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pcDNA3.1(-)-h Integrin beta3 (#RDB07663)

Clone info. Expression vector of human integrin beta3
Comment cDNA from BC127666.
Vector backbone pcDNA3.1(-) (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human integrin beta3 cDNA
Depositor|Developer Hamada, Hirofumi |
 

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07663 pcDNA3.1(-)-h Integrin beta3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(-)-h Integrin beta3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07663).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07663_B2Lqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'
Sequence file: RDB07663_B2Lqa.seq check
>07663_07663_B2Lq_3_CMV-Forward_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGGGA GNNNNNNATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC
   61 ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG
  121 CGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCCAGTGT GATGGATATC TGCAGAATTC
  181 GCCCTTCGCC GCGGGAGGCG GACGAGATGC GAGCGCGGCC GCGGCCCCGG CCGCTCTGGG
  241 CGACTGTGCT GGCGCTGGGG GCGCTGGCGG GCGTTGGCGT AGGAGGGCCC AACATCTGTA
  301 CCACGCGAGG TGTGAGCTCC TGCCAGCAGT GCCTGGCTGT GAGCCCCATG TGTGCCTGGT
  361 GCTCTGATGA GGCCCTGCCT CTGGGCTCAC CTCGCTGTGA CCTGAAGGAG AATCTGCTGA
  421 AGGATAACTG TGCCCCAGAA TCCATCGAGT TCCCAGTGAG TGAGGCCCGA GTACTANAGG
  481 ACAGGCCCCT CAGCGACNAG GGCTCTGGAN ACAGCTCCCA GGTCACTCAA GTCAGTCCCC
  541 AGAGGATTGC ACTCCGGCTC CGGCCAGATG ATTCNAANAA TTTCTCCATC CAAGTGCGGC
  601 AGGTGGAGGA TTACCCTGTG GANNNNNNCT ACTTGATGGA CCTGTCTTAC TCCNNNNNNG
  661 ATGATCTGTG GAGCATCCAN AACCTGGGTA CCANNCTGGC CACCCANATG CGAAAGNNNN
  721 NNANTANCCT GCGGATNGNN NNCNGGGGCA TTTGTGGANN AANCCTGTGT NACNNTACAT
  781 GNNNNTCTCC CCCACCNNAN GNCNNCNAAA ACCNCTGNNA TGATATGAAN NACNNCCTGN
841 NTGNNNNNGT TNNGNNTANN NNCNNNNGNT GNNNCTNACT NNNNNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,insert 3'
Sequence file: RDB07663_B2Lqb.seq check
>07663_07663_B2Lq_3_bGH_rev3_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNACTNNAAG GNACAGTCGA GGCTGATCAG CGGTTTAAAC
   61 TTAAGCTTGG TACCGAGCTC GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCGCC
  121 CTTTGCTTTT ACTCAGCTTA TCAGGACACA TCACAATTTT AAATTACACA CTCCCACACA
  181 CAACACACAC ACATAAACAC ACAGACCCCC ACATACACAC ACACAGACCC ACACTTCCAC
  241 ATACTGACAT TCTCCCAACC TACCCCACTC TGAGCCCCAA ATCCCTCCCC NNNNNTACTG
  301 TCCTCTGTAA ACATGATGGC AGGGACTCCT GCAATCGTGG CACAGGCTGA TAATGATCTG
  361 AGGATGACTG CTTATCATTA AGTGCCCCGG TACGTGATAT TGGTGAAGGT AGACGTGGCC
  421 TCTTTATACA GTGGGTTGTT GGCTGTGTCC CATTTTGCTC TGGCGCGTTC TTCCTCAAAT
  481 TTAGCGAATT CTTTTCGGTC GTGGATGGTG ATGAGGAGTT TCCAGATGAG CAGGGCGGCA
  541 AGGNCNNNGA GCANAATGGC CCCCATCACT GANAGCANGA CCACCANNNN NNCANGGCCC
601 TTGGGACACT CTGGCTCTTC TANNNNNTAC AGGATGGANT TNNNNNNNNN N
//
Primer: hIntegrin-b3-B_F (Pr1283)
Region: insert mid
Sequence file: RDB07663_B2Lqc.seq check
>07663_07663_B2Lq_3_hIntegrinb3-B_F_B01_04_ABI24.ab1
    1 NNNNNNCNTN NNGAAAANAC CNNNAACCGT TACTGCCGTG ACGAGATTGA GTCAGTGAAA
   61 GAGCTTAAGG ACACTGGCAA GGATGCAGTG AATTGTACCT ATAAGAATGA GGATGACTGT
  121 GTCGTCAGAT TCCAGTACTA TGAAGATTCT AGTGGAAAGT CCATCCTGTA TGTGGTAGAA
  181 GAGCCAGAGT GTCCCAAGGG CCCTGACATC CTGGTGGTCC TGCTCTCAGT GATGGGGGCC
  241 ATTCTGCTCA TTGGCCTTGC CGCCCTGCTC ATCTGGAAAC TCCTCATCAC CATCCACGAC
  301 CGAAAAGAAT TCGCTAAATT TGAGGAAGAA CGCGCCAGAG CAAAATGGGA CACAGCCAAC
  361 AACCCACTGT ATAAAGAGGC CACGTCTACC TTCACCAATA TCACGTACCG GGGCACTTAA
  421 TGATAAGCAG TCATCCTCAG ATCATTATCA GCCTGTGCCA CGATTGCAGG AGTCCCTGCC
  481 ATCATGTTTA CAGAGGACAG TATTTGTGGG GAGGGATTTG GGGCTCAGAG TGGGGTAGGT
  541 TGGGAGAATG TCAGTATGTG GAAGTGTGGG TCTGTGTGTG TGTATGTGGG GGTCTGTGTG
601 TTTATGTGTG TGTGTTGTGT GTGGGAGTGN NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB07663_B2Lqd.seq check
>07663_07663_B2Lq_3_SV40pro_ori_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC
   61 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC
  121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
  181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
  241 GCGCAGGGGC GCCCGGTTCT TTTTNNNNNG ACCGACCTGT CCGGTGCCCT GAATGAACTG
  301 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
  361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
  421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
  481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC NNNNNNTCNC
541 NTCGAGCGAG CACGTACTCG GATGGAAGCC NNNNNNGTCG ATCAGGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.