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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phNOS2

Promoter collection, Human NOS2 promoter

Catalog number RDB07377
Resource name pGL4-phNOS2
Alternative name U2727(2105)-3
Clone info. PCR amplified human NOS2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 27801702 to 27800399(NC_000017.11); relatively -1173 to +131, where +1 corresponds to 1 nt of NM_000625.4.
Comment PCR cloning, forward primer: CGGTGAGATCAAGGGTGACT; reverse primer: GCCTTGAGAACTTCGGGACT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human nitric oxide synthase 2, inducible genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human nitric oxide synthase 2, inducible

          Reference sequence
            

          Distribution information

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          RDB07377 pGL4-phNOS2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07377.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12747_A4D2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12747_A4D2a.seq check
          >07377_12747_A4D2_pGL4-4174F_H11_23.ab1
              1 AGGTTCCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 CGGTGAGATC AAGGGTGACT TTTTTTCCCT TTGCTTCTCA ACTTCTCCCT AATGTAGTTG
            121 TTATTTTACT TTTATGATGG AAAAATGCAT ATGTATGGGA ATACTGTATT TCAGGCATTA
            181 TAAGGAATGA AATTATAGGC CGGGCATTGT GGCTAACCCT TGTAATCCTA GCACTTTGAG
            241 AGGCTGAAGT GGGCAGATCA CTTGAGCTTC AGAGTTCGAG ACCAGCATGG ACAACATGGT
            301 GAAACCCAGT CTCTACCAAA AACACAAAAA TATTAGCTGG GTGTGGTGGT GCATGCCTGT
            361 AGTCCCAGCT ACTCAGGAGG CTGAGGTGGG AGGATCGCTT GAGCCTGGGA GGCAGAAGTT
            421 GCAATGAGCA GAGATCGTGC CACTCCGCTC CAGTCTTGGT GACAGAATGA GACTCCATCT
            481 CAAAAATAAA TAAATAAATA AATAAAATAA ATGAAATGAA ATTATAAGAA ATTACCACTT
            541 TTTCATGTAA GAAGTGATCA TTTCCATTAT AAGGGAAGGA ATTTAATCCT ACCTGCCATT
            601 CCACCAAAGC TTACCTAGTG CTAAAGGATG AGGTGTTAGT AAGACCAACA TCTCAGAGGC
            661 CTCTCTGTGC CAATAGCCTT CCTTCCTTTC CCTTCCAAAA ACCTCAAGTG ACTAGTTCAG
            721 AGGCCTGTCT GGAATAATGG CATCATCTAA TATCACTGGC CTTCTGGAAC CTGGGCATTT
            781 TCCAGTGTGT TCCATACTGT CAATATTCCC CCAGCTTCCT GGACTCCTGT CACAAGCTGG
            841 AAAAGTGAGA GGATGGACAG GGATTAACCA GAGAGCTCCC TGCTGAGGAA AAAAATCTCC
            901 CAGATGCTGA AAGTGAGGCC ATGTGGCTTG GCCAAATAAA ACCTGGCTCC GTGGTGCCTC
            961 TGTCTTAGCA GCCACCCTGC TGATGAAACT GCCACCCTTG GACTTGGGAC CAGAAAGAGT
           1021 GGTTTGGGTG AAGAGGCACC ACACAGAGTG GATGTTAACC AGCAAGGATC AAGGTCACCT
          1081 CACAGACCTT GCCCAGTCAC AAGTCATAAA TTAGGCCTTA
          //
          Primer:
          Sequence file: RDB12747_A4D2b.seq check
          >07377_12747_A4D2_pGL4-136R_B11_05.ab1
              1 AGCTTTATGG GTTTTTGGGG CATCTTCCAT GGGTGGCTTT ACCAACAGTA CCGGAATTGC
             61 CAAGCTTGGC CGCCGAGGCC CATTATCTTG ATGCCTTGAG AACTTCGGGA CTGTCTAGAA
            121 CTGCCCAGTC CCCTCATCAA AGGTGGCCGA GAGATTTTAA AGCAGGAATG AGGCTGAGTT
            181 CTCTGGGGCC GGAGCCTCAG TTTTCGACTC GCTACAAAGT TATGAACACA CTGGCAGCCA
            241 AGAAGTATTT ATACTCACCC CATGCCATCC AGAGAGTTGT TTTTGCATAA AGGTCTCTTT
            301 TTTTTTTTTT TTTTTGGTTT CCAAAGGGAG TGTCCCCAGC TTGTGTACAG TTAGCTAATT
            361 TATGACTGTG ACTGCCAGGG CCTGTGGGTG ACCTGATCTT GCTGTTACAT CACTCTGTGT
            421 GGTGCCTCTT CACCCAACCC ACCTCTTTCT GGTCCCAAGT CCAAGGTGGC AGTTCATCAG
            481 CAGGGTGGCT GCTAAGACAG AGGCACCACG GAGCCAGGTT TTATTTGGCC AAGCCACATG
            541 GCCTCACTTT CAGCATCTGG GAGATTTTTT CCTCAACAGG GAGCTCTCTG GTTAATCCCT
            601 GTCCATCCTC TCACTTTTCC AGCTTGTGAC AGGAGTCCAG GAAGCTGGGG GAATATTGAC
            661 AGTATGGAAC ACACTGGAAA ATGCCCAGGT TCCAGAAGGC CAGTGATATT AGATGATGCC
            721 ATTATTCCAG ACAGGCCTCT GAACTAGTCA CTTGAGGTTT TTGGAAGGGA AAGGAAGGAA
            781 AGGCTATTGG CACAGAGAGG CCTCTGAGAT GTTGGTCTTA CTAACACCTC ATCCTTTAGC
            841 ACTAGGTAAG CTTTGGTGGA ATGGCAGGTA GGAATAAATT CCTTCCCTTA TAAATGGAAA
            901 ATGATCACCT TCTTACATGA AAAAAGTGGG AAATTTCCTT ATAAATTTCA TTTCCATTTA
            961 ATTTTATTTA TTTATTTAAT TTATTTTTGA GATGGGAGTC TTCAATTCTT GTTCACCCAA
           1021 GAACTGGGAG CCGGAGTGGG CACGAATCTC TGCTCATTGC AACCTTTCTT GCCTCCAAGG
           1081 CTCAAGCGAA TCCTTCCCCA CCCTTCAAGC CTCCCTTGGA ATAAGCTGGG GACCTTACAA
           1141 GGCAATGCAC CCACCACACC CAAGCCTAAT TATTTTTTCG TTGGTTTTTT TGGATATGAG
          1201 AAAGACAGCC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl