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pNY-1B

Complete genome of JC virus, PML-type

Catalog number RDB07251
Resource name pNY-1B
Clone info. Complete genome of JC virus, PML-type. JCV genomic DNA derived from the brain of an American PML patient.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genomic DNA
Reference of nucleotide sequence D12575, D11366 (DDBJ accession number)
Depositor Yogo, Yoshiaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Virus Genes., 8, 99-105 (1994)).
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RDB07251 pNY-1B DNA solution

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References and tips

Electronic file

Featured content

References

original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07251_A3Kj.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB07251_A3Kja.seq
>07251_07251_A3Kj_2_M13(-40)_E01_13.ab1
    1 GCGCCGTTGA TTCGGAGCTC GGTACCGGGG ATCCCAACAC TCTACCCCAC CTAAGAAGAA
   61 AAAAAAGGTA GAAGACCCTA AAGACTTTCC TGTAGATCTG CATGCATTCC TCAGTCAAGC
  121 TGTGTTTAGT AATAGAACTG TTGCTTCTTT TGCTGTGTAT ACCACTAAAG AAAAAGCTCA
  181 AATTTTATAT AAGAAACTTA TGGAAAAATA TTCTGTAACT TTTATAAGTA GACATGGTTT
  241 TGGGGGTCAT AATATTTTGT TTTTCTTAAC ACCACATAGA CATAGAGTGT CAGCAATTAA
  301 TAACTACTGT CAAAAACTAT GTACCTTTAG TTTTTTAATT TGTAAAGGTG TGAATAAGGA
  361 ATACTTGTTT TATAGTGCCC TGTGTAGACA GCCATATGCA GTAGTGGAAG AAAGTATTCA
  421 GGGGGGCCTT AAGGAGCATG ACTTTAACCC AGAAGAACCA GAAGAAACTA AGCAGGTTTC
  481 ATGGAAATTA GTTACACAGT ATGCCTTGGA AACCAAGTGT GAGGATGTTT TTTTGCTTAT
  541 GGGCATGTAC TTAGACTTTC AGGAAAACCC ACAGCAATGC AAAAAATGTG AAAAAAAGGA
  601 TCAGCCAAAT CACTTTAACC ATCATGAAAA ACACTATTAT AATGCCCAAA TTTTTGCAGA
  661 TAGCAAAAAT CAAAAAAGCA TTTGCCAGCA GGCTGTTGAT ACTGTAGCAG CCAAACAAAG
  721 GGTTGACAGC ATCCACATGA CCAGAGAAGA AATGTTAGTT GAAAGGTTTA ATTTCTTGCT
  781 TGATAAAATG GACTTGATTT TTGGGGCACA TGGCAATGCT GTTTTAGAGC AATATATGGC
  841 TGGGGTGGCC TGGATTCATT GCTTGCTGCC TCAAATGGAC ACTGTTATTT ATGACTTTCT
  901 GAAATGCATT GTATTAAACA TTCCAAAAAA AAGGTACTGG CTATTCAAGG GGCCAATAGA
  961 CAGTGGCAAA ACTACTTTAG CTGCAGCTTT ACTTGATCTC TGTGGGGGAA AGTCATTAAA
 1021 TGTTTAATAT GCCATTAGAA AGATTAAACT TTGAGTTTAG GAGTGGTATA GATCAGTTTA
 1081 TGGGTTTGTA TTTTGAGGAT GTAAACGGCA CTTGGCTGCC AGAAGTTCCA AGGGCAAATT
1141 TTAACCCTTC CAGAG
//
Primer: RDB07251_A3Kjb.seq
>07251_07251_A3Kj_2_Reverse2_E04_13.ab1
    1 AAGACAAGGA ATACGGCCCA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG ATCCTGTGTT
   61 TTCATCATCA CTGGCAAACA TTTCTTCATG GCAAAACAGG TCTTCATCCC ACTTCTCATT
  121 AAATGTATTC CACCAGGATT CCCATTCATC TGTTCCATAG GTTGGCACCT AAAAAAAAAC
  181 AATTAAGTTT ATTGTAAAAA ACAAAATGCC CTGCAAAAGA AAAATAGTGG TTTACCTTAA
  241 AGCTTTAGAT CCCTGTAGGG GGTGTCTCCA AGAACTTTCT CCCAGCAATG AAGAGCTTCT
  301 TGGGTTAAGT CACACCCAAA CCATTGTCTG AAGCAATCAA AGCAATAGCA ATCTATCCAC
  361 ACAAGTGGGC TGCTTCTTAA AAATTTTCTG TTTCTATGCC TTAATTTTAG CATGCACATT
  421 AAACAGGGGC AATGCACTGA AGGATTAGTG GCACAGTTAG GCCATTCCTT GCAATAAAGG
  481 GTATCAGAAT TAGGAGGAAA ATCACAACCA ACCTCTGAAC TATTCCATGT ACCAAAATCA
  541 GGCTGATGAG CAACTTTTAC ACCTTGTTCC ATTTTTTTAT ATAAAAAATT CATTCTCTTC
  601 ATCTTGTCTT CGTCCCCACC TTTATCAGGG TGGAGTTCTT TGCATTTTTT CAGATAAGCT
  661 TTTCTCATGA CAGGAATGTT CCCCCATGCA GACCTATCAA GGCCTAATAA ATCCATAAGC
  721 TCCATGGATT CCTCCCTATT CAGCACTTTG TCCATTTTAG CTTTTTGCAG CAAAAAATTA
  781 CTGCAAAAAA GGGAAAAACA AGGGAATTTC CCTGGCCTCC TAAAAAGCCT CCACGCCCTT
  841 ACTACTTCTG AGTAAGCTTG GAGGCGGAGG CGGCCTCGGC CTCCTGTATA TATAAAAAAA
  901 AGGGAAGGTA GGGAGGAGCT GGCTAAAACT GGATGGCTGC CAGCCAAGCA TGAGCTCATA
  961 CCTAGGGAGC CAACCAGCTG ACAATAGTCC TTAATCACAA AGGAGCTGGG CTAAAACTGG
 1021 ATGGCTGCCA GCCCAAGCAT GAGCTCATAC CTAGGGGAGC CAACAGCCTG GACATAGTCC
1081 TTTAATCACA GTTACTAAGC ACACGGGAAG TGAAGCAGCC AAGGAACCAA TGCTTTTTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447.

2018.12.10

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