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pcDNA3.1(-)-hIGF1R (#RDB07648)

Expression vector of human IGF1R cDNA.

Comment cDNA from BC113612.
Vector backbone pcDNA3.1(-) (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human IGF1R cDNA
Depositor|Developer Hamada, Hirofumi |
 

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07648 pcDNA3.1(-)-hIGF1R DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(-)-hIGF1R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07648).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07648_B2Dsp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert5'
Sequence file: RDB07648_B2Dsa.seq check
>07648_07648_B2Ds_1_CMV_Forward_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNNANG CNNGTNCNGN GGGANGNCTN
   61 NNNNNNCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACGGGCCCT CTAGACTCGA
  181 GCGGCCGCCA CTGTGCTGGA TATCTGCAGA ATTCGCCCTT TGAGAAAGGG AATTTCATCC
  241 CAAATAAAAG GAATGAAGTC TGGCTCCGGA GGAGGGTCCC CGACCTCGCT GTGGGGGCTC
  301 CTGTTTCTCT CCGCCGCGCT CTCGCTCTGG CCGACGAGTG GAGAAATCTG CGGGCCAGGC
  361 ATCGACATCC GCAACGACTA TCAGCAGCTG AAGCGCCTGG AGAACTGCAC GGTGATCGAG
  421 GGCTACCTCC ACATCCTGCT CATCTCCAAG GCCGAGGACT ACCGCAGCTA CCGCTTCCCC
  481 AAGCTCACGG TCATTACCGA GTACTTGCTG CTGTTCCGAG TGGCTGGCCT CGAGAGCCTC
  541 GGAGACCTCT TCCCCAACCT CACGGTCATC CGCGGCTGGA AACTCTTCTA CAACTACGCC
  601 CTGGTCATCT TCGAGATGAC CAATCTCAAG GATATTGGGC TTTACAACCT GAGGAACATT
  661 ACTCGGGGGG CCATCAGGAT TGAGAAAAAT GCTGACCTCT GTTACCTCTC CACTGTGGAC
  721 TGGTCCCTGA TCCTGGATGC GGTGTCCAAT AACTACATTG TGGGGAATAA GCCCCCAAAG
  781 GANNGTGGGG ANCTGTGTCC AGGGNNCATG GGAGGAGAAG CCGANGTGTG ANAAGANCNN
  841 NNTCAACNAN TGAGTACAAC TACCGCTGCT GGACCANNAA CCGCTGCCAG AAAATGTGCC
  901 CNAGCACGTG TGGGNAAGCN GGGCGTGCAC CGAGAACNAT GAGTGCTGNC NCCCCCGANT
  961 GNCTGGGCNN CTGNNGCNCG CNTGANNNCN ACNNNGGCCT GNNNAGCNTG NCNNNNCTAC
 1021 TNNNANNNCC GGNNNNNNGT NNNNNNNNNC NNNNNNNNCC NNNNCCNNCN NNNTTNNNNN
1081 GNNNNGGNNN NNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: insert 3'
Sequence file: RDB07648_B2Dsb.seq check
>07648_07648_B2Ds_1_bGH_rev3_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGNNG NNGGNNNNNN ACNGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGNTC NGCGGTTTAA ACTTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG
  121 TGGTGGAATT CGCCCTTAAC ACTTGGGCTT CCCAGCTGGA CTTGTGGCAA TTATTTTTCC
  181 GTTATGAAGC AGAGAGGAGA GAGAAAGGGA GAACAGGGAA GGACAGGGAC AGAGTGAGGA
  241 GACTGGTTCT CAAGTGCTCT GTTGCTATTA AGTGTTGTCA TTAAGGTTCT TAAAGGCCCA
  301 TGTCAGTTAA GGGTTTGGGC AGGGAATAAA AAGCTGCTTG CATATTGAAA AAAGGAACAT
  361 CCCAAATGTG TTTTACTGCA GAGAAGCTGT CTCCCGCGGG CAGCAAGGGC AGTTCTGAAG
  421 ATCCACTGAG GTACAGGAGG CTTGTGAATG GATTGTTAAA ACTCTCTCTC CCCCCCCACC
  481 CCGCTGCGCG TGCGCACACG TTACTGTTTG CACAGATTCA GGATCCAAGG ATCAGCAGGT
  541 CGAAGACTGG GGCAGCGGCA AGGCCCGCTC GTTCTTGCGG CCCCCGTTCA TGTGGGCGTA
  601 AGGCTGTCTC TCGTCGAAGC TGGCGCGGAG GACCAGCACC CCAGGGCCGG GGCCGTTCTC
  661 GGCCTTGTGT CCTGAGTGTC TGTCGGGCAG TGGCAGGGAG GACGAGGANG CCGANGGGTC
  721 CAGGGGGACG CTCTCCATGT TCTCTGGCTC CAGGTCCAGC TCCTCCGGCT CGGGCAGCTT
  781 GTTCTCCTCG CTGTAGTAGA ANGAGACCTC CCGGAAGCCA GGCTCCATCT CCTCTTTGAT
  841 GCTGCTGANG NTCTCCAGGA ANGAANGCCT CATCTTGGGG TTATACTGNC AGCACATGCG
  901 CATCANTTCA AACAGCATGT CNNNANAGTT GNCTGGNTTG TCCAGAANNN NGCCCTNNNT
  961 GNNNAANCNN ANNNNNNGCN CNNNGGNNNN GCCCNNNNNN GNCNNNNNNG NNNNNNNNNG
1021 NNNNNNNNCN NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB07648_B2Dsc.seq check
>07648_07648_B2Ds_1_SV40pro_ori_F_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNGCC TCTGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGNCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
  181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
  241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
  301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
  361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
  421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
  481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
  541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
  601 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
  661 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG
  721 CCTGCTTGCC GAATATCATG GTGGAAAATG NCCGCTTTTC TGGATTCATC GACTGTGGCC
  781 GGCTGGGTGT GNCGGANCGC TATCANGANA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG
  841 ANCTTGGCGG CGAATGGGCT GANCGCTTCC TCGNGCTTTA CNGTATCGCC GCTNCCGANN
  901 CGCAGCGNAT CGCNNTCTAT CGCNNTCNNG ACGANNNNNN CNGANCNGNN NTNNGGGGNT
961 NNNNAATGNN NNANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.