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pGL4-phBRCA1 (#RDB07296)

Promoter collection, Human BRCA1 promoter

Alternative name U2642 (2020)-1
Clone info. PCR amplified human BRCA1 promoter sequence was inserted into a firefly luciferase vector. Genomic DNA (43,125,397 - 43,126,526nt of NC_000017.11) corresponding to 1 to 87nt of NM_007294.3 and 1.0 kb up-stream was cloned.
Comment PCR cloning, forward primer: tcctcccttgaacttctcca; reverse primer: atctgtaattcccgcgcttt. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human breast cancer 1, early onset genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
 
Remarks, protocol and/or map (pdf) RDB07296.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07296 pGL4-phBRCA1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phBRCA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07296).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07296_A7I6p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-136R ()
Region: Luc,insert3'
Sequence file: RDB07296_A7I6b.seq check
>07296_07296_A7I6_pGL4-136R_B01_02_ABI08.ab1
    1 GCCTAAAGTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
   61 GCCGCCGAGG CCAGATCTTG AATCTGTAAT TCCCGCGCTT TTCCGTTGCC ACGGAAACCA
  121 AGGGGCTACC GCTAAGCAGC AGCCTCTCAG AATACGAAAT CAAGGTACAA TCAGAGGATG
  181 GGAGGGACAG AAAGAGCCAA GCGTCTCTCG GGGCTCTGGA TTGGCCACCC AGTCTGCCCC
  241 CGGATGACGT AAAAGGAAAG AGACGGAAGA GGAAGAATTC TACCTGAGTT TGCCATAAAG
  301 TGCCTGCCCT CTAGCCTCTA CTCTTCCAGT TGCGGCTTAT TGCATCACAG TAATTGCTGT
  361 ACGAAGGTCA GAATCGCTAC CTATTGTCCA AAGCAGTCGT AAGAAGAGGT CCCAATCCCC
  421 CACTCTTTCC GCCCTAATGG AGGTCTCCAG TTTCGGTAAA TATAAGTAAT AAGGATTGTT
  481 GGGGGGGTGG AGGGAAATAA TTATTTCCAG CATGCGTTGC GGAATGAAAG GTCTTCGCCA
  541 CAGTGTTCCT TAGAAACTGT AGTCTTATGG AGAGGAACAT CCAATACCAG AGCGGGCACA
  601 ATTCTCACGG AAATCCAGTG GATAGATTGG AGACCTGTGC GCGCTTGTAC TTGTCAACAG
  661 TTATGGACTG GAGTGTTATG TTTTCGTATT TTGAAAGCAG AAACTAGGCC TTAAAAAGAT
  721 ACGTACAACT CTTTAGGGAG ACTACAATTC CCATCCAGCC CCAGGAGTCT GGGGCAAGTA
  781 GTCTTGTAAG GTCAGTGGCC TGCGGGGACG CAGTGAGCGC CGAATTTGCC TGGGGCAGGG
  841 GAAATGCGCT CTGGCCCATG TCTGCGCACT CGTAGTTCCA CCCCCTCAGC CCCAGTGTTT
  901 GTTATTTTTC GGGTTCAGCT TGCTTTTTGC CCCGTCTCCG TCGACGCAAT CGCCACCAGT
  961 CAATGGGGGT GGGTCGTTTT TGAGGGACAA GTGGTAAGAG CCAAATCTTT CTTGGCGAAA
 1021 AACGCGGAGG AAACGGGACT AGTTTACTGT CTTTGTCCCG CCCATGTTAG ATTCCACCCC
1081 CCCACAGAAG ATAGCCGCCA GAGCTGGCAG GCGGAACGGA TTCCTTTTTG CCAT
//
Primer: pAxCALNLF1 ()
Region: Sv40a(A),Luc
Sequence file: RDB07296_A7I6c.seq check
>07296_07296_A7I6_pAxCALNL_F1_C01_03_ABI08.ab1
    1 ATAGGGATAT CTTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
   61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
  121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
  181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
  241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
  301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
  361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
  421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
  481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
  541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
  601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
  661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
  721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
  781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
  841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAAGCTGCGC AAGAATAGCT
  901 CCTCCTCGAA GCGGTACATG AGCACGACCC GAAAGCCGCA GATCAAGTAG CCCAGCGTGG
  961 TGAAACATGC CGAAGCCGTG GTGAAATGGC ACCCACGGCT GAGGATAGCC GGTGTCGGGG
 1021 GATGATCTGG GTTTGCCCGA AGATGGGGGT CGCGGGCATG ACTGAATTCG GACACAGCGT
1081 TGCGGATGCG GTTTAGGACT ACGCCCCTTT GGGGCCA
//
Primer:
Sequence file: RDB07296_A7I6d.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles