RDB07303 - pGL4-phAPOC3

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pGL4-phAPOC3 (#RDB07303)

Promoter collection, Human APOC3 promoter

Alternative name	U2653 (2031)-1
Clone info.	PCR amplified human APOC3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 116829078 to 116830062(NC_000011.10); relatively -829 to +156, where +1 corresponds to 1 nt of NM_000040.3. Consequently, this region contains 1st exon (1 - 33nt of NM_000040.1, 116,829,907 - 116,829,940nt) and a part of intron (116,829,941 - 116,830,062nt).
Comment	PCR cloning, forward primer: ccaggaaggtgaacgagaga; reverse primer: tccgaggcttccttagctct. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human apolipoprotein C-III genomic DNA
Depositor\|Developer	DNA Bank, \| Pan, Jianzhi \|

Remarks, protocol and/or map (pdf)	RDB07303.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07303	pGL4-phAPOC3	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phAPOC3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07303).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07303_A7Eop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07303_A7Eoa.seq
>RDB07303_07303_A7Eo_pGL4-4174F_E01_13_ABI24.ab1 1 ATAGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TCCAGGAAGG TGAACGAGAG AATCAGTCCT GGTGGGGGCT GGGGAGGGCC CCAGACATGA 121 GACCAGCTCC TCCCCCAGGG GATGTTATCA GTGGGTCCAG AGGGCAAAAT AGGGAGCCTG 181 GTGGAGGGAG GGGCAAAGGC CTCGGGCTCT GAGCGGCCTT GGCCCTTCTC CACCAACCCC 241 TGCCCTACAC TAAGGGGGAG GCAGCGGGGG GCACACAGGG TGGGGGCGGG TGGGGGGCTG 301 CTGGGTGAGC AGCACTCGCC TGCCTGGATT GAAACCCAGA GATGGAGGTG CTGGGAGGGG 361 CTGTGAGAGC TCAGCCCTGT AACCAGGCCT TGCCGGAGCC ACTGATGCCT GGTCTTCTGT 421 GCCTTTACTC CAAACACCCC CCAGCCCAAG CCACCCACTT GTTCTCAAGT CTGAAGAAGC 481 CCCTCACCCC TCTACTCCAG GCTGTGTTCA GGGCTTGGGG CTGGTGGAGG GAGGGGCCTG 541 AAATTCCAGT GTGAAAGGCT GAGATGGGCC CGAGGCCCCT GGCCTATGTC CAAGCCATTT 601 CCCCTCTCAC CAGCCTCTCC CTGGGGAGCC AGTCAGCTAG GAAGGAATGA GGGCTCCCCA 661 GGCCCACCCC CAGTTCCTGA GCTCATCTGG GCTGCAGGGC TGGCGGGACA GCAGCGTGGA 721 CTCAGTCTCC TAGGGATTTC CCAACTCTCC CGCCCGCTTG CTGCATCTGG ACACCCTGCC 781 TCAGGCCCTC ATCTCCACTG GTCAGCAGGT GACCTTTGCC CAGCGCCCTG GGTCCTCAGT 841 GCCTGCTGCC CTGGAGATGA TATAAAACAG GTCAGAACCC TCCTGCCTGT CTGCTCAGTT 901 CATCCCTAGA GGCAGCTGCT CCAGGTAATG CCCTCTGGGG AGGGGAAAGA GGAGGGGGGA 961 GGGAAGGAAT GAAGAG //
Primer: pGL4-136R Sequence file: RDB07303_A7Eob.seq
>RDB07303_07303_A7Eo_pGL4-136R_F01_16_ABI24.ab1 1 GCGTCCTATG GGTTTTTTTG GCATCTTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC 61 CAAGCTTGGC CGCCGAGGCC AGATCTTGAT TCCGAGGCTT CCTTAGCTCT AGCAAGTGCT 121 TCTCCAGGCT TGCTGGCTGG GCTGGGCAGG GAGCTCCTCT TGCCCCTCTT CATCCTCCTC 181 CCCTCCTCTT TCCCCTCCCC AGAGGGCATT ACCTGGAGCA GCTGCCTCTA GGGATGAACT 241 GAGCAGACAG GCAGGAGGGT TCTGACCTGT TTTATATCAT CTCCAGGGCA GCAGGCACTG 301 AGGACCCAGG GCGCTGGGCA AAGGTCACCT GCTGACCAGT GGAGATGAGG GCCTGAGGCA 361 GGGTGTCCAG ATGCAGCAAG CGGGCGGGAG AGTTGGGAAA TCCCTAGGAG ACTGAGTCCA 421 CGCTGCTGTC CCGCCAGCCC TGCAGCCCAG ATGAGCTCAG GAACTGGGGG TGGGCCTGGG 481 GAGCCCTCAT TCCTTCCTAG CTGACTGGCT CCCCAGGGAG AGGCTGGTGA GAGGGGAAAT 541 GGCTTGGACA TAGGCCAGGG GCCTCGGGCC CATCTCAGCC TTTCACACTG GAATTTCAGG 601 CCCCTCCCTC CACCAGCCCC AAGCCCTGAA CACAGCCTGG AGTAGAGGGG TGAGGGGCTT 661 CTTCAGACTT GAGAACAAGT GGGTGGCTTG GGCTGGGGGG TGTTTGGAGT AAAGGCACAG 721 AAGACCAGGC ATCAGTGGCT CCGGCAAGGC CTGGTTACAG GGCTGAGCTC TCACAGCCCC 781 TCCCAGCACC TCCATCTCTG GGGTTTCAAT CCAGGCAGGC GAGTGCTGCT CACCCAGCAG 841 CCCCCCACCC GCCCCCACCC TGTGTGCCCC CCGCTGCCTC CCCCTTAGTG TAGGGCAGGG 901 GGTTGGGTGG AGAAGGGCCA AGGCCGCTCA GAGCCCGAGG CCTTTGCCCC TCCCTCCACC 961 AAGGGCTCCC CTATTTTGCC CTCTGGACCC ACTGATAACA TCCCTGGGGG AGGAGCTGGT 1021 CTCATGTCTG GGGGCCCTCC CCAGCCCCAA CCAGGACTGG ATTCTCTCGT TCAACCTTTC 1081 CTGGGATCCT CGAGGGCTAG CGGAGCTCAA GTAACCGGCA AGTTAGGCCA GAGAAAATGT 1141 TTCCTGGTCA CCCTGGTCA //
Primer: pAxCALNL_F1 Sequence file: RDB07303_A7Eoc.seq
>RDB07303_07303_A7Eo_pAxCALNL_F1_G01_19_ABI24.ab1 1 CATTAATGTA TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT 61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT 121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT 181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT 241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC 301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG 361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT 421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA 481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC 541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG 601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA 661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA 721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT 781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA 841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAAGCTGCGC AAGAATAGCT 901 CCTCCTCGAA GCGGTACATG AGCACGACCC GGAAAGCCGC AGATCAAGTA GCCCAGCGTG 961 GTGAACATGC CGAAGCCGTG GTGAAATGGG CACCACGCTG AGGATAGCGG TGTCGGGGAT 1021 GATCTGGTTG CCGAAGGATG GGGGTCGCGG GCATGACTGA TCCGGACACA GCGTGCATGC 1081 GGTAGGCTAC GCCTGGGCAT CGGTTACTGC TCACTACTGA TTCATGAATC AGGGGGCCGA 1141 ATTGGT //