Resource data sheet
DNA Bank Top

pGL4-phKLK3 (#RDB07324)

Promoter collection, Human KLK3 promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB07324.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name U2682(2060)-1
Clone info. PCR amplified human KLK3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 50853756 to 50854937(NC_000019.10); relatively -1159 to +23, where +1 corresponds to 1 nt of NM_001648.2.
Comment PCR cloning, forward primer: CCTGACCAACATGGTGAAACT; reverse primer: GTGCAGGTGGTAAGCTTGG. Differences compared with NC_000019.10 : 50853989 - 50853990 ins T, 50854248 C > T, 50854664 G > A, 50854710 - 50854711 ins A.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Human kallikrein-related peptidase 3 genomic DNA
Depositor|Developer DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 謝辞の表明を必要とする。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB07324 pGL4-phKLK3 DNA solution

How to cite this biological resource

Materials & Methods section:

The pGL4-phKLK3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07324).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Lin, Y.C., Di-n-butyl phthalate promotes the neural differentiation of mouse embryonic stem cells through neurogenic differentiation 1. Environ. Pollut. 347: 123722 (2024). PMID 38460589. [PubMed] [Article] [RRC of NBRP]