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pGL4-phTERT

Promoter collection, Human TERT promoter

Catalog number RDB07379
Resource name pGL4-phTERT
Alternative name U2740(2118)-12
Clone info. PCR amplified human TERT promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 1296353 to 1295013(NC_000005.10); relatively -1306 to +35, where +1 corresponds to 1 nt of NM_198253.2.
Comment PCR cloning, forward primer: CAAGACGAGGCTAACCTCCAG; reverse primer: CTTCCCACGTGCGCAGCAG. Entire sequence of promoter region has not been confirmed. Known difference (at least) : C to T substitution at 1295957nt.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human telomerase reverse transcriptase genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human telomerase reverse transcriptase

          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07379.pdf

          Distribution information

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          RDB07379 pGL4-phTERT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phTERT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07379).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB20026_B3Gep1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F (Pr0665)
          Region: insert 5'
          Sequence file: RDB20026_B3Gea.seq check
          >07379_20026_B3Ge_1_pGL4ins_F_C01_03_ABI08.ab1
              1 NNNNNNNNNA NNNNNNAGNN NNNAGGCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT
             61 TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC GAGGNTCAAG ACGAGGCTAA
            121 CCTCCAGCGA GCGTGACAGC CCAGGGAGGG TGCGAGGCCT GTTCAAATGC TAGCTCCATA
            181 AATAAAGCAA TTTCCTCCGG CAGTTTCTGA AAGTAGGAAA GGTTACATTT AAGGTTGCGT
            241 TTGTTAGCAT TTCAGTGTTT GCCGACCTCA GCTACAGCAT CCCTGCAAGG CCTCGGGAGA
            301 CCCAGAAGTT TCTCGCCCCT TAGATCCAAA CTTGAGCAAC CCGGAGTCTG GATTCCTGGG
            361 AAGTCCTCAG CTGTCCTGCG GTTGTGCCGG GGCCCCAGGT CTGGAGGGGA CCAGTGGCCG
            421 TGTGGCTTCT ACTGCTGGGC TGGAAGTCGG GCCTCCTAGC TCTGCAGTCC GAGGCTTGGA
            481 GCCAGGTGCC TGGACCCCGA GGCTGCCCTC CACCCTGTGC GGGCGGGATG TGACCAGATG
            541 TTGGCCTCAT CTGCCAGACA GAGTGCCGGG GCCCAGGGTC AAGGCCGTTG TGGCTGGTGT
            601 GAGGCGCCCG GTGCGCGGCC AGCAGGAGCG CCTGGCTCCA TTTCCCACCC TTTCTCGACG
            661 GGACCGCCCC GGTGGGTGAN TAACAGATTT GGGGTGGTTT GCTCATGGTG GGGANCCCTC
            721 GCCGCCTGNN AACCTGCAAA GAGAAATGAC GGGCCTGTGT CNAGGANCCC AAGTCGCGGG
            781 GAANTGTTGC ANGGNNGCAC TCCGNNNNGT CCCGCGNGNC CGTCCAGGGN NCAATGCGNC
            841 CNCNGGTTCG NCCCCAGCCG CGNCNACNCN CCTCCNNCCT CCCNNNNACG TCNNGNNNTC
          901 NNNGNNGCCC NGNNNCCNNN NNNCNCNNNN NNNGNNNNNN NNNN
          //
          Primer: pGL4ins_R (Pr0666)
          Region: Luc 5',insert 3'
          Sequence file: RDB20026_B3Geb.seq check
          >07379_20026_B3Ge_1_pGL4ins_R_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG GNNNNNNGGC GCTGGGCCCT TCTTAATGTT TTTGGCATCT
             61 TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
            121 GATCTTCCCA CGTGCGCAGC AGGACGCAGC GCTGCCTGAA ACTCGCGCCG CGAGGAGAGG
            181 GCGGGGCCGC GGAAAGGAAG GGGAGGGGCT GGGAGGGCCC GGAGGGGGCT GGGCCGGGGA
            241 CCCGGGAGGG GTCGGGACGG GGCGGGGTCC GCGCGGAGGA GGCGGAGCTG GAAGGTGAAG
            301 GGGCAGGACG GGTGCCCGGG TCCCCAGTCC CTCCGCCACG TGGGAAGCGC GGTCCTGGGC
            361 GTCTGTGCCC GCGAATCCAC TGGGAGCCCG GCCTGGCCCC GACAGCGCAG CTGCTCCGGG
            421 CGGACCCGGG GGTCTGGGCC GCGCTTCCCC GCCCGCGCGC CGCTCGCGCT CCCAGGGTGC
            481 AGGGACGCCA GCGAGGGCCC CAGCGGAGAG AGGTCGAATC GGCCTAGGCT GTGGGGTAAC
            541 CCGAGGGAGG GGCCATGATG TGGAGGCCCT GGGAACAGGT GCGTGCGGCG ACCCTTTGGC
            601 CGCTGGCCTG ATCCGGAGAN CCAGGGCTGC CTCCAGGTCC GGACGCGGGG CGTCNGGCTC
            661 CGGGCACCAC GAATGCCGGA CGTGAANGGG ANGNNGGANG CNCNNANNNN NNNNTGGGNN
          721 NNNNNCNNNN NNNNNN
          //
          Primer: pGL4pA_R (Pr0664)
          Region: SV40 pA,Luc 3'
          Sequence file: RDB20026_B3Gec.seq check
          >07379_20026_B3Ge_1_pGL4pA_R_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT
             61 AAAAAACCTC CNNNNNNNNC NCNNNNACCT GNAACATAAA ATGAATGCAA TTGNNNNNNN
            121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
            181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
            241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT
            301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG
            361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG
            421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA
            481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG
            541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT
            601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT
            661 CCCAGTANGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG
            721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC
            781 GCTGGTTCAC ACCCAGTGTC TTANCGGTGT CNNAGTCCAC CACCTTANCC TCNAANAANN
            841 NNNNCACCTT NCCTACTGCG CCNNGNTTGT CGNCCCCTNN NGGGGNGATC AGAANNGNNC
            901 NNNNNGNTTC TGTNNNNNNG TNNNNNNTGG NNNNNGNCNG GNANNTNNNA NNCNNTTNNN
          961 NNCNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2023.08.01

          GNP_filter3_RDBDEP_html_230702.pl