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pGL4-phTERT (#RDB07379)

Promoter collection, Human TERT promoter

Alternative name U2740(2118)-12
Clone info. PCR amplified human TERT promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 1296353 to 1295013(NC_000005.10); relatively -1306 to +35, where +1 corresponds to 1 nt of NM_198253.2.
Comment PCR cloning, forward primer: CAAGACGAGGCTAACCTCCAG; reverse primer: CTTCCCACGTGCGCAGCAG. Entire sequence of promoter region has not been confirmed. Known difference (at least) : C to T substitution at 1295957nt.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human telomerase reverse transcriptase genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07379.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07379 pGL4-phTERT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phTERT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07379).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB20026_B3Gep1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: insert 5'
Sequence file: RDB20026_B3Gea.seq check
>07379_20026_B3Ge_1_pGL4ins_F_C01_03_ABI08.ab1
    1 NNNNNNNNNA NNNNNNAGNN NNNAGGCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT
   61 TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC GAGGNTCAAG ACGAGGCTAA
  121 CCTCCAGCGA GCGTGACAGC CCAGGGAGGG TGCGAGGCCT GTTCAAATGC TAGCTCCATA
  181 AATAAAGCAA TTTCCTCCGG CAGTTTCTGA AAGTAGGAAA GGTTACATTT AAGGTTGCGT
  241 TTGTTAGCAT TTCAGTGTTT GCCGACCTCA GCTACAGCAT CCCTGCAAGG CCTCGGGAGA
  301 CCCAGAAGTT TCTCGCCCCT TAGATCCAAA CTTGAGCAAC CCGGAGTCTG GATTCCTGGG
  361 AAGTCCTCAG CTGTCCTGCG GTTGTGCCGG GGCCCCAGGT CTGGAGGGGA CCAGTGGCCG
  421 TGTGGCTTCT ACTGCTGGGC TGGAAGTCGG GCCTCCTAGC TCTGCAGTCC GAGGCTTGGA
  481 GCCAGGTGCC TGGACCCCGA GGCTGCCCTC CACCCTGTGC GGGCGGGATG TGACCAGATG
  541 TTGGCCTCAT CTGCCAGACA GAGTGCCGGG GCCCAGGGTC AAGGCCGTTG TGGCTGGTGT
  601 GAGGCGCCCG GTGCGCGGCC AGCAGGAGCG CCTGGCTCCA TTTCCCACCC TTTCTCGACG
  661 GGACCGCCCC GGTGGGTGAN TAACAGATTT GGGGTGGTTT GCTCATGGTG GGGANCCCTC
  721 GCCGCCTGNN AACCTGCAAA GAGAAATGAC GGGCCTGTGT CNAGGANCCC AAGTCGCGGG
  781 GAANTGTTGC ANGGNNGCAC TCCGNNNNGT CCCGCGNGNC CGTCCAGGGN NCAATGCGNC
  841 CNCNGGTTCG NCCCCAGCCG CGNCNACNCN CCTCCNNCCT CCCNNNNACG TCNNGNNNTC
901 NNNGNNGCCC NGNNNCCNNN NNNCNCNNNN NNNGNNNNNN NNNN
//
Primer: pGL4ins_R (Pr0666)
Region: Luc 5',insert 3'
Sequence file: RDB20026_B3Geb.seq check
>07379_20026_B3Ge_1_pGL4ins_R_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNNNNNGGC GCTGGGCCCT TCTTAATGTT TTTGGCATCT
   61 TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
  121 GATCTTCCCA CGTGCGCAGC AGGACGCAGC GCTGCCTGAA ACTCGCGCCG CGAGGAGAGG
  181 GCGGGGCCGC GGAAAGGAAG GGGAGGGGCT GGGAGGGCCC GGAGGGGGCT GGGCCGGGGA
  241 CCCGGGAGGG GTCGGGACGG GGCGGGGTCC GCGCGGAGGA GGCGGAGCTG GAAGGTGAAG
  301 GGGCAGGACG GGTGCCCGGG TCCCCAGTCC CTCCGCCACG TGGGAAGCGC GGTCCTGGGC
  361 GTCTGTGCCC GCGAATCCAC TGGGAGCCCG GCCTGGCCCC GACAGCGCAG CTGCTCCGGG
  421 CGGACCCGGG GGTCTGGGCC GCGCTTCCCC GCCCGCGCGC CGCTCGCGCT CCCAGGGTGC
  481 AGGGACGCCA GCGAGGGCCC CAGCGGAGAG AGGTCGAATC GGCCTAGGCT GTGGGGTAAC
  541 CCGAGGGAGG GGCCATGATG TGGAGGCCCT GGGAACAGGT GCGTGCGGCG ACCCTTTGGC
  601 CGCTGGCCTG ATCCGGAGAN CCAGGGCTGC CTCCAGGTCC GGACGCGGGG CGTCNGGCTC
  661 CGGGCACCAC GAATGCCGGA CGTGAANGGG ANGNNGGANG CNCNNANNNN NNNNTGGGNN
721 NNNNNCNNNN NNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 pA,Luc 3'
Sequence file: RDB20026_B3Gec.seq check
>07379_20026_B3Ge_1_pGL4pA_R_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT
   61 AAAAAACCTC CNNNNNNNNC NCNNNNACCT GNAACATAAA ATGAATGCAA TTGNNNNNNN
  121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
  181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
  241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT
  301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG
  361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG
  421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA
  481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG
  541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT
  601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT
  661 CCCAGTANGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG
  721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC
  781 GCTGGTTCAC ACCCAGTGTC TTANCGGTGT CNNAGTCCAC CACCTTANCC TCNAANAANN
  841 NNNNCACCTT NCCTACTGCG CCNNGNTTGT CGNCCCCTNN NGGGGNGATC AGAANNGNNC
  901 NNNNNGNTTC TGTNNNNNNG TNNNNNNTGG NNNNNGNCNG GNANNTNNNA NNCNNTTNNN
961 NNCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles