Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-Venus-NLS

Lentivirus vector plasmid of Venus fluorescent protein with NLS driven by human EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18579_B0I9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1alpha pro,Venus 5' Sequence file: RDB18579_B0I9a.seq
>07563_18579_B0I9_2_EF1a-C_F_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNTTTTN AGTTTGGNNC TTGGTTNNTT CTCNAGCCTC AGACAGTGGT    61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAACACGC TACCGGTCTC GAGAATTCTG   121 CAGCGGCCGC GGATCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC   181 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG   241 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT   301 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG   361 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT   421 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA   481 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA   541 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAC   601 CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA   661 CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT   721 GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA   781 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCNT   841 GGANGANCTG TACAAGTCCG GANTCANATC TCGANCTGAT CCAAAAAAGA AGANAAAGGT   901 AGATCCNAAA AAGAAGANAA NGGTANATNC AAAAAANAAN ANAANGGNNN NATCCTCTAG   961 CNANAGTTNN NNTCGAGGGA TCNNGCTNAT CNATNNTNNN NCNTCNNGNA NNANNANANT 1021 NNNNNANNNN NNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,3x SV40 NLS,Venus 3' Sequence file: RDB18579_B0I9b.seq
>07563_18579_B0I9_2_PRE_rev_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNN AGCNNCNTAG TTAAGAATAC CAGTCAATCT TTCACAAATT    61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGCTAGAG   121 GATCCTACCT TTCTCTTCTT TTTTGGATCT ACCTTTCTCT TCTTTTTTGG ATCTACCTTT   181 CTCTTCTTTT TTGGATCAGC TCGAGATCTG AGTCCGGACT TGTACAGCTC GTCCATGCCG   241 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG   301 GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG   361 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG   421 ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA   481 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC   541 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG NTCACCAGGG TGTCGCCCTC NAACTTCNCC   601 TCNGCGCGGG TCTNGNANTN GCCGNCGNCC TTGNANAANA NNGNGNGCTN NNNNNNGTAN   661 NCNTNCNGGN NTNNNNNANN NGNANANNTC NNGNTGNNTN NNGNNGNNNN GGNNANNNGN 721 NNNANNNNTG NNNNNNNANN NNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR, HIV-1 psi Sequence file: RDB18579_B0I9c.seq
>07563_18579_B0I9_2_CMV_Forward_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNC NNNTGGGCGG TAGGCGTGTA CGGTGGGAGG    61 TCTATATAAG CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC   121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA   181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA   241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA   301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA   361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG   421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA   481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA   541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA   601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT   661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA   721 AGGAAGCTTT AGACNAGATA GAGGAAGAGC AAAACNAAAA GTAAGACCAC CGCACAGCAA   781 GGGCCGCTGA TCTTCAGACC TGGANGAGGA GANATGAGGG ACAATTGGAG AAGTGAATTA   841 TATNAATATA ANGNANNNAA AATNGAACCA TTANGANTAN NACCCANNNA GGNNAAGANA   901 ANANNGGNGC AGANANNAAA AANANCNNNT GGGAATNGGA NCTTTGNTCC TTGGGTTCTT   961 GGGAGCANCN NNNNCACTNN GGGGCGCNNC NNNNNNGNNN NNCNNNNNGN NCANGNCCNN  1021 ANAATTNNTN NNNNNGGNNA NNNNNGNNAN CANNNNNANN NNNNTTNNNN NNNNGGNNNN 1081 NNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS,RRE Sequence file: RDB18579_B0I9d.seq
>07563_18579_B0I9_2_cPPT+CTS-E_R_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA GTTTGNATGT CTGTTGCTAT TATGTCTACT    61 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT   121 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC   181 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA   241 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT   301 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT   361 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC   421 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT   481 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT   541 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC   601 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG   661 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT   721 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG   781 CGTCATTGAC GCTGCGCCCA TAGTGCTTCC TGCTGCTCCC AAGAACCNNA GGAACAAAGC   841 TCCTATTCCC ACTGCTCTTT TTTCTCTCTG CNCCACTCTT CTCTTTGCCN TGGTGGGNGC   901 TACTCCTAAN GGTTCNATTT TTACNACTTT ATATTTATAT AATTCACTTC NNNNATTGTC 961 CCNCATATCT NCNNCNCCAN GNCTGANNAT CANCNGNNCC TTGCTGNGNN NNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGHpA,3' LTR, PRE Sequence file: RDB18579_B0I9e.seq
>07563_18579_B0I9_2_bGH_rev3_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNGNNNNN NGGNNGGGCN NACNANNGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG   301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT   361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT   421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT   481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT   541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC   601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG   661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC   721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC   781 GACGGTATCG ATGCGGGGAG GCGGNCCAAA GGGAGATCCG ACTCGTCTGA NGGCGAANGC   841 GAAGANGCGG AAGANGNCGC ANANCCGGCA GCANGCCGCN GGAAGGAANG TCCGCTGGAT   901 TGANGGCCNA ANGGANNTAN NANAANNNAN GTCCCGCGCA NAATCCNNGN GGNNNNNCAG 961 GNNANCANNN NTNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo,SV40pA Sequence file: RDB18579_B0I9f.seq
>07563_18579_B0I9_2_SV40pro_ori_F_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCTCTGCCT CTGAGCTATT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA   181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG   241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT   301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA   361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT   421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT   481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA   541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC   601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT   661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC   721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC   781 ACTGCATTCT AGTTGTGGTT TGTCCNAACT CATCAATGTA TCTTATCATG TCTGTATACC   841 GTCNANCTCT ANCTAGAGCT TGNCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG   901 TTATCCGCTC NCAATTNCNC ACAACATACN AGCCNGANNC ATAAAGTGTA AAGCCNGGGG   961 NGCNTNNTGA GNGANCTANC TCNNNTTANN NNCNNNGCGC NNNCNGNCNN NNTNNCNNNN 1021 NGGNNAANCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

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