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pGL4-phPTGS2 (hCox-2) (#RDB07300)

Promoter collection, Human PTGS2 promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB07300.pdf
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name U2648 (2026)-8
Clone info. PCR amplified human PTGS2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 186681620 to 186680368(NC_000001.11); relatively -1197 to +56, where +1 corresponds to 1 nt of NM_000963.4.
Comment PCR cloning, forward primer: aactgtcaaaatctcccttcca; reverse primer: ctgctgaggagttcctggac. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee
RDB07300 pGL4-phPTGS2 (hCox-2) DNA solution

How to cite this biological resource

Materials & Methods section:

The pGL4-phPTGS2 (hCox-2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07300).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles