RDB07534 - pGL4-phHNF4A146

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pGL4-phHNF4A146

Promoter collection, Human HNF4A promoter


Catalog number	RDB07534
Resource name	pGL4-phHNF4A146
Alternative name	U2869-7
Clone info.	PCR amplified human HNF4A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44399893 to 44401377(NC_000020.11); relatively -1.5 kb to +5, where +1 corresponds to 1 nt of NM_000457.4; relatively -1363 to +122, where +1 corresponds to 1 nt of NM_178849.2. PCR cloning, forward primer: CCTCCAACCTGCCCTTCCAG; reverse primer: CGCATTCTCCCTGCCTCCAC. Known difference : A to C substitution at　44,400,645nt of NC_000020.11.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human hepatocyte nuclear factor 4, alpha genomic DNA
Depositor\|Developer	DNA Bank, \|
Other clones in our bank	human HNF4A (NCBI Gene 3172) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 　2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 　2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB07534	pGL4-phHNF4A146	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Remarks, protocol and/or map (pdf) RDB07534.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content	Promoter Collection (Firefly Luciferase) (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

user report	Suzuki, E., Transcriptional upregulation of HNF-1β by NF-κB in ovarian clear cell carcinoma modulates susceptibility to apoptosis through alteration in bcl-2 expression. Lab. Invest. 95 (8): 962-972 (2015). PMID 26030369.

2020.02.12

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