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pGL4-phINS (#RDB07387)

Promoter collection, Human INS promoter

Alternative name U2643 (2021)-16
Clone info. PCR amplified human INS promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 2162444 to 2161182(NC_000011.10); relatively -1235 to +28, where +1 corresponds to 1 nt of NM_000207.3.
Comment PCR cloning, forward primer: acctccaggtgcctgttct; reverse primer: cttctgatgcagcctgtcct. Entire sequence of promoter region has not been confirmed. Known difference (at least) : G to C substitution at 2161265nt on NC_000011.10.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human insulin genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07387.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07387 pGL4-phINS DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phINS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07387).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07387_B4Bkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site,insert 5'
Sequence file: RDB07387_B4Bka.seq check
>07387_07387_B4Bk_3_pGL4ins_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNTAGNNN ANNAGNCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT
   61 TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC GAGGATACCT CCAGGTGCCT
  121 GTTCTGGGGA GCTGGGAGGG CCGGAGGGGT GTACCCCAGG GGCTCAGCCC AGATGACACT
  181 ATGGGGGTGA TGGTGTCATG GGACCTGGCC AGGAGAGGGG AGATGGGCTC CCAGAAGAGG
  241 AGTGGGGGCT GAGAGGGTGC CTGGGGGGCC AGGACGGAGC TGGGCCAGTG CACAGCTTCC
  301 CACACCTGCC CACCCCCAGA GTCCTGCCGC CACCCCCAGA TCACACGGAA GATGAGGTCC
  361 GAGTGGCCTG CTGAGGACTT GCTGCTTGTC CCCAGGTCCC CAGGTCATGC CCTCCTTCTG
  421 CCACCCTGGG GAGCTGAGGG CCTCAGCTGG GGCTGCTGTC CTAAGGCAGG GTGGGAACTA
  481 GGCAGCCAGC AGGGAGGGGA CCCCTCCCTC ACTCCCACTC TNNNNNNCCC ACCACCTTGG
  541 CCCATCCATG GCGGCATCTT GGNNNNNNCG GGANTGGGGA CAGGGGTCCT GGGGNNNNNN
  601 NGTCCGGGGA CNNGGTCCTG GGGNCAGGGN TGNGAGGANA GGGGTCCCGG GGANNGGGGN
661 NGNNGGGGNN NNNNGTNCNN GNNNNNNNNG NNTGTNGNNN ANNNGNNNNN NNNNN
//
Primer: pGL4ins_R (Pr0666)
Region: Luc,insert 3'
Sequence file: RDB07387_B4Bkb.seq check
>07387_07387_B4Bk_3_pGL4ins_R_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNANNNNG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT
   61 CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC TTGGCCGCCG AGGCCAGATC
  121 TTGATCTTCT GATGCAGCCT GTCCTGGAGG GCTGAGGGCT GCTGGGCCCC CGCTGGCTTT
  181 ATAGTCTCAG AGCCCATCTC CCCTACCTGT CAACCCCTGC CGCCTGGCCC ATTAGGGCCT
  241 GGGGTGGGGG GGTCGGCAGA TGGCTGGGGG CTGAGGCTGC AATTTCCGGA CCATTTCCCT
  301 GGTGCTGGGT CTGTGGGAAG ATCTCCTTGG TCGTCAGCAC CTCTTCCTCA GGACCAGCGG
  361 GTCATTAGAG TCTTAACCAG GGGCCCGGTG GCCAGACCTG TCCCTGCTCA CAGCTGGGGG
  421 CAAATGTCTC CAGGAGAGCA AAGCCCTCAC CTGGGCCACT TTCCACATTA GACCAGGAGA
  481 GCTGGAGGCT GCAGGGCGGG GCTCTTTGCG CTGCTGTCCC CAGACCCCTG TCCCCAGGAC
  541 CCCTGTCCCC AGGACCCCTG TCCCCAGGAN CCCTGTCCCC ACACCCCTGT CCCCACACCC
  601 CTGTCCCCAC ACCCCTGTCN NNNNACCCCT GTCCCCNGGA NCCCTGTCCC CACACCCCTG
  661 TCCCCAGGAN CCCTGTCCCC ACANNNNTGT CCCCANGANC CCNGNNCCCA CANCNNNNNN
721 NCCNNNNCNN NNNNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 pA,Luc 3'
Sequence file: RDB07387_B4Bkc.seq check
>07387_07387_B4Bk_3_pGL4pA_R_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNATCGAN TTNNCCNCAT TTGTAGAGGT TTTACTTGCT
   61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGNTGTT
  121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
  181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
  241 TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT ACACGGCGAT
  301 CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT CCAACTTGCC
  361 GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT TCTTGGCGGT
  421 TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT TACCGTGTTC
  481 CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC CGGCGACCCC
  541 GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GANGCTCTCC AGTTCGGCTG GGGCTACCTG
  601 GTANCCCTTG TATTTGATNA GGCTCTTCNG CCGGTCCACG ATGAANAANT GCTCGTCCTC
  661 GTCCCANTNN NNGANGTCGC CGCTGNGCAN CCNNCCNTCC TTGNCNANGA NANCGNTTGN
721 NNNCNNCGGG NNNGNNNNNN NANNCNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles