RDB07513 - pGL4-phMT1A

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pGL4-phMT1A (#RDB07513)

Promoter collection, Human MT1A promoter

Alternative name	U2793-9
Clone info.	PCR amplified human MT1A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 56637392 to 56638692(NC_000016.10); relatively -1274 to +27, where +1 corresponds to 1 nt of NM_005946.3.Known difference : T to C substitution at 56,637,784nt; G to A substitution at 56,637,929nt; G to C substitution at 56,638,443nt; C insertion between 56,638,444nt and 56,638,445nt.
Comment	PCR cloning, forward primer: AAAGTGGCTATAGTGCTGGAACC; reverse primer: CTATAAGGCGCACGTGGAAG. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human metallothionein 1A genomic DNA
Depositor\|Developer	DNA Bank, \|

Sequence (full)	RDB07513zzk01.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB07513.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07513	pGL4-phMT1A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMT1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07513).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07513_09J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665) Region: pause site,insert 5' Sequence file: RDB07513_09J5a.seq
>07513_07513_09J5_pGL4ins_F_A02_02_ABI24.ab1 1 NNNNNNANNN NNNNNNNNNN NNNNNAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA 61 ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TAAAGTGGCT 121 ATAGTGCTGG AACCCAACTT CTTAGTTTCT TGAATCCTAC ATGTGTGCTA TGATTTAGAT 181 ATTTTGTCAC TGCCAAATCT CATGTTGAAA TGTGTCCTCC ACTATTGCAG GTGGGGCCTA 241 GAGGGAGCTT TTGGGGTCAT GAGGACACAT CCCTAAAGAA TGACTTGGTG CCATCCCATG 301 GTAAAGAGTG TGTTCATCCT CTGGTAGGTC ACTCCAGTGG TGGCTGTGAA TGAAAGTGAT 361 TAGAAAGGGA CTGGTATAGA CTCAATGTTA TGCCTTAGTC AGGTGTACTT TAGGGAAATA 421 ACTGGATGTC TTGCTTTTTA ATTTTATTTT TTTAGAGAAG TTGACTTGCT ATGTGGACTA 481 GGCAGGACTG GAAGTCCTGG GCTCAAGTGA TGCTCCCGCC TCAGCCTCCT AAGTAGCTTG 541 GACTACAGCT TCCCGCCACC TCCCCCATCT TGCTTTTTAG TTTAAAGCAG GGTCAGCACA 601 TCACATGAAG TCATCTCCTT TTTGGGGATA TCCCACATGT CCAGAACTAC CAGACGGTAG 661 TGGGGTGGCC GGCTAGGCTG TGGGGAGCAC GGAGATTTAT TTGCAAAGGA GGACCTGGAC 721 AAATGTGCCC CCACATCCTC TCANGCGAGG AGAATGGACG AGAGTGAGAG GCCGACCCGT 781 GTTCCCGTGT TACTGTGTAC NGAGTAGTGG GTCCGAGGGA NCTANGTGTG GACAGGGACA 841 GGCAANGCGA CAGCGANGAN AAACGAAAAT CACATCNGTG GCNGTTGCTN TNCACACAAC 901 TCGCTCGCTA CCGCACGCTC CACGCTCTGC ACTACGCCNN TNCGGGNNNN NGNNCNNNAN 961 GCTNNNGCNG CNCNCNNANT TCNGNNNNNN NCNNNCNNNA ANNNNNNANN NGGNNGGGNN 1021 NNNNNNNN //
Primer: pGL4ins_R (Pr0666) Region: luc,insert 3' Sequence file: RDB07513_09J5b.seq
>07513_07513_09J5_pGL4ins_R_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGNNAGNNN GGCGCTGGGC CCTTCTTAAT GTTTTTGGCA 61 TCTTCCATGG TGGCTTTACC AACAGTACCG GATTGCCAAG CTTGGCCGCC GAGGCCAGAT 121 CTTGATCTAT AAGGCGCACG TGGAAGGCTT GGTAGGGCCC AGCAGCCAGC GGCTGCTTTT 181 ATGGTCAGGG GAAAGAGGGC CGGACGCAAA GGACCCGCCC TGCGCCTGCG CCCGCCCCGC 241 TGAGTCCGCC CAGCCGAGTC CGCCCCGCCC GAGGTGATGA CCTGGACTGG GCTTTGCGCA 301 GCAGAAAGGC AAGGCGCACA GTCCACTGAG CTCCCCTGGG TGCGGACCAC GGGACCACCC 361 CCTCCCCGCT TTGGCGCCCG CTAACGCGGG AGTGAGTTTA CGCCCGGGGT ACGGGGCGTC 421 CCCGAGGTCC CGGTCGCCTT TCCCAGTTTC CCCGAAGTTG CCTTCCACCC TAGTCCCCGC 481 GCGTTCCGGG AACCCAGCCT TGGCGTTTCG GTCGCCTCCA CCCCACCCCT CTCACGGTTT 541 TCAGCTCGGC CTGTCCCGAA GTCTGAGTGC AGCCACAGCC TCCTGCTCCT GTCCCCGGAT 601 CGGCGTAGTG CAGAGCGTGG AGCGTGCGGT AGCGAGCGAG TTGTGTGCAG AGCAACCGCC 661 ACCGATGTGA TTTTCGTTTC TCCTCGCTGT CGCCTTGCCT GTCCCTGTCC ACACCTAGGT 721 CCCTCGGACC CACTACTCCG TACACAGTAA CACGGGAACA CGGGTCGGCC TCTCACTCTC 781 GTCCATTCTC CTCGCCTGAG AGGNNGTGGG GGCACATTTG TCCAGGTCCT CCTTTGCAAA 841 TAAATCTCCG TGCTCCNCAC AGNCTANCCG GCCACCCCAC TACCGTCTGG TANTTCTGGA 901 CATGTGGNNT ATCCCCAAAA AGGANNTGAN TNCATGTGAT GNGCTGNNNN NGCTTTNAAC 961 TAAAAAGCNN NNNGGGGGNA NNTNNNNGGN ANNCTGTNNN NNNANNNNNN TNNNNNNNNN 1021 N //
Primer: pGL4pA_R (Pr0664) Region: SV40 pA,luc Sequence file: RDB07513_09J5c.seq
>07513_07513_09J5_pGL4pA_R_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCTT ANCGNNTTNA CNNCNTTTGT AGAGGTTTTA CTTGCTTTAA 61 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA 121 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 181 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 241 ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC GGCGATCTTG 301 CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC 361 AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA 421 ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC 481 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG 541 TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC TACCTGGTAG 601 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC 661 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTTGTAGCC 721 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCGCCGCGC 781 TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTANCCTC NAAGAANGGC 841 ACCACCTTGC CTACTGCGCC AGGCTTGTCG NCCCCTTCNG GGGTGATCAG AATGGCGCTG 901 GNNGTTTCTG TCANGCCGTA NCCCTGGCGG NNGNCTGGTN NNTGGAAGCG NTTGGNCACG 961 GNCTCNCCNA CCTNNNNGCT GANCNGCGNC CCGNCNNCNN GNNNTNNCNN NNNNNNTNNN 1021 NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Other resources in our bank

human metallothionein 1A

External Database
Human metallothionein 1A

Reference sequence