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pGL4-phAPOB

Promoter collection, Human APOB promoter

Catalog number RDB07527
Resource name pGL4-phAPOB
Alternative name U2838-3
Clone info. PCR amplified human APOB promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 21045468 to 21044033(NC_000002.12); relatively -1395 to +41, where +1 corresponds to 1 nt of NM_000384.3.
Comment PCR cloning, forward primer: GCAGGCGAAGGTCTTTCTGTG; reverse primer: AACCGAGAAGGGCACTCAGC. Entire sequence of promoter region has not been confirmed. Known differences (at least): substitutions A to G at 21,044,338, T to C at 21,044,910, G to A at 21,044,941, G to T at 21,044,975, A to C at 21,045,347 of NC_000002.12.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human apolipoprotein B (including Ag(x) antigen) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human apolipoprotein B (including Ag(x) antigen)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.  2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07527 pGL4-phAPOB DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07527.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07527_A3Jmp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07527_A3Jma.seq check
          >07527_07527_A3Jm_pGL4-4174F_A10_01.ab1
              1 GCCCGGGGTC CAGAACATTT CCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC
             61 GAGGATGCAG GCGAAGGTCT TTCTGTGAGT CTCCTCTGAG GGAACTGGGT CTTTATACAT
            121 AGTTACTGTT TCAGAATTGA TCCTTCTGGA ATCATCAGTC TTCACCAGTA GCTTGTTACA
            181 TCTGGGGGTA TCTCATAATT CAAACAAAGC TGACAAGTTG TAACAATGAG CACACACTGA
            241 CTTCTGCAAC AGGCGCTGTC CACTTCCCAT CCGCACTCTA CCGGCTTGCT CCTGGCCGCC
            301 TCCCACTCGC CTTCCTGGGT GGTCCCCCAG CAGTTATACC TACCTGGTTG TCGCCCCCTC
            361 TATCCTACCA CAATTGCTCA CTAGCGGTTT CCTGCGTACA CAGCTTGTCT CCCTAACCAG
            421 AGTGGAGGTG CCTTGGGGAC ACAGCCAGGC TCAGACATTC ACTCAGCTCA TCATAGTGCC
            481 ATCCCATCAA TAACCCCTTC TGAGTGATCC TGGGTTAGTA AACCGAGTGT CCCTGAAATT
            541 CCACTACCGC TGATTCCCTA CAGCTGGGCA GAGGCAGCGA GCGCTGGCTG AAGTTTCCGG
            601 TGGGAAATGG GCAGTGCCTA GAAGGGAAGG AAACGATGCA TGAGAAGGTT CCAGATGTCT
            661 ATGAGGAACA TGACGTGTCC TGTCCACTAC TCTGCTTTTC CTCGTCCGCC TCCCCACCAC
            721 TGGAGGAAAC CTAGAAGCTG GTGCAGGAAA TCCTCCTCTC AACAACCCAA GAACACTTTG
            781 CACAAGAGGG GTGCGCCCTC GGAGGTTGCT CTTCCCCAGA GGCCTCTCCT CGCTGGGGTT
            841 TCTTGAAGAC AGATACTTGG ACTCCTGCTG GGACCAGGGC AGGCCACCCA TCCTCAGGGG
            901 CAGTGACTGG TCACTCACCA GACCTCCCTG CATCCCCCTT CTCTCTCCTC CCCCAGCACG
            961 GGCTGAACCC CGCAGCCACA GATTCTGATC AGGATTAGGG TGTGGGTGCA AATCCAAGGT
           1021 CCACCCAAAT GGAAAAGAAG TAACCCGATG GGGAACACGG TCTCCACCCA GACAGCGCCT
           1081 CAGGGACCTG GGTTCTCCCT CGTGGCTCCT ATTTCAGGTT CCAAGGAGAG CAGAGATTTT
          1141 GTCCCAATGG TTGGGTCATC TTGAGAAAGG CCACCCCCCC TGGGTTACAG GGCCAG
          //
          Primer: pGL4-136R
          Sequence file: RDB07527_A3Jmb.seq check
          >07527_07527_A3Jm_pGL4-136R_B10_04.ab1
              1 GCCTATGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
             61 CCGCCGAGGC CAGATCTTGA TAACCGAGAA GGGCACTCAG CCCCGCAGGT CCCGGTGGGA
            121 ATGCGCGGCC GGCGCCCGCA CCCCATTTAT AGGAAGCCCA GGCTGCAAGA GCGCCAGGAT
            181 TGCAAAAGGT CCAAAGGGCG CCTCCCGGGC CTGACCTGTT TGCTTTTCTA CACTGGCTTC
            241 TCTTTGAGCC TTGAAGAGCC TCGGGGAGGG GGCCCACCTG GGATGCAGCC GCAGCCACCA
            301 GGGGCTGGGT CCCAGGTGGG TTCCCTTCCC CAAGCGTCTT CAGTGCTCTG GCGCGGCCCT
            361 TCCTGTGTCT CAGTGGGGCC ATGGCCGGCG CCTCAGGGTC TGAGAAGCCT GCCCTGACCA
            421 GGGGTGCCTT CTTCAGATGA CCCACCATGG GGACAAAATC TCTGCTTCTC CTGGACTGAA
            481 TTGGGAGCCA CGAGGAGAAC CAGTCCTGAG CGCTGTCTTG GTGGAGACGT GTTCCCATCG
            541 GTTACTTCTT TTCCATTTTG GTGGACCTTG GATTTGCACC CACACCCTAA TCCTGATCAG
            601 AATCTGTGGC TGCGGGGTTC AGCCCGTGCT GGGGGAGGAG AGAGAAGGGG GATGCAGGGA
            661 GGTCTGGTGA GTGACCAGTC ACTGCCCCTG AGGATGGGTG GCCTGCCTGG TCCCAGCAGG
            721 AGTCCAAGTA TCTGTCTTCA AGAAACCCCA GCGAGGAGAG GCCTCTGGGG AAGAGCAACC
            781 TCCGAGGGCG CACCCCTCTT GTGCAAAGTG TTCTTGGGTT GTTGAGAGGA GGATTTCCTG
            841 CACCAGCTTC TAGGTTTCCT CCAGTGGTGG GGAGGCGGAC GAGGAAAAGC AGAGTAGTGG
            901 ACAGGACACG TCATGTTCCT CATAGACATC TGGAACCTTC TCATGCATCG TTTCCTTCCC
            961 TTCTAGGCAC TGCCCATTTC CCACCGGAAA CTTCAGCCAG CGCTCGCTGC CTCTGCCCAG
           1021 CTGTAGGGAA TCAGCGGTAG TGGAATTTCA GGACACTCGG GTTTACTAAC CCCAGGATCA
           1081 CTCAGAAGGG TATGGATGGA TGCACTATGA TGAGCTGACT GAATGTCTGA GCTTGGCCTG
          1141 TGTCCCCAGC ACTCACTCTG GTTAAGGGGA GAAACAA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07527_A3Jmc.seq check
          >07527_07527_A3Jm_pAxCALNL_F1_H11_23_ABI24.ab1
              1 ATAATATATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
             61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
            121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
            181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
            241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
            301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
            361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
            421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
            481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC
            541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC
            601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC
            661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG
            721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG
            781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT
            841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC
            901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG
            961 AACATGCCGA AGCCGTGGTG AAATGGCACC ACGCTGAGGA TAGCGGTGTC GGGGATGATC
           1021 TGGTTTGCCC GAAGATGGGG GTCGCGGGCA TGACTGAATC GGACACAAGC GGGTGCGGTG
           1081 CGGTAGGCTA CGCCCTTTGG GGCATCAGTA CCTGCACTTA CCTGATCATG ATCAGGCCGA
          1141 ATGGTATGGT CCGATCGAGC TCTCTCGGCA ACACGAAAGA T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl