Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-cHA-IP

Expression vector with CAG promoter, HA-tag, Puromycin selection.

Sequence (full)	RDB07100zzk02.seq provided by the depositor
Remarks, protocol and/or map (gif)	RDB07100a.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 22, 1526-1536 (2002))。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07100_A5Edp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Region: CAG pro,HA,IRES 5' Sequence file: RDB07100_A5Edd.seq
>07100_07100_B1BjS_pAxCA_F1_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC CTTCTTCTTT TTCCTACNGC TCCTGGGCAA CGTGCTGGTT    61 ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGA TCTCACGCGT GACTAGTGCC   121 ATGGCCTACC CATACGACGT GCCAGACTAC GCCTAGCGGC CGCTCGATAA GCTTGATATC   181 GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT   241 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG   301 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC   361 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA   421 AGCTTCTNGN NNACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC   481 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGNATAA NATACACCTG CAAAGGCGGC   541 ACNNNCCNCN NNGCCACGTT GTGAGTTNNG ATAGTTGTGG AAAGAGTCNA NTGGCTCTCN   601 NCAAGCGTAN TCNNNNAGGG GCTGAAGGNN NNCCNNNNNN NNCCCNTTGT NNGGGATCTG   661 ATNNGGNNCC TNNNNGNNNN NGNNTNACNN GTGTNANNNN NNNNNAAAAA ANNTCTANGN 721 CNNCNNNNNN NNNN //
Primer: Reverse-2 Region: bGHpA,PuroR Sequence file: RDB07100_A5Ede.seq
>07100_07100_A5Ed_Reverse2_E06_15.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNGGCTGC AGGTTCTTTC CGCCTCNGAA GCCATAGAGC    61 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC   121 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT   181 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG   241 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGAGCTCTAG   301 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG   361 CGTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG   421 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC   481 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG   541 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG   601 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG   661 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG   721 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG   781 ATGTCGAGCC CGANGCGCGT GANGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG   841 CGGTCCGGAT CGACGGTGTG GCGCGTGGCG GGGTANTCGG CGAACGCGGC GGCNANGGNG   901 CGTACNGNCC NNGGGNNNNC GNCNCGGGNG GCGAGGCGCA CCNNGGGCTT GNACTCGGNC   961 ANNGNNNGNG GNCNATNTTA TCNTCGNGNT TTNCNANNNA AAANNNNNTC CCNNNNGNNN 1021 NNGGGGGNNN NNNNNNN //
Primer: Amp_R Region: Polyoma ori,SV40 pA,CMV en Sequence file: RDB07100_A5Edf.seq
>07100_07100_A9DnF_Amp_R_C10_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNGCTT TNGNNNNNNA GANTGTATTT CCTGGAAATT AATGTTTATT    61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA   121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT   181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC   241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG   301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT   361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC   421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG   481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT   541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA   601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT   661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC   721 TGATATCATC GTCGACATTG ATTATTGACT AGCTAGTTAT TAATAGTAAT CAATTACGGG   781 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACNG TAAATGGNCC   841 GCCTGGCTGA NCGCCCNACG ACCCCCGCCC ATTGACGTCN ATAATGACGT ATGTTCCNNT   901 AGTNACGNCN ATAGGGACTT TCNNTTGNCG TCANTGGGNG GANTATTTAN NNNNAACTGN   961 CNACTTNGCA NTACATNNNG NGTATNNNNT GNCNNNNTAN GCCCCCNNNT NNNGNNNNNN 1021 NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl