RDB07541 - pGL4-phCYP1A1

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pGL4-phCYP1A1 (#RDB07541)

Promoter collection, Human CYP1A1 promoter

Alternative name	U2842-3
Clone info.	PCR amplified human CYP1A1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 74726956 to 74725495(NC_000015.10); relatively -1428 to +34, where +1 corresponds to 1 nt of NM_000499.5.
Comment	PCR cloning, forward primer: GGATGGCGCGTAACGATGT; reverse primer: GGATCAGGGAAGGTTCCAAGG.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human cytochrome P450, family 1, subfamily A, polypeptide 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07541.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07541	pGL4-phCYP1A1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCYP1A1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07541).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15081_A7Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB15081_A7Eta.seq
>RDB07541_15081_A7Et_pGL4-4174F_F02_17_ABI24.ab1 1 ATAGGGCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT 61 GGATGGCGCG TAACGATGTT AGCTGGGGCC AGGTTGAGCT AGGCACGCAA ATACAACTTT 121 TTTTTTCCTG GAAACCCTGT AACAGGAAGG TTCCGGAGGG CGGGACAGCG TCGGAGGCAG 181 GCAGCTAGGC CATGCCAAAT GGCACTGGGG CTTCGTGTCG TGCCACAGGC GTGGACCGAA 241 AATGCGGACA CATGCAGGCT GCCTCTCCTC GCAGGCAGAA GCCACACGCA GACCTAGACC 301 CTTTGCACCG CATCCCCTTA TTCAATCGCG CACCCGCCAC CCTTCGACAG TTCCTCTCCC 361 TCCACCCCAA CCCCACGCCG CGCGCGAGGC TGGCCCTTTA AGAGCCCCGC CCCGACTCCC 421 TCCCCCCTCG CGTGACTGCG AGCCCCCGCG CCGGGCCGGG GAATGGGTCG GCTGGGTGGC 481 TGCGCGGGCC TCCGGTCCTT CTCACGCAAC GCCTGGGCAC CGCGCCTCCG GGCCAGGTGG 541 GGCGGGGACG GGCCGCCTGA CCTCTGCCCC CTAGAGGGAT GTCGCCGGCG CACGCAAGCT 601 AGCCGGGGGT AGGGTGGGGG CTCCGCGCCA GGTGCCCCCT CCGTGGTCCC TGGGCCCGAG 661 TCTTTCCGTG GCCCCCCGCC GCCGGATTTC TGTGCTCTGC CAATCAAAGC ACTAGCCACC 721 CCGGGAGCCA AGAGGGACCC TCAAGGGCCG GTGGGTCCTG GCTGGAGGGA CCGCGCGTTG 781 CAATCAGCAC TAAGGCGATC CTAGAGGCTG CGAGGAGCCG CTAGTGAGCG CTCAGCGAGC 841 CTGCCCCTTC GCCATCCATT CCGATCCTTC AATCAAGAGG CGCGAACCTC AGCTAGTCGC 901 CCGGGCTCTG GGGGACAGGT CCAGCCCCGC GGCGCCTCTG GCCTTCCGGC CCCCGTGACC 961 TCAGGGCTGG GGTCGCAGCG CTTCTCACGC GAGCCGGGAC TCAGTAACCC CGGGAAGGAG 1021 GTCACCACGG GGCAGCCCCG CCCCCGCCTG CCGAGTCCTC TAGCTGTAGC GCTGGGGAGC 1081 CATCTGCACG CTCAAGCGAT CAGTTGGGTT GCAAAATGAC GAAGAGAGTC CCGCGCCCAG 1141 CATGAGCATC CCGTTAAC //
Primer: pGL4-136R Sequence file: RDB15081_A7Etb.seq
>RDB07541_15081_A7Et_pGL4-136R_G02_20_ABI24.ab1 1 GGTTGAGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG 61 CCGCCGAGGC CAGATCTTGA TGGATCAGGG AAGGTTCCAA GGAACTGTCA CCTTCAGGGT 121 GAGGGTGAAG GCACTGCCAC CTTTATAGGC GGGCTTGTAC GTGTGGCCAC GCCTCCTTCC 181 CTGGAGCGAG CTGAGATGGA GGGAGAAGCA GCCTGAACCG GGCTGGTCTC TCTGGGATTG 241 GAGAGAAAGG TGGCGGAGGG CGGCGGGGGT GGGGGGTCGG GTGGGCAAGA ACCCACTATG 301 GGACACATCT CGGTGCCTGT ACATAGAGGG GTGAGGGCTG GGAACACCTG GAAGTCCCAA 361 TTCCAAGGCG TCCCAAGGGC AGTGCAGAAC CCAGCCGAGG AGGGGGCTTG AGGGAGAAGT 421 CCCAGGACAG CCCGAAGAGA GGGTACGGGA AGCTCCATCC TGGGGCGCGG GGACTCCTCT 481 TCGTCATTTT TGCACCCACT GGAACGCTGG GCGTGCAGAT GCCTCCCCAG CGCTACAGCC 541 TACCAGGACT CGGCAGGCGG GGGCGGGGCT GCCCCGTGGT GACCTCCTTC CCGGGGTTAC 601 TGAGTCCCGG CTCGCGTGAG AAGCGCTGCG ACCCCAGCCC TGAGGTCACG GGGGCCGGAA 661 GGCCAGAGGC GCCGCGGGGC TGGACCTGTC CCCCAGAGCC CGGGCGACTA GCTGAGGTTC 721 GCGCCTCTTG ATTGAAGGAT CGGAATGGAT GGCGAAGGGG CAGGCTCGCT GAGCGCTCAC 781 TAGCGGCTCC TCGCAGCCTC TAGGATCGCC TTAGTGCTGA TTGCAACGCG CGGTCCCTCC 841 AGCCAGGACC CACCGGCCCT TGAGGGTCCC TCTTGGCTCC CGGGGTGGCT AGTGCTTTGA 901 TTGGCAGAGC ACAGAAATCC GGCGGCGGGG GGGCCACGGA AAGACTCGGG CCCAGGGACC 961 ACGGAGGGGG GCACCTGGCG CGGAGCCCCC ACCCTACCCC CCGGCTAGCT TTGCGTGCGC 1021 CCGGCGACAT CCCTCTAGGG GCAGAGTCAG CGGCCCGTCC CCCGCCCCAC TGACCGGGAG 1081 GCGCGATGCC AGGCCGATGC GTGAGAGGAC CGAGGCCCCG CGCCAGCCAC CCCAGGCTCG 1141 GAA //
Primer: pAxCALNL_F1 Sequence file: RDB15081_A7Etc.seq
>RDB07541_15081_A7Et_pAxCALNL_F1_H02_23_ABI24.ab1 1 ACAAGTGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA 61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC 121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT 181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT 241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC 301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG 361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG 421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG 481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA 541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC 601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT 661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA 721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC 781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG 841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC 901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGG 961 TGAACATGCC GAAGCCGTGG TGAAATGGCA CCACGCTGAG GATAGCGGTG TCGGGGATGA 1021 TCTGGGTTGC CGAAGATGGG GTCGCGGGCA TGACTGAATC GGACACAGCG ATGCGGTGCG 1081 GTAGGCTACG CCTGGCCATT CAGTACTGCC ACTACTGATC ATGATCAGGA CCGAATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Other resources in our bank

human cytochrome P450, family 1, subfamily A, polypeptide 1

External Database
Human cytochrome P450, family 1, subfamily A, polypeptide 1

Reference sequence