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pGL4-phCYP1A1

Promoter collection, Human CYP1A1 promoter

Catalog number RDB07541
Resource name pGL4-phCYP1A1
Alternative name U2842-3
Clone info. PCR amplified human CYP1A1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 74726956 to 74725495(NC_000015.10); relatively -1428 to +34, where +1 corresponds to 1 nt of NM_000499.5.
Comment PCR cloning, forward primer: GGATGGCGCGTAACGATGT; reverse primer: GGATCAGGGAAGGTTCCAAGG.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human cytochrome P450, family 1, subfamily A, polypeptide 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human cytochrome P450, family 1, subfamily A, polypeptide 1

          Reference sequence
            

          Distribution information

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          RDB07541 pGL4-phCYP1A1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07541.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15081_A7Etp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB15081_A7Eta.seq check
          >RDB07541_15081_A7Et_pGL4-4174F_F02_17_ABI24.ab1
              1 ATAGGGCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 GGATGGCGCG TAACGATGTT AGCTGGGGCC AGGTTGAGCT AGGCACGCAA ATACAACTTT
            121 TTTTTTCCTG GAAACCCTGT AACAGGAAGG TTCCGGAGGG CGGGACAGCG TCGGAGGCAG
            181 GCAGCTAGGC CATGCCAAAT GGCACTGGGG CTTCGTGTCG TGCCACAGGC GTGGACCGAA
            241 AATGCGGACA CATGCAGGCT GCCTCTCCTC GCAGGCAGAA GCCACACGCA GACCTAGACC
            301 CTTTGCACCG CATCCCCTTA TTCAATCGCG CACCCGCCAC CCTTCGACAG TTCCTCTCCC
            361 TCCACCCCAA CCCCACGCCG CGCGCGAGGC TGGCCCTTTA AGAGCCCCGC CCCGACTCCC
            421 TCCCCCCTCG CGTGACTGCG AGCCCCCGCG CCGGGCCGGG GAATGGGTCG GCTGGGTGGC
            481 TGCGCGGGCC TCCGGTCCTT CTCACGCAAC GCCTGGGCAC CGCGCCTCCG GGCCAGGTGG
            541 GGCGGGGACG GGCCGCCTGA CCTCTGCCCC CTAGAGGGAT GTCGCCGGCG CACGCAAGCT
            601 AGCCGGGGGT AGGGTGGGGG CTCCGCGCCA GGTGCCCCCT CCGTGGTCCC TGGGCCCGAG
            661 TCTTTCCGTG GCCCCCCGCC GCCGGATTTC TGTGCTCTGC CAATCAAAGC ACTAGCCACC
            721 CCGGGAGCCA AGAGGGACCC TCAAGGGCCG GTGGGTCCTG GCTGGAGGGA CCGCGCGTTG
            781 CAATCAGCAC TAAGGCGATC CTAGAGGCTG CGAGGAGCCG CTAGTGAGCG CTCAGCGAGC
            841 CTGCCCCTTC GCCATCCATT CCGATCCTTC AATCAAGAGG CGCGAACCTC AGCTAGTCGC
            901 CCGGGCTCTG GGGGACAGGT CCAGCCCCGC GGCGCCTCTG GCCTTCCGGC CCCCGTGACC
            961 TCAGGGCTGG GGTCGCAGCG CTTCTCACGC GAGCCGGGAC TCAGTAACCC CGGGAAGGAG
           1021 GTCACCACGG GGCAGCCCCG CCCCCGCCTG CCGAGTCCTC TAGCTGTAGC GCTGGGGAGC
           1081 CATCTGCACG CTCAAGCGAT CAGTTGGGTT GCAAAATGAC GAAGAGAGTC CCGCGCCCAG
          1141 CATGAGCATC CCGTTAAC
          //
          Primer: pGL4-136R
          Sequence file: RDB15081_A7Etb.seq check
          >RDB07541_15081_A7Et_pGL4-136R_G02_20_ABI24.ab1
              1 GGTTGAGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
             61 CCGCCGAGGC CAGATCTTGA TGGATCAGGG AAGGTTCCAA GGAACTGTCA CCTTCAGGGT
            121 GAGGGTGAAG GCACTGCCAC CTTTATAGGC GGGCTTGTAC GTGTGGCCAC GCCTCCTTCC
            181 CTGGAGCGAG CTGAGATGGA GGGAGAAGCA GCCTGAACCG GGCTGGTCTC TCTGGGATTG
            241 GAGAGAAAGG TGGCGGAGGG CGGCGGGGGT GGGGGGTCGG GTGGGCAAGA ACCCACTATG
            301 GGACACATCT CGGTGCCTGT ACATAGAGGG GTGAGGGCTG GGAACACCTG GAAGTCCCAA
            361 TTCCAAGGCG TCCCAAGGGC AGTGCAGAAC CCAGCCGAGG AGGGGGCTTG AGGGAGAAGT
            421 CCCAGGACAG CCCGAAGAGA GGGTACGGGA AGCTCCATCC TGGGGCGCGG GGACTCCTCT
            481 TCGTCATTTT TGCACCCACT GGAACGCTGG GCGTGCAGAT GCCTCCCCAG CGCTACAGCC
            541 TACCAGGACT CGGCAGGCGG GGGCGGGGCT GCCCCGTGGT GACCTCCTTC CCGGGGTTAC
            601 TGAGTCCCGG CTCGCGTGAG AAGCGCTGCG ACCCCAGCCC TGAGGTCACG GGGGCCGGAA
            661 GGCCAGAGGC GCCGCGGGGC TGGACCTGTC CCCCAGAGCC CGGGCGACTA GCTGAGGTTC
            721 GCGCCTCTTG ATTGAAGGAT CGGAATGGAT GGCGAAGGGG CAGGCTCGCT GAGCGCTCAC
            781 TAGCGGCTCC TCGCAGCCTC TAGGATCGCC TTAGTGCTGA TTGCAACGCG CGGTCCCTCC
            841 AGCCAGGACC CACCGGCCCT TGAGGGTCCC TCTTGGCTCC CGGGGTGGCT AGTGCTTTGA
            901 TTGGCAGAGC ACAGAAATCC GGCGGCGGGG GGGCCACGGA AAGACTCGGG CCCAGGGACC
            961 ACGGAGGGGG GCACCTGGCG CGGAGCCCCC ACCCTACCCC CCGGCTAGCT TTGCGTGCGC
           1021 CCGGCGACAT CCCTCTAGGG GCAGAGTCAG CGGCCCGTCC CCCGCCCCAC TGACCGGGAG
           1081 GCGCGATGCC AGGCCGATGC GTGAGAGGAC CGAGGCCCCG CGCCAGCCAC CCCAGGCTCG
          1141 GAA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB15081_A7Etc.seq check
          >RDB07541_15081_A7Et_pAxCALNL_F1_H02_23_ABI24.ab1
              1 ACAAGTGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
             61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
            121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
            181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
            241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
            301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
            361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
            421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
            481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
            541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
            601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
            661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
            721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
            781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
            841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
            901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGG
            961 TGAACATGCC GAAGCCGTGG TGAAATGGCA CCACGCTGAG GATAGCGGTG TCGGGGATGA
           1021 TCTGGGTTGC CGAAGATGGG GTCGCGGGCA TGACTGAATC GGACACAGCG ATGCGGTGCG
          1081 GTAGGCTACG CCTGGCCATT CAGTACTGCC ACTACTGATC ATGATCAGGA CCGAATG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl