RDB07303 - pGL4-phAPOC3

pGL4-phAPOC3 (#RDB07303)

Promoter collection, Human APOC3 promoter

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Remarks, protocol and/or map (pdf)	RDB07303.pdf
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Alternative name	U2653 (2031)-1
Clone info.	PCR amplified human APOC3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 116829078 to 116830062(NC_000011.10); relatively -829 to +156, where +1 corresponds to 1 nt of NM_000040.3. Consequently, this region contains 1st exon (1 - 33nt of NM_000040.1, 116,829,907 - 116,829,940nt) and a part of intron (116,829,941 - 116,830,062nt).
Comment	PCR cloning, forward primer: ccaggaaggtgaacgagaga; reverse primer: tccgaggcttccttagctct. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Growth conditions	37C, LB+Amp
Gene/insert name	Human apolipoprotein C-III genomic DNA
Depositor\|Developer	DNA Bank, \| Pan, Jianzhi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee
RDB07303	pGL4-phAPOC3	DNA solution

How to cite this biological resource

Materials & Methods section:

The pGL4-phAPOC3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07303).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report	Yamashita, A., Berberine promotes K48-linked polyubiquitination of HNF4α, leading to the inhibition of HBV replication. Antiviral Res. 232: 106027 (2024). PMID 39489302. [PubMed] [Article] [RRC of NBRP]

Resource search

Link

Other resources in our bank

human apolipoprotein C-III

External Database
Human apolipoprotein C-III

Reference sequence