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pcDNA3-hRAB39B (#RDB07654)

Vector backbone pcDNA3.1D/V5-His-TOPO (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human RAB39B cDNA
Depositor|Developer Hamada, Hirofumi |
 

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07654 pcDNA3-hRAB39B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-hRAB39B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07654).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07654_B1D8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB07654_B1D8a.seq check
>07654_07654_B1D8_3_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGG TACCGAGCTC GGATCCAGTA
  181 CCCTTCACCA TGGAGGCCAT CTGGCTGTAC CAGTTCCGGC TCATTGTCAT CGGGGATTCC
  241 ACAGTGGGCA AGTCCTGCCT GATCCGCCGC TTCACCGAGG GTCGCTTTGC CCAGGTTTCT
  301 GACCCCACCG TGGGGGTGGA TTTTTTCTCC CGCTTGGTGG AGATCGAGCC AGGAAAACGC
  361 ATCAAGCTCC AGATCTGGGA TACCGCGGGT CAAGAGAGGT TCAGATCCAT CACTCGCGCC
  421 TACTACAGGA ACTCAGTAGG TGGTCTTCTC TTATTTGACA TTACCAACCG CAGGTCCTTC
  481 CAGAATGTCC ATGAGTGGTT AGAAGAGACC AAAGTACACG TTCAGCCCTA CCAAATTGTA
  541 TTTGTTCTGG TGGGTCACAA GTGTGACCTG NATACACAGA GGCAAGTGAC TCGCNACGAG
  601 GCCGAGAAAC TGGCTGCTGC ATACGGCATG AANTACATTG AAACGTCNGC CCGANNNGNC
  661 ATTNNTGNGG AGAANNNCTT CNNNGNNCTG ANNNGAGANN TNTATNANNT NNNTAAANNG
721 GGGNNNNNNN NNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,His tag,V5 tag,insert 3'
Sequence file: RDB07654_B1D8b.seq check
>07654_07654_B1D8_3_bGH_rev3_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACTCAATG GTGATGGTGA TGATGACCGG TACGCGTAGA
  121 ATCGAGACCG AGGAGAGGGT TAGGGATAGG CTTACCTTCG AACCGCGGGC CCTCTAGACT
  181 CGAGCGGCCG CCACTGTGCT GGATATCTGC AGAATTGTCT TGACCCTTCT AGCACAAACA
  241 TCTCCTCTCT GATTTGACAA CCTCTTCTGA AGAGTGAACC ACATTTGGTA CAAATCCACT
  301 CTTCACCCCT TCCCAGCCCT CCTGGATTGT AATCTCCCCC CTTTTAACCA GCTCATATAT
  361 GTCTCTTGTC AGGTCTGTGA AGGCTTTCTC CACATTAATG GCATCTCGGG CTGACGTTTC
  421 AATGTACTTC ATGCCGTATG CAGCAGCCAG TTTCTCGGCC TCGTGGCGAG TCACTTGCCT
  481 CTGTGTATCC AGGTCACACT TGTGACCCAC CAGAACAAAT ACAATTTGGT AGGGCTGAAC
  541 GTGTACTTTG GTCTCTTCTA ACCACTCATG GACATTCTGG AANGACCTGC GGTTGGTAAT
  601 GNCAAATAAG AGAAGANCAC CTACTGAGTT CCTGTAGTNN NGCGAGTGAN GGANCTGNAC
  661 CTCTCTTGAN CCGCGGTANC CCNGATCTGG ANCNNGNNGC NNTTTNNNNN NNCNNTNNNN
721 NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB07654_B1D8c.seq check
>07654_07654_B1D8_3_SV40pro_ori_F_H02_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNGCCGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GANGGAAGCC
  601 GGTCTTGTCG ATCANGANGA TCTGGACNAA GAGCATCNNG GGCTCGCGCC NGCCNAACTG
  661 NTCGCNNGGC TCNNNNNNCG NATGCCCNAN NGNNANNNNC TCNTCNNGNN CCNTGGCGNN
721 NNNNGCTNNN NNANNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.