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pGL4-phEGFR (#RDB07398)

Promoter collection, Human EGFR promoter

Alternative name U2676(2054)-10
Clone info. PCR amplified human EGFR promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 55017912 to 55019251(NC_000007.14); relatively -1105 to +235, where +1 corresponds to 1 nt of NM_005228.5.
Comment PCR cloning, forward primer: AGCAAAGGGCAGGTCTGTAG; reverse primer: GCTCTCCCGATCAATACTGG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07398.pdf

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RDB07398 pGL4-phEGFR DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phEGFR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07398).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07398_A6Ddp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07398_A6Dda.seq check
>07398_07398_A6Dd_pGL4-4174F_A01_01.ab1
    1 AATGGGTCAG TACTTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
   61 TAGCAAAGGG CAGGTCTGTA GCTTCACTTA CTTCAGGATT GTGATTTTTG ACAGAGCCGA
  121 GAGATCAGGG TTGTTGAACC AGGCCTGAAG GTCCTAGTGA ATCTCGTGAA GAGAGGAGGG
  181 GTCTGGCTGT AACATGGACC TAGAGGACAT TTTTACTGCA GGAGAAGGAA CAGTGGGGAT
  241 GGGGTGGACT TGCCAAAGGA ATATAGCTCA AGTTCCTGCA GCCCAAAAAA GCTCAGTTTC
  301 TTTTGGCCAA AGCTTCCGCG AGTTTCCCTG GCATTTCTCC TGCGGGAGCT ACAGGGGCAG
  361 TGGGACACTT AGCCTCTCTA AAAGCACCTC CACGGCTGTT TGTGTCAAGC CTTTATTCCA
  421 AGAGCTTCAC TTTTGCGAAG TAATGTGCTT CACACATTGG CTTCAAAGTA CCCATGGCTG
  481 GTTGCAATAA ACATTAAGGA GGCCTGTCTC TGCACCCGGA GTTGGGTGCC CTCATTTCAG
  541 ATGATTTCGA GGGTGCTTGA CAAGATCTGA AGGACCCTCG GACTTTAGAG CACCACCTCG
  601 GACGCCTGGC ACCCCTGCCG CGCGGGCACG GCGACCTCCT CAGCTGCCAG GCCAGCCTCT
  661 GATCCCCGCG AGGGTCCCGT AGTGCTGCAG GGGAGGTGGG GACCCGAATA AAGGAGCAGT
  721 TTCCCCGTCG GTGCCATTAT CCGACGCTGG CTCTAAGGCT CGGCCAGTCT GTCTAAAGCT
  781 GGTACAAGTT TGCTTTGTAA AACAAAAGAA GGGAAAGGGG GAAGGGGACC CTGGCACAGA
  841 TTTGGCTCGA CCTGGACATA GGCTGGGCCT GCAAGTCCGC GGGGACCGGG TCCAGAGGGG
  901 CAGTGCTGGG AACGCCCCTC TCGGAAATTA ACTCCTCAGG GCACCCGCTC CCCTCCCATG
  961 CGCCGCCCCA CTCCCGCCGG GAGACTAGGT CCCGCGGGGG GCCCACCGCT GTCCACCGCC
 1021 CTCCGGCGGC CGGCTGGTCT TTGGTCCCCG CTGCTGATCC TCTCCCTTCT TCTTCGCAAT
1081 CTCCTCCTCC ATCTGCTTCC TTCCCGAATT CCATCCATCC CGACCGACCT TG
//
Primer: pGL4-136R
Sequence file: RDB07398_A6Ddb.seq check
>07398_07398_A6Dd_pGL4-136R_B01_04.ab1
    1 GCTATGTTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
   61 CCGCCGAGGC CAGATCTTGA TGCTCTCCCG ATCAATACTG GACGGAGTCA GGGGGCCGTG
  121 CGCGGTGGTT GTGGCGTTGG CGGCGAGGCG GGGACTCGGG CGGACGCCGA CGAGGTGGCC
  181 TGTCGTCCGG TCTGGGCGGC GGCGGCCGCC GGGGCTAGCT CGGGACTCCG GCCGCCTCGG
  241 CCGCGGCGGG CGCTCACACC GTGCGGGGGG AGGAGGCTGC TGCGGCCGCG CTGCGCCGGG
  301 GGCTGCCCGG ACGTCTAGCT CGCGCGGGCC GAGGCGCGGG GAGGCAGGGC GGGAGGAGGA
  361 GGGACCAGGC GGCGGAGGAG GGATCGGGAG GAGCAGAGGA GGAGGAGAAT GCGAGGAGGA
  421 GGGAGGAGAA CCAGCAGCGG GGACCCAAGG CCAGCGGCCG CCGGAGGCGG TGGACAGCGG
  481 TGGCCCCCGC GGGACCTAGT CTCCGGCGGG AGTGGGGCGG CGCATGGGAG GGGAGCGGGT
  541 GCCCTGAGGA GTTAATTTCC GAGAGGGGCG TTCCCAGCAC TGCCCCTCTG GACCCGGTCC
  601 CCGCGGACTT GCAGGCCCAG CCTATGTCCA GGTCGAGCCA AATCTGTGCC AGGGTCCCCT
  661 TCCCCCTTTC CCTTCTTTTG TTTTACAAAG CAAACTTGTA CCAGCTTTAG ACAGACTGGC
  721 CGAGCCTTAG AGCCAGCGTC GGGATAATGG CACCGACGGG GAAACTGCTC CTTTATTCGG
  781 GGTCCCCACC TCCCCTGCAG CACTACGGGG ACCCTCGCGG GGGGATCAGA GGCTGGGCCT
  841 GGGCAGCTGG AGGAGGGTCG CCGTGCCCGC GCGGGCAGGG GGGTGCCAGG GCGGTCCGAG
  901 GGTGGTGGCT CTAAAGTTCC GAGGGGTCCT TCAGATCTTT GTCAAGCACC CCTCGAAATC
  961 ATCTGAAATG AGGGGCACCC CAACTCCGGG TTGCAAGACA GGGCCTTCCT TTTAATGTTA
 1021 TTTGCGACCC AGCCCATGGG GTTACTTTTG AAGCCAATGG TGTGGAAGCA CCATTACTTC
 1081 GCAAAAAGTG GAAGCTTCTT GGAAATAAAG GGCTTGACCC AACAGCCGTG AAGGTGCCTT
1141 TATGAAAG
//
Primer: pAxCALNL_F1
Sequence file: RDB07398_A6Ddc.seq check
>07398_07398_A6Dd_pAxCALNL_F1_C01_07.ab1
    1 TCAAATCTTT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
   61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
  121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
  181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
  241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
  301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
  361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
  421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
  481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
  541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
  601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
  661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
  721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
  781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
  841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
  901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT
  961 GAACATGCCG AAGCCCGTGG TGAAATGGGC ACCCACGCTG AGGATAGCGG TGTCGGGGGA
 1021 TGATCCTGGT TTGCCGAAGA TGGGGGTCGC GGGCCATGGA CTGAATCGGG ACACAGCAGT
1081 GCCGTTGCCG GTTAAGGACT TACGGCCCTT TTGGGTCAAT CCCGGATTAA CCTGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles