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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phMET

Promoter collection, Human MET promoter

Catalog number RDB07554
Resource name pGL4-phMET
Alternative name U2872-10
Clone info. PCR amplified human MET promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 116670988 to 116672444(NC_000007.14); relatively -1208 to +249, where +1 corresponds to 1 nt of NM_001127500.3.
Comment PCR cloning, forward primer: CCTGCAAAATGGTTCAATGCAA; reverse primer: CTGCTCACAAAGCGCTCGG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human met proto-oncogene (hepatocyte growth factor receptor) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human met proto-oncogene (hepatocyte growth factor receptor)

          Reference sequence
            

          Distribution information

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          RDB07554 pGL4-phMET DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07554.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07554_A3Hc.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB07554_A3Hca.seq check
          >07554_07554_A3Hc_pGL4-4174F_G03_21.ab1
              1 GCTTGGGGCA GAAATTTCTC TGGCCTACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
             61 TCCTGCAAAA TGGTTCAATG CAAGACTTTA GTAACGTAAT GGGAACTTTC CTTTTCCATA
            121 AAACTGGGGA ATCAAGAGGT AATCTCTTTT GAGGACTGAA ATCACTCTTA TGTAACCTCT
            181 GGTTACATTA TCATTTCCAA GTGCCTGGCA CTTGGGAAAT GATAACTATT CTTACTACAT
            241 TTTTCTATGT TTCATTCTGT AGTAAATAAG AACTGAACCT GCATAGTAAC TGTTATTTTA
            301 ACCCATGACT TTCAATAACG AAGATATCTA TGTCTCATTA TCTATTGCCA TGATTGAACA
            361 AGTTGGTATG AGAGCCGGAA CGAACTCAAG TTCTAACCGG CAATGCCCGT TCCTTAGATC
            421 CTATTACCTT TGAGTGTTCA TTTACTCTTG TAGGTGCCAA TTTTTATAGC GAAATACAAA
            481 GTTATCCCAA CACAATTACT CCTAATAGAG TTCACCGAGG CCCCAAAAGC TCTTTTTTTA
            541 AAATCATCAT AAGATTTCAA CATTCAAGAA TTAAACTTTT GTTCTGTTGT GCTTATTCAT
            601 CGCTATTTGC CCAGTTATTT AATCAGCCTG CTTCCGGCTA TGGAAAAAAA AAAAAAGAAA
            661 AAAAGAAATG GAAGTCTCCT CAGGGTTAAA CTCCTCTGTT GTTCTTCCTT GCAGAAATTT
            721 GAGTTATTAT AGTAGAGGAT AATCGTTGCA TAATGAAATC ATTGGGACAA TTCGTCCATC
            781 CACTTCTACC TCCGCCTCTA ACAATGAACT CCTTGTTTCT GCGGTGCCCA AATCTCTCTA
            841 AACCCGGGTG GGCGCGGGGC GGTTAGCGGA GACGTGGGAG AGGCCGAGAG CAAAGCTCGC
            901 GCCCTTCCCG GGGTCAGCGA GCGGGGGTGC CAGGAGGGTG CGCGCCCTGC CTCTGAGCCC
            961 GGGGTGACAC TCGCCTCCAA GCGCCAGGAG GGGGAGACTC GGGTCCCGCT TATCTCCGGC
           1021 TGTGCTAACT TCAGACTGCC TGAGCTGGGG GGAGGGAGAG CCGCGGCAGC CCAGGGCGAG
           1081 AAAAACTTTC TCCAACTTAG AAGTTCACTT GCTCGTGGGC GGGGCAGAAA GGTCGGGGAA
          1141 GGTAA
          //
          Primer:
          Sequence file: RDB07554_A3Hcb.seq check
          >07554_07554_A3Hc_pGL4-136R_H03_24.ab1
              1 GCGCTTTGGG TTTTTGGGAT CTTCCATGGT GGCTTTACGA ACAGTACCGG GATTGCCAAG
             61 CTTGGCCGCC GAGGCCAGAT CTTGATCTGC TCACAAAGCG CTCGGGGCGC CGCGGGCGGC
            121 GAGGGCCTCC GGGTCACCTG CGGCCGGGCT GAGCCCAGCG CCCCGCCCGC CCGCCGGCCG
            181 CACTCCCTCC GCCCCTTCCC ACACGCGCGC TGCCCTGCCA GTGACTCAGC CGGGCATCGG
            241 CGCGCGCGGC CCGCGGCGGC CCGGGCGCGT TGGGGCCGGA GGCACCGCCA GGCGACCAGA
            301 CTGAGGCGCT CGCCTGCCCG CCCCAGCACG TGTCTGTCTG CCTCGCGTGC TGTCCGTCCC
            361 CTAGTCCGCG GGTTCCGAGG ACCGCTGAGG TGGGGACTGG CACCACCCAG AGGGAAATCG
            421 GCCCCCGCCC TTCCTGCCGT CCGCGCCGCG CCTGGCCCCG CGGGGGTCGC GTTTCCTCCC
            481 GCCTCTGCCC CGCCCACGAC AAGGTGAAAC TTTCTAGGTG GAGAAGTTTT CTCGCCCTGG
            541 CTGCGCGCTC TCCTCCCCCA GCTCAGGCAG TCTGAAGTTA GCACAGCCGG AGATAAGCGG
            601 GACCGAGTCT CCCCCTCCTG GCGCTTGGGA GGCGAGTGTC ACCCCGGGCT CAGAGGCAGG
            661 GCGCGCACCC TCCTGGCACC CCGCTCGCTG ACCCCGGGAA GGGCGCGAGC TTTGCTCTCG
            721 GCCTCTCCCA CGTCTCCGCT AACCGCCCCG CGCCCACCCG GGTTTAGAGA GATTTGGGCA
            781 CCGCAGAAAC AAGGAGTTCA TTGTTAGAGG CGGAGGTAGA AAGTGGATGG ACGAATTGTC
            841 CCAATGATTT CATTATGCAA ACGATTATCC TCTACTATAA TAACTCAAAT TTCTGCAAGG
            901 AAGAACAAAC AGAGGAGTTT AACCCCTGAG GAGACTTCCA TTTTCTTTTT TTTTCTTTTT
            961 TTTTTTTTTT TTCCCATAGC CGGGAAGCAG GGCTTGAATT AAAATAAACT TGGGGCAAAA
           1021 TAGCCGAATG AATAAAGCAA CCACACAAGA AACAAAAAAG GTTTTAAATT TCTCTTTGAA
           1081 ATGGTTGGAA AATCCTTTAT AGGAATGATT TTTTAAAAAA AGAGAAGCCT TTTTTGGGGG
           1141 TCTTCCGGTG GAAACCTCTA ATTAAGGGAG ACTAAAATTT GGGGTGGTTT GGGGGAGAAT
          1201 ACACACTCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl