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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV_s-FLAGc

Destination vector to generate mammalian expression vector, with FLAG-tag.

Catalog number RDB07396
Resource name pCMV_s-FLAGc
Clone info. Destination vector to generate mammalian expression vector, with FLAG-tag. Derived from pCMV_S-FLAG (RDB05956). Gateway compatible.
Vector backbone Plasmid
Size of vector backbone 7.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer DNA Bank, | Murata, Takehide |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use DB3.1(Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。発表に際し、理研BRCから入手したことを明記すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。

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            RDB07396 pCMV_s-FLAGc DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB07396zzt02.seq compiled by us
            Remarks, protocol and/or map (pdf) RDB07396.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12331_A5J8p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB12331_A5J8a.seq check
            >07396_12331_A5J8_CMV-Forward_A09_03.ab1
                1 ACGAACAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
               61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
              121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
              181 AGGCGGCCAT CACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA
              241 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC
              301 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
              361 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC
              421 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA
              481 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT
              541 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT
              601 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA
              661 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC
              721 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT
              781 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT
              841 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA
              901 CGTGGCCAAT ATGGACAACT TCTTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC
              961 AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGGT TTGTGATGGC
             1021 TTCCATGTCG GCAGAATGCT TAAATGAATT TACAACAGTA CTGCGATGAG TGGCAGGCGG
             1081 GCGTAAACGC GTGGAATTCT GCTAACTAAA GTCAAGAATA ACAGGTAATG CGTATTTTGG
            1141 CGCGCGCCTT GAATATTTTT GTGGCGGG
            //
            Primer: BGH_rev
            Sequence file: RDB12331_A5J8b.seq check
            >07396_12331_A5J8_BGHrev_B09_06.ab1
                1 GTTCTTGCAA TTTAGGTGAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCAT
               61 CACCACTTTG TACAAGAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT
              121 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC
              181 ACTATGGTCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA
              241 ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT
              301 CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT
              361 CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC AGACGTGCAC
              421 TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA
              481 CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG
              541 CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC
              601 CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG
              661 GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT
              721 CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTCG
              781 GGTATACATA TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT ACGCATACTG
              841 TTATCTGGCT TTTAGTAAGC CGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA
              901 GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG
              961 AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGG
             1021 TGAAAACGGG GGGCGAAGAA AGTTGTCCAT ATTGGCCACG TTTTAAATCA AAACTGATGA
             1081 AACTCCACCC AGGGGATTTG ACCTGAGACG GAAAAACAAT ATTTCTCAAT AAACCCCTTT
            1141 TAAGGGGAAA TATAGGGCCA G
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB12331_A5J8c.seq check
            >07396_12331_A5J8_SV40pro_F_V2_C09_09.ab1
                1 GTACCGGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG GATATCCATT
               61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
              121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
              181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
              241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
              301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
              361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
              421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
              481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
              541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
              601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
              661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
              721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
              781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
              841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
              901 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT
              961 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCCGGCTGG
             1021 ATGATCCCTC CAGCGCGGGG GATCTCATGC TGGAGTTCTC GCCCACCCCA ACATGTTAAT
             1081 GCCAGCTTAT ATGGTTACCA ATAAAGCATA GCATTCCAAC AAATTCCAAC AAAAATAAAG
            1141 CCATTTTTTT TTTTCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl