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pBRPyCAG-cHA-IN

Expression vector with CAG promoter, HA-tag, Neomycin selection.

Catalog number RDB07105
Resource name pBRPyCAG-cHA-IN
Alternative name pCAG-IN, stock no 1994
Clone info. Expression vector with CAG promoter, HA-tag, Neomycin selection.
Vector backbone pBRPyCAG-cHA-IN (Plasmid)
Selectable markers Amp^r (E. coli); neomycin (mammalian cells)
Gene/insert name cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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RDB07105 pBRPyCAG-cHA-IN DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07105z.seq from Depositor
Electronic file Map RDB07105a.gif from Depositor
Electronic file PDF (map, protocol and/or remarks) RDB07105p.pdf from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB10973_A4L1p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB10973_A4L1a.seq
>07105_10973_A4L1_2_pAxCA_F1_A02_02.ab1
    1 CATTGAGCAC GGCGGGGGTT CTCTGCTACA TGTTTCTTGC CTTCTTCTTT TTCCTACAGC
   61 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG
  121 ATCTCACGCG TGACTAGTGC CATGGCCTAC CCATACGACG TGCCAGACTA CGCCTAGCGG
181 CCGCTCGATA AGCTTGATAT CGAATTCCGC CCCCCCCCCC TCTCCCT
//
Primer: IRES-F RDB10973_A4L1b.seq
>07105_10973_A4L1_2_IRES-F_B02_05.ab1
    1 GCCTGTCCCT TAAATGTGTT TTAGGTCGGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC
   61 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGATTGAA
  121 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC
  181 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG
  241 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG
  301 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT
  361 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG
  421 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG
  481 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA
  541 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG
  601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT
  661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
  721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG
  781 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT
  841 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC
  901 TTCTGAGGGG ATCGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT
  961 CGGATCCCAC GGGTGCGCAT GATTCGTGCT CCCTGTCGTT GAGGACCCGG GCTAGGCTGG
 1021 CGGGGGGTTG CCCTTACTGG GTTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA
 1081 AGCGACTGCC TGCTGCAAAA CGTCTGCCGA ACCTGAACAA CCACAATGAA TGATCTTCCG
 1141 GGATTACGTT GTATTTCCGT AAAGTTTCTT GGAAACACGG CGGGAAAAGT TTCTCAGGTA
1201 G
//
Primer: SV40pA3ElementReverse RDB10973_A4L1c.seq
>07105_10973_A4L1_2_SV40pA3ElementReverse_C02_08.ab1
    1 GACGTCGGGG GGGGGGCGGA GCCTATGGAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
   61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
  121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
  181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG
  241 CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC
  301 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC
  361 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT
  421 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC
  481 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA
  541 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC
  601 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG
  661 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA
  721 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC
  781 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG
  841 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA
  901 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA
  961 AGACCATTCA TGTTGTTGCT CAAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT
 1021 CGCTCGCGTA TCGGGTGATT CATTTCTGCT AACCAGTAAA GGCAACCCCG CCCAGCCCTA
 1081 GCCCGGTTCC TCAACGACAA GGGAGCACGG AATCATGCGC AACCGTTGGA TCCCTAAACA
1141 AAACGAACCC AACCACCCCG CGGTGCGCGG TGT
//
Primer: Amp_R RDB10973_A4L1d.seq
>07105_10973_A4L1_2_Amp_R_E09_15.ab1
    1 ATGTCGTTTT TATGACCGCT TTGGAAAACA AAGACTGTAT TTCCTGGAAA TTAATGTTTA
   61 TTCAATAAAC TGTGTATTCA GCTATATTCA CATAGTGGTG AGGCTGAAAT GAGGCGGGAA
  121 GAGGCGGTTG GGGCTTAATT ATATCAATTT GGGTGGCCCC ACAGCGCCTC CAAGGCGCCA
  181 GTCCTGTTTT GACAAGTTGC CTCTGGAAGC CTCTCTTCTT TTTCTCCAGA GTAAGCGGAG
  241 GCCAGGGGCC CCCGGCCTCT GCTTAATACT AAAAAAAACA GCTGTTGTCA TAGTAATGAT
  301 TGGGTGGAAA CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA
  361 CTGACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGCCC
  421 TCTGGAGGGC AGTTGCCTAG CAACTAATTA AAAGAGGATG TCGCACGGCC AGCTGCGGTC
  481 AGTTAGTCAC TTCCTGCTTA ACTGACTTGA CATTTTCTAT TTTAAGAGTC GGGAGGAAAA
  541 TTACTGTGTT GGAGGCCCTC CGCCATCTTC TGAAGCTGAA TCGAATTAAC TTGTTTATTG
  601 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
  661 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA
  721 TCTGATATCA TCGTCGACAT TGATTATTGA CTAGCTAGTT ATTAATAGTA ATCAATTACG
  781 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
  841 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
  901 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
  961 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
 1021 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCCTTATGGG ACTTTCCTAC
 1081 TTGGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GATGTCGAAG TTGAGCCCAC
1141 GTCCTGCCTC CACTCTCCCC ATTCTTCCCC CCCCCTTTCC CCAAAGGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.05.29

GNP_filter3_RDBDEP_html_190430.pl