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pGL4-phPLAUR (uPAR) (#RDB07312)

Promoter collection, Human PLAUR promoter

Alternative name U2665 (2043)-6
Clone info. PCR amplified human PLAUR promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 43671502 to 43670283(NC_000019.10); relatively -1157 to +63, where +1 corresponds to 1 nt of NM_002659.4.
Comment PCR cloning, forward primer: ttgagacagggtctcgctct; reverse primer: ctcctccgccacaaacttc. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human plasminogen activator, urokinase receptor genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
 
Remarks, protocol and/or map (pdf) RDB07312.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07312 pGL4-phPLAUR (uPAR) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPLAUR (uPAR) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07312).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19225_B1Hbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site, insert 5'
Sequence file: RDB19225_B1Hba.seq check
>07312_19225_B1Hb_1_pGL4ins_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCC NNGTGCAAGT GCAGGTGCCA GAACATTTCT CTGGCCTAAC
   61 TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG GATTTGAGAC AGGGTCTCGC TCTGTTGCCC
  121 AGGTTGGAGT GCAGTGGTGC AATCATAGCT CACTGCAGCT TCCAGTTCCC AGGTTCAAGC
  181 GGTCCTCCCA CCTTAGCCTC CCAACTACCT AGGACTACAA GTACGGGCCA CTGCACCCAG
  241 CTAATTTTAT TTTATTTTTA ATTTCTTTTG GAAAGATGAG GGCTCTTTAT GTTTCCCAAG
  301 CTGATCTTGA ACTCCTGGCC CCAAGTGATC CTCCTGCCTT GGCTTCCCAA AGCATTGGTG
  361 TTACAGGCAT GAGCCACCTC ATCTGACCTC TTCTAAAATA TTATAAAATA AATACAGACA
  421 TCAAAATGAA AACAAAAGAA AAAGAAAAAA CAAAACAAAA ATCCCCAAAA CAACAACAAC
  481 AAAAAATGCA GACATCAATA ACATTCACCA CTAAATACTC TAGTAANNNN NNGCAAAAAC
  541 NAACAAACAA ACAAAAACCA GAATCCCACC TAAGCATACN GCCATTGTCG NAACAGTGAT
601 ATCNACTTTT TTGNNTGANG NGNNNNNNNN NN
//
Primer: pGL4ins_R (Pr0666)
Region: insert 3'
Sequence file: RDB19225_B1Hbb.seq check
>07312_19225_B1Hb_1_pGL4ns_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGCGCTGGGC CCTTCTTAAT GTTTTTGGCA TCTTCCNTGG
   61 TGGCTTTACC AACAGTACCG GATTGCCAAG CTTGGCCGCC GAGGCCAGAT CTTGATCTCC
  121 TCCGCCACAA ACTTCCCTTC CCGGGGCGCG GCCGGCTGGT GGTGAAGGGG CTGGCTCGGC
  181 CCTGACTCAT GGAGTTGTGA TCACAGCTCT GTCCCCAGAT TGCCTGGGTG CAAATCCCAG
  241 TTCTCTCTCT TCCTAACGTG GGACCCGGGG CAATCGCTCT CCACTGCTGT AAAATGAGGA
  301 TAAAAACTTT GACGGTAAAT ATGAATGTGG CTGATTATTT GGCTAACAGG ATTTTTGTTA
  361 GTTTTGTCAG GAGGGATACT GGTATTTTGG AAAATGTCCT TATCTAGAGA TGCATCCTGA
  421 AATATGCATC TCTTAAACAC TTAAAACACT TAACCCTTGC TTTGCTTCCC GCTGTGCCCC
  481 CAGCTCCTGG ACCAGTGCCC AGCACGAAGT AGGTCCTTGG TAAATGTTAA AGGAATGTGT
  541 TGAGCCCTCC ATTTTGGCCA GCAGCTATTC CAGGCATTGC ACCTACATTA AAACACTTAA
  601 TGNTGAAACC CCGTCTCNAC TAAAANNACA AAAANTNNNC CNGGCNTANN GNNGTGTGTG
  661 TNNTACTTAN NNNGCTGANN CNNNNAATNN CTTGNNNCNN GGANGCNNAN GNTGGGTTGN
721 NNNGNNNNNA NNNNNNNCNN NNNNCNNCNN NNNNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 pA,Luc 3'
Sequence file: RDB19225_B1Hbc.seq check
>07312_19225_B1Hb_1_SV40pA_R_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTTTTTNNC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
   61 TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
  121 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
  181 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
  241 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
  301 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
  361 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
  421 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
  481 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
  541 GCGTTTGTAG CCTCGGGGTT GTTAACGTAN CCGCTCATGA TCATGGGGCC NCGGACGCNN
  601 AGCTCGNCNN NNNTGGTNNN NNNCNAGNGT CTTACCGGTG TCCNAGTCCA CCACNNTANC
661 CTCNNNNAAN GGNNNCCNNC TTNNNANTGN NNNAGGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles