Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phIL6

Promoter collection, Human IL6 promoter

Catalog number RDB07313
Resource name pGL4-phIL6
Alternative name U2666 (2044)-7
Clone info. PCR amplified human IL6 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 22725956 to 22727254(NC_000007.14); relatively -1186 to +113, where +1 corresponds to 1 nt of NM_000600.4.
Comment PCR cloning, forward primer: agagctgtctgggtctctgg; reverse primer: tcctggaggggagatagagc. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human interleukin 6 (interferon, beta 2) genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
Other clones in our bank

External Database
Human interleukin 6 (interferon, beta 2)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 謝辞の表明を必要とする。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB07313 pGL4-phIL6 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07313.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0043e (English text)
          Featured content Dnaconda's recommendation EXR0043j (Japanese text)
          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07313_A3F6p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07313_A3F6a_2.seq check
          >07313_07313_A3F6_pGL4-4174F_G07_19.ab1
              1 AATAGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
             61 TAGAGCTGTC TGGGTCTCTG GAGACTGGAG GGACAACCTA GTCTAGAGCC CATTTGCATG
            121 AGACCAAGGA TCCTCCTGCA AGAGACACCA TCCTGAGGGA AGAGGGCTTC TGAACCAGCT
            181 TGACCCAATA AGAAATTCTT GGGTGCCGAC GCGGAAGCAG ATTCAGAGCC TAGAGCCGTG
            241 CCTGCGTCCG TAGTTTCCTT CTAGCTTCTT TTGATTTCAA ATCAAGACTT ACAGGGAGAG
            301 GGAGCGATAA ACACAAACTC TGCAAGATGC CACAAGGTCC TCCTTTGACA TCCCCAACAA
            361 AGAGGTGAGT AGTATTCTCC CCCTTTCTGC CCTGAACCAA GTGGGCTTCA GTAATTTCAG
            421 GGCTCCAGGA GACCTGGGGC CCATGCAGGT GCCCCAGTGA AACAGTGGTG AAGAGACTCA
            481 GTGGCAATGG GGAGAGCACT GGCAGCACAA GGCAAACCTC TGGCACAGAG AGCAAAGTCC
            541 TCACTGGGAG GATTCCCAAG GGGTCACTTG GGAGAGGGCA GGGCAGCAGC CAACCTCCTC
            601 TAAGTGGGCT GAAGCAGGTG AAGAAAGTGG CAGAAGCCAC GCGGTGGCAA AAAGGAGTCA
            661 CACACTCCAC CTGGAGACGC CTTGAAGTAA CTGCACGAAA TTTGAGGATG GCCAGGCAGT
            721 TCTACAACAG CCGCTCACAG GGAGAGCCAG AACACAGAAG AACTCAGATG ACTGGTAGTA
            781 TTACCTTCTT CATAATCCCA GGCTTGGGGG GCTGCGATGG AGTCAGAGGA AACTCAGTTC
            841 AGAACATCTT TGGTTTTTAC AAATACAAAT TAACTGGAAC GCTAAATTCT AGCCTGTTAA
            901 TCTGGTCACT GAAAAAAAAT TTTTTTTTTT TCAAAAAACA TAGCTTTAGC TTATTTTTTT
            961 TCTCTTTGTA AAACTTCGTG CATGACTTCA GCTTTACTCT TTGTCAAGAC ATGCCCAAAG
           1021 TGCTGAGTCA CTATAAAAGA AAAAAAAAGA AAGTAAAAGA GAAGTGATCT GCTCTAGCGC
           1081 TAGCCTCATG ACCGAACTTA GCCTGCACTT TCTCTAGCTG TGTCTGCATG CTAAGGAACG
          1141 TTCACATGGC ACCAATCCTT AAATAAGGAT TTTTCA
          //
          Primer: pGL4-136R
          Sequence file: RDB07313_A3F6b_2.seq check
          >07313_07313_A3F6_pGL4-136R_H07_22.ab1
              1 GTTTCAAAGG GTTTTTTGGC AACCTTTCCA TGGTTGGCTT TACCAACAGT AACCGGATTG
             61 CCAAGCTTGG CCGCCGAGGC CAGATCTTGA TTCCTGGAGG GGAGATAGAG CTTCTCTTTC
            121 GTTCCCGGTG GGCTCGAGGG CAGAATGAGC CTCAGACATC TCCAGTCCTA TATTTATTGG
            181 GGGTTGAGAC TCTAATATTG AGACTCATGG GAAAATCCCA CATTTGATAA ATCTTTGTTG
            241 GAGGGTGAGG GTGGGGCCAG AGCGGGTGGG GCTGATTGGA AACCTTATTA AGATTGTGCA
            301 ATGTGACGTC CTTTAGCATG GCAAGACACA ACTAGGGGGA AAAGTGCAGC TTAGGTCGTC
            361 ATTGAGGCTA GCGCTAAGAA GCAGAACCAC TCTTCCTTTA CTTTCTTTTT TTCTTTTATT
            421 AGTGACTCAG CACTTTGGCA TGTCTTGACA AAGAGTAAAG CTGAAGTCAT GCACGAAGTT
            481 TTACAAAGAG AAAAAAAATA AGCTAAAGCT ATGTTTTTTG AAAAAAAAAA AATTTTTTTT
            541 CAGTGACCAG ATTAACAGGC TAGAATTTAG CGTTCCAGTT AATTTGTATT TGTAAAAACC
            601 AAAGATGTTC TGAACTGAGT TTCCTCTGAC TCCATCGCAG CCCCCCAAGC CTGGGATTAT
            661 GAAGAAGGTA ATACTACCAG TCATCTGAGT TCTTCTGTGT TCTGGCTCTC CCTGTGAGCG
            721 GCTGTTGTAG AACTGCCTGG CCATCCTCAA ATTTCGTGCA GTTACTTCAA GGCGTCTCCA
            781 GGTGGAGTGT GTGACTCCTT TTTGCCAACC GCGTGGCTTC TGCCACTTTC TTCACCTGCT
            841 TCAGCCCACT TAGAGGAGGT TGGCTGCTGC CCTGCCCTCT CCCAAGTGAA CCCCTTGGGG
            901 AATCCTCCCA GTGAGGGACT TTTGCTCTCT GTGCCCAGAG GTTTTGCCTT TGTGCTGCCC
            961 AGTGCCTCTC CCCATTGCCC ACTGAAGTCT CTTCACCACT GTTTTCACTG GGGGCACCCT
           1021 GGCATGGGGC CCCAGGTCTC CCTGGAAGCC CTGAAAATAC TGAAGCCACT TTGGTTCAGG
           1081 GCCAGAAAAG GGGGGAGAAT ACTTACTTCA CCCTTCTTTG TGGGGAATGT TCAAAGGAGG
           1141 GAACCTTGTG CATCTTGCAA AAGTTTGGTG ATTTATATCG CTCTCCCATC CTTCCCCTCT
          1201 GTATAAGTAC TCTGTGAATA TTGA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07313_A3F6c_2.seq check
          >07313_07313_A3F6_pAxCALNL-F1_D01_04_ABI08.ab1
              1 ACAGGGAATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
             61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
            121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
            181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
            241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
            301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
            361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
            421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
            481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC
            541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC
            601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC
            661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG
            721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG
            781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT
            841 GTGGGCACCA GCAGGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAAATAGCT
            901 CCTCCTCGAA GCGGTACATG AGCACGACCC GAAAGCCGCA GATCAAGTAG CCCAGCGTGG
            961 ATGAAACATG CCGAAGCCCG TGGGTGAAAT GGCACCACGG CTGAGGATAG CGGTGTCGGG
           1021 GGATGATCTG GTTTGGCCGA GGATGGGGTC GCGGACAATG ACTGATCGAA CAACAAGCCG
          1081 GATGCCGTTG CCGGTTAAGC CTAACGCCCG TTGGGCAAAA TTCGGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Zimmermann, M., Chromatin remodelling and autocrine TNF alpha are required for optimal interleukin-6 expression in activated human neutrophils. Nat. Commun. 6: 6061 (2015). PMID 25616107.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl