Resource data sheet
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CSII-CMV-Venus-NLS

Lentivirus vector construct of Venus fluorescent protein with NLS driven by CMV promoter.

Catalog number RDB07550
Resource name CSII-CMV-Venus-NLS
Clone info. Lentivirus vector construct of Venus fluorescent protein with NLS driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15718_A7Lmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' RDB15718_A7Lma.seq
>07550_15718_A7Lm_CSII-CMV5'_C01_03_ABI08.ab1
    1 GTTTTCCGTG GCCTTCGGGT CTCGAGATTC TGCAGCGGCC GCGGATCCAC CATGGTGAGC
   61 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  121 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  181 ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  241 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
  301 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  361 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  421 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  481 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
  541 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC
  601 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  661 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  721 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
  781 TCTCGAGCTG ATCCAAAAAA GAAGAGAAAG GTAGATCCAA AAAAAGAAGA GAAAGGTAGA
  841 TCCAAAAAAG AAGAGAAAGG TAGGATCTCT CTAGCTAGAG TTAACATCGA GGGATCAAGC
  901 TTTATCGATA ATCAACCTCT GGGATTACAA AATTTTGTGA AAGATTGACT GGTATTTCTT
  961 TAACTATGGT TGCTTCCCTT TTAACGCTAT GGTGGGATAC GCTGCTTTTA TGCCTTTTGT
 1021 AATCATGCTA ATTGCTTTCC GTATGGCTTT TCAATTTTCT CCTTCGTTGT AATAAATTCC
1081 CTGTATGGCT GGTTCTTCAT TTAATTGAGG CAGATTGGTG TGGACTACT
//
Primer: PRE_rev RDB15718_A7Lmb.seq
>07550_15718_A7Lm_PRE_rev_D01_04_ABI08.ab1
    1 AGGTTTCAAA TAGCGTAAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGCTAGA
  121 GGATCCTACC TTTCTCTTCT TTTTTGGATC TACCTTTCTC TTCTTTTTTG GATCTACCTT
  181 TCTCTTCTTT TTTGGATCAG CTCGAGATCT GAGTCCGGAC TTGTACAGCT CGTCCATGCC
  241 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT
  301 GGGGTCTTTG CTCAGGGCGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG
  361 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC
  421 GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT
  481 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC
  541 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC
  601 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA
  661 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC
  721 GAAGCACTGC AGGCCGTAGC CCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT
  781 GCCGGTGGTG CAGATCAGCT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC
  841 GCCGGACACG CTGAAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGGC
  901 ACCACCCCCG GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGGTGGATCC GCGGCCCGCT
  961 GCAGAATTTC TCGAGACCGG TTAGCGCCTA GCGGATCTGA CGGTTCACTA AACCAGGCTC
 1021 TGGCTTATAT AGACCTCCCC ACCGTTACAC GGCCCTACCC GCCCCATTTG GCGTCAATGG
1081 AGCTGTAGTT GGTACGACAA TTGAAGCTC
//
Primer: HIVgp_R1 RDB15718_A7Lmc.seq
>07550_15718_A7Lm_HIVgp_R1_E01_05_ABI08.ab1
    1 CAGGCATTCT ATTCCCACTG CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT
   61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA
  121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG
  181 GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT
  241 ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT
  301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT
  361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT
  421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC
  481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT
  541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC
  601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG
  661 TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC
  721 TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC
  781 TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT
  841 GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC CTCCCACCGT
  901 ACACGCCTAC CGCCCATTTG CGTCAATGGG GGCGGAGTTG TTACGACATT TTGGAAAGTC
  961 CCGTTGATTT TGGGTGCCAA AACAAAACTT CCCAATTGAC GGTCCATGGG GTTGCAGGAC
 1021 CTGGAAATCC CTCGTGAGGT CAAACCGCTA TCCAACGCCC CATGATGTAC CTGCTGAAAT
1081 GCATTCACCA TGGTAATAGC CGAATGGAAC TA
//
Primer: BGH_rev2 RDB15718_A7Lmd.seq
>07550_15718_A7Lm_BGH_rev2_F01_06_ABI08.ab1
    1 CAGGAAGGGT TAGGAAGGCA CGGGGGGAGG GGCAACAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA
  841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAGGA AGGTCCGCTG
  901 GATTGAGGGC CCGAAGGGAC GTAGCAGAAG GGACGTCCCG CGCAGAATCC AGGTGGCAAC
  961 ACAGGGCGAG CAGCCATGGG AAAGGGACGT CAGCTTTCCC CGACACACAC GGGAGTTGTC
 1021 AGTGCCCACC AGTGAGCCCC TGTCAGCAGC CGGGCCAGGC AGCGCGAATG AGTCCGTCGT
1081 GCAATAGGAA GGGGGAAGGC GAAAGTTC
//
Primer: SV40pro_F RDB15718_A7Lme.seq
>07550_15718_A7Lm_SV40pro_F_G01_07_ABI08.ab1
    1 CACTTGGTCC TTGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
  241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
  301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
  361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
  421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
  481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
  541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
  661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
  721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
  781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC
  841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAAATGAGTG AGCTAACTCA
  961 CATTAAATTT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGGAAACCTG TCGTGCCAGC
 1021 TGCATTTAAT GAATCGGCCA ACGCCGCGGG AGGAGGCGGA TTTGCCGTAA TGAGGCGCCT
1081 CATGCGCCGT TCCCTTTCGG CATCCAG
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References


2018.03.15

GNP_filter3_RDBDEP_html_171214.pl