Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1(-)-h Integrin alphaV (#RDB07660)

Clone info. Expression vector of human integrin alphaV
Comment cDNA from BC126231.
Vector backbone pcDNA3.1(-) (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human integrin alphaV cDNA
Depositor|Developer Hamada, Hirofumi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07660 pcDNA3.1(-)-h Integrin alphaV DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(-)-h Integrin alphaV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07660).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07660_B2Lqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'
Sequence file: RDB07660_B2Lqa.seq check
>07660_07660_B2Lq_1_CMV-Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNT ANGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG
  181 CCAGTGTGAT GGATATCTGC AGAATTCGCC CTTCTAGCCG AGAAGAGAGC GGCCGGCAAG
  241 TTTGGGCGCG CGCAGGCGGC GGGCCGCGGG CACTGGGCGC CTCGCTGGGG CGGGGGGAGG
  301 TGGCTACCGC TCCCGGCTTG GCGTCCCGCG CGCACTTCGG CGATGGCTTT TCCGCCGCGG
  361 CGACGGCTGC GCCTCGGTCC CCGCGGCCTC CCGCTTCTTC TCTCGGGACT CCTGCTACCT
  421 CTGTGCCGCG CCTTCAACCT AGACGTGGAC AGTCCTGCCG AGTACTCTGG CCCCGAGGGA
  481 AGTTACTTCG GCTTCGCCGT GGATTTCTTC GTGCCCAGCG CGTCTTCCCG GATGTTTCTT
  541 CTCGTGGGAG CTCCCAAAGC AAACACCACC CAGCCTGGGA TTGTGGAAGG AGGGCAGGTC
  601 CTCAAATGTG ACTGGTCTTC TACCCGCCGG TGCCAGCCAA TTGAATTTGA TGCAACAGGC
  661 AATAGAGATT ATGCCAAGGA TGATCCATTG GAATTTAAGT CCCATCAGTG GTTTGGAGCA
  721 TCTGTGAGGT CGAANCNNGA TAAAATTTTG GCCTGTGCCC CATTGTACCA TTGGAGAANN
  781 TGANATGAAA CAGGANCGAG AGCCTGNTTG GAACATGCTT TCTTCNAGAT GGANNAAAGA
  841 CTGTTGAGTA NGCTCCNTGT ANNTCNNNAN NANATTTGAT GCTGANGGNN NNGGANTTTG
  901 TCNANGNNNG NNNNNNCNNT GATNNNNNNN NAAGCTNNNN GANNNNTTCN TGGNNNNNCC
  961 NTGNNNNNCN TNTNNNTGNN AANNGNCANC NTNNTTTCNN NNNTCANNTG GNNNAANNNN
1021 NNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,insert 3'
Sequence file: RDB07660_B2Lqb.seq check
>07660_07660_B2Lq_1_bGH_rev3_B01_02_ABI08.ab1
    1 GNNNNNNNNN NNNNNNNNNN NNNNNNGNCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG
   61 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC AGTGTGCTGG
  121 AATTCGCCCT TTAAATGGTG GGGTAAGGGG TCCAGGTTCC TGTCAACTGT AAAGATTTCT
  181 CACTGATCTT GACCCATTCT CAGTAACAGT TTTGGAAATA TAAGGAAAAG TACATGAATG
  241 GAGGAAAATA AATTAATACT AACTAAAAAC TCTGCTCTAC TCTAAGAATG TAATACTATA
  301 TGCATAAGCA ACTATCTCAA AGAACTACAC AGTATTTTAA TAAAAATGCT TTTTTTATAC
  361 TGTAAATAGC CAGACTAAAA CAATGGAATA AATATGCTTT GGAAATAATT TCCAACTCCA
  421 TATGGCTTTA AGCATTATGA TATTCAGGTG AAATTAAACA ATTACACAAA AGAGCCATTT
  481 GTGATTATGG AACTAATTTT TAGTATAGGT TGGCTTGAAG GATTTGACCT AAAATTTATA
  541 CAAGTTAAAA ATGTAATAAA ATATTGTGGA TTTAATATAA ACAGAAGTGT AATAGGATCT
  601 ACCAGATTTT CATACTAAAA CAAAATATTG TACCTTTGTT ATTAAAAGCA AGAATTTGGA
  661 TGGTATCATG TATGTGTTTA TAAAATTCTA CCATATGAGT GATTAAAAAA ATCCAAGTTC
  721 ATGCAGTTAT AAAGTCTTTN NTATTCTGAA AAGTAAACTG TAGTGTAATA TGTATTAACA
  781 TTAAGTACCT ATACAAAANA CATGATTGCN NAAGGTCCAA ATTTGGANTG CTTTAAAACA
  841 ACAACAACAA CAACAACNAA CAANNACAAA TAAANGTTCC GNNNCNTTNA AATCNNAAAN
  901 NAGNNNNTGN ANGGAGGTAC NNNNTTTTAT ANNNANGNAN NNAANTCANG NACNNNCCTG
  961 TNNTNANNNN NNNNNTNNAN NNNNNAACNA TNNNNTNNAA NNTTTNANTN NNNNNAANNN
 1021 NNNAAAANNN CNAGTNNNNC NNNNNGTNNN NNNANNNGNA NNNNNNNNCN NNNNTNNNNN
1081 NNNNN
//
Primer: hIntegrin-aV-B_R (Pr1283)
Region: insert mid
Sequence file: RDB07660_B2Lqc.seq check
>07660_07660_B2Lq_1_hIntegrinaV-B_R_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN ATAATAAANT TGCTAATTCT AGTGGGTCAA GATGTAGCAT
   61 AACTTAAAAA CTGCAGTTAA GTTTCTGAGT TTCCTTCACC ATTTTCATGA GGTTGAAGCT
  121 GCTCCCTTTC TTGTTCTTCT TGAGGTGGCC GGACCCGTTT AAAAAAGCCC ATCCTGTACA
  181 TTACAAATAC CAAAACAGCC AGTAGCAACA ATCCTGCTAG AACTGCTAAA ATGATCACCC
  241 ACACAGGCAC AGGCATGGGC GCTGGCTGAA TGCCCCAGGT GACATTAGTG GTAACCAATG
  301 TGGAGTTGGT GATATCCTCA ATTGGAAGAT TCTTATAAGG AAACTCTATG ACATTAAATG
  361 AAGCAGACGA CTTCAGAGAA TAGGAATGAT TCTGATTTTC TTTATTCATA AAAGTCTCAG
  421 TCCACAGTAA TGACTTTACG TACAAGATTG CACTCTTTCC TCTGTCTAAT CTCCCAACTT
  481 GGCAGACAAT CTTCAAGCAC TGAGCAACTC CACAACCCAA AGTGTGAATA TCTCCTTCAC
  541 TGAGGGCAAG ATCCCGCTTA GTGATGAGAT GGTCCCGCTC ACCTTGCCCG GCAACCGTGT
  601 CATTCTTTTC AGTTGTTTGC AAAGATGAGA TCTTAATTCT CAAAGGGTTG ATCTCCATAT
  661 CTGAAGTGCA GTTCATTGGT CCATCAATAT CATAATGAAG GANATACAAC AGAGTGTTAT
  721 TATTATATTT GTAAGGCCNC TGAAGATGGA GCATTGCCTT GCTGAATGAA CTTGGACCAT
  781 TGTTTCTCAG CTCATAGATG TGCTGAACAA CTGGCCCNAC ATCTTCTTCA GTCTCAGGGT
  841 TCTCCTTGTG CTCCCAGTTT GNAATCNGAA GAAAGANATG ATCAGGACTC NAGANTNCTC
  901 TTATCTCANN TGNAGCTNAN NAGCNNNATC NACTTTGTGA GANACNANNG GNNNTACTTT
961 GTNAANANAN TTNANCTTNG GNNNNNNNGN NAANNTTCNC NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB07660_B2Lqd.seq check
>07660_07660_B2Lq_1_SV40pro_ori_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTANT NNNNNNNTAG TGAGGAGGCT TTTTTGGAGG
   61 CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA
  121 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
  181 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
  241 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT
  301 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC
  361 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT
  421 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT
  481 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT
  541 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT
  601 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG
  661 GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT
  721 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCNACTGTG GCCGGCTGGG
  781 TGTGGCGGAN CNNNNNNAGG ACATAGCGTT GGCTANCCCG TGATANTGCT GANNANCTTG
  841 GCGGCGANNG GGCTGACCGC TNCCTCGTGC TTTACGGTAT CNCCGCTCCC GANTCNCNNN
  901 GCATCNCCTT CTATCGCCTT CTTGACNANT TCNNCTGANN CNGNNNTCTN GGGGTNCGAA
  961 ATGNNNNANN NNNNGACGCC NNANNCTGNC CNTNNCNANN NTNNNATTCC ACCNNNNNNC
 1021 CTNCTATGAA ANNNNNNGGN NNTNNNGANT NNNNTTNTNC NGGGNNNNNC NGNNNNNNNN
1081 GANNCNNNNN NNNNCNGGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.