RDB07367 - pGL4-phEPCAM(TACSTD1)

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pGL4-phEPCAM(TACSTD1) (#RDB07367)

Promoter collection, Human EPCAM promoter

Alternative name	U2694(2072)-20
Clone info.	PCR amplified human EPCAM promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 47368067 to 47369577(NC_000002.12); relatively -1081 to +430, where +1 corresponds to 1 nt of NM_002354.2.
Comment	PCR cloning, forward primer: CCAGCCTCCTTTCCTCTTTT; reverse primer: CTGAGCTGCGGCAAAAGTC. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human tumor-associated calcium signal transducer 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07367.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07367	pGL4-phEPCAM(TACSTD1)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phEPCAM(TACSTD1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07367).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07367_A3L2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB07367_A3L2a.seq
>07367_07367_A3L2_pGL4-4174F_A06_03.ab1 1 ACAGGGGGCA GGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA 61 GGATCCAGCC TCCTTTCCTC TTTTTAACTC TTACTTTTAT GATTTCTTTA GTGGATAAAA 121 AGCTTTTAAA AAATAGGTTA CAATGATATT ACAGCTAACA AAAAATAACA TTTAAAAACA 181 CTAAATAGTA TATATATGAA GTATTTATAA TTATTTTAAT ATTGTAATAA TATAGTGTGT 241 TGTGATTTGA ATTCATCTGC ACGGAAATCG ATTACTGTCC TTTCTTTCTA TTTCCCTATA 301 TTTTCTTTCC GAAGGCGTCA TCAACATTTT GGTTCTTTAA TAGTAACCAA AACCCGAAAT 361 CATCTCGGTT CTCAGTATTT GGCTCTATGG GAACACCTTT TCTTTTCTCT CTTTTTTTTT 421 TTTTTGAGAC GGAGTCTTGC TCCTGTCGCC CAGGCTGGAG TGTAATGGCA CGATCTCTGC 481 TCACTGCAAC CTCAGCCTCC CCAGTAGCTG GGATTACAGG CATGCGCCAC CACGCCCGGC 541 TAATTTTGTA TCTTTTAGTA GAGACGGCGT TCCTCCATGT TGGTCAGGCT GGTCTCGAAC 601 TTCAAACCTC AGGTGATCCG CCCGCCTCGG CCTCCCAAAG TGCTAGGATT ACAGGCGTGA 661 GCCACCGCGC TCAGCCTGGG AACACCTTTT CTTACATCTT //
Primer: Sequence file: RDB07367_A3L2b.seq
>07367_07367_A3L2_pGL4-136R_B06_06.ab1 1 GCCTTTAACG TTTTGGCATC TGCCATGGGG GCTTTACCAA GCAGTACCGG ATTGCCAAGC 61 TTGGCCGCCG AGGCCAGATC TTGATCTGAG CTGCGGCAAA AGTCGCCGTC GCCGCGGCAA 121 GCAGAAGCCC GAACGCGAGG ACCTGCGGGG GCGCCATGCT GCGCGCGCGC CGAGAAGAGG 181 GGCGCGGGAG GGGCCCGGGA CTCGCGGGAG GGCGTGCGCC GGGAGTGGGA CACGAGGAGC 241 CGGCCGGGCA GCGCGAGGCC TGGGGCACGC GGGTCCGCGT CGGGAGGACA GCGACGAGGG 301 GGTCCCCGGA CCGCGTCGAA GGTGCTCGCT CGCCGAAGGA CTAGCGCTCT GTGCGCGCGG 361 CGGGGCGGAG CGCACACCTG CCCGACCTGG CGGCCCCGCC TCTCGGCCCC TTCCCGGGAC 421 TGCTCACCTC TGGCCGCTGG TGCTCGTTGA TGAGTCACCG CCCGGGAGTT GGGGGAGTGA 481 GTAGGCTCCC CTCCCCCAGC CCCGGCGCTG CAGTTGGGGC GCGGCCCTCT CCACAGGTGT 541 GGGCCCGCGC GCCCCGCTCC GCCCCGCCCC AGGTGCTTCG TCTCCATGGC GGCGTTAGGG 601 ATCTTTGGTG GCCTCCGAGG AGGTTTGAAG ATTCTGTGCT GAGACTTCCT TTTAACCGGA 661 GGAGCGCTGC TCTGAAGCCT TGAAGAATGG GGCCTGGGGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

human tumor-associated calcium signal transducer 1

External Database
Human tumor-associated calcium signal transducer 1

Reference sequence