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pGL4-phHNF4A146

Promoter collection, Human HNF4A promoter

Catalog number RDB07534
Resource name pGL4-phHNF4A146
Alternative name U2869-7
Clone info. PCR amplified human HNF4A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44399893 to 44401377(NC_000020.11); relatively -1.5 kb to +5, where +1 corresponds to 1 nt of NM_000457.4; relatively -1363 to +122, where +1 corresponds to 1 nt of NM_178849.2.
Comment PCR cloning, forward primer: CCTCCAACCTGCCCTTCCAG; reverse primer: CGCATTCTCCCTGCCTCCAC. Known difference : A to C substitution at 44,400,645nt of NC_000020.11.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human hepatocyte nuclear factor 4, alpha genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human hepatocyte nuclear factor 4, alpha

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07534 pGL4-phHNF4A146 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07534.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07534_A8Eep1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Region: insert 5'
          Sequence file: RDB07534_A8Eea.seq check
          >07534_07534_A8Ee_pGL4-4174F_A01_01_ABI24.ab1
              1 ATTGGGTCCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
             61 ATCCTCCAAC CTGCCCTTCC AGCCAGCAGT GGAGGACAGG CTCCCAGGCC TCCCAAGTCT
            121 CAGGCTTTTC TTCCTCTGCC CTCACTCTCT GCCTTCTACA TCAAGACTTT ACTTCCCCAG
            181 ATTGATTGGC AGCCCTTGAA AATGTCTGCA CAGAAGGCAA TGAGGGCTGG AGGGAGTGAG
            241 AAGAACAGAG TGCACCATGA GCTTCAGACA GCCAGGACCA GCTGTGTAAT TTTCAGGGCC
            301 CCGTCCAAAA CAAAAATGCA CAATCCCTTG TTCAAAAGTC ATGATGAATT TTAGGACAAC
            361 CACAGCAGAG CACTGAACCA GGAACCAGGA CTTTCTAAGG GTTGGGTTGC CTGTGACTGC
            421 ACTGGCGATA CCCCCACAAA GCCCACTCTG AAGGTAGGAG ACGGGTGGAG AGAAACAGGG
            481 GGATGGCAAG GGGGATACGA AACAGGGAGA GGGAGGAGGG GGAAGAGGAT GGACGTCTAC
            541 CAGGCCCCAC TTGGTGCTTG ATTTATGCCA TCTCATTTCC TTCTCAAACC ACCCTTTGAA
            601 GTTGATTGTA CATTTTACAG AAAAGGAAAC TGAGGCTCGG AGAGGAGAAT CATTTACCCA
            661 AGGTCCCAGT TAGTAGACGG TAGGTGCCTG AATGTAAATC CAGGTCTCTG CCTGCTCCGG
            721 GAGGGGGTGG GGGTGAGGGA AACAGGAGAA TGTGATGGGA AAATCCGAGA TGGAGCCAGC
            781 CTGGGCCAGA AACACTGGGA GCTGTGGGAG ACGGCGAGGG GCAGGGTGGG ATCACAGGGG
            841 AGCAGAGCGG GGAATTGGGA GGGTGAATCT GGCCCTCCCA AACTTCCAGT CCATTCTGCT
            901 CCCAGGGGGA AACCGGGAAA CTGCGGGGGA ACTGGGAAGG GGAGCTCCCA GAAACAAAGG
            961 ATTCCAGAAA GATTGGGCAT CTGGGGGCCC TGGGATTTAG GGTTTCTAAA ATCGTGGAGC
           1021 CATGGGGGCA GCCTTTATCT CTGCAAAAGC ATTGATGGTA GAAAGTCAAT GATTTGGGAA
          1081 GCTAATTGGA TTAGGGGATC TTCGAGGGTA AGGGCATGTT CAGTGGCCCA
          //
          Primer: pGL4-136R
          Region: Luc 5', insert 3'
          Sequence file: RDB07534_A8Eeb.seq check
          >07534_07534_A8Ee_pGL4-136R_B01_04_ABI24.ab1
              1 CCCTAATGGT TTTTGGGCAT CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
             61 TTGGCCGCCG AGGCCAGATC TTGATCGCAT TCTCCCTGCC TCCACGCCGC GGCCACCTGC
            121 CCTACCCTGG GCGCCCACCC CGAAGGCCCC CGCCCTCCGC CCTCCCACTG CCTCCTCCCA
            181 GTGCCCTCTC TGCCTTCCTT TCAAACCGTC CTCTGGGAAG ATCTGCTGGG AGTCTTGGCC
            241 TAGCCTCTGT GAAGGGGTGG AGGCTCTGCC GGGGAGGGGT GGGGGTTAAT GGTTAATCGG
            301 TCCCCCGCCG GTGGATAGGC TGGGCGGGGC TGCAGGGATT TGGCTGTTTG TTGGTTTCTG
            361 GCTGACACCC GGGGTGCTAA TTACAACTGC TGGGGCCCTA ACTCACCGAT GTTCAGTTAT
            421 CAATTGTACA AGGCAGGCAT CATGACTCAC GGGCACTCAT TTGACCCTTG ACTCACCCAC
            481 CCCTCCAAGC CATTGTCACC CCAAGTCAGG CATTCTAACT GATACTATCA GGCACTGACA
            541 GCCTACCTCC GAGATCCCCT AATTCAATAA CTTCCCAAAT CATTGACTTC TACCCTCAAT
            601 GCTTTTGCAG AGATAAGGCT GCCCCATGGC CCACGATTTA GAAACCTAAA TCCCAGGCCC
            661 CAGATGCCAA TCTTCTGGAT CCTTGTTCTG GGAGCTCCCT TCCAGTTCCC CCGCAGTTTC
            721 CCGGTTCCCC TGGGAGCAGA ATGGACTGGA AGTTTGGGAG GGCCAGATTC ACCTCCAATT
            781 CCCCGCTCCT GCTCCCTGTG ATCCCACCCT GCCCCTCGCC GTCTCCCACA GCTCCCAGTG
            841 TTTCTGGCCC AGGCTGGCTC CATCTCGGAT TTTCCCATCA CATTCTCCTG TTTCCCTCAC
            901 CCCCACCCCC TCCCGGAGCA GGCAGAGACC TGGATTTACA TTCAGGCACC TACCGTCTAC
            961 TAACTGGGGA CCTTGGGTAA ATGATTCTCC TCTCCGAAGC CCTCAGTTTC CTTTTTCTGT
           1021 AAAATGTACA AATCACTTTC AAGGTGGGTT TGGAGAGGGA AATGAGATTG CATTAATCAG
          1081 CACAGTGGGG CCTTGTAGAC GTCCATCCGT CATACCCCCC TTCCTGCGC
          //
          Primer: pAxCALNLF1
          Region: Luc 3'
          Sequence file: RDB07534_A8Eec.seq check
          >07534_07534_A8Ee_pAxCALNL_F1_C01_07_ABI24.ab1
              1 TAAAGGAATC TTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
             61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
            121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
            181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
            241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
            301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
            361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
            421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
            481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
            541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
            601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
            661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
            721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
            781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
            841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
            901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
            961 ACATGCCGAA GCCGTGGGTG AAATGGCACC ACGCTGAGGG ATAGCGGGTG TCGGGGATGA
           1021 TCTGGTTGCC GAAGATGGGG TCGCGGGCAT GACTGATCCG GACACAGCGT GCGGTGCGGT
           1081 TAGGCCTACG CCCTTGGCAA TTCCGGTACC TGCACTACTG TTCATGATTC AGGGTCGTAA
          1141 T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Suzuki, E., Transcriptional upregulation of HNF-1β by NF-κB in ovarian clear cell carcinoma modulates susceptibility to apoptosis through alteration in bcl-2 expression. Lab. Invest. 95 (8): 962-972 (2015). PMID 26030369.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl