RDB07377 - pGL4-phNOS2

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pGL4-phNOS2 (#RDB07377)

Promoter collection, Human NOS2 promoter

Alternative name	U2727(2105)-3
Clone info.	PCR amplified human NOS2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 27801702 to 27800399(NC_000017.11); relatively -1173 to +131, where +1 corresponds to 1 nt of NM_000625.4.
Comment	PCR cloning, forward primer: CGGTGAGATCAAGGGTGACT; reverse primer: GCCTTGAGAACTTCGGGACT. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human nitric oxide synthase 2, inducible genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07377.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07377	pGL4-phNOS2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phNOS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07377).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12747_A4D2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB12747_A4D2a.seq
>07377_12747_A4D2_pGL4-4174F_H11_23.ab1 1 AGGTTCCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT 61 CGGTGAGATC AAGGGTGACT TTTTTTCCCT TTGCTTCTCA ACTTCTCCCT AATGTAGTTG 121 TTATTTTACT TTTATGATGG AAAAATGCAT ATGTATGGGA ATACTGTATT TCAGGCATTA 181 TAAGGAATGA AATTATAGGC CGGGCATTGT GGCTAACCCT TGTAATCCTA GCACTTTGAG 241 AGGCTGAAGT GGGCAGATCA CTTGAGCTTC AGAGTTCGAG ACCAGCATGG ACAACATGGT 301 GAAACCCAGT CTCTACCAAA AACACAAAAA TATTAGCTGG GTGTGGTGGT GCATGCCTGT 361 AGTCCCAGCT ACTCAGGAGG CTGAGGTGGG AGGATCGCTT GAGCCTGGGA GGCAGAAGTT 421 GCAATGAGCA GAGATCGTGC CACTCCGCTC CAGTCTTGGT GACAGAATGA GACTCCATCT 481 CAAAAATAAA TAAATAAATA AATAAAATAA ATGAAATGAA ATTATAAGAA ATTACCACTT 541 TTTCATGTAA GAAGTGATCA TTTCCATTAT AAGGGAAGGA ATTTAATCCT ACCTGCCATT 601 CCACCAAAGC TTACCTAGTG CTAAAGGATG AGGTGTTAGT AAGACCAACA TCTCAGAGGC 661 CTCTCTGTGC CAATAGCCTT CCTTCCTTTC CCTTCCAAAA ACCTCAAGTG ACTAGTTCAG 721 AGGCCTGTCT GGAATAATGG CATCATCTAA TATCACTGGC CTTCTGGAAC CTGGGCATTT 781 TCCAGTGTGT TCCATACTGT CAATATTCCC CCAGCTTCCT GGACTCCTGT CACAAGCTGG 841 AAAAGTGAGA GGATGGACAG GGATTAACCA GAGAGCTCCC TGCTGAGGAA AAAAATCTCC 901 CAGATGCTGA AAGTGAGGCC ATGTGGCTTG GCCAAATAAA ACCTGGCTCC GTGGTGCCTC 961 TGTCTTAGCA GCCACCCTGC TGATGAAACT GCCACCCTTG GACTTGGGAC CAGAAAGAGT 1021 GGTTTGGGTG AAGAGGCACC ACACAGAGTG GATGTTAACC AGCAAGGATC AAGGTCACCT 1081 CACAGACCTT GCCCAGTCAC AAGTCATAAA TTAGGCCTTA //
Primer: Sequence file: RDB12747_A4D2b.seq
>07377_12747_A4D2_pGL4-136R_B11_05.ab1 1 AGCTTTATGG GTTTTTGGGG CATCTTCCAT GGGTGGCTTT ACCAACAGTA CCGGAATTGC 61 CAAGCTTGGC CGCCGAGGCC CATTATCTTG ATGCCTTGAG AACTTCGGGA CTGTCTAGAA 121 CTGCCCAGTC CCCTCATCAA AGGTGGCCGA GAGATTTTAA AGCAGGAATG AGGCTGAGTT 181 CTCTGGGGCC GGAGCCTCAG TTTTCGACTC GCTACAAAGT TATGAACACA CTGGCAGCCA 241 AGAAGTATTT ATACTCACCC CATGCCATCC AGAGAGTTGT TTTTGCATAA AGGTCTCTTT 301 TTTTTTTTTT TTTTTGGTTT CCAAAGGGAG TGTCCCCAGC TTGTGTACAG TTAGCTAATT 361 TATGACTGTG ACTGCCAGGG CCTGTGGGTG ACCTGATCTT GCTGTTACAT CACTCTGTGT 421 GGTGCCTCTT CACCCAACCC ACCTCTTTCT GGTCCCAAGT CCAAGGTGGC AGTTCATCAG 481 CAGGGTGGCT GCTAAGACAG AGGCACCACG GAGCCAGGTT TTATTTGGCC AAGCCACATG 541 GCCTCACTTT CAGCATCTGG GAGATTTTTT CCTCAACAGG GAGCTCTCTG GTTAATCCCT 601 GTCCATCCTC TCACTTTTCC AGCTTGTGAC AGGAGTCCAG GAAGCTGGGG GAATATTGAC 661 AGTATGGAAC ACACTGGAAA ATGCCCAGGT TCCAGAAGGC CAGTGATATT AGATGATGCC 721 ATTATTCCAG ACAGGCCTCT GAACTAGTCA CTTGAGGTTT TTGGAAGGGA AAGGAAGGAA 781 AGGCTATTGG CACAGAGAGG CCTCTGAGAT GTTGGTCTTA CTAACACCTC ATCCTTTAGC 841 ACTAGGTAAG CTTTGGTGGA ATGGCAGGTA GGAATAAATT CCTTCCCTTA TAAATGGAAA 901 ATGATCACCT TCTTACATGA AAAAAGTGGG AAATTTCCTT ATAAATTTCA TTTCCATTTA 961 ATTTTATTTA TTTATTTAAT TTATTTTTGA GATGGGAGTC TTCAATTCTT GTTCACCCAA 1021 GAACTGGGAG CCGGAGTGGG CACGAATCTC TGCTCATTGC AACCTTTCTT GCCTCCAAGG 1081 CTCAAGCGAA TCCTTCCCCA CCCTTCAAGC CTCCCTTGGA ATAAGCTGGG GACCTTACAA 1141 GGCAATGCAC CCACCACACC CAAGCCTAAT TATTTTTTCG TTGGTTTTTT TGGATATGAG 1201 AAAGACAGCC T //