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pGL4-phPOLD1 (#RDB07344)

Promoter collection, Human POLD1 promoter

Alternative name U2732(2110)-6
Clone info. PCR amplified human POLD1 promoter sequence was inserted into a firefly luciferase vector. Genomic DNA (50,383,082 - 50,384,389nt of NC_000019.10) corresponding to 1 to 67nt of NM_001256849.1 and 1.2 kb up-stream was cloned.
Comment PCR cloning, forward primer: CTGATTGAGCAGGTGGGACT; reverse primer: CGCTTCAAACAGCGTTTCC. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human polymerase (DNA directed), delta 1, catalytic subunit 125kDa genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07344.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07344 pGL4-phPOLD1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPOLD1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07344).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07344_A8K8p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: pause site, insert 5'
Sequence file: RDB07344_A8K8a.seq check
>07344_07344_A8K8_pGL4-4174F_A02_02_ABI24.ab1
    1 TGGGGGTCCA GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
   61 GATCTGATTG AGCAGGTGGG ACTGGGTGTC CCCAGGCTGT TGGGAAAAGG GTGGCACATA
  121 GGGTCCCAGT GTTGGGTGGT GTGTCGCCTG CTGTGAAAGT TCAGGACAGA GGCCTCAGCC
  181 TCAGGGTCAC CTCCCTGGAC AAGGTGAATC AGAACTCACC CTTGAAGGAT AAACAGGATT
  241 TAAGTGAATG AGGGGACCTG GACTTCTGTC CGAACAACAA GTGTTTGCTG AGGCCTCCTG
  301 TGGGCCAGGC ACTGGGTTGG CCATTCTAGA GATGGAGACA TTCACCCAAT AAATGTTTCC
  361 AGAGCATTAA CTACAGTTAG GCACAATTCT AGGCACTGGA AACATCTGTG AACAAGATAA
  421 CCATCCTCCT ACCAAGACTC GGGGGATAGG GACTTTAATG AGCGTGGCAC TGTGGTGGGG
  481 CTAGAGGAAA TCAGGGAAGG TATCACAACA GAGGCAATCG CTGGGACCTA AAACAAGAGC
  541 CTGGGGGCTG GGCCTCCAAG TTGAGGGAAC AGCACGTGCA AAGTTTGAAA GGAGAGACGC
  601 CAGGAGTTCG GAGCGAAGCG ACGAAGTTCC TCCAATCCTT CCTGCCCCTG TGCCAGCCCT
  661 GAAGGAGCTG AATGAGCAAT TCCAATCGAG GGCGAAAGGG GGGTTGGATG GAGGGATGCA
  721 ACTTAAAGGT GTATTTATTT GGGGCGGGGA ACAGCGGAAG TGAGAGTAGT TACCAATGAT
  781 CACCTTATCC TAAAGAGTAG TGTACCGGGC GCCCCACAAG TGTCCCTATC ATGCGTTTGT
  841 TGAGTCTAAT TTACGTATAG GGAAACTGAA AGCTTAGGAA TGAAGGGAGA AACAGATCAA
  901 GGCCCAGGAA CTCTTACCCG CTTTATCGGG CCAAGCCCTC TTGCGGGGGA TCTTTGGGCG
  961 TCAGGGGTCA CCAAGCTTGG CCTGCCTGGG GCAAGCGGAC CGGGCCAAGC TGCTTCCTCT
 1021 TACAACGGCC CCCGGGGCGG AAGTAGGGTC GGGGGCCGGG GGGGGGGAAC GCGAAAGGGA
 1081 TTGCCCGCTG TCGCAAAACT AAACCTCGAG GGTTGGGAAG GGAAGAAAGA AAGTAGAAAA
1141 CTAAGGAATG CTCGTGCGTG CCGGTCGGCT CCCAGA
//
Primer: pGL4-136R (Pr0079)
Region: Luc 5', inset 3'
Sequence file: RDB07344_A8K8b.seq check
>07344_07344_A8K8_pGL4-136R_B02_05_ABI24.ab1
    1 GCCCTTAGTT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
   61 GGCCGCCGAG GCCAGATCTT GATCGCTTCA AACAGCGTTT CCCGCCACAG CCTACGCCGC
  121 CGTGACCCCT GACTCCCGCG CGCGCAAGCC CAGAGCCGCG GCGCTCAGAG ACGGGCCACG
  181 CCCCCGCCTG CCCAAGTGCG GGCAAGGCCA CGCCCCGCTT GTTCGACTGC AGGGCAGGCC
  241 CCGCCCCCTC TCTCAGCTTC CGAAGTGGCC TCGCCCCCTT GCTGAAAAAT TCCCTTAACT
  301 CCGCCTCCCC ATGACCTGTC TGCCCGAAGC CACGCCCCTC CGCGCGGCTA CCTTCACGAC
  361 CTCGTGCGGT CTGGGCGCGC CGCGGTTCTG TTCTCTCTCC TCCCACCCTC GGGTGTTGCG
  421 CGCGCATCCT CCGCGTCCCC CCGCCCGCCT CTCGCCCGGG GCCGTGTAGG GAGCGCTGCC
  481 CCGTCGCTGC CCAGCAGCCA GCTGGTGACC TGACGCCCAA GATCCCCGCA GAGGCTGGCC
  541 GATAAGCGGT AGAGTCCTGG CTTGATCTGT TCTCCTTCAT CCTAGCTTCA GTTTCCCTAT
  601 ACGTAAATTA GACTCAACAA CGCATGATAG GGACACTTGT GGGGCGCCCG GTACACTACT
  661 CTTAGGATAA GGTGATCATT GGTAACTACT CTCACTTCCG CTGTTCCCCG CCCCAAATAA
  721 ATACACCTTT AAGTTGCATC CCTCCATCCA ACCCCCCTTT CGCCCTCGAT TGGAATTGCT
  781 CATTCAGCTC CTTCAGGGCT GGCACAGGGG CAGGAAGGAT TGGAGGAACT TCGTCGCTTC
  841 GCTCCGAACT CCTGGCGTCT CTCCTTTCAA CTTTGCACGT GCTGTTCCCT CAACTTGGAG
  901 GCCCAGCCCC CCAGGCTCTT GTTTTAGGTC CCAGCGATTG CCTCTGTTGT GATACCTTCC
  961 CTGATTTCCT CTAGCCCCAC CCACAGTGCC ACGCTCATTT AAGTTCCCTA TCCCCCGAGT
 1021 TCTTGTAGGA AGATGGTTTA TCTGTCACAG ATGGTTCCAG TGCCTAGATG TGCCCTTAAC
1081 CTGTAGTTAA TGCCTCTTGG AAAAAC
//
Primer: pAxCALNLF1 (Pr0166)
Region: Luc 3'
Sequence file: RDB07344_A8K8c.seq check
>07344_07344_A8K8_pAxCALNLF1_C02_08_ABI24.ab1
    1 TCAAGGGTAT CTTATCATTG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
   61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
  121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
  181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
  241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
  301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
  361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
  421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
  481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
  541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
  601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
  661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
  721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
  781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
  841 GTGTGGGGCA CCAGCAGGGC AGATTGAATC TTATAGTCTT GCAAGCTGCG CAAGAATAGC
  901 TCCTCCTCGA AGCGGTACAT GAGCACGACC CGAAAGCCGC AGATCAAGTA GCCCAGCGTG
  961 GTGAACATGC CGAAAGCCGG TGGTGAAATG GCACCCACGC TGAGGATAGC GGTGGTCGGG
 1021 GGATGGATCT GGTTGCCCGG AAGATTGGGG TCGCGGGGCC ATGACTGAAT CGGACACAAA
1081 GCGCTGCCGG TTGCCGGATA GGGGCCTTAA CGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles