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pGL4-phCOL1A1 (#RDB07459)

Promoter collection, Human COL1A1 promoter

Alternative name U2774-2
Clone info. PCR amplified human COL1A1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 50202872 to 50201613(NC_000017.11); relatively -1233 to +27, where +1 corresponds to 1 nt of NM_000088.3.
Comment PCR cloning, forward primer: CAGTGGGAAGTAGCCCCTAA; reverse primer: AGGAGAAACTCCCGTCTGCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human collagen, type I, alpha 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07459.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07459 pGL4-phCOL1A1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCOL1A1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07459).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07459_A1Htp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site,insert 5'
Sequence file: RDB07459_A1Htd.seq check
>07459_07459_B1HbF_pGL4ins_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNTCCC CAGNGCAAGT GCAGGTGCCA
   61 GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG GATCAGTGGG
  121 AAGTAGCCCC TAAAACAAAG TCAATCATCC TCTGCAGCCC ATCCCACACC CCCAAAGGAA
  181 AGTTTCACCC AGACACCCAA AATATCCCAT ACATCCCCAA CACTGAGTCC AGGTACAACT
  241 GGAGAAGGGG CTTTATGCAG CTCCCAGAAA GACACCCCTT TAGCTAAGTG CCCTCCCTCC
  301 ACCCAGGTTC TCTCTGGTTT GACTGTGCTG GGAAGGAGGG TCTCTAAGCA GCCCCTGGCC
  361 ACAGCCATGG CAAACAAAAC TCTTCTCTAA GTCACCAATG ATCACAGGCC TCCCACTAAA
  421 AATACTTCCC AACTCTGGGG TGGAAGAGTT TGGGGGATGA ATTTTTANGN NNTTGCAAGC
  481 CCCAATCCCC ACCTCTGTGT CCCTAGAATC CCCCACCCCT ACCTTGGCTG CTCCATCACC
  541 CAACCACCAA AGCTTTCTTC TGCAGAGGCC ACCTAGTCAT GTTTCTCACC CTGCACCTCA
  601 GCCTCCCCAC TCCATCTCTC AATCATGCCT ANGGNTTGGA NGAANGCANT TGATTCTGTT
  661 CTGGANCACA GCANAAGAAN TGACATCCTC AAAANTAAAA CTCCCNNNNN TGNACCCCTC
721 CNNNNNANNT CTGANNNNNN ANNNNNNNNN N
//
Primer: pGL4ins_R (Pr0666)
Region: Luc 5',insert 3'
Sequence file: RDB07459_A1Hte.seq check
>07459_07459_B1HbF_pGL4ins_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGGGT NNNNNNCGCT GGGCCCTTCT TAATGTTTTT GGCATCTTCC
   61 ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC AGATCTTGAT
  121 AGGAGAAACT CCCGTCTGCT CCGACGACTG GCCCGGGCCC CTTTTATACT GTCCTGATGG
  181 AGAGCAGGGA GGAACCCTGC CCCTCGGAGA GGGGGAGCCG GCCGCCCGTG CCCCAGCCAA
  241 TCAGAGCTGC CTGGCCCGGC CCCCAATTTG GGAGTTGGAA TGGAGAGGGG GAGGAGGAGG
301 GAAGGGNNNN NNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 pA,Luc 3'
Sequence file: RDB07459_A1Htf.seq check
>07459_07459_B1HbF_SV40pA_R_F01_06_ABI08.ab1
    1 NNNNNNNNCN NNNATTTNNN NAATAAAGCA TTTTTTTNNC TGCATTCTAG TTGTGGTTTG
   61 TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA
  121 GAATTATTAC ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT
  181 GCGGGCGTCC AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC
  241 GCGCAGCTTC TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT
  301 CATGGTTTTA CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC
  361 GGGCAGGCCG GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG
  421 TTCGGCTGGG GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT
  481 GAAGAAGTGC TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT
  541 GTCGATGAGA GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC
  601 ACGGACGCAC AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCNAGTCCAC
  661 CACCTTANCC TCNAANANNN GNCANCANCN TNGNCNACTG CGNNAGGNTT GNCGTCCCNT
721 TCNGGGGNGN NNNNANTNNN NNNNGNNNNN TNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles