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pcDNA3.1(+)cs-h Integrin alpha5 (#RDB07658)

Expression vector of human integrin alpha5.

Clone info. Expression vector of human integrin alpha5.
Vector backbone pcDNA3.1(+)cs (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human integrin alpha5 cDNA
Depositor|Developer Hamada, Hirofumi |
 

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07658 pcDNA3.1(+)cs-h Integrin alpha5 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(+)cs-h Integrin alpha5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07658).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07658_A9Kbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,attB1,I-CeuI,insert5'
Sequence file: RDB07658_A9Kba.seq check
>07658_07658_A9Kb_2_CMV-Forward_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNNGGGCGG TANGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA
  181 TCCACAAGTT TGTACAAAAA AGCAGGCTTG TAAAACGACG GCCAGTAACT ATAACGGTCC
  241 TAAGGTAGCG AGGCCTGGGT GGCGAATTCG GCACGAGGGT TTAGGAAGCG GCTCCGGGTC
  301 GGTGGCCCCA GGACAGGGAA GAGCGGGCGC TATGGGGAGC CGGACGCCAG AGTCCCCTCT
  361 CCACGCCGTG CAGCTGCGCT GGGGCCCCCG GCGCCGACCC CCGCTGCTGC CGCTGCTGTT
  421 GCTGCTGCTG CCGCCGCCAC CCAGGGTCGG GGGCTTCAAC TTAGACGCGG AGGCCCCAGC
  481 AGTACTCTCG GGGCCCCCGG GCTCCTTCTT CGGATTCTCA GTGGAGTTTT ACCGGCCGGG
  541 AACAGACGGG GTCAGTGTGC TGGTGGGAGC ACCCAAGGCT AATACCAGCC AGCCAGGAGT
  601 GCTGCAGGGT GGTGCTGTCT ACCTCTGTCC TTGGGGTGCC AGCCCCACAC AGTGCACCCC
  661 CATTGAATTT GACAGCAAAG GCTCTCGGCT CCTGGAGTCC TCACTGTCCA GCTCAGAGGG
  721 ANAGGAGCCT GTGGAGTACA AGTCCTTGCA GTGGTTCGGG GCAACAGTTC GAGCCCATGG
  781 CTCCTCCATC TTGGCATGCG CTCCACTGTA CAGCTGGCGC ACANNNAANG ANCCACTGNN
  841 NGACCCCGNG GGNCACCNGC TACNTCTNCA CANNTAANTT CACCCNAATT CTGNNNTATG
  901 CACCCNGCNG CNCANNNTTC ANCNNGNNNN CNNNACAGGN NNNNNGNCNA NNNNNGNNNN
961 NNNNNN
//
Primer: bGH-M_R (Pr0747)
Region: bGHp(A),attB2,PI-SceI,insert3'
Sequence file: RDB07658_A9Kbb.seq check
>07658_07658_A9Kb_2_bGH-M_R_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN GGGCNNNCAA CAGNTGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCCAC TGTGCTGGAT
  121 GGGTACTGCA GCCGATTCAT TAATGCAGGT TAACCTGGCT TATCGAAATT AATACGACTC
  181 ACTATAGGGA GACCGGCAGA TCTACCACTT TGTACAAGAA AGCTGGGTCA GGAAACAGCT
  241 ATGACCATGT GCCATTTCAT TACCTCTTTC TCCGCACCCG ACATAGATGC CTCGAGTTTT
  301 TTTTTTTTTT TTTTGGTCAG TGGGGGCACT GGGTCANATN NATTGCACTT GCAACANANT
  361 TTAAATAANT CCTGGGTGTC TGGTGCCAAG GTGAGGGAAG GGTTGGGCAN ANANATGAGG
  421 GGCAGCATCA NTGCAGCTGG CAGGCANAAC CCAAATTCTG CAGGCCCAGG ACAGTGGGCT
  481 CCCCTTTCTC TGGGGAACAG GGAGGGCCTG TGTCTGGCCA GGCTGANGTT CCANATNNGT
  541 TGCCATCATG GCCCCTTCNN GGTCCTGGGA AATTCCTGGC TTCTCCTAAA TCANGGTGAA
  601 CTGGGCCTCC AGNATCANGT CTGGAGCANG CCCAAATATA GTCCTGGATC TGCCTGGATT
  661 ANGTGCCAAT GTCTGANTCT GGGTTCCANA TNNACTCCNG ACNCCAGGCT GGATCTGNNC
  721 CCATTTGAGT TCTGATTCCC CTNGGANCTG GGCTCTGGGC CCTGGGCCAG CATCTATCCN
  781 TGTGTGGNAT CTGTCCTGAN CTGGTCCTGG GGCANCNTGC ATAGTTAGTG NTCTTTGTTG
  841 GCCCNCCAGG GNGGGGNTGT NNNGANCTGN NNNGNNNTNN NNNNNNNAGT TNCNNNNNNN
901 TANNNNGGGG NNNNNTTTGG NTNTC
//
Primer: hITGA5-B_F1 (Pr0777)
Region: insert3'
Sequence file: RDB07658_A9Kbc.seq check
>07658_07658_A9Kb_2_hITGA5-B_F1_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NAGNNGCTGG CCCNGGGCCC NGNNCCCAGC TCCNAGGGGA
   61 ANCAGAACTC AAATGGGGCC AGATCCAGCC TGGGGTCTGG AGTTGATCTG GAACCCAGAC
  121 TCAGACATTG GCACCTAATC CAGGCAGATC CAGGACTATA TTTGGGCCTG CTCCAGACCT
  181 GATCCTGGAG GCCCAGTTCA CCCTGATTTA GGAGAAGCCA GGAATTTCCC AGGACCCTGA
  241 AGGGGCCATG ATGGCAACAG ATCTGGAACC TCAGCCTGGC CAGACACAGG CCCTCCCTGT
  301 TCCCCAGAGA AAGGGGAGCC CACTGTCCTG GGCCTGCAGA ATTTGGGTTC TGCCTGCCAG
  361 CTGCACTGAT GCTGCCCCTC ATCTCTCTGC CCAACCCTTC CCTCACCTTG GCACCAGACA
  421 CCCAGGACTT ATTTAAACTC TGTTGCAAGT GCAATAAATC TGACCCAGTG CCCCCACTGA
  481 CCAAAAAAAA AAAAAAAAAN NTCNAGGCAT CTATGTCGGG TGCGGAGAAA GAGGTAATGA
  541 AATGGCACAT GGTCATAGCT GTTTCCTGAC CCAGCTTTCT TGTACAAAGT GGTAGATCTG
  601 CCGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC AGGTTAACCT GCATTAATGA
  661 ATCGGCTGCA GTACCCATCC AGCACAGTGG CGGCCGCTCG AGTCTANAGG GCCCGTTTAA
  721 ACNCGCTGAT CAGCCTCNAC NGTGCCTTCN ANTTGCCAGC CNTCTGTTGT TTGCCCCTCC
  781 CCCGNGCCTT CCTTGACCCN NNNANGGNGN CNCTCCCACT NNNCTTTNNN NNNNAAANNA
  841 NNAAANTGCN NCNNNNNNNC NNNNTNNNNN NCNNTNNNNN CTGGGGGNNN NGGGNNNNGG
901 GNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB07658_A9Kbd.seq check
>07658_07658_A9Kb_2_SV40pro_ori_F_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CTGCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA NGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
  541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
  601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
  661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGACCC ATGGCGATGC
  721 CTGCTTGCCG AATATCATGG TGGANNATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG
  781 GCTGGGTGTG GCGGANCGCT ATCAGGACAT AGCGTTGGCT ACCCGNGATA TTGCTGAAGA
  841 NCTTGGCGGC GAANGGGCTG NNCGCTTCCT CGNGCTTTAC NGNATCGCCG CNNCNNANNC
  901 GCANCGCATC GCCNTCNANN GCNNNNNNNN CNANNNCTTN NNANCGGGNN NNNNGGGNNN
961 NNAANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.