG C //
Primer: pGL4-136R Sequence file: RDB07684_A7Eob.seq
>07684_07684_A7Eo_pGL4-136R_E01_05_ABI08.ab1 1 CTCCTAAATT TTTGGGCATC TTCCATGGTG GCTTTACCAA ACAGTACCGG ATTGCCAAGC 61 TTGGCCGCCG AGGCCAGATC TTGATCCTTC TCGCAGTCTC TAAGCAGCCA GCTCTTGCAG 121 GGCCTATTTA TGTCTGCAGC CAGGGTCTGG GCTGGGAGGC TGATAAGCCC AGCCCTGGCC 181 CTGTTGCTGC TCACTGGTCC TGGCAATGTG GAACTTAAGA GTTCAAGGAT CAGCTCTGTC 241 CCTGGGGCTG GGCAAATAGA GTGGGCAAAC AGCAAGCTGC GGGGGCTGCA GGGCAGGGGT 301 CAAGGGTTCA GTGGGGGCGG GAGGGGAGTG TCTGCAGGCT TGCAGGTCTC CCGGGTGGGG 361 TCGGGGTTCC CTGCACTCAT CCCCTTCCCC TCCATGGGAG TGTGTGGGCA GTTGCCATTG 421 TCCATTGTGT TGGCAGAGGA GGGGAGGGGA GGGACGCTGG GACTCCTCCA CCAAGGAGAC 481 TGCCTCCCCC ACCACCAGCA TTCCAGGGAG ACTACTTCAC TCCCCTCCCC CTTCCCCCGC 541 CCTGTCCTCC CACCAGTGCT CTTCTTTAGT CCCCAGCAGG TCCTCCAGGC CTCTCTCCAA 601 GCCTCCCAAA CTGGTAAACC TGGGGAGAGG GGAGAGCCCT CCGTGGCTCC CAGACTGAGG 661 TTTCGGAGAC CTCTTGCATT TCAAAACACT CCAGAGATCA ATTCGGAGCT GCCAACTTTT 721 AATTTTGTCA TGTAAAGATA TTGTCCGCCT CCAAAAAACC CTCACCATCT ACAGTGACCA 781 TCACTTCAAA AAGGAAAGGC TTTAACAAAA AAGGGCATAA TCTCAGAATT ACATTACAGA 841 ATTGAAGCCC CTTAGATTGA AGACGTCTCC CTTTGCATTG TTCACACTTA TATTTGATCA 901 CGCACAGGTG TGACTGGATC TCAGCCCTTG GGAAGCCCTG GTGTGGGGAG AAAGACCCTC 961 TGGGCAAAGG AAGTGGCAGG AGACCCTGCT TTCTCATTTC ACTTTGGCCT CCAATCTTGC 1021 TTGGAGGAAG ATCGTTGGGG CAAGGTTATA ATAATCTCTC TGAAGCCTCG GATTTCTATC 1081 TGGCAAATGG GGCAAGAAAT TGATCCATAG //
Primer: pAxCALNL_F1 Sequence file: RDB07684_A7Eoc.seq
>07684_07684_A7Eo_pAxCALNL_F1_F01_06_ABI08.ab1 1 TTAATGTTAT CTTATCATGT CTGCTCGAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC 61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC 121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC 181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA 241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG 301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG 361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC 421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA 481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC 541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC 601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC 661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG 721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG 781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT 841 GTGGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC 901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT 961 GAACATGCCC GAAGCCGTGG TGAAATGGCA CCACGCTGAG ATAGCGGTGT CGGGGGATGA 1021 ATCTGGCTGC TGAGATGGGG GTCGCGGCAT GACTGGATTC GGAACCAGCG CTGCGGATGC 1081 CGCTAGGCCT ACGCTCTCGT ATGGGGCCAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.09

GNP_filter3_RDBDEP_html_231027.pl