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pTky-1

Complete genome of JC virus, PML-type

Catalog number RDB07252
Resource name pTky-1
Clone info. Complete genome of JC virus, PML-type. JCV genomic DNA derived from the brain of a Japanese PML patient.
Known differences: G to T substitution (Trp to Leu) at 475 nt of AB038254.1.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genomic DNA
Reference of nucleotide sequence AB038254 (DDBJ accession number)
Depositor Yogo, Yoshiaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Jpn. J. Med. Sci. Biol., 47, 167-178 (1994)).
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RDB07252 pTky-1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Kato, K., Phylogenetic comparison between archetypal and disease-associated JC virus isolates in Japan. Jpn. J. Med. Sci. Biol., 47, 167-178 (1994). PMID 7823412.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07252_A9F3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F RDB07252_A9F3a.seq
>07252_07252_A9F3_2_pUC-CAPbs_up_F_E04_13_ABI24.ab1
    1 TACGGGGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA
   61 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT
  121 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC
  181 TAGAGGATCC CAACACTCTA CCCCACCTAA GAAGAAAAAA AAGGTAGAAG ACCCTAAAGA
  241 CTTTCCTGTA GATCTGCATG CATTCCTCAG TCAAGCTGTG TTTAGTAATA GAACTGTTGC
  301 TTCTTTTGCT GTGTATACCA CTAAAGAAAA AGCTCAAATT TTATATAAGA AACTTATGGA
  361 AAAATATTCT GTAACCTTTA TAAGTAGACA TGGTTTTGGG GGTCATAATA TCTTGTTTTT
  421 TTTAACACCA CATAGACATA GAGTTTCAGC AATTAATAAT TACTGTCAAA AACTATGTAC
  481 CTTTAGTTTT TTAATTTGTA AAGGTGTGAA TAAGGAATAC TTGTTTTACA GTGCCTTGTG
  541 TAGACAGCCA TATGCAGTAG TGGAAGAAAG TATTCAGGGG GGCCTTAAGG AGCATGACTT
  601 TAACCCAGAA GAACCAGAAG AAACTAAGCA GGTTTCATGG AAATTAGTTA CACAGTATGC
  661 TTTGGACACC AAGTGTGAGG ATGTTTTCTT GCTTATGGGC ATGTACTTAG ACTTTCAGGA
  721 AAACCCTCTG CAATGCAAAA AATGTGAAAA AAAGGATCAG CCAAATCACT TTAACCATCA
  781 TGAAAAGCAC TATTATAATG CCCAAATTTT TGCAGACAGC AAAAATCAAA AAAGCATTTG
  841 CCAGCAGGCT GTTGATACTG TAGCAGCCAA ACAAAGGGTT GACAGTATCC ATATGACCAG
  901 AGAAGAAATG TTAGTTGAAA AGGTTTAATT TTTTGCTTGA TAAAATGGAC TTAATTTTTG
  961 GGGCACATGG CAATGCTGTT TTAGAGCAAT ATATGGCTGG GGTGGCCCTG GATTCATTGT
 1021 TTGCTGCCTC AAATGGACAC TGTCATTTAT GAGTTCTTAA AATGCATTGT ATAAACATTT
 1081 CCAAAAAAAA AGGTACCTGC TATTAGGATG ATTAGACAGT GGCAAAACTT AACTTTTAGC
1141 TTGCCAAGC
//
Primer: pUC-M13_-100 RDB07252_A9F3b.seq
>07252_07252_A9F3_2_pUC-M13_-100_F04_16_ABI24.ab1
    1 CGCCAGGCGA TTAGTTGGGT ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG
   61 CCAGTGAATT CGAGCTCGGT ACCCGGGGAT CCTGTGTTTT CATCATCACT GGCAAACATT
  121 TCTTCATGGC AAAACAGGTC TTCATCCCAC TTTTCATTAA ATGTATTCCA CCAGGATTCC
  181 CATTCATCTG TTCCATAGGT TGGCACCTAA AAACAAAAAA TTAAGTTTAT TGTAAAAAAC
  241 AAAATGCCCT GCAAAAGAAA AATTGTGGTT TACCTTAAAG CTTTAGATCC CTGTAGGGGG
  301 TGTCTCCAAG AACCTTCTCC CAGCAATGAA GAGCTTCTTG GGTTAAGTCA CACCCAAACC
  361 ATTGTCTGAA GCAATCAAAG CAATAGCAAT CTATCCACAC AAGTGGGCTG CTTCTTAAAA
  421 ATTTTCTGTT TCTATGCCTT AATTTTAGCA TGCACATTAA ACAGGGGCAA TGCACTGAAG
  481 GATTAGTGGC ACAATTAGGC CATTCCTTGC AATAAAGGGT ATCAGAATTA GGAGGAAAAT
  541 CACAACCAAC CTCTGAACTA TTCCATGTAC CAAAATCAGG CTGATGAGCA ACTTTTACAC
  601 CTTGTTCCAT TTTTTTATAT AAAAAATTCA TTCTCTTCAT TTTGTCTTCG TCCCCACCTT
  661 TATCAGGGTG AAGTTCTTTG CATTTTTTCA GATAAGCTTT TCTCATGACA GGAATGTTTC
  721 CCCATGCAGA TCTATCAAGG CCTAATAAAT CCATGAGCTC CATGGATTCC TCCCTATTCA
  781 GCACTTTGTC CATTTTTGCT TTTTGTAGCA AAAAATTAGT GCAAAAAAGG GAAAAACAAG
  841 GGAATTTCCC TGGCCTCCTA AAAAGCCTCC ACGCCCTTAC TACTTCTGAG TAAGCTTGGA
  901 GGCGGAGGCG GCCTCGGCCT CCTGTATATA TAAAAAAAAA GGAAAGGTAG GGAGGAGCTG
  961 GCTAAAACTG GGATGGCTGC CAGCCCAAGC ATGAGCTCAT ACACCACTCC CATAGTCCCT
 1021 TTAATCACAA GTAACAAAGC ACAAGTAAAG GGAGAGCTGG GCTAAAACTG GATGGCTGCC
 1081 AGCCTAGCAT GAGCTCATAT AATCACAGTT AACCAAGGCC ACAGGGGAAT GGTAAGGAAG
1141 CCTTGG
//
Primer: JCsp_f1 RDB07252_A9F3c.seq
>07252_07252_A9F3_2_JCsp_f1_C01_07_ABI24.ab1
    1 TAACATATGA AGATGGCCCC AACAAAAAGA AAAGGAGAAA GGAAGGACCC CGTGCAAGTT
   61 CCAAAACTTC TTATAAGAGG AGGAGTAGAA GTTCTAGAAG TTAAAACTGG GGTTGACTCA
  121 ATTACAGAGG TAGAATGCTT TTTAACTCCA GAAATGGGTG ACCCAGATGA GCATCTTAGG
  181 GGTTTTAGTA AGTCAATTTC TATATCAGAT ACATTTGAAA GTGACTCCCC AAATAAGGAC
  241 ATGCTTCCTT GTTACAGTGT GGCCAGAATT CCACTACCCA ATCTAAATGA GGATCTAACC
  301 TGTGGAAATA TACTAATGTG GGAGGCTGTG ACCTTAAAAA CTGAGGTTCT AGGGGTGACA
  361 ACTTTGATGA ATGTGCACTC TAATGGTCAA GCAACTCATG ACAATGGTGC AGGAAAGCCA
  421 GTGCAGGGCA CCAGCTTTCA TTTTTTTTCT GTTGGGGGGG AGGCTTTAGA ATTACAGGGG
  481 GTGGTTTTTA ATTACAGAAC AAAGTACCCA GATGGAACAA TTTTTCCAAA GAATGCAACA
  541 GTGCAATCTC AAGTAATGAA CACAGAGCAC AAGGCGTACC TAGATAAGAA CAAAGCATAT
  601 CCTGTTGAAT GTTGGGTTCC TGATCCCACC AGAAATGAAA ACACAAGATA TTTTGGGACA
  661 CTAACAGGAG GAGAAAATGT TCCTCCAGTT CTTCATATAA CAAACACTGC CACAACAGTG
  721 CTGCTTGATG AATTTGGTGT TGGGCCACTT TGCAAAGGTG ACAACTTGTA TTTGTCAGCT
  781 GTTGATGTTT GTGGAATGTT TACTAACAGA TCTGGTTTCC AGCAGTGGAG AGGACTGTCC
  841 AGATATTTTA AGGTTCAGCT CAGAAAAAGG AGGGTTAAAA ACCCCTACCC AATTTCTTTC
  901 CTTCTTACTG ATTTGATTAA CAGAAAGACC CCTAGAGTTG ATGGGCAGCC TATGTATGGT
  961 ATGGATGCTC AGGTAGAGGA GGTTAGAGTT TTTTGAGGGG ACAGAGGAAC TTTCCAGGGG
 1021 ACCCAGACAT GATGAGATAT GTTTGACAGA TATGGACAGG TTGCAAACAA GGATGGCTGT
 1081 ATCAAAAAGC CTTTAATTGG TATATGCCAG TTACATTTTA ATTAAAGGTA TTACCCAGCC
1141 CTTTTACCTT TTA
//
Primer: JCsp_R2 RDB07252_A9F3d.seq
>07252_07252_A9F3_2_JCsp_R2_C03_09_ABI24.ab1
    1 TGTTTAAGTA AAAGCTGGGT TATACTTTAT TAAAATGTAC TGCATATTAC AATAAAGGCT
   61 TTTGATTACA GCATCTTTGT TTGCAACTGT CCATATCTGT CAACATATCT CATCATGTCT
  121 GGGTCCCCTG GAAGTTCCTC TGTCCCCTCA AAAACTCTAA CCTCCTCTAC CTGAGCATCC
  181 ATACCATACA TAGGCTGCCC ATCAACTCTA GGGGTCCTTC TGTTAATCAA ATCAGTAAGA
  241 AGGAAAGAAA TTGGGTAGGG GTTTTTAACC CTCCTTTTTC TGAGCTGAAC CTTAAAATAT
  301 CTGGACAGTC CTCTCCACTG CTGGAAACCA GATCTGTTAG TAAACATTCC ACAAACATCA
  361 ACAGCTGACA AATACAAGTT GTCACCTTTG CAAAGTGGCC CAACACCAAA TTCATCAAGC
  421 AGCACTGTTG TGGCAGTGTT TGTTATATGA AGAACTGGAG GAACATTTTC TCCTCCTGTT
  481 AGTGTCCCAA AATATCTTGT GTTTTCATTT CTGGTGGGAT CAGGAACCCA ACATTCAACA
  541 GGATATGCTT TGTTCTTATC TAGGTACGCC TTGTGCTCTG TGTTCATTAC TTGAGATTGC
  601 ACTGTTGCAT TCTTTGGAAA AATTGTTCCA TCTGGGTACT TTGTTCTGTA ATTAAAAACC
  661 ACCCCCTGTA ATTCTAAAGC CTCCCCCCCA ACAGAAAAAA AATGAAAGCT GGTGTCCTGC
  721 ACTGGCTTTC CTGCACCATT GTCATGAGTT GCTTGACCAT TAGAGTGCAC ATTCATCAAA
  781 AGTTGTCACC CCTAGAACCT CAGTTTTTAA GGGTCACAGC CTCCCACATT AGTATATTTT
  841 CCACAGGTTA GATCCTCATT TAGATTGGGT AGTGGGAATT CTGGCCACAC TGTAACAAGG
  901 GAAAGCATGT CCTTATTTGG GGGAGTCACT TTCAAATGTA TCTGATATAG AAATTGACTT
  961 ACTAAAAACC CCCTAAGATG CTCATCTGGG TCACCCCATT TTCTGGGAGT TAAAAAAGGC
 1021 ATTTCTACCT CTGTAAATTG GAGGTCAAAC CCCAGGTTTT TAACTTTCTA GAAACTTTCT
 1081 TACTCCCTCC TCTTATAAGA AGGTTTTTGA AACCTTTGCC ACGGGGTTCC CTTCCGTTTT
1141 CCTTCCATAC A
//
Primer: JCsp_f4 RDB07252_A9F3e.seq
>07252_07252_A9F3_2_JCsp_f4_G01_19_ABI24.ab1
    1 AACCCTCAGG GGATTCCTCC CTATTCAGCA CTTTGTCCAT TTTTGCTTTT TGTAGCAAAA
   61 AATTAGTGCA AAAAAGGGAA AAACAAGGGA ATTTCCCTGG CCTCCTAAAA AGCCTCCACG
  121 CCCTTACTAC TTCTGAGTAA GCTTGGAGGC GGAGGCGGCC TCGGCCTCCT GTATATATAA
  181 AAAAAAGGGA AGGTAGGGAG GAGCTGGCTA AAACTGGATG GCTGCCAGCC AAGCATGAGC
  241 TCATACACCA CTCCCATAGT CCTTAATCAC AAGTAAACAA AGCACAAGGG GAAGGGAGGA
  301 GCTGGCTAAA ACTGGATGGC TGCCAGCCAA GCATGAGCTC ATATTAATCA CAAGTAAACA
  361 AAGCACAAGG GGAAGGGAGG AGCTGGCTAA AACTGGATGG CTGCCAGCCA AGCATGAGCT
  421 CATACACCAC TCCCATAGTC CTTAATCACA AGTAAACAAA GCACAAGGGG AAGTGGAAAG
  481 CAGCCAAGGG AACATGTTTT GCGAGCCAGA GCTGTTTTGG CTTGTCACCA GCTGGCCATG
  541 GTTCTTCGCC AGCTGTCACG TAAGGCTTCT GTGAAAGTTA GTAAAACCTG GAGTGGAACT
  601 AAAAAAAGAG CTCAAAGGAT TTTAATTTTT TTGTTAGAAT TTTTGCTGGA TTTTTGCACA
  661 GGTGAAGACA GTGTAGACGG GAAAAAAAGG CAGAAACACA GTGGTTTGAC TCAGCAGACA
  721 TACAGTGCTT TGCCTGAACC AAAAGCTACA TAGGTAAGTA ATGTTTTTTT TTGTGTTTTC
  781 AGGTTCATGG GTGCCGCACT TGCACTTTTG GGGGACCTAG TTGCTACTGT TTCTGAGGCT
  841 GCTGCTGCCA CAGGATTTTC AGTAGCTGAA ATTGCTGCTG GAGAGGCTGC TGCTACTATA
  901 GAAGTTGAAA TTGCATCCCT TGCTACTGTA GAGGGGATTA CAAGTACCTC TGAGGCTATA
  961 GCTGCAATAG GCCTTACTCC CTGAAACATA TGCTGTAATT ACTGGAGCTC CGGGGGCTGT
 1021 AGCTGGGGTT TGCTGCATTG GTTCAACTGT AACTGGTGGG TAGTGCTATT GCTTCAGTTG
 1081 GATATAGATT TTTTGGCTGA CTGGGATCAT TAAAGTTCAC AGATTGGCCT TTTTTCAGGC
1141 CAGAGCCCAG G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kato, K., Phylogenetic comparison between archetypal and disease-associated JC virus isolates in Japan. Jpn. J. Med. Sci. Biol., 47, 167-178 (1994). PMID 7823412.

2019.07.09

GNP_filter3_RDBDEP_html_190431.pl