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pGL4-phAPOB (#RDB07527)

Promoter collection, Human APOB promoter

Alternative name U2838-3
Clone info. PCR amplified human APOB promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 21045468 to 21044033(NC_000002.12); relatively -1395 to +41, where +1 corresponds to 1 nt of NM_000384.3.
Comment PCR cloning, forward primer: GCAGGCGAAGGTCTTTCTGTG; reverse primer: AACCGAGAAGGGCACTCAGC. Entire sequence of promoter region has not been confirmed. Known differences (at least): substitutions A to G at 21,044,338, T to C at 21,044,910, G to A at 21,044,941, G to T at 21,044,975, A to C at 21,045,347 of NC_000002.12.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human apolipoprotein B (including Ag(x) antigen) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07527.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07527 pGL4-phAPOB DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phAPOB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07527).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07527_A3Jmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07527_A3Jma.seq check
>07527_07527_A3Jm_pGL4-4174F_A10_01.ab1
    1 GCCCGGGGTC CAGAACATTT CCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC
   61 GAGGATGCAG GCGAAGGTCT TTCTGTGAGT CTCCTCTGAG GGAACTGGGT CTTTATACAT
  121 AGTTACTGTT TCAGAATTGA TCCTTCTGGA ATCATCAGTC TTCACCAGTA GCTTGTTACA
  181 TCTGGGGGTA TCTCATAATT CAAACAAAGC TGACAAGTTG TAACAATGAG CACACACTGA
  241 CTTCTGCAAC AGGCGCTGTC CACTTCCCAT CCGCACTCTA CCGGCTTGCT CCTGGCCGCC
  301 TCCCACTCGC CTTCCTGGGT GGTCCCCCAG CAGTTATACC TACCTGGTTG TCGCCCCCTC
  361 TATCCTACCA CAATTGCTCA CTAGCGGTTT CCTGCGTACA CAGCTTGTCT CCCTAACCAG
  421 AGTGGAGGTG CCTTGGGGAC ACAGCCAGGC TCAGACATTC ACTCAGCTCA TCATAGTGCC
  481 ATCCCATCAA TAACCCCTTC TGAGTGATCC TGGGTTAGTA AACCGAGTGT CCCTGAAATT
  541 CCACTACCGC TGATTCCCTA CAGCTGGGCA GAGGCAGCGA GCGCTGGCTG AAGTTTCCGG
  601 TGGGAAATGG GCAGTGCCTA GAAGGGAAGG AAACGATGCA TGAGAAGGTT CCAGATGTCT
  661 ATGAGGAACA TGACGTGTCC TGTCCACTAC TCTGCTTTTC CTCGTCCGCC TCCCCACCAC
  721 TGGAGGAAAC CTAGAAGCTG GTGCAGGAAA TCCTCCTCTC AACAACCCAA GAACACTTTG
  781 CACAAGAGGG GTGCGCCCTC GGAGGTTGCT CTTCCCCAGA GGCCTCTCCT CGCTGGGGTT
  841 TCTTGAAGAC AGATACTTGG ACTCCTGCTG GGACCAGGGC AGGCCACCCA TCCTCAGGGG
  901 CAGTGACTGG TCACTCACCA GACCTCCCTG CATCCCCCTT CTCTCTCCTC CCCCAGCACG
  961 GGCTGAACCC CGCAGCCACA GATTCTGATC AGGATTAGGG TGTGGGTGCA AATCCAAGGT
 1021 CCACCCAAAT GGAAAAGAAG TAACCCGATG GGGAACACGG TCTCCACCCA GACAGCGCCT
 1081 CAGGGACCTG GGTTCTCCCT CGTGGCTCCT ATTTCAGGTT CCAAGGAGAG CAGAGATTTT
1141 GTCCCAATGG TTGGGTCATC TTGAGAAAGG CCACCCCCCC TGGGTTACAG GGCCAG
//
Primer: pGL4-136R
Sequence file: RDB07527_A3Jmb.seq check
>07527_07527_A3Jm_pGL4-136R_B10_04.ab1
    1 GCCTATGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
   61 CCGCCGAGGC CAGATCTTGA TAACCGAGAA GGGCACTCAG CCCCGCAGGT CCCGGTGGGA
  121 ATGCGCGGCC GGCGCCCGCA CCCCATTTAT AGGAAGCCCA GGCTGCAAGA GCGCCAGGAT
  181 TGCAAAAGGT CCAAAGGGCG CCTCCCGGGC CTGACCTGTT TGCTTTTCTA CACTGGCTTC
  241 TCTTTGAGCC TTGAAGAGCC TCGGGGAGGG GGCCCACCTG GGATGCAGCC GCAGCCACCA
  301 GGGGCTGGGT CCCAGGTGGG TTCCCTTCCC CAAGCGTCTT CAGTGCTCTG GCGCGGCCCT
  361 TCCTGTGTCT CAGTGGGGCC ATGGCCGGCG CCTCAGGGTC TGAGAAGCCT GCCCTGACCA
  421 GGGGTGCCTT CTTCAGATGA CCCACCATGG GGACAAAATC TCTGCTTCTC CTGGACTGAA
  481 TTGGGAGCCA CGAGGAGAAC CAGTCCTGAG CGCTGTCTTG GTGGAGACGT GTTCCCATCG
  541 GTTACTTCTT TTCCATTTTG GTGGACCTTG GATTTGCACC CACACCCTAA TCCTGATCAG
  601 AATCTGTGGC TGCGGGGTTC AGCCCGTGCT GGGGGAGGAG AGAGAAGGGG GATGCAGGGA
  661 GGTCTGGTGA GTGACCAGTC ACTGCCCCTG AGGATGGGTG GCCTGCCTGG TCCCAGCAGG
  721 AGTCCAAGTA TCTGTCTTCA AGAAACCCCA GCGAGGAGAG GCCTCTGGGG AAGAGCAACC
  781 TCCGAGGGCG CACCCCTCTT GTGCAAAGTG TTCTTGGGTT GTTGAGAGGA GGATTTCCTG
  841 CACCAGCTTC TAGGTTTCCT CCAGTGGTGG GGAGGCGGAC GAGGAAAAGC AGAGTAGTGG
  901 ACAGGACACG TCATGTTCCT CATAGACATC TGGAACCTTC TCATGCATCG TTTCCTTCCC
  961 TTCTAGGCAC TGCCCATTTC CCACCGGAAA CTTCAGCCAG CGCTCGCTGC CTCTGCCCAG
 1021 CTGTAGGGAA TCAGCGGTAG TGGAATTTCA GGACACTCGG GTTTACTAAC CCCAGGATCA
 1081 CTCAGAAGGG TATGGATGGA TGCACTATGA TGAGCTGACT GAATGTCTGA GCTTGGCCTG
1141 TGTCCCCAGC ACTCACTCTG GTTAAGGGGA GAAACAA
//
Primer: pAxCALNL_F1
Sequence file: RDB07527_A3Jmc.seq check
>07527_07527_A3Jm_pAxCALNL_F1_H11_23_ABI24.ab1
    1 ATAATATATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
   61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
  121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
  181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
  241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
  301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
  361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
  421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
  481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC
  541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC
  601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC
  661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG
  721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG
  781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT
  841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC
  901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG
  961 AACATGCCGA AGCCGTGGTG AAATGGCACC ACGCTGAGGA TAGCGGTGTC GGGGATGATC
 1021 TGGTTTGCCC GAAGATGGGG GTCGCGGGCA TGACTGAATC GGACACAAGC GGGTGCGGTG
 1081 CGGTAGGCTA CGCCCTTTGG GGCATCAGTA CCTGCACTTA CCTGATCATG ATCAGGCCGA
1141 ATGGTATGGT CCGATCGAGC TCTCTCGGCA ACACGAAAGA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles