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pBRPyCAG-cHA-IN

Expression vector with CAG promoter, HA-tag, Neomycin selection.

Catalog number RDB07105
Resource name pBRPyCAG-cHA-IN
Alternative name pCAG-IN, stock no 1994
Clone info. Expression vector with CAG promoter, HA-tag, Neomycin selection.
Vector backbone pBRPyCAG-cHA-IN (Plasmid)
Selectable markers Amp^r (E. coli); neomycin (mammalian cells)
Gene/insert name cDNA
Depositor|Developer Niwa, Hitoshi |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB07105 pBRPyCAG-cHA-IN DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB07105z.seq
            Remarks, protocol and/or map (gif) RDB07105a.gif
            Remarks, protocol and/or map (pdf) RDB07105p.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB10973_A4L1p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB10973_A4L1a.seq check
            >07105_10973_A4L1_2_pAxCA_F1_A02_02.ab1
                1 CATTGAGCAC GGCGGGGGTT CTCTGCTACA TGTTTCTTGC CTTCTTCTTT TTCCTACAGC
               61 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG
              121 ATCTCACGCG TGACTAGTGC CATGGCCTAC CCATACGACG TGCCAGACTA CGCCTAGCGG
            181 CCGCTCGATA AGCTTGATAT CGAATTCCGC CCCCCCCCCC TCTCCCT
            //
            Primer: IRES-F
            Sequence file: RDB10973_A4L1b.seq check
            >07105_10973_A4L1_2_IRES-F_B02_05.ab1
                1 GCCTGTCCCT TAAATGTGTT TTAGGTCGGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC
               61 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGATTGAA
              121 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC
              181 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG
              241 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG
              301 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT
              361 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG
              421 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG
              481 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA
              541 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG
              601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT
              661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
              721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG
              781 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT
              841 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC
              901 TTCTGAGGGG ATCGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT
              961 CGGATCCCAC GGGTGCGCAT GATTCGTGCT CCCTGTCGTT GAGGACCCGG GCTAGGCTGG
             1021 CGGGGGGTTG CCCTTACTGG GTTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA
             1081 AGCGACTGCC TGCTGCAAAA CGTCTGCCGA ACCTGAACAA CCACAATGAA TGATCTTCCG
             1141 GGATTACGTT GTATTTCCGT AAAGTTTCTT GGAAACACGG CGGGAAAAGT TTCTCAGGTA
            1201 G
            //
            Primer: SV40pA3ElementReverse
            Sequence file: RDB10973_A4L1c.seq check
            >07105_10973_A4L1_2_SV40pA3ElementReverse_C02_08.ab1
                1 GACGTCGGGG GGGGGGCGGA GCCTATGGAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
               61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
              121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
              181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG
              241 CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC
              301 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC
              361 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT
              421 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC
              481 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA
              541 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC
              601 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG
              661 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA
              721 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC
              781 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG
              841 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA
              901 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA
              961 AGACCATTCA TGTTGTTGCT CAAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT
             1021 CGCTCGCGTA TCGGGTGATT CATTTCTGCT AACCAGTAAA GGCAACCCCG CCCAGCCCTA
             1081 GCCCGGTTCC TCAACGACAA GGGAGCACGG AATCATGCGC AACCGTTGGA TCCCTAAACA
            1141 AAACGAACCC AACCACCCCG CGGTGCGCGG TGT
            //
            Primer: Amp_R
            Sequence file: RDB10973_A4L1d.seq check
            >07105_10973_A4L1_2_Amp_R_E09_15.ab1
                1 ATGTCGTTTT TATGACCGCT TTGGAAAACA AAGACTGTAT TTCCTGGAAA TTAATGTTTA
               61 TTCAATAAAC TGTGTATTCA GCTATATTCA CATAGTGGTG AGGCTGAAAT GAGGCGGGAA
              121 GAGGCGGTTG GGGCTTAATT ATATCAATTT GGGTGGCCCC ACAGCGCCTC CAAGGCGCCA
              181 GTCCTGTTTT GACAAGTTGC CTCTGGAAGC CTCTCTTCTT TTTCTCCAGA GTAAGCGGAG
              241 GCCAGGGGCC CCCGGCCTCT GCTTAATACT AAAAAAAACA GCTGTTGTCA TAGTAATGAT
              301 TGGGTGGAAA CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA
              361 CTGACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGCCC
              421 TCTGGAGGGC AGTTGCCTAG CAACTAATTA AAAGAGGATG TCGCACGGCC AGCTGCGGTC
              481 AGTTAGTCAC TTCCTGCTTA ACTGACTTGA CATTTTCTAT TTTAAGAGTC GGGAGGAAAA
              541 TTACTGTGTT GGAGGCCCTC CGCCATCTTC TGAAGCTGAA TCGAATTAAC TTGTTTATTG
              601 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
              661 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA
              721 TCTGATATCA TCGTCGACAT TGATTATTGA CTAGCTAGTT ATTAATAGTA ATCAATTACG
              781 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
              841 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
              901 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
              961 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
             1021 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCCTTATGGG ACTTTCCTAC
             1081 TTGGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GATGTCGAAG TTGAGCCCAC
            1141 GTCCTGCCTC CACTCTCCCC ATTCTTCCCC CCCCCTTTCC CCAAAGGC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl