RDB07379 - pGL4-phTERT

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pGL4-phTERT (#RDB07379)

Promoter collection, Human TERT promoter

Alternative name	U2740(2118)-12
Clone info.	PCR amplified human TERT promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 1296353 to 1295013(NC_000005.10); relatively -1306 to +35, where +1 corresponds to 1 nt of NM_198253.2.
Comment	PCR cloning, forward primer: CAAGACGAGGCTAACCTCCAG; reverse primer: CTTCCCACGTGCGCAGCAG. Entire sequence of promoter region has not been confirmed. Known difference (at least) : C to T substitution at 1295957nt.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human telomerase reverse transcriptase genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07379.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07379	pGL4-phTERT	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phTERT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07379).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB20026_B3Gep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665) Region: insert 5' Sequence file: RDB20026_B3Gea.seq
>07379_20026_B3Ge_1_pGL4ins_F_C01_03_ABI08.ab1 1 NNNNNNNNNA NNNNNNAGNN NNNAGGCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT 61 TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC GAGGNTCAAG ACGAGGCTAA 121 CCTCCAGCGA GCGTGACAGC CCAGGGAGGG TGCGAGGCCT GTTCAAATGC TAGCTCCATA 181 AATAAAGCAA TTTCCTCCGG CAGTTTCTGA AAGTAGGAAA GGTTACATTT AAGGTTGCGT 241 TTGTTAGCAT TTCAGTGTTT GCCGACCTCA GCTACAGCAT CCCTGCAAGG CCTCGGGAGA 301 CCCAGAAGTT TCTCGCCCCT TAGATCCAAA CTTGAGCAAC CCGGAGTCTG GATTCCTGGG 361 AAGTCCTCAG CTGTCCTGCG GTTGTGCCGG GGCCCCAGGT CTGGAGGGGA CCAGTGGCCG 421 TGTGGCTTCT ACTGCTGGGC TGGAAGTCGG GCCTCCTAGC TCTGCAGTCC GAGGCTTGGA 481 GCCAGGTGCC TGGACCCCGA GGCTGCCCTC CACCCTGTGC GGGCGGGATG TGACCAGATG 541 TTGGCCTCAT CTGCCAGACA GAGTGCCGGG GCCCAGGGTC AAGGCCGTTG TGGCTGGTGT 601 GAGGCGCCCG GTGCGCGGCC AGCAGGAGCG CCTGGCTCCA TTTCCCACCC TTTCTCGACG 661 GGACCGCCCC GGTGGGTGAN TAACAGATTT GGGGTGGTTT GCTCATGGTG GGGANCCCTC 721 GCCGCCTGNN AACCTGCAAA GAGAAATGAC GGGCCTGTGT CNAGGANCCC AAGTCGCGGG 781 GAANTGTTGC ANGGNNGCAC TCCGNNNNGT CCCGCGNGNC CGTCCAGGGN NCAATGCGNC 841 CNCNGGTTCG NCCCCAGCCG CGNCNACNCN CCTCCNNCCT CCCNNNNACG TCNNGNNNTC 901 NNNGNNGCCC NGNNNCCNNN NNNCNCNNNN NNNGNNNNNN NNNN //
Primer: pGL4ins_R (Pr0666) Region: Luc 5',insert 3' Sequence file: RDB20026_B3Geb.seq
>07379_20026_B3Ge_1_pGL4ins_R_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG GNNNNNNGGC GCTGGGCCCT TCTTAATGTT TTTGGCATCT 61 TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT 121 GATCTTCCCA CGTGCGCAGC AGGACGCAGC GCTGCCTGAA ACTCGCGCCG CGAGGAGAGG 181 GCGGGGCCGC GGAAAGGAAG GGGAGGGGCT GGGAGGGCCC GGAGGGGGCT GGGCCGGGGA 241 CCCGGGAGGG GTCGGGACGG GGCGGGGTCC GCGCGGAGGA GGCGGAGCTG GAAGGTGAAG 301 GGGCAGGACG GGTGCCCGGG TCCCCAGTCC CTCCGCCACG TGGGAAGCGC GGTCCTGGGC 361 GTCTGTGCCC GCGAATCCAC TGGGAGCCCG GCCTGGCCCC GACAGCGCAG CTGCTCCGGG 421 CGGACCCGGG GGTCTGGGCC GCGCTTCCCC GCCCGCGCGC CGCTCGCGCT CCCAGGGTGC 481 AGGGACGCCA GCGAGGGCCC CAGCGGAGAG AGGTCGAATC GGCCTAGGCT GTGGGGTAAC 541 CCGAGGGAGG GGCCATGATG TGGAGGCCCT GGGAACAGGT GCGTGCGGCG ACCCTTTGGC 601 CGCTGGCCTG ATCCGGAGAN CCAGGGCTGC CTCCAGGTCC GGACGCGGGG CGTCNGGCTC 661 CGGGCACCAC GAATGCCGGA CGTGAANGGG ANGNNGGANG CNCNNANNNN NNNNTGGGNN 721 NNNNNCNNNN NNNNNN //
Primer: pGL4pA_R (Pr0664) Region: SV40 pA,Luc 3' Sequence file: RDB20026_B3Gec.seq
>07379_20026_B3Ge_1_pGL4pA_R_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TNNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT 61 AAAAAACCTC CNNNNNNNNC NCNNNNACCT GNAACATAAA ATGAATGCAA TTGNNNNNNN 121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT 301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG 361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG 421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA 481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG 541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT 601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT 661 CCCAGTANGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG 721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC 781 GCTGGTTCAC ACCCAGTGTC TTANCGGTGT CNNAGTCCAC CACCTTANCC TCNAANAANN 841 NNNNCACCTT NCCTACTGCG CCNNGNTTGT CGNCCCCTNN NGGGGNGATC AGAANNGNNC 901 NNNNNGNTTC TGTNNNNNNG TNNNNNNTGG NNNNNGNCNG GNANNTNNNA NNCNNTTNNN 961 NNCNNNNNNN NNN //