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pGL4-phHNF4A146 (#RDB07534)

Promoter collection, Human HNF4A promoter

Alternative name U2869-7
Clone info. PCR amplified human HNF4A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44399893 to 44401377(NC_000020.11); relatively -1.5 kb to +5, where +1 corresponds to 1 nt of NM_000457.4; relatively -1363 to +122, where +1 corresponds to 1 nt of NM_178849.2.
Comment PCR cloning, forward primer: CCTCCAACCTGCCCTTCCAG; reverse primer: CGCATTCTCCCTGCCTCCAC. Known difference : A to C substitution at　44,400,645nt of NC_000020.11.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human hepatocyte nuclear factor 4, alpha genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07534.pdf

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RDB07534 pGL4-phHNF4A146 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHNF4A146 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07534).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07534_A8Eep1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: insert 5'
Sequence file: RDB07534_A8Eea.seq check
>07534_07534_A8Ee_pGL4-4174F_A01_01_ABI24.ab1
    1 ATTGGGTCCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATCCTCCAAC CTGCCCTTCC AGCCAGCAGT GGAGGACAGG CTCCCAGGCC TCCCAAGTCT
  121 CAGGCTTTTC TTCCTCTGCC CTCACTCTCT GCCTTCTACA TCAAGACTTT ACTTCCCCAG
  181 ATTGATTGGC AGCCCTTGAA AATGTCTGCA CAGAAGGCAA TGAGGGCTGG AGGGAGTGAG
  241 AAGAACAGAG TGCACCATGA GCTTCAGACA GCCAGGACCA GCTGTGTAAT TTTCAGGGCC
  301 CCGTCCAAAA CAAAAATGCA CAATCCCTTG TTCAAAAGTC ATGATGAATT TTAGGACAAC
  361 CACAGCAGAG CACTGAACCA GGAACCAGGA CTTTCTAAGG GTTGGGTTGC CTGTGACTGC
  421 ACTGGCGATA CCCCCACAAA GCCCACTCTG AAGGTAGGAG ACGGGTGGAG AGAAACAGGG
  481 GGATGGCAAG GGGGATACGA AACAGGGAGA GGGAGGAGGG GGAAGAGGAT GGACGTCTAC
  541 CAGGCCCCAC TTGGTGCTTG ATTTATGCCA TCTCATTTCC TTCTCAAACC ACCCTTTGAA
  601 GTTGATTGTA CATTTTACAG AAAAGGAAAC TGAGGCTCGG AGAGGAGAAT CATTTACCCA
  661 AGGTCCCAGT TAGTAGACGG TAGGTGCCTG AATGTAAATC CAGGTCTCTG CCTGCTCCGG
  721 GAGGGGGTGG GGGTGAGGGA AACAGGAGAA TGTGATGGGA AAATCCGAGA TGGAGCCAGC
  781 CTGGGCCAGA AACACTGGGA GCTGTGGGAG ACGGCGAGGG GCAGGGTGGG ATCACAGGGG
  841 AGCAGAGCGG GGAATTGGGA GGGTGAATCT GGCCCTCCCA AACTTCCAGT CCATTCTGCT
  901 CCCAGGGGGA AACCGGGAAA CTGCGGGGGA ACTGGGAAGG GGAGCTCCCA GAAACAAAGG
  961 ATTCCAGAAA GATTGGGCAT CTGGGGGCCC TGGGATTTAG GGTTTCTAAA ATCGTGGAGC
 1021 CATGGGGGCA GCCTTTATCT CTGCAAAAGC ATTGATGGTA GAAAGTCAAT GATTTGGGAA
1081 GCTAATTGGA TTAGGGGATC TTCGAGGGTA AGGGCATGTT CAGTGGCCCA
//
Primer: pGL4-136R (Pr0079)
Region: Luc 5', insert 3'
Sequence file: RDB07534_A8Eeb.seq check
>07534_07534_A8Ee_pGL4-136R_B01_04_ABI24.ab1
    1 CCCTAATGGT TTTTGGGCAT CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
   61 TTGGCCGCCG AGGCCAGATC TTGATCGCAT TCTCCCTGCC TCCACGCCGC GGCCACCTGC
  121 CCTACCCTGG GCGCCCACCC CGAAGGCCCC CGCCCTCCGC CCTCCCACTG CCTCCTCCCA
  181 GTGCCCTCTC TGCCTTCCTT TCAAACCGTC CTCTGGGAAG ATCTGCTGGG AGTCTTGGCC
  241 TAGCCTCTGT GAAGGGGTGG AGGCTCTGCC GGGGAGGGGT GGGGGTTAAT GGTTAATCGG
  301 TCCCCCGCCG GTGGATAGGC TGGGCGGGGC TGCAGGGATT TGGCTGTTTG TTGGTTTCTG
  361 GCTGACACCC GGGGTGCTAA TTACAACTGC TGGGGCCCTA ACTCACCGAT GTTCAGTTAT
  421 CAATTGTACA AGGCAGGCAT CATGACTCAC GGGCACTCAT TTGACCCTTG ACTCACCCAC
  481 CCCTCCAAGC CATTGTCACC CCAAGTCAGG CATTCTAACT GATACTATCA GGCACTGACA
  541 GCCTACCTCC GAGATCCCCT AATTCAATAA CTTCCCAAAT CATTGACTTC TACCCTCAAT
  601 GCTTTTGCAG AGATAAGGCT GCCCCATGGC CCACGATTTA GAAACCTAAA TCCCAGGCCC
  661 CAGATGCCAA TCTTCTGGAT CCTTGTTCTG GGAGCTCCCT TCCAGTTCCC CCGCAGTTTC
  721 CCGGTTCCCC TGGGAGCAGA ATGGACTGGA AGTTTGGGAG GGCCAGATTC ACCTCCAATT
  781 CCCCGCTCCT GCTCCCTGTG ATCCCACCCT GCCCCTCGCC GTCTCCCACA GCTCCCAGTG
  841 TTTCTGGCCC AGGCTGGCTC CATCTCGGAT TTTCCCATCA CATTCTCCTG TTTCCCTCAC
  901 CCCCACCCCC TCCCGGAGCA GGCAGAGACC TGGATTTACA TTCAGGCACC TACCGTCTAC
  961 TAACTGGGGA CCTTGGGTAA ATGATTCTCC TCTCCGAAGC CCTCAGTTTC CTTTTTCTGT
 1021 AAAATGTACA AATCACTTTC AAGGTGGGTT TGGAGAGGGA AATGAGATTG CATTAATCAG
1081 CACAGTGGGG CCTTGTAGAC GTCCATCCGT CATACCCCCC TTCCTGCGC
//
Primer: pAxCALNLF1 (Pr0166)
Region: Luc 3'
Sequence file: RDB07534_A8Eec.seq check
>07534_07534_A8Ee_pAxCALNL_F1_C01_07_ABI24.ab1
    1 TAAAGGAATC TTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
   61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
  121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
  181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
  241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
  301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
  361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
  421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
  481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
  541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
  601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
  661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
  721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
  781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
  841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
  901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
  961 ACATGCCGAA GCCGTGGGTG AAATGGCACC ACGCTGAGGG ATAGCGGGTG TCGGGGATGA
 1021 TCTGGTTGCC GAAGATGGGG TCGCGGGCAT GACTGATCCG GACACAGCGT GCGGTGCGGT
 1081 TAGGCCTACG CCCTTGGCAA TTCCGGTACC TGCACTACTG TTCATGATTC AGGGTCGTAA
1141 T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Suzuki, E., Transcriptional upregulation of HNF-1β by NF-κB in ovarian clear cell carcinoma modulates susceptibility to apoptosis through alteration in bcl-2 expression. Lab. Invest. 95 (8): 962-972 (2015). PMID 26030369. [link to RRC of NBRP]