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pGL4-phLIF (#RDB07417)

Promoter collection, Human LIF promoter

Alternative name U3312-4
Clone info. PCR amplified human LIF promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 30248070 to 30246767(NC_000022.11); relatively -1219 to +85, where +1 corresponds to 1 nt of NM_002309.4.
Comment PCR cloning, forward primer: CTGGCCTTGGAAAACCAACT; reverse primer: TGGGGAATTTGCCTGATTTA. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human leukemia inhibitory factor (cholinergic differentiation factor) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07417.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB07417 pGL4-phLIF Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pGL4-phLIF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07417).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles