Promoter collection, Human PLK1 promoter
Catalog number | RDB07327 |
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Resource name | pGL4-phPLK1 |
Alternative name | U2688(2066)-2 |
Clone info. | PCR amplified human PLK1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 23677730 to 23678950(NC_000016.10); relatively -1042 to +179, where +1 corresponds to 1 nt of NM_005030.6. |
Comment | PCR cloning, forward primer: AGGGTTGCCACTCAAGTACC; reverse primer: AGTCACTGCAGCACTCATGC. Entire sequence of promoter region has not been confirmed. |
Vector backbone | pGL4.10 [Luc2] (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Human polo-like kinase 1 genomic DNA |
Depositor|Developer | DNA Bank, | |
Sequence (full) | RDB07327hts01.seq assembled from experimentally sequenced data |
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Remarks, protocol and/or map (pdf) | RDB07327.pdf |
Test sheet | RDB20129_B3K9p1-1.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
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Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07327 | pGL4-phPLK1 | DNA solution |
Materials & Methods section:
The pGL4-phPLK1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07327). |
Reference section:
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