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pGL4-phTP53AIP1 (#RDB07410)

Promoter collection, Human TP53AIP1 promoter

Alternative name U3302-15
Clone info. PCR amplified human TP53AIP1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 128944146 to 128942842(NC_000011.10); relatively -747 to +558, where +1 corresponds to 1 nt of NM_022112.2.
Comment PCR cloning, forward primer: CCAAAGCTCCACACCTCTTG; reverse primer: ATCTGTGCTGCTGTGACTGG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human tumor protein p53 regulated apoptosis inducing protein 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07410.pdf

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RDB07410 pGL4-phTP53AIP1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phTP53AIP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07410).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07410_A3Df.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07410_A3Dfa.seq check
>07410_07410_A3Df_pGL4-4174F_C01_07.ab1
    1 AGGGGGTCCA GACATTTCTC TGGCCTACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
   61 TCCAAAGCTC CACACCTCTT GCTATCCCAG ACTCTCTGGA ACACCAGGAA GAAAACATAT
  121 TTGGACAAAA ATTATTCTTT GCAACATTAG AACTTTTGAT TCTGGAATGA GACAAGGAAA
  181 TATGTATGTA TCTCAGAGAG ACAATGAATT TTTAAAAGGA AATTGGGACC GTTTCTCAGG
  241 GTGAGATGTC TTATCCGGTT AACTGCGAGC CCCCAGGGAT TTCTACTCAG AGTGTTCATC
  301 ACATCAGGTT TCCATTCTCT ACAATAAAAA CATGGCTAGG GAGAAATTAC CCAGCACTGG
  361 CAAGAAAGAG CTCCAAAATA GAAGCAGCTT TTGAGGAATT TGGAGGGATA AAACGGAATA
  421 GTATGTCTGA AAGTTGGCAA CATGTAAAAA GGCTAAAAAT GACAATTCTG CCTGTGATAC
  481 TATGTCTAGT GTGCAACGCT TCATTCAAAA GGCAGGCACG TCTCCCCTCC AGGCGGGAGT
  541 GGGAATCTCA GTGGGGAACC GGGAACTGCG TTTGAGCTGA TGCAGACTCA GGGTCAACTC
  601 GACAAGGGAG CCCGGCACCC AGACACAGCG GCGTTCACCT CACCTTGCAC CGTCTTTGTC
  661 GGTCTTACAG GCATCAGCTT TCCCAGCCTC ATTTGAGCCG GGATCTGAGT TGCATTTCTT
  721 TCTGGGTCAG GCCTTGCCAT TAGGGAGAGA TGAAGTCAAC TGATGAGCGT GCCAGCCTTG
  781 AGGAAGGACA GAGTGCGGGA AGCAGAGTGT GGGAAGTTGG AAAAAAATAA TCTGCAAGGT
  841 AAACACCTTC AAGTCAATTT CAAGAGGCTG GCTAGAAACC GCCAGGACTT CAGGGGGCCC
  901 TGTCTCGCGG GTCTCCCTGC GCCCTCAGCA CTTTGGCTTG GTATAAGGCC ACCTTGTCCC
  961 TGAAGAGAGG CCTATTTTTA AGCTGAGCAA GAAAAAGGTG AGAAGACTAA AAATCAGCTT
 1021 TTGTGCAGAG ACGCCCAAAT TTTCTGCTGC ACCACATGGT AAACGATGTG CACATTACAG
1081 CGAGCTTGGC CAAGCTCCCG GACCACGATG GGCGTCCAAA CACTGGT
//
Primer: pGL4-136R
Sequence file: RDB07410_A3Dfb.seq check
>07410_07410_A3Df_pGL4-136R_D01_10.ab1
    1 GTCTAAGTTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
   61 GCCGCCGAGG CCAGATCTTG ATATCTGTGC TGCTGTGACT GGGTGCAGGT CTGAGCAGAG
  121 GCTTTCGGAG TGAGCTGAGC AGGCGAGCCC GAGCACGGCC TCTCAGGGAG CGAGCCCATA
  181 GGCTGGGGAG CCGTCCCTGG AGATGCCCCT GGGCTCCTTG CGGGGCATGG CTGAGTTCCT
  241 GCCTGTCCTT GGCAGGCCCG CAGTGGTGGG GTGGCTCAGG CAGTTACCCT TTTCACAACC
  301 AGTGCTGCTG GGGGTGAAGG CAACGGGTGG GTGAGCACGG ACAGTGTGAG CCACGTGGCC
  361 GGGAGCTGGG CCAGCTCGCT GTATGTGCAC ATCGTTTCCA TGTGTGCAGC AGAAATTGGC
  421 GTCTCTGCAC AAAGCTGATT TTAGTCTTTC TCACCTTTTC TTGCTCAGCT TAAAATAGGC
  481 CTCTCTTCAG GGACAAGGTG GCCTTATACC AAGCCAAAGT GCTGAGGGCG CAGGGAGACC
  541 CGCGAGACAG GGCCCCCTGA AGTCCTGGCG GTTTCTAGCC AGCCTCTTGA AATTGACTTG
  601 AAGGTGTTTA CCTTGCAGAT TATTTTTTCC AACTTCCCAC ACTCTGCTTC CCGCACTCTG
  661 TCCTTCCTCA AGGCTGGCAC GCTCATCAGT TGACTTCATC TCTCCCTAAT GGCAAGGCCT
  721 GACCCAGAAA GAAATGCAAC TCAGATCCCG GCTCAAATGA GGCTGGGAAA GCTGATGCCT
  781 GTAAGACCGA CAAAGACGGT GCAAGGTGAG GTGAACGCCG CTGTGTCTGG GTGCCGGGCT
  841 CCCTTGTCGA GTTGACCCTG AGTCTGCATC AGCTCAAACG CAGTTCCCGG TTCCCCACTG
  901 AGATTCCCAC TCCCGCCTGG AGGGGAGACG TGCCTGCCTT TTTGAATGAA GCGTTGCACA
  961 CTAGACATAG TATCACAGGC AGAAATTGTC ATTTTTTAGC CTTTTTTACA TGTTTGCCAA
 1021 ACTTTTCAGA CATACTATTT CCCGTTTTTA TCCCCTCCTA AATTTCCCTC AAAAGCTGCT
1081 TCTATTTTGC AGGCTCTTTC TGCCAGTGCT GGGGTTAATT TTTCTT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Igarashi, H., Anti-apoptotic roles for the mutant p53R248Q through suppression of p53-regulated apoptosis-inducing protein 1 in the RA-derived fibroblast-like synoviocyte cell line MH7A. Clin. Immunol. 150 (1): 12-21 (2014). PMID 24316591. [link to RRC of NBRP]