RDB07396 - pCMV

Resource data sheet
Prev.

pCMV_s-FLAGc

Destination vector to generate mammalian expression vector, with FLAG-tag.

Catalog number	RDB07396
Resource name	pCMV_s-FLAGc
Clone info.	Destination vector to generate mammalian expression vector, with FLAG-tag. Gateway compatible. Contains ccdB and use DB3.1 or an equivalent host bacteria.
Vector backbone	Plasmid
Size of vector backbone	7.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Depositor	DNA Bank, \| Murata, Takehide \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.

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Remarks Use DB3.1(Invitrogen) or equivalent to amplify this clone.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]
Remarks	Use DB3.1(Invitrogen) or equivalent to amplify this clone.

提供条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。発表に際し、理研BRCから入手したことを明記すること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]
備考	このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07396	pCMV_s-FLAGc	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB07396z.seq from Depositor
Electronic file	PDF RDB07396.pdf from Depositor

Featured content	pCMV_tag and pRSV_tag vector (English text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)
Featured content	pCMV_tag and pRSV_tag vector (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB12331_A5J8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward	RDB12331_A5J8a.seq
>07396_12331_A5J8_CMV-Forward_A09_03.ab1 1 ACGAACAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA 181 AGGCGGCCAT CACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA 241 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC 301 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 361 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC 421 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA 481 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT 541 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT 601 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA 661 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC 721 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT 781 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT 841 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA 901 CGTGGCCAAT ATGGACAACT TCTTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC 961 AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGGT TTGTGATGGC 1021 TTCCATGTCG GCAGAATGCT TAAATGAATT TACAACAGTA CTGCGATGAG TGGCAGGCGG 1081 GCGTAAACGC GTGGAATTCT GCTAACTAAA GTCAAGAATA ACAGGTAATG CGTATTTTGG 1141 CGCGCGCCTT GAATATTTTT GTGGCGGG //
Primer: BGH_rev	RDB12331_A5J8b.seq
>07396_12331_A5J8_BGHrev_B09_06.ab1 1 GTTCTTGCAA TTTAGGTGAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCAT 61 CACCACTTTG TACAAGAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT 121 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC 181 ACTATGGTCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA 241 ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT 301 CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT 361 CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC AGACGTGCAC 421 TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA 481 CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG 541 CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC 601 CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG 661 GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT 721 CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTCG 781 GGTATACATA TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT ACGCATACTG 841 TTATCTGGCT TTTAGTAAGC CGGATCCACG CGTTTACGCC CCGCCCTGCC ACTCATCGCA 901 GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG 961 AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGG 1021 TGAAAACGGG GGGCGAAGAA AGTTGTCCAT ATTGGCCACG TTTTAAATCA AAACTGATGA 1081 AACTCCACCC AGGGGATTTG ACCTGAGACG GAAAAACAAT ATTTCTCAAT AAACCCCTTT 1141 TAAGGGGAAA TATAGGGCCA G //
Primer: SV40pro_F_V2	RDB12331_A5J8c.seq
>07396_12331_A5J8_SV40pro_F_V2_C09_09.ab1 1 GTACCGGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG GATATCCATT 61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 901 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 961 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCCGGCTGG 1021 ATGATCCCTC CAGCGCGGGG GATCTCATGC TGGAGTTCTC GCCCACCCCA ACATGTTAAT 1081 GCCAGCTTAT ATGGTTACCA ATAAAGCATA GCATTCCAAC AAATTCCAAC AAAAATAAAG 1141 CCATTTTTTT TTTTCA //

Please visit Sequencing and PCR primers for primer information.

References

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl