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pGL4-phMYB (c-myb) (#RDB07480)

Promoter collection, Human MYB promoter

Alternative name U2812-7
Clone info. PCR amplified human MYB promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 135179905 to 135181339(NC_000006.12 ); relatively -1410 to +25, where +1 corresponds to 1 nt of NM_001130173.1.
Comment PCR cloning, forward primer: TCTTGAAACCTCGCATGCTT; reverse primer: GAGGAGGAGGAAACAGGTTGA. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human v-myb myeloblastosis viral oncogene homolog genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07480.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07480 pGL4-phMYB (c-myb) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMYB (c-myb) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07480).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07480_A5A6.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07480_A5A6a.seq check
>07480_07480_A5A6_pGL4-4174F_A01_01.ab1
    1 TTCTGTTTCC AGGACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
   61 GGATTCTTGA AACCTCGCAT GCTTCACCGT TTAATAAAAT TATGACTCAT TTTAGTAAGA
  121 ACATGCTTTT TTAGTAAGAA AAAATGCTTT CTGAGCTAAG TTTGAACTGC TGGGGCACAT
  181 GGGACACCCA CAAACCCAGT CAGCAGAAGT CTCAAACTCC TAAAAGATTA AAGGAAAAAT
  241 ACATTAGTGT GTACAGAGAA TGAAAAAAAA ATCCCCAATG GTTCTAATCT ATAAACGGAA
  301 GTAAAACTAA AACTCCCCTA AAATCCCCCC AGTCATCAAT CCTTATAAAT TCAGCACTTA
  361 TCTTCTTCCT TTACTTCTGG AGACCCTCAT TAAACAACAC GAAAGCCTTT GCTGACTTTT
  421 ATCTCGTAAA ATAGAGCCCT CAAATGACAC TAAGCAGGGA TACCGACCTC CAAAATCACA
  481 AACTGTTTTA CTTCTAAAAC CATGCATGCA AACGTGGGGT TTCCTGAGTT CTGCTAGGGG
  541 CGGGCTGTCT GGACCCCCGT GTATTTTTTT TTTTTTTTTT TTTTTTTTTT TTTGCAGGTG
  601 TGCAATCCAG GGTGCGCGCT CTGTGCAGGG AGGGGAGCAG AAGTGTGCGG TGGGCACCGC
  661 CTGGGCCGGG CGGGGCGGGG TGGGTGGGGC CCGGGTGTGT GCTGGGGTGA GAGTGAAGGA
  721 CATGTGACAA TACGACGGTG GCGGCTGCAG GATCCGGGAG GGGAGTGAAA GCTCCGCTGG
  781 GGCGCTCCAT TAGTGAGCGG TGATGGTTGC CGCCCACTTG TATTGAAGCG TCCTTTGTCA
  841 CTAACAAGTT AATTAGAGAT GTTATTTATT TAAGAAGAAG GAAAAAAAAA CCCTAGCCAA
  901 ACAGCCTATG AATACATATG CTCACATCCC CCTACTCCTC CAACTCCCTA ATTTTCCCCC
  961 GTCTCCAGAG GGGCACAGTT GTAAACCTTG TACGAAAAAT TCCAATTCTT TCTTGGGTGC
 1021 GGGGAATTTT CCCCCCCCAC CGGCTTTGCC GCCCCCCCGC CGAAAGTGGA GTTGGGGGAG
 1081 CTGGGAAGGG AGCTCTCTTG GGTCCCCGCT GGTCTCCGGG AGGCACCGCG GGGAAGCCGG
 1141 GGGCGGACGC GGGGGGTGGC GGGTCAGGCC TAGGGAGGGT AGTATGGATG GTCTCGTCCT
1201 CGGGGAACCT ATGTGGGGGG CCGGCACGTC GGGCGGT
//
Primer: pGL4-136R
Sequence file: RDB07480_A5A6b.seq check
>07480_07480_A5A6_pGL4-136R_B01_04.ab1
    1 GCCATTCCAA GTGTTTTGGC ATCTTCCATG GTGGCTTTAC CAACAGTACC GGATTGCCAA
   61 GCTTGGCCGC CGAGGCCAGA TCTTGATGAG GAGGAGGAAA CAGGTTGATA TTAAAGTGTA
  121 AACTCTGTAA ACAGAGATGC CATCAAACAA AGAGCTTTGG ACACTCCCCC TCCCGCCAAA
  181 TCTGGCGCCC CTGCAGTGCG CACGCGCCCT GCCCGCCGCC TCCGCGGTCG CCCTGGCTGC
  241 CGCCCGCGGG GCTGCCGGGC TGGCCGGGTC GCCGCTCCCA TTCGGCGCTC GCGCCGCCGC
  301 GGTGCGCCTA GCGCGCCGCG AGGCCCGGGA GGCGCGCGGG CAGGGAGCCC GGGGCCAGGG
  361 AGGGGTGGCC GGGGGCCGCG ACGGCGCCTT TTCCTGAGCA AACCCCGCTC CCGGGTCGCG
  421 GCGGCGCCGA CCCGCGCCCA GTCCCGCCGC CTCCCCTCCT CCCTGGCTGC CGCACCGCGG
  481 TCGCCCGGCT CCGCGTGCTC CCGGGCAGCG GGACCAGAGC TCCTCCAGCT CCCACTCACT
  541 GTCGCGGGGG CGGCAAGCGG TGGGGGGAAA TTCCCGCACA GAAGATTGGA TTTTCGTCAA
  601 GGTTTACAAC TGTGCCCTCT GGAGACGGGG AAATTAGGAG TTGGAGGAGT AGGGGATGTG
  661 AGCATATGTA TTCATAGGCT GTTTGGCTAG GGTTTTTTTT CCTTCTTCTT AAATAAATAA
  721 CATCTCTAAT TTAACTTGTT AGTGACAAAG GACGCTTCAA TACAAGTGGG CGGCAACCAT
  781 CACCGCTCAC TAATGGAGCG CCCCAGCGGA GCTTTCACTC CCCTCCCGGA TCCCTGCAGC
  841 CGCCACCGTC GTATTGTCAC ATGTCCTTTC ACTCTCACCC CAGCACACAC CCGGGCCCCA
  901 CCCAACCCCG CCCCGCCCGG CCCAAGGCGG TGCCCCACCG CACACCCTCT GCTCCCCCTC
  961 CCTGCACAGA GCGCGGCACC CCTGGAATTT GCCACACCCT GGCAAAAAAA AAAAAAAAAA
 1021 AAAAAAAAAA TACCACGGGG GGTTCCAGAA CAGCCCCGCC CCCTAACCAG AACCTCAGGA
 1081 AACCCCCCAC GGTTTGGCCC ATGGCCATGG GTTTTTAGAG AAGTAAAAAA CCGAGTTTTG
 1141 GTGAATTTTT GGGAAGGTTC CCGGGTTAAT TCCCCTCGTG TCCTTTAATA AGGGGGGTCT
1201 CAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles