Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phPLAUR (uPAR)

Promoter collection, Human PLAUR promoter

Catalog number RDB07312
Resource name pGL4-phPLAUR (uPAR)
Alternative name U2665 (2043)-6
Clone info. PCR amplified human PLAUR promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 43671502 to 43670283(NC_000019.10); relatively -1157 to +63, where +1 corresponds to 1 nt of NM_002659.4.
Comment PCR cloning, forward primer: ttgagacagggtctcgctct; reverse primer: ctcctccgccacaaacttc. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human plasminogen activator, urokinase receptor genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
Other clones in our bank

External Database
Human plasminogen activator, urokinase receptor

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 謝辞の表明を必要とする。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB07312 pGL4-phPLAUR (uPAR) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07312.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19225_B1Hbp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site, insert 5'
          Sequence file: RDB19225_B1Hba.seq check
          >07312_19225_B1Hb_1_pGL4ins_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNCC NNGTGCAAGT GCAGGTGCCA GAACATTTCT CTGGCCTAAC
             61 TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG GATTTGAGAC AGGGTCTCGC TCTGTTGCCC
            121 AGGTTGGAGT GCAGTGGTGC AATCATAGCT CACTGCAGCT TCCAGTTCCC AGGTTCAAGC
            181 GGTCCTCCCA CCTTAGCCTC CCAACTACCT AGGACTACAA GTACGGGCCA CTGCACCCAG
            241 CTAATTTTAT TTTATTTTTA ATTTCTTTTG GAAAGATGAG GGCTCTTTAT GTTTCCCAAG
            301 CTGATCTTGA ACTCCTGGCC CCAAGTGATC CTCCTGCCTT GGCTTCCCAA AGCATTGGTG
            361 TTACAGGCAT GAGCCACCTC ATCTGACCTC TTCTAAAATA TTATAAAATA AATACAGACA
            421 TCAAAATGAA AACAAAAGAA AAAGAAAAAA CAAAACAAAA ATCCCCAAAA CAACAACAAC
            481 AAAAAATGCA GACATCAATA ACATTCACCA CTAAATACTC TAGTAANNNN NNGCAAAAAC
            541 NAACAAACAA ACAAAAACCA GAATCCCACC TAAGCATACN GCCATTGTCG NAACAGTGAT
          601 ATCNACTTTT TTGNNTGANG NGNNNNNNNN NN
          //
          Primer: pGL4ins_R
          Region: insert 3'
          Sequence file: RDB19225_B1Hbb.seq check
          >07312_19225_B1Hb_1_pGL4ns_R_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGCGCTGGGC CCTTCTTAAT GTTTTTGGCA TCTTCCNTGG
             61 TGGCTTTACC AACAGTACCG GATTGCCAAG CTTGGCCGCC GAGGCCAGAT CTTGATCTCC
            121 TCCGCCACAA ACTTCCCTTC CCGGGGCGCG GCCGGCTGGT GGTGAAGGGG CTGGCTCGGC
            181 CCTGACTCAT GGAGTTGTGA TCACAGCTCT GTCCCCAGAT TGCCTGGGTG CAAATCCCAG
            241 TTCTCTCTCT TCCTAACGTG GGACCCGGGG CAATCGCTCT CCACTGCTGT AAAATGAGGA
            301 TAAAAACTTT GACGGTAAAT ATGAATGTGG CTGATTATTT GGCTAACAGG ATTTTTGTTA
            361 GTTTTGTCAG GAGGGATACT GGTATTTTGG AAAATGTCCT TATCTAGAGA TGCATCCTGA
            421 AATATGCATC TCTTAAACAC TTAAAACACT TAACCCTTGC TTTGCTTCCC GCTGTGCCCC
            481 CAGCTCCTGG ACCAGTGCCC AGCACGAAGT AGGTCCTTGG TAAATGTTAA AGGAATGTGT
            541 TGAGCCCTCC ATTTTGGCCA GCAGCTATTC CAGGCATTGC ACCTACATTA AAACACTTAA
            601 TGNTGAAACC CCGTCTCNAC TAAAANNACA AAAANTNNNC CNGGCNTANN GNNGTGTGTG
            661 TNNTACTTAN NNNGCTGANN CNNNNAATNN CTTGNNNCNN GGANGCNNAN GNTGGGTTGN
          721 NNNGNNNNNA NNNNNNNCNN NNNNCNNCNN NNNNNNNN
          //
          Primer: SV40pA_R
          Region: SV40 pA,Luc 3'
          Sequence file: RDB19225_B1Hbc.seq check
          >07312_19225_B1Hb_1_SV40pA_R_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTTTTTTNNC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
             61 TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
            121 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
            181 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
            241 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
            301 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
            361 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
            421 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
            481 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
            541 GCGTTTGTAG CCTCGGGGTT GTTAACGTAN CCGCTCATGA TCATGGGGCC NCGGACGCNN
            601 AGCTCGNCNN NNNTGGTNNN NNNCNAGNGT CTTACCGGTG TCCNAGTCCA CCACNNTANC
          661 CTCNNNNAAN GGNNNCCNNC TTNNNANTGN NNNAGGNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl