Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1(-)-h Epidermal growth factor receptor (#RDB07600)

Comment cDNA from BC094761.
Vector backbone pcDNA3.1(-) (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human epidermal growth factor receptor cDNA
Depositor|Developer Hamada, Hirofumi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07600 pcDNA3.1(-)-h Epidermal growth factor receptor DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(-)-h Epidermal growth factor receptor was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07600).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07600_B1Fgp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'
Sequence file: RDB07600_B1Fga.seq check
>07600_07600_B1Fg_3_CMV-Forward_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNTNN NNNCNNTGGG CGGTANGCGT GTACGGTGGG NGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC GGGCCCTCTA GACTCGAGCG
  181 GCCGCCGCCG CCCAGACCGG ACGACAGGCC ACCTCGTCGG CGTCCGCCCG AGTCCCCGCC
  241 TCGCCGCCAA CGCCACAACC ACCGCGCACG GCCCCCTGAC TCCGTCCAGT ATTGATCGGG
  301 AGAGCCGGAG CGAGCTCTTC GGGGAGCAGC GATGCGACCC TCCGGGACGG CCGGGGCAGC
  361 GCTCCTGGCG CTGCTGGCTG CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT
  421 TTGCCAAGGC ACGAGTAACA AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG
  481 CCTCCAGAGG ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT
  541 GCAGAGGAAN TATGATCTTT CCTTCTTAAA GACCATCCNG GANGTGGCTG GNTATGTCCT
  601 CNTTGCCCTC NACACAGTGG AGCGAATTCC NTTGGAAAAC CTGCAGATCA TCAGAGGAAA
  661 TNNGTACTAC NAAANNNNTA TGNCNTANCN NTCNNATCNN NNTATGANGC NNNAAAANNN
721 GANNGNANNN NNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHp A,insert 3'
Sequence file: RDB07600_B1Fgb.seq check
>07600_07600_B1Fg_3_bGB_rev3_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGGGCNN ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGTT TAAACTTAAG CTTGGTACCG GGCCCCCCCT CGAAGGATCA
  121 GGCCCTTATG GCCGGATCCA ACGGTGGAGA GTTTTTTTTT TTTTTTTCTG GATAAAGCTT
  181 AAAATTTATT AATATTAACT AATATTTAAT TAGTTAAATA TACGTTTCAC AGGTCAAGAT
  241 AGAGCCAGCA GAGAGAACAG GGATCAAATC TGTCACAAAC ACACACATAC ACACACACAC
  301 ATACACAGAT GTACACACAT CATGAACACT CACACATACA GATGTGTATG TCCGTGATTT
  361 GCTTCATAAG CTAGAAATTT TGCAGATTCA TTCTTACAAC AGAGGTACAG CAAACAACCA
  421 GACACAACAA AGAGCTTGTG CAGGCTCGGT CATGTGTTTA TCTGCTCCTT ACGCCCTTCA
  481 CTGTGTCTGC AAATCTGCCA CTGTTTCCCC CTCTGGAGAT GCTGGAGGGA GCANCTGGCT
  541 TGGACACTGG AGACTGGCTG CTGCTGAGTG GGTCNAGGAN GTTGANGANC AGGANTGTTT
  601 CCAGACNAAG CCACTCACCA NNCACTGNNN NNAANGTGTC NTCTATGCTG TCCTCNGTNN
661 NNGCNNCTGN NNGNNCNNAG CNGNATCNNT NCNNNAANNN NTGTNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB07600_B1Fgc.seq check
>07600_07600_B1Fg_3_SV40pro_ori_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGCC TCNGCCTCTG NNCTATTCCN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
  181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
  241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
  301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
  361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
  421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
  481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
  541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
  601 GTCTTGTCGA TCAGGANGAT CTGGACGAAN AGCATCANGG GCTCGCGCNN GCCGAACTGT
  661 NNGCNNNGCT NNANNGNGCG NATGNNNNAN GGCNANGNAN CTNNNTCNNG ACCNANGGNN
721 NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.