RDB07324 - pGL4-phKLK3

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pGL4-phKLK3 (#RDB07324)

Promoter collection, Human KLK3 promoter

Alternative name	U2682(2060)-1
Clone info.	PCR amplified human KLK3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 50853756 to 50854937(NC_000019.10); relatively -1159 to +23, where +1 corresponds to 1 nt of NM_001648.2.
Comment	Genomic fragment 50,853,756 to 50,854,937 of NC_000019.10 is cloned. PCR cloning, forward primer: CCTGACCAACATGGTGAAACT; reverse primer: GTGCAGGTGGTAAGCTTGG. Known differences: insertion of T after 50,853,989; C to A substitution at 50,854,664; insertion of A after 50,854,710. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human kallikrein-related peptidase 3 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07324.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07324	pGL4-phKLK3	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phKLK3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07324).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07324_A6Jp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F () Sequence file: RDB07324_A6Jpa.seq
>07324_07324_A6Jp_pGL4-4174F_E05_05_ABI08.ab1 1 ATAGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TCCTGACCAA CATGGTGAAA CTCTGTCTCT ACTAAAAAAA AAAAAAATAG AAAAATTAGC 121 CGGGCGTGGT GGCACACGGC ACCTGTAATC CCAGCTACTG AGGAGGCTGA GGCAGGAGAA 181 TCACTTGAAC CCAGAAGGCA GAGGTTGCAA TGAGCCGAGA TTGCGCCACT GCACTCCAGC 241 CTGGGTGACA GAGTGAGACT CTGTCTCAAA AAAAAAAAAT TTTTTTTTTT TTTTTTGTAA 301 AGATGGATCT TGCTTTGTTT CTCTGGTTGG CCTTGAACTC CTGGCTTCAA GTGATCCTCC 361 TACCTTGGCC TCGGAAAGTG TTGGGATTAC AGGCGTGAGC CACCATGACT GACCTGTCGT 421 TTAATCTTGA GGTACATAAA CCTGGCTCCT AAAGGCTAAA TATTTTGTTG GAGAAGGGGC 481 ATTGGATTTT GCATGAGGAT GATTCTGACC TGGGAGGGCA GGTCAGCAGG CATCTCTGTT 541 GCACAGATAG AGTGTACAGG TCTGGAGAAC AAGGAGTGGG GGGTTATTGG AATTCCACAT 601 TGTTTGCTGC ACGTTGGATT TTGAAATGCT AGGGAACTTT GGGAGACTCA TATTTCTGGG 661 CTAGAGGATC TGTGGACCAC AAGATCTTTT TATGATGACA GTAGCAATGT ATCTGTGGAG 721 CTGGATTCTG GGTTGGGAGT GCAAGGAAAA GAATGTACTA AATGCCAAGA CATCTATTTC 781 AGGAGCATGA GGAATAAAAG TTCTAGTTTC TGGTCTCAGA GTGGTGCAGG GATCAGGGAG 841 TCTCACAATC TCCTGAGTGC TGGTGTCTTA GGGCACACTG GGTCTTGGAG TGCAAAGGAT 901 CTAGCACGTG AGGCTTTGTA TGAAGAAATC GGGGATCGTA CCCACCCCCC TGTTCTGTTT 961 CATCCTGGGC ATGTCTCTCT GCCTTTGTCC CCTAGATGAA GTCTCATGAG CTACAAGGCC 1021 TGGTGCATCA GGTGATCTAG TAATGCAGAC AGCAAGTGCC TAGCTCTCCT CCCCTTCAAG 1081 CTCTAGTGTG TAAGGATGTC CAGCCCTCCA GCAG //
Primer: pGL4-136R () Sequence file: RDB07324_A6Jpb.seq
>07324_07324_A6Jp_pGL4-136R_F05_06_ABI08.ab1 1 GCCTTTATGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATGTGCAG GTGGTAAGCT TGGGGCTGGG GAGCCTCCCC 121 CAGGAGCCCT ATAAAACCTT CATTCCCCAG GACTCCGCCC CTGCCCTGCT GGCACCCAGA 181 GGCTGACCAA GGCCCTCCCC ATGCTGCTGG AGGCTGGACA ACCCCCTCCC ACACCCAGAG 241 CTGTGGAAGG GGAGGGAGAG CTAGCACTTG CTGTTCTGCA ATTACTAGAT CACCCTGGAT 301 GCACCAGGCC CTTGTAGCTC ATGGAGACTT CATCTAGGGG ACAAAGGCAG AGGAGACATG 361 CCCAGGATGA AACAGAAACA GGGGGTGGGT ACGATCCCCG ATTCTTCATA CAAAGCCTCA 421 CGTGCCTAGA TCCTTTGCAC TCCAAGACCC AGTGTGCCCT AAGACACCAG CACTCAGGAG 481 ATTGTGAGAC TCCCTGATCC CTGCACCACT CTGAGACCAG AAACTAGAAC TTTTATTCCT 541 CATGCTCCTG AAATAGATGT CTTGGCATTT AGTACATTCT TTTCCTTGCA CTCCCAACCC 601 AGAATCCAGC TCCACAGATA CATTGCTACT GTCATCATAA AAAGATCTTG TGGTCCACAG 661 ATCCTCTAGC CCAGAAATAT GAGTCTCCCA AAGTTCCCTA GCATTTCAAA ATCCAACGTG 721 CAGCAAACAA TGTGGAATTC CAATAACCCC CCACTCCTTG TTCTCCAGAC CTGTACACTC 781 TATCTGTGCA ACAGAGATGC CTGCTGACCT GCCCTCCCAG GTCAGAAATC ATCCTCATGC 841 AAAATCCAAT GCCCCTTCTC CAACAAAATA TTTAGCCTTT AGGAGCCAGG TTTATGTACC 901 TCAAGATTAA ACGACAGGTC AGTCATGGTG GCTCACGCCC TGTAATCCCA ACACTTTCCC 961 GAGGCCCAAG GTAGGAGATC ACTTTGAAAG CCCAGGGAGT TTCAAGCCAA CCCAGAGATC 1021 AAGCAGATCC CAATCTCTAA CGAAAAGAGG AGATTTTTTT TTTGAGACGA GTCTTCATCT 1081 GTCACTAAGG CCTTGTATTG CAGTTGCGCC AATCCTCCCG GCTCA //
Primer: pAxCALNLF1 () Sequence file: RDB07324_A6Jpc.seq
>07324_07324_A6Jp_pAxCALNL_F1_G05_07_ABI08.ab1 1 AAATTTATCT TATCATGTCT GCTCGAAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA 61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA 121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT 181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC 241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG 301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG 361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT 421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG 481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA 541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT 601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA 661 GAATGGCGCT GGTTGTTTCT GTCACGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC 721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT 781 GCAAGTTGCT TAGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT 841 GGGGCACCAG CAAGGGCAGA TTGAAATCTT AATAGTCTTG CAAGCTGCGC AAGAAAAAAG 901 CTCCTCCCTC GAAGCGTTAC ATGAGCACGA ACCGAAAGCG GCAGAATCAA GTAGCCAAGC 961 GTGGTGAAAC AATGGCGGAA GCGTGGTTGA ATGGCACCAC GCTGAAGATA AGCGGTGTCC 1021 GGAATGAATC TGATGCCGAA ATGGGTCCGG CATGACTGAA TCGACACAAC CGTTGCGCGT 1081 C //