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pGL4-phMET (#RDB07554)

Promoter collection, Human MET promoter

Alternative name U2872-10
Clone info. PCR amplified human MET promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 116670988 to 116672444(NC_000007.14); relatively -1208 to +249, where +1 corresponds to 1 nt of NM_001127500.3.
Comment PCR cloning, forward primer: CCTGCAAAATGGTTCAATGCAA; reverse primer: CTGCTCACAAAGCGCTCGG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human met proto-oncogene (hepatocyte growth factor receptor) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07554.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07554 pGL4-phMET DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMET was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07554).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07554_A3Hc.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB07554_A3Hca.seq check
>07554_07554_A3Hc_pGL4-4174F_G03_21.ab1
    1 GCTTGGGGCA GAAATTTCTC TGGCCTACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
   61 TCCTGCAAAA TGGTTCAATG CAAGACTTTA GTAACGTAAT GGGAACTTTC CTTTTCCATA
  121 AAACTGGGGA ATCAAGAGGT AATCTCTTTT GAGGACTGAA ATCACTCTTA TGTAACCTCT
  181 GGTTACATTA TCATTTCCAA GTGCCTGGCA CTTGGGAAAT GATAACTATT CTTACTACAT
  241 TTTTCTATGT TTCATTCTGT AGTAAATAAG AACTGAACCT GCATAGTAAC TGTTATTTTA
  301 ACCCATGACT TTCAATAACG AAGATATCTA TGTCTCATTA TCTATTGCCA TGATTGAACA
  361 AGTTGGTATG AGAGCCGGAA CGAACTCAAG TTCTAACCGG CAATGCCCGT TCCTTAGATC
  421 CTATTACCTT TGAGTGTTCA TTTACTCTTG TAGGTGCCAA TTTTTATAGC GAAATACAAA
  481 GTTATCCCAA CACAATTACT CCTAATAGAG TTCACCGAGG CCCCAAAAGC TCTTTTTTTA
  541 AAATCATCAT AAGATTTCAA CATTCAAGAA TTAAACTTTT GTTCTGTTGT GCTTATTCAT
  601 CGCTATTTGC CCAGTTATTT AATCAGCCTG CTTCCGGCTA TGGAAAAAAA AAAAAAGAAA
  661 AAAAGAAATG GAAGTCTCCT CAGGGTTAAA CTCCTCTGTT GTTCTTCCTT GCAGAAATTT
  721 GAGTTATTAT AGTAGAGGAT AATCGTTGCA TAATGAAATC ATTGGGACAA TTCGTCCATC
  781 CACTTCTACC TCCGCCTCTA ACAATGAACT CCTTGTTTCT GCGGTGCCCA AATCTCTCTA
  841 AACCCGGGTG GGCGCGGGGC GGTTAGCGGA GACGTGGGAG AGGCCGAGAG CAAAGCTCGC
  901 GCCCTTCCCG GGGTCAGCGA GCGGGGGTGC CAGGAGGGTG CGCGCCCTGC CTCTGAGCCC
  961 GGGGTGACAC TCGCCTCCAA GCGCCAGGAG GGGGAGACTC GGGTCCCGCT TATCTCCGGC
 1021 TGTGCTAACT TCAGACTGCC TGAGCTGGGG GGAGGGAGAG CCGCGGCAGC CCAGGGCGAG
 1081 AAAAACTTTC TCCAACTTAG AAGTTCACTT GCTCGTGGGC GGGGCAGAAA GGTCGGGGAA
1141 GGTAA
//
Primer:
Sequence file: RDB07554_A3Hcb.seq check
>07554_07554_A3Hc_pGL4-136R_H03_24.ab1
    1 GCGCTTTGGG TTTTTGGGAT CTTCCATGGT GGCTTTACGA ACAGTACCGG GATTGCCAAG
   61 CTTGGCCGCC GAGGCCAGAT CTTGATCTGC TCACAAAGCG CTCGGGGCGC CGCGGGCGGC
  121 GAGGGCCTCC GGGTCACCTG CGGCCGGGCT GAGCCCAGCG CCCCGCCCGC CCGCCGGCCG
  181 CACTCCCTCC GCCCCTTCCC ACACGCGCGC TGCCCTGCCA GTGACTCAGC CGGGCATCGG
  241 CGCGCGCGGC CCGCGGCGGC CCGGGCGCGT TGGGGCCGGA GGCACCGCCA GGCGACCAGA
  301 CTGAGGCGCT CGCCTGCCCG CCCCAGCACG TGTCTGTCTG CCTCGCGTGC TGTCCGTCCC
  361 CTAGTCCGCG GGTTCCGAGG ACCGCTGAGG TGGGGACTGG CACCACCCAG AGGGAAATCG
  421 GCCCCCGCCC TTCCTGCCGT CCGCGCCGCG CCTGGCCCCG CGGGGGTCGC GTTTCCTCCC
  481 GCCTCTGCCC CGCCCACGAC AAGGTGAAAC TTTCTAGGTG GAGAAGTTTT CTCGCCCTGG
  541 CTGCGCGCTC TCCTCCCCCA GCTCAGGCAG TCTGAAGTTA GCACAGCCGG AGATAAGCGG
  601 GACCGAGTCT CCCCCTCCTG GCGCTTGGGA GGCGAGTGTC ACCCCGGGCT CAGAGGCAGG
  661 GCGCGCACCC TCCTGGCACC CCGCTCGCTG ACCCCGGGAA GGGCGCGAGC TTTGCTCTCG
  721 GCCTCTCCCA CGTCTCCGCT AACCGCCCCG CGCCCACCCG GGTTTAGAGA GATTTGGGCA
  781 CCGCAGAAAC AAGGAGTTCA TTGTTAGAGG CGGAGGTAGA AAGTGGATGG ACGAATTGTC
  841 CCAATGATTT CATTATGCAA ACGATTATCC TCTACTATAA TAACTCAAAT TTCTGCAAGG
  901 AAGAACAAAC AGAGGAGTTT AACCCCTGAG GAGACTTCCA TTTTCTTTTT TTTTCTTTTT
  961 TTTTTTTTTT TTCCCATAGC CGGGAAGCAG GGCTTGAATT AAAATAAACT TGGGGCAAAA
 1021 TAGCCGAATG AATAAAGCAA CCACACAAGA AACAAAAAAG GTTTTAAATT TCTCTTTGAA
 1081 ATGGTTGGAA AATCCTTTAT AGGAATGATT TTTTAAAAAA AGAGAAGCCT TTTTTGGGGG
 1141 TCTTCCGGTG GAAACCTCTA ATTAAGGGAG ACTAAAATTT GGGGTGGTTT GGGGGAGAAT
1201 ACACACTCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles