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pNY-1B

Complete genome of JC virus, PML-type

Catalog number RDB07251
Resource name pNY-1B
Clone info. Complete genome of JC virus, PML-type. JCV genomic DNA derived from the brain of an American PML patient.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genomic DNA
Reference of insert sequence D12575, D11366 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |
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External Database
Hum_Polyoma JCV

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Virus Genes., 8, 99-105 (1994)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Virus Genes., 8, 99-105 (1994))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07251 pNY-1B DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07251_A3Kjp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: reverse-2
            Region: insert (M13 rev side)
            Sequence file: RDB07251_A3Kja.seq check
            >07251_07251_B0KrF_Reverse2_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNTNGN ATGCCTGCAG GTCGACTCTA GAGGANCCTG TGTTTTCATC
               61 ATCACTGGCA AACATTTCTT CATGGCAAAA CAGGTCTTCA TCCCACTTCT CATTAAATGT
              121 ATTCCACCAG GATTCCCATT CATCTGTTCC ATAGGTTGGC ACCTAAAAAA AAACAATTAA
              181 GTTTATTGTA AAAAACAAAA TGCCCTGCAA AAGAAAAATA GTGGTTTACC TTAAAGCTTT
              241 AGATCCCTGT AGGGGGTGTC TCCAAGAACT TTCTCCCAGC AATGAAGAGC TTCTTGGGTT
              301 AAGTCACACC CAAACCATTG TCTGAAGCAA TCAAAGCAAT AGCAATCTAT CCACACAAGT
              361 GGGCTGCTTC TTAAAAATTT TCTGTTTCTA TGCCTTAATT TTAGCATGCA CATTAAACAG
              421 GGGCAATGCA CTGAAGGATT AGTGGCACAG TTAGGCCATT CCTTGCAATA AAGGGTATCA
              481 GAATTAGGAG GAAAATCACA ACCAACCTCT GAACTATTCC ATGTACCAAA ATCAGGCTGA
              541 TGAGCAACTT TTACACCTTG TTCCATTTTT TTATATAAAA AATTCATTCT CTTCATCTTG
              601 TCTTCGTCCC CACCTTTATC AGGGTGGANT TCTTTGCATT TTTTCANATA AGCTTTTCTC
            661 ATGACAGGNA TGNTCCCNCN NTGCAGANCT ATCNNNGNNN NNNNNNN
            //
            Primer: JCV-H_F
            Region: insert (D12575.1)
            Sequence file: RDB07251_A3Kjb.seq check
            >07251_07251_B0KrF_JCV-H_F_G02_20_ABI24.ab1
                1 NNNNNNNTNN NNNNNNANNN NNNTNNTTCT CTTCNTCTTG TCTTCGTCCC CACCTTTATC
               61 AGGGTGGAGT TCTTTGCATT TTTTCAGATA AGCTTTTCTC ATGACAGGAA TGTTCCCCCA
              121 TGCAGACCTA TCAAGGCCTA ATAAATCCAT AAGCTCCATG GATTCCTCCC TATTCAGCAC
              181 TTTGTCCATT TTAGCTTTTT GCAGCAAAAA ATTACTGCAA AAAAGGGAAA AACAAGGGAA
              241 TTTCCCTGGC CTCCTAAAAA GCCTCCACGC CCTTACTACT TCTGAGTAAG CTTGGAGGCG
              301 GAGGCGGCCT CGGCCTCCTG TATATATAAA AAAAAGGGAA GGTAGGGAGG AGCTGGCTAA
              361 AACTGGATGG CTGCCAGCCA AGCATGAGCT CATACCTAGG GAGCCAACCA GCTGACAATA
              421 GTCCTTAATC ACAAGGAGCT GGCTAAAACT GGATGGCTGC CAGCCAAGCA TGAGCTCATA
              481 CCTAGGGAGC CAACCAGCTG ACAATAGTCC TTAATCACAA GTAAACAAAG CACAAGGGGA
              541 AGTGGAAAGC AGCCAAGGGA ACATGTTTTG CGAGCCAGAG CTGTTTTGGC TTGTCACCAG
              601 CTGGCCATGG TTCTTCGCCN GCTGTCACGT AAGGCTTCTG TGAAAGTTAG TAAAACCTGN
              661 ANTGNAACTA AAAAAANANC TCNNNGANTT TNNTTTTTTN GNNANANNTT TTGNNNGNNN
            721 TTTNNNNNNN NNN
            //
            Primer: JCV-M_R1
            Region: insert (D11366.1 5')
            Sequence file: RDB07251_A3Kjc.seq check
            >07251_07251_B0KrF_JCV-M_R1_G01_07_ABI08.ab1
                1 NNNNNNNNCN NNNNNTNGNG NNNNNNNCTT TNNNATGTAT CTGANATAGA TATTGACTTA
               61 CTAAAACCCC TAAGATGCTC ATCTGGGTCA CCCATTTCTG GAGTTAAAAA GCATTCTACC
              121 TCTGTAATTG AGTCAACCCC AGTTTTAACT TCTAGAACTT CTACTCCTCC TCTTATAAGA
              181 AGTTTTGGAA CTTGCACGGG GTCCTTCCTT TCTCCTTTTC TTTTTGTTGG GGCCATCTTC
              241 ATATGCTTCA AGAGCAGGTG TTACAGTCCC GTACAACCCT AAAAGTAAAG GCAACATCCA
              301 TTGAGGAGCA GATCTTTGAT TTGCACCTCC TGGTGCAATA CTTCTTTCTA TAAATTCACC
              361 AGATTGCACA TTTGGGGTTT TTAGATCTAG CCTTTGGGTA ACTTCTTGAA TGCTGTCTGC
              421 ATCATCTATA CTTTGGGTGT ATGAGTGGCC AAAAGAAATA TAGGTTCCCT CCCTTTGGGC
              481 AACTTGCCTT ACCATAGAGG GCCTAACTGG AGACAATCTA GAATAATAGT CTGAAATATA
              541 ATTATATAAG TTAGCTGGTG AATTAACTAT TGCCCAAGTA GTTTCTTCCA AAAACCTTGC
              601 TAAATTTTCA ACAAATAGCT TTTGGGTTCT TCTCTGAATT TCCTGAGANG NTNANNNTGG
              661 CAAATCATCT CTAANNAGAT TCCNAAAANN CNNNNNANTG NGNANAANNA NGGNNNGNNC
            721 CNNNNGNNNN NNNNNN
            //
            Primer: M13_-40
            Region: insert (M13 fwd side)
            Sequence file: RDB07251_A3Kjd.seq check
            >07251_07251_B0KrF_M13_-40_A02_01_ABI08.ab1
                1 NNNNNNNNNN NNCNCNGNNN CCGGGGNNCC CNACACTCTA CCCCACCTAA GAAGAAAAAA
               61 AAGGTAGAAG ACCCTAAAGA CTTTCCTGTA GATCTGCATG CATTCCTCAG TCAAGCTGTG
              121 TTTAGTAATA GAACTGTTGC TTCTTTTGCT GTGTATACCA CTAAAGAAAA AGCTCAAATT
              181 TTATATAAGA AACTTATGGA AAAATATTCT GTAACTTTTA TAAGTAGACA TGGTTTTGGG
              241 GGTCATAATA TTTTGTTTTT CTTAACACCA CATAGACATA GAGTGTCAGC AATTAATAAC
              301 TACTGTCAAA AACTATGTAC CTTTAGTTTT TTAATTTGTA AAGGTGTGAA TAAGGAATAC
              361 TTGTTTTATA GTGCCCTGTG TAGACAGCCA TATGCAGTAG TGGAAGAAAG TATTCAGGGG
              421 GGCCTTAAGG AGCATGACTT TAACCCAGAA GAACCAGAAG AAACTAAGCA GGTTTCATGG
              481 AAATTAGTTA CACAGTATGC CTTGGAAACC AAGTGTGAGG ATGTTTTTTT GCTTATGGGC
              541 ATGTACTTAG ACTTTCAGGA AAACCCACAG CAATGCAAAA AATGTGAAAA AAAGGATCAG
              601 CCAAATCACT TTAACNATCA TGAAAAACAC TATTATAATG CCCAAATTTT TGCAGANAGC
              661 AAAAATCNAA AAAGCANTTG NNNGCNNNCT GTTGANNCTG NANCAGCCAA ACAAANGGTT
            721 GANNNNATCC NCANNNNNNN NNN
            //
            Primer: JCV-M_F3
            Region: insert (D11366.1 5')
            Sequence file: RDB07251_A3Kje.seq check
            >07251_07251_B0KrF_JCV-M_F3_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNAN TTCTAGAAGT TAAAACTGGG GTTGACTCAA TTACAGAGGT
               61 AGAATGCTTT TTAACTCCAG AAATGGGTGA CCCAGATGAG CATCTTAGGG GTTTTAGTAA
              121 GTCAATATCT ATATCAGATA CATTTGAAAG TGACTCCCCA AGTAAGGACA TGCTTCCTTG
              181 TTACAGTGTG GCCAGAATTC CACTACCCAA TCTAAATGAG GATCTAACCT GTGGAAATAT
              241 ACTCATGTGG GAGGCTGTGA CCTTAAAAAC TGAGGTTATA GGGGTGACAA GTTTGATGAA
              301 TGTGCACTCT AATGGTCAAG CAGCTCATGA CAATGGTGCA GGGAAGCCAG TGCAGGGCAC
              361 CAGCTTTCAT TTTTTTTCTG TTGGGGGGGA GGCTTTAGAA TTACAGGGGG TGGTTTTTAA
              421 TTACAGAACA AAGTACCCAG ATGGAACAAT TTTTCCNAAG AANGCCACAG TGCAATCTCA
              481 AGTCATGAAC ACAGAGCACA AGGCGTACCT AGATAAGAAC AAAGCATATC CTGNTGAATG
              541 NTGGNTNNNN GATNCCNCCN GANATGAANA CNNNNGANAT TTNGGGNNNC TNNNNNNNNN
            601 ANNAAANGNN NNNNNNNN
            //
            Primer: JCV-M_R3
            Region: insert (D11366.1 mid)
            Sequence file: RDB07251_A3Kjf.seq check
            >07251_07251_B0KrF_JCV-M_R3_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTNN NNNNNNGNNT CCCCAAGTAA GGACATGCTT CCTTGTTACA
               61 GTGTGGCCAG AATTCCACTA CCCAATCTAA ATGAGGATCT AACCTGTGGA AATATACTCA
              121 TGTGGGAGGC TGTGACCTTA AAAACTGAGG TTATAGGGGT GACAAGTTTG ATGAATGTGC
              181 ACTCTAATGG TCAAGCAGCT CATGACAATG GTGCAGGGAA GCCAGTGCAG GGCACCAGCT
              241 TTCATTTTTT TTCTGTTGGG GGGGAGGCTT TAGAATTACA GGGGGTGGTT TTTAATTACA
              301 GAACAAAGTA CCCAGATGGA ACAATTTTTC CAAAGAATGC CACAGTGCAA TCTCAAGTCA
              361 TGAACACAGA GCACAAGGCG TACCTAGATA AGAACAAAGC ATATCCTGTT GAATGTTGGG
              421 TTCCTGATCC CACCAGAAAT GAAAACACAA GATATTTTGG GACACTAACA GGAGGAGAAA
              481 ATGTTCCTCC AGTTCTTCAT ATAACAAACA CTGCCACAAC AGTGTTGCTT GATGAATTTG
              541 GTGTTGGGCC ACTTTGCAAA GGTGACAACT TATACTTGTC AGCTGTTGAT GTCTGTGGCA
              601 TGTTTACTAA CAGGTCTGGT TCCCAGCAGT GNANAGGACT GTCCAGATAT TTTAANGTTC
              661 AGCTNANNNA AANGANGGTT AAAAACCCNN ACCNNATTTC TTTNNNNCNN ACTNNNTNNN
            721 NTNNNNNNNN NN
            //
            Primer: JCV-B_R2
            Region: insert (D11366.1 3')
            Sequence file: RDB07251_A3Kjg.seq check
            >07251_07251_B0KrF_JCV-B_R2_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNAANNTGTA NNNNNTATTA CAATAAAGGC TTTTGATTAC AGCATTTTTG
               61 TCTGCAACTG TCCATATTTG TCAACATATC TCATCATGTC TGGGTCCCCT GGAAGCTCCT
              121 CTGTTCCCTC AAAAACTCTA ACCTCCTCTA CTTGAGCATC CATGCCATAC ATAGGCTGCC
              181 CATCAACTCT AGGAGTCCTT CTGTTAATTA AATCAGTAAG AAGGAAAGAA ATTGGGTAGG
              241 GGTTTTTAAC CCTCCTTTTC CTTAGCTGAA CCTTAAAATA TCTGGACAGT CCTCTCCACT
              301 GCTGGGAACC AGACCTGTTA GTAAACATGC CACAGACATC AACAGCTGAC AAGTATAAGT
              361 TGTCACCTTT GCAAAGTGGC CCAACACCAA ATTCATCAAG CAACACTGTT GTGGCAGTGT
              421 TTGTTATATG AAGAACTGGA GGAACATTTT CTCCTCCTGT TAGTGTCCCA AAATATCTTG
              481 TGTTTTCATT TCTGGTGGGA TCAGGAACCC AACATTCAAC AGGATATGCT TTGTTCTTAT
              541 CTAGGTACGC CTTGTGCTCT GTGTTCATGA CTTGAGNNTG CACTGTGGCA TTCTTTGGAA
              601 AAATTGTTCC ATCTGGGTAC TTTGTTCTGT AATTAAAAAC CACCCCCTGT AATTCTAAAG
            661 CCTCCCCCCC CNANAGAAAA AAAANGNANN CNNGNGNCNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl