Resource data sheet
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pCMV_s-FLAGc

Destination vector to generate mammalian expression vector, with FLAG-tag.

Catalog number RDB07396
Resource name pCMV_s-FLAGc
Clone info. Destination vector to generate mammalian expression vector, with FLAG-tag. Gateway compatible.
Vector backbone Plasmid
Size of vector backbone 7.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor DNA Bank, | Murata, Takehide |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
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Material Transfer Agreement (MTA) [Word]
Remarks Use DB3.1(Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07396 pCMV_s-FLAGc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07396z.seq from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB07396.pdf from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12331_A5J8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward | Sequence: RDB12331_A5J8a.seq
>07396_12331_A5J8_CMV-Forward_A09_03.ab1
    1 ACGAACAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
  181 AGGCGGCCAT CACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA
  241 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC
  301 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
  361 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC
  421 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA
  481 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT
  541 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT
  601 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA
  661 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC
  721 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT
  781 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT
  841 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA
  901 CGTGGCCAAT ATGGACAACT TCTTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC
  961 AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGGT TTGTGATGGC
 1021 TTCCATGTCG GCAGAATGCT TAAATGAATT TACAACAGTA CTGCGATGAG TGGCAGGCGG
 1081 GCGTAAACGC GTGGAATTCT GCTAACTAAA GTCAAGAATA ACAGGTAATG CGTATTTTGG
1141 CGCGCGCCTT GAATATTTTT GTGGCGGG
//
Primer: BGH_rev | Sequence: RDB12331_A5J8b.seq
>07396_12331_A5J8_BGHrev_B09_06.ab1
    1 GTTCTTGCAA TTTAGGTGAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCAT
   61 CACCACTTTG TACAAGAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT
  121 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC
  181 ACTATGGTCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA
  241 ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT
  301 CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT
  361 CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC AGACGTGCAC
  421 TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA
  481 CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG
  541 CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC
  601 CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG
  661 GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT
  721 CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTCG
  781 GGTATACATA TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT ACGCATACTG
  841 TTATCTGGCT TTTAGTAAGC CGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA
  901 GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG
  961 AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGG
 1021 TGAAAACGGG GGGCGAAGAA AGTTGTCCAT ATTGGCCACG TTTTAAATCA AAACTGATGA
 1081 AACTCCACCC AGGGGATTTG ACCTGAGACG GAAAAACAAT ATTTCTCAAT AAACCCCTTT
1141 TAAGGGGAAA TATAGGGCCA G
//
Primer: SV40pro_F_V2 | Sequence: RDB12331_A5J8c.seq
>07396_12331_A5J8_SV40pro_F_V2_C09_09.ab1
    1 GTACCGGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG GATATCCATT
   61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
  661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
  721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
  781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
  841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
  901 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT
  961 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCCGGCTGG
 1021 ATGATCCCTC CAGCGCGGGG GATCTCATGC TGGAGTTCTC GCCCACCCCA ACATGTTAAT
 1081 GCCAGCTTAT ATGGTTACCA ATAAAGCATA GCATTCCAAC AAATTCCAAC AAAAATAAAG
1141 CCATTTTTTT TTTTCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.11.02

GNP_filter3_RDBDEP_html_190927.pl