Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-cHA-IN (#RDB07105)

Expression vector with CAG promoter, HA-tag, Neomycin selection.

Alternative name	pCAG-IN, stock no 1994
Clone info.	Expression vector with CAG promoter, HA-tag, Neomycin selection.
Vector backbone	pBRPyCAG-cHA-IN (Plasmid)
Selectable markers	Amp^r (E. coli); neomycin (mammalian cells)
Gene/insert name	cDNA
Depositor|Developer	Niwa, Hitoshi |

 

Sequence	RDB07105z.seq
Additional map
Remarks, protocol and/or map (pdf)	RDB07105p.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07105	pBRPyCAG-cHA-IN	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pBRPyCAG-cHA-IN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07105).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB10973_A4L1p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 () Sequence file: RDB10973_A4L1a.seq
>07105_10973_A4L1_2_pAxCA_F1_A02_02.ab1     1 CATTGAGCAC GGCGGGGGTT CTCTGCTACA TGTTTCTTGC CTTCTTCTTT TTCCTACAGC    61 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG   121 ATCTCACGCG TGACTAGTGC CATGGCCTAC CCATACGACG TGCCAGACTA CGCCTAGCGG 181 CCGCTCGATA AGCTTGATAT CGAATTCCGC CCCCCCCCCC TCTCCCT //
Primer: IRES-F () Sequence file: RDB10973_A4L1b.seq
>07105_10973_A4L1_2_IRES-F_B02_05.ab1     1 GCCTGTCCCT TAAATGTGTT TTAGGTCGGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC    61 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGATTGAA   121 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC   181 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG   241 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG   301 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT   361 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG   421 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG   481 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA   541 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG   601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT   661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT   721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG   781 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT   841 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC   901 TTCTGAGGGG ATCGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT   961 CGGATCCCAC GGGTGCGCAT GATTCGTGCT CCCTGTCGTT GAGGACCCGG GCTAGGCTGG  1021 CGGGGGGTTG CCCTTACTGG GTTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA  1081 AGCGACTGCC TGCTGCAAAA CGTCTGCCGA ACCTGAACAA CCACAATGAA TGATCTTCCG  1141 GGATTACGTT GTATTTCCGT AAAGTTTCTT GGAAACACGG CGGGAAAAGT TTCTCAGGTA 1201 G //
Primer: SV40pA3ElementReverse () Sequence file: RDB10973_A4L1c.seq
>07105_10973_A4L1_2_SV40pA3ElementReverse_C02_08.ab1     1 GACGTCGGGG GGGGGGCGGA GCCTATGGAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC    61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG   121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG   181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG   241 CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC   301 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC   361 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT   421 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC   481 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA   541 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC   601 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG   661 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA   721 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC   781 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG   841 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA   901 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA   961 AGACCATTCA TGTTGTTGCT CAAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT  1021 CGCTCGCGTA TCGGGTGATT CATTTCTGCT AACCAGTAAA GGCAACCCCG CCCAGCCCTA  1081 GCCCGGTTCC TCAACGACAA GGGAGCACGG AATCATGCGC AACCGTTGGA TCCCTAAACA 1141 AAACGAACCC AACCACCCCG CGGTGCGCGG TGT //
Primer: Amp_R () Sequence file: RDB10973_A4L1d.seq
>07105_10973_A4L1_2_Amp_R_E09_15.ab1     1 ATGTCGTTTT TATGACCGCT TTGGAAAACA AAGACTGTAT TTCCTGGAAA TTAATGTTTA    61 TTCAATAAAC TGTGTATTCA GCTATATTCA CATAGTGGTG AGGCTGAAAT GAGGCGGGAA   121 GAGGCGGTTG GGGCTTAATT ATATCAATTT GGGTGGCCCC ACAGCGCCTC CAAGGCGCCA   181 GTCCTGTTTT GACAAGTTGC CTCTGGAAGC CTCTCTTCTT TTTCTCCAGA GTAAGCGGAG   241 GCCAGGGGCC CCCGGCCTCT GCTTAATACT AAAAAAAACA GCTGTTGTCA TAGTAATGAT   301 TGGGTGGAAA CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA   361 CTGACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGCCC   421 TCTGGAGGGC AGTTGCCTAG CAACTAATTA AAAGAGGATG TCGCACGGCC AGCTGCGGTC   481 AGTTAGTCAC TTCCTGCTTA ACTGACTTGA CATTTTCTAT TTTAAGAGTC GGGAGGAAAA   541 TTACTGTGTT GGAGGCCCTC CGCCATCTTC TGAAGCTGAA TCGAATTAAC TTGTTTATTG   601 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT   661 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA   721 TCTGATATCA TCGTCGACAT TGATTATTGA CTAGCTAGTT ATTAATAGTA ATCAATTACG   781 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC   841 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC   901 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT   961 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT  1021 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCCTTATGGG ACTTTCCTAC  1081 TTGGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GATGTCGAAG TTGAGCCCAC 1141 GTCCTGCCTC CACTCTCCCC ATTCTTCCCC CCCCCTTTCC CCAAAGGC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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