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pGL4-phHNF4A146

Promoter collection, Human HNF4A promoter

Catalog number RDB07534
Resource name pGL4-phHNF4A146
Alternative name U2869-7
Clone info. PCR amplified human HNF4A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44399893 to 44401377(NC_000020.11); relatively -1.5 kb to +5, where +1 corresponds to 1 nt of NM_000457.4; relatively -1363 to +122, where +1 corresponds to 1 nt of NM_178849.2.
PCR cloning, forward primer: CCTCCAACCTGCCCTTCCAG; reverse primer: CGCATTCTCCCTGCCTCCAC. Known difference : A to C substitution at 44,400,645nt of NC_000020.11.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human hepatocyte nuclear factor 4, alpha genomic DNA
Depositor|Developer DNA Bank, |
 
Other clones in our bank human HNF4A (NCBI Gene 3172) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.  2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB07534 pGL4-phHNF4A146 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Remarks, protocol and/or map (pdf) RDB07534.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Promoter Collection (Firefly Luciferase) (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

user report Suzuki, E., Transcriptional upregulation of HNF-1β by NF-κB in ovarian clear cell carcinoma modulates susceptibility to apoptosis through alteration in bcl-2 expression. Lab. Invest. 95 (8): 962-972 (2015). PMID 26030369.

2020.02.12

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