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pGL4-phRB1 (#RDB07345)

Promoter collection, Human RB1 promoter

Alternative name U2734(2112)-3
Clone info. PCR amplified human RB1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 48302465 to 48303797(NC_000013.11); relatively -1282 to +51, where +1 corresponds to 1 nt of NM_000321.2.
Comment PCR cloning, forward primer: GACGAGTTCAGATGGGCAAT; reverse primer: AACGTCCCCTGAGAAAAACC. Genomic fragment 48,302,464 to 48,303,797 of NC_000013.11 is cloned. Known difference: deletion of 5 nt of 48,302,963 to 48,302,967; T to G substitution at 48,303,180.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human retinoblastoma 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07345.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07345 pGL4-phRB1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phRB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07345).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07345_A5Gu.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07345_A5Gua.seq check
>07345_07345_A5Gu_pGL4-4174F_C04_07.ab1
    1 ACTGGGGCAG GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATGACGAGTT CAGATGGGCA ATAACAGTGT CTGCCAAAGA GAAAAAAAAA TGTATTCAAA
  121 CTAGATAATC TATTGGTACA AATACCGAGA CACAGAAGTG ATAACAGCTT TAAGCCAATG
  181 TTTGATGGTG GTAGTCCCAG CAAGCTCTTT TCTGATGTCT TTGTGCCTTT GCACATGCTC
  241 CTTCTCTGTC ACTGTTTTCT TCATCAAACA TAATATAATG GACAAGTGGA ATCAAATAGA
  301 ATTGAGTTCA AATTCTCTGC TACCCATCGG CCCTGGTATT GGACAAATTA ACTCCTCTGA
  361 GCCTGTTTCC TCATCTGCAA CGTAGACTAG CTAATACTAC CCATTGGAAA GCGTTGTTTC
  421 TTAGCTAATG CATGCAAGGC TTAAAACCTA GATGACGGGT TGATAGGTGC AGCAAACCTC
  481 CATGGCATAC GTATGCCTAT GTAACAAACC TACACGTTCT GCACTTGTAT CCCGGAACTT
  541 AAAGTAAAAA AAAAAAAAAA AAAAGAAAGA AAGAAAAAGA AAAAAAAGGC TGTTTCTGGG
  601 GATTAAATAA GACAATTATG TAAGGTGGCC AGCACAGTTC CTGGTACATA GTAAATGTCA
  661 GGCCTGCCTG ACAGACTTCT ATTCAGCAGC TACTGCTCCC CTGAAAATCT TCCTCAGACG
  721 TTTCCACGGT GCTTCCCGTT CTTACACCAC TACAATCCTT TATTACACTA CTCTCCGTTC
  781 ATTCCCCACA GCTCCCTCCC TTCCTTTCCC TAACCAGTGA TCCCAAAAGG CCAGCAAGTG
  841 TCTAACATTT TCTATCTTCT AAGTGACTGG TAAAGTTCCG CACCTATCAG CGCTCCAAGT
  901 TTGTTTTTGT TTTGGCCGAC TTTGCAAAAC GGATTGGGCG GGATGAGAGG TGGGGGGCGC
  961 CGCCCAAAGG AGGGAGAGTG GCGCTCCCGC CGAGGGTGCA CTAGCCAGAT ATTCCCTGCG
 1021 GGGCCCGAGA GTCTTCCCTA TCAGACCCCG GGATAGGATG AGCCCACAGT CACCCCACCA
 1081 GACTCTTGTA TAGCCCCGAT TAAGTGCACC CGACCTTGCA AGGGGTGGCT CTGGCTAGGA
1141 GGCACCGTCC GGGGCTCG
//
Primer: pGL4-136R
Sequence file: RDB07345_A5Gub.seq check
>07345_07345_A5Gu_pGL4-136R_D04_10.ab1
    1 GCTATGTTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
   61 CGCCGAGGCC AGATCTTGAT AACGTCCCCT GAGAAAAACC GGACGCGCCC TCCCCCGCCC
  121 GGCAACTGAG CGCCGCGTCC AACCGCGGGA AAACGTCACT TCCGCCCGCG GCGTCACGTC
  181 CGCGAGGCTC CCGGGCCCGC CGGCGTCTGT GGGGAACTGG GGCGCTGGTC GGTGCGCGGG
  241 CTGGGACGCT AAGTCATGAG GAATTAAACT GGGAAACCTG GCGTGGGTCC AGGCGCCTTC
  301 CAGGAGGCAT CCGGCGCGGC CCGGACGTGC TTCTACCCAG AACCACCCCC TCCAGGCCGG
  361 GGTGCACTTA ACGGGGCTAT ACAAAGAGTC TGGTGGGTGA CTGTGGGCCT CATCCCTATC
  421 CCGGGGTCTG ATAGGGAAGA CTCTCGGGCC CCGCAGGGAA TATCTGGCTA GTGCACCCTC
  481 GGCGGGAGCG CCACTCTCCC TCCTTGGGCG GCGCCCCCCA CCTCTCATCC CGCCCAATCC
  541 GTTTTGCAAA GTCGGCCAAA ACAAAAACAA ACTTGGAGCG CTGATAGGTG CGGAACTTTA
  601 CCAGTCACTT AGAAGATAGA AAATGTTAGA CACTTGCTGG CCTTTTGGGA TCACTGGTTA
  661 GGGAAAGGAA GGGAGGGAGC TGTGGGGAAT GAACGGAGAG TAGTGTAATA AAGGATTGTA
  721 GTGGTGTAAG AACGGGAAGC ACCGTGGAAA CGTCTGAGGA AGATTTTCAG GGGAGCAGTA
  781 GCTGCTGAAT AGAAGTCTGT CAGGCAGGCC TGACATTTAC TATGTACCAG GAACTGTGCT
  841 GGCCACCTTA CATAATTGTC TTATTTAATC CCCAGAACAG CCTTTTTTTT CTTTTTCTTT
  901 CTTTCTTTTT TTTTTTTTTT TTTTACTTTA AGTTCCGGGA TACAAGTGCA GAACGTGTAG
  961 GTTTGTTACA TAGGCATACG TATGCCATGG AAGGTTTGCT GCACCTATCA ACCCGTCATC
 1021 TAAGGTTTTA AAGCCCTTGC ATGCATTAGC TAAGAAAACA ACGCTTTCCC AAATTGGGGT
 1081 AGGTATTAGC CTTAGTCTTA CGGTGGCAGA TGAAGCAAAC AGGCTCAGAG GAGTTTAATT
1141 TGGTCCAAAT ACCCAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles