Resource data sheet
DNA Bank Top

pGL4-phCYP1A1 (#RDB07541)

Promoter collection, Human CYP1A1 promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)
GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB07541.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name U2842-3
Clone info. PCR amplified human CYP1A1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 74726956 to 74725495(NC_000015.10); relatively -1428 to +34, where +1 corresponds to 1 nt of NM_000499.5.
Comment PCR cloning, forward primer: GGATGGCGCGTAACGATGT; reverse primer: GGATCAGGGAAGGTTCCAAGG.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Human cytochrome P450, family 1, subfamily A, polypeptide 1 genomic DNA
Depositor|Developer DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 謝辞の表明を必要とする。
付加的使用条件:
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB07541 pGL4-phCYP1A1 DNA solution


How to cite this biological resource

Materials & Methods section:

The pGL4-phCYP1A1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07541).

Reference section:

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles