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pNY-1B

Complete genome of JC virus, PML-type

Catalog number RDB07251
Resource name pNY-1B
Clone info. Complete genome of JC virus, PML-type. JCV genomic DNA derived from the brain of an American PML patient.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genomic DNA
Reference of nucleotide sequence D12575, D11366 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Virus Genes., 8, 99-105 (1994)).
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RDB07251 pNY-1B DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07251_A3Kjp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2
Region: insert (M13 rev side)
Sequence file: RDB07251_A3Kja.seq check
>07251_07251_B0KrF_Reverse2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTNGN ATGCCTGCAG GTCGACTCTA GAGGANCCTG TGTTTTCATC
   61 ATCACTGGCA AACATTTCTT CATGGCAAAA CAGGTCTTCA TCCCACTTCT CATTAAATGT
  121 ATTCCACCAG GATTCCCATT CATCTGTTCC ATAGGTTGGC ACCTAAAAAA AAACAATTAA
  181 GTTTATTGTA AAAAACAAAA TGCCCTGCAA AAGAAAAATA GTGGTTTACC TTAAAGCTTT
  241 AGATCCCTGT AGGGGGTGTC TCCAAGAACT TTCTCCCAGC AATGAAGAGC TTCTTGGGTT
  301 AAGTCACACC CAAACCATTG TCTGAAGCAA TCAAAGCAAT AGCAATCTAT CCACACAAGT
  361 GGGCTGCTTC TTAAAAATTT TCTGTTTCTA TGCCTTAATT TTAGCATGCA CATTAAACAG
  421 GGGCAATGCA CTGAAGGATT AGTGGCACAG TTAGGCCATT CCTTGCAATA AAGGGTATCA
  481 GAATTAGGAG GAAAATCACA ACCAACCTCT GAACTATTCC ATGTACCAAA ATCAGGCTGA
  541 TGAGCAACTT TTACACCTTG TTCCATTTTT TTATATAAAA AATTCATTCT CTTCATCTTG
  601 TCTTCGTCCC CACCTTTATC AGGGTGGANT TCTTTGCATT TTTTCANATA AGCTTTTCTC
661 ATGACAGGNA TGNTCCCNCN NTGCAGANCT ATCNNNGNNN NNNNNNN
//
Primer: JCV-H_F
Region: insert (D12575.1)
Sequence file: RDB07251_A3Kjb.seq check
>07251_07251_B0KrF_JCV-H_F_G02_20_ABI24.ab1
    1 NNNNNNNTNN NNNNNNANNN NNNTNNTTCT CTTCNTCTTG TCTTCGTCCC CACCTTTATC
   61 AGGGTGGAGT TCTTTGCATT TTTTCAGATA AGCTTTTCTC ATGACAGGAA TGTTCCCCCA
  121 TGCAGACCTA TCAAGGCCTA ATAAATCCAT AAGCTCCATG GATTCCTCCC TATTCAGCAC
  181 TTTGTCCATT TTAGCTTTTT GCAGCAAAAA ATTACTGCAA AAAAGGGAAA AACAAGGGAA
  241 TTTCCCTGGC CTCCTAAAAA GCCTCCACGC CCTTACTACT TCTGAGTAAG CTTGGAGGCG
  301 GAGGCGGCCT CGGCCTCCTG TATATATAAA AAAAAGGGAA GGTAGGGAGG AGCTGGCTAA
  361 AACTGGATGG CTGCCAGCCA AGCATGAGCT CATACCTAGG GAGCCAACCA GCTGACAATA
  421 GTCCTTAATC ACAAGGAGCT GGCTAAAACT GGATGGCTGC CAGCCAAGCA TGAGCTCATA
  481 CCTAGGGAGC CAACCAGCTG ACAATAGTCC TTAATCACAA GTAAACAAAG CACAAGGGGA
  541 AGTGGAAAGC AGCCAAGGGA ACATGTTTTG CGAGCCAGAG CTGTTTTGGC TTGTCACCAG
  601 CTGGCCATGG TTCTTCGCCN GCTGTCACGT AAGGCTTCTG TGAAAGTTAG TAAAACCTGN
  661 ANTGNAACTA AAAAAANANC TCNNNGANTT TNNTTTTTTN GNNANANNTT TTGNNNGNNN
721 TTTNNNNNNN NNN
//
Primer: JCV-M_R1
Region: insert (D11366.1 5')
Sequence file: RDB07251_A3Kjc.seq check
>07251_07251_B0KrF_JCV-M_R1_G01_07_ABI08.ab1
    1 NNNNNNNNCN NNNNNTNGNG NNNNNNNCTT TNNNATGTAT CTGANATAGA TATTGACTTA
   61 CTAAAACCCC TAAGATGCTC ATCTGGGTCA CCCATTTCTG GAGTTAAAAA GCATTCTACC
  121 TCTGTAATTG AGTCAACCCC AGTTTTAACT TCTAGAACTT CTACTCCTCC TCTTATAAGA
  181 AGTTTTGGAA CTTGCACGGG GTCCTTCCTT TCTCCTTTTC TTTTTGTTGG GGCCATCTTC
  241 ATATGCTTCA AGAGCAGGTG TTACAGTCCC GTACAACCCT AAAAGTAAAG GCAACATCCA
  301 TTGAGGAGCA GATCTTTGAT TTGCACCTCC TGGTGCAATA CTTCTTTCTA TAAATTCACC
  361 AGATTGCACA TTTGGGGTTT TTAGATCTAG CCTTTGGGTA ACTTCTTGAA TGCTGTCTGC
  421 ATCATCTATA CTTTGGGTGT ATGAGTGGCC AAAAGAAATA TAGGTTCCCT CCCTTTGGGC
  481 AACTTGCCTT ACCATAGAGG GCCTAACTGG AGACAATCTA GAATAATAGT CTGAAATATA
  541 ATTATATAAG TTAGCTGGTG AATTAACTAT TGCCCAAGTA GTTTCTTCCA AAAACCTTGC
  601 TAAATTTTCA ACAAATAGCT TTTGGGTTCT TCTCTGAATT TCCTGAGANG NTNANNNTGG
  661 CAAATCATCT CTAANNAGAT TCCNAAAANN CNNNNNANTG NGNANAANNA NGGNNNGNNC
721 CNNNNGNNNN NNNNNN
//
Primer: M13_-40
Region: insert (M13 fwd side)
Sequence file: RDB07251_A3Kjd.seq check
>07251_07251_B0KrF_M13_-40_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNCNCNGNNN CCGGGGNNCC CNACACTCTA CCCCACCTAA GAAGAAAAAA
   61 AAGGTAGAAG ACCCTAAAGA CTTTCCTGTA GATCTGCATG CATTCCTCAG TCAAGCTGTG
  121 TTTAGTAATA GAACTGTTGC TTCTTTTGCT GTGTATACCA CTAAAGAAAA AGCTCAAATT
  181 TTATATAAGA AACTTATGGA AAAATATTCT GTAACTTTTA TAAGTAGACA TGGTTTTGGG
  241 GGTCATAATA TTTTGTTTTT CTTAACACCA CATAGACATA GAGTGTCAGC AATTAATAAC
  301 TACTGTCAAA AACTATGTAC CTTTAGTTTT TTAATTTGTA AAGGTGTGAA TAAGGAATAC
  361 TTGTTTTATA GTGCCCTGTG TAGACAGCCA TATGCAGTAG TGGAAGAAAG TATTCAGGGG
  421 GGCCTTAAGG AGCATGACTT TAACCCAGAA GAACCAGAAG AAACTAAGCA GGTTTCATGG
  481 AAATTAGTTA CACAGTATGC CTTGGAAACC AAGTGTGAGG ATGTTTTTTT GCTTATGGGC
  541 ATGTACTTAG ACTTTCAGGA AAACCCACAG CAATGCAAAA AATGTGAAAA AAAGGATCAG
  601 CCAAATCACT TTAACNATCA TGAAAAACAC TATTATAATG CCCAAATTTT TGCAGANAGC
  661 AAAAATCNAA AAAGCANTTG NNNGCNNNCT GTTGANNCTG NANCAGCCAA ACAAANGGTT
721 GANNNNATCC NCANNNNNNN NNN
//
Primer: JCV-M_F3
Region: insert (D11366.1 5')
Sequence file: RDB07251_A3Kje.seq check
>07251_07251_B0KrF_JCV-M_F3_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN TTCTAGAAGT TAAAACTGGG GTTGACTCAA TTACAGAGGT
   61 AGAATGCTTT TTAACTCCAG AAATGGGTGA CCCAGATGAG CATCTTAGGG GTTTTAGTAA
  121 GTCAATATCT ATATCAGATA CATTTGAAAG TGACTCCCCA AGTAAGGACA TGCTTCCTTG
  181 TTACAGTGTG GCCAGAATTC CACTACCCAA TCTAAATGAG GATCTAACCT GTGGAAATAT
  241 ACTCATGTGG GAGGCTGTGA CCTTAAAAAC TGAGGTTATA GGGGTGACAA GTTTGATGAA
  301 TGTGCACTCT AATGGTCAAG CAGCTCATGA CAATGGTGCA GGGAAGCCAG TGCAGGGCAC
  361 CAGCTTTCAT TTTTTTTCTG TTGGGGGGGA GGCTTTAGAA TTACAGGGGG TGGTTTTTAA
  421 TTACAGAACA AAGTACCCAG ATGGAACAAT TTTTCCNAAG AANGCCACAG TGCAATCTCA
  481 AGTCATGAAC ACAGAGCACA AGGCGTACCT AGATAAGAAC AAAGCATATC CTGNTGAATG
  541 NTGGNTNNNN GATNCCNCCN GANATGAANA CNNNNGANAT TTNGGGNNNC TNNNNNNNNN
601 ANNAAANGNN NNNNNNNN
//
Primer: JCV-M_R3
Region: insert (D11366.1 mid)
Sequence file: RDB07251_A3Kjf.seq check
>07251_07251_B0KrF_JCV-M_R3_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNNNNNGNNT CCCCAAGTAA GGACATGCTT CCTTGTTACA
   61 GTGTGGCCAG AATTCCACTA CCCAATCTAA ATGAGGATCT AACCTGTGGA AATATACTCA
  121 TGTGGGAGGC TGTGACCTTA AAAACTGAGG TTATAGGGGT GACAAGTTTG ATGAATGTGC
  181 ACTCTAATGG TCAAGCAGCT CATGACAATG GTGCAGGGAA GCCAGTGCAG GGCACCAGCT
  241 TTCATTTTTT TTCTGTTGGG GGGGAGGCTT TAGAATTACA GGGGGTGGTT TTTAATTACA
  301 GAACAAAGTA CCCAGATGGA ACAATTTTTC CAAAGAATGC CACAGTGCAA TCTCAAGTCA
  361 TGAACACAGA GCACAAGGCG TACCTAGATA AGAACAAAGC ATATCCTGTT GAATGTTGGG
  421 TTCCTGATCC CACCAGAAAT GAAAACACAA GATATTTTGG GACACTAACA GGAGGAGAAA
  481 ATGTTCCTCC AGTTCTTCAT ATAACAAACA CTGCCACAAC AGTGTTGCTT GATGAATTTG
  541 GTGTTGGGCC ACTTTGCAAA GGTGACAACT TATACTTGTC AGCTGTTGAT GTCTGTGGCA
  601 TGTTTACTAA CAGGTCTGGT TCCCAGCAGT GNANAGGACT GTCCAGATAT TTTAANGTTC
  661 AGCTNANNNA AANGANGGTT AAAAACCCNN ACCNNATTTC TTTNNNNCNN ACTNNNTNNN
721 NTNNNNNNNN NN
//
Primer: JCV-B_R2
Region: insert (D11366.1 3')
Sequence file: RDB07251_A3Kjg.seq check
>07251_07251_B0KrF_JCV-B_R2_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNAANNTGTA NNNNNTATTA CAATAAAGGC TTTTGATTAC AGCATTTTTG
   61 TCTGCAACTG TCCATATTTG TCAACATATC TCATCATGTC TGGGTCCCCT GGAAGCTCCT
  121 CTGTTCCCTC AAAAACTCTA ACCTCCTCTA CTTGAGCATC CATGCCATAC ATAGGCTGCC
  181 CATCAACTCT AGGAGTCCTT CTGTTAATTA AATCAGTAAG AAGGAAAGAA ATTGGGTAGG
  241 GGTTTTTAAC CCTCCTTTTC CTTAGCTGAA CCTTAAAATA TCTGGACAGT CCTCTCCACT
  301 GCTGGGAACC AGACCTGTTA GTAAACATGC CACAGACATC AACAGCTGAC AAGTATAAGT
  361 TGTCACCTTT GCAAAGTGGC CCAACACCAA ATTCATCAAG CAACACTGTT GTGGCAGTGT
  421 TTGTTATATG AAGAACTGGA GGAACATTTT CTCCTCCTGT TAGTGTCCCA AAATATCTTG
  481 TGTTTTCATT TCTGGTGGGA TCAGGAACCC AACATTCAAC AGGATATGCT TTGTTCTTAT
  541 CTAGGTACGC CTTGTGCTCT GTGTTCATGA CTTGAGNNTG CACTGTGGCA TTCTTTGGAA
  601 AAATTGTTCC ATCTGGGTAC TTTGTTCTGT AATTAAAAAC CACCCCCTGT AATTCTAAAG
661 CCTCCCCCCC CNANAGAAAA AAAANGNANN CNNGNGNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447.

2020.12.22

GNP_filter3_RDBDEP_html_201222.pl