Expression vector with CAG promoter, HA-tag, Puromycin selection.
| Catalog number | RDB07100 |
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| Resource name | pPyCAG-cHA-IP |
| Alternative name | pCAGIP, pPyCAG-cHA-IP, stock no 1426 |
| Clone info. | Expression vector with CAG promoter, HA-tag, SV40 poly(A) and polyoma ori. Puromycin selection. |
| Vector backbone | Plasmid |
| Selectable markers | Amp^r (E. coli), Puro^r (mammalian cells) |
| Gene/insert name | cDNA |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002)) |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB07100 | pPyCAG-cHA-IP | DNA solution |
Electronic file
| Electronic file | Sequence RDB07100z.seq from Depositor |
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| Electronic file | Map RDB07100a.gif from Depositor
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB07100_A5Edp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB07100_A5Eda.seq |
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>07100_07100_A5Ed_pAxCA_F1_D06_12.ab1 1 CCAGGCGCGG GGGGGTCTTT CTACATGTTA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG 61 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGATCTCACG 121 CGTGACTAGT GCCATGGCCT ACCCATACGA CGTGCCAGAC TACGCCTAGC GGCCGCTCGA 181 TAAGCTTGAT ATCGAATTCC GCCCCCCCCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG 241 GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT 301 TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC 361 CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG 421 CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC 481 GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC 541 CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA 601 AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT 661 GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA 721 AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT TCCTTTTGAA AAAACACGAT 781 GATAATATGG GCCTGCAACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG 841 ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC 901 GCCACACCGT CCAATCCGGG ACCGTCACAT CGAGCGGGGT CCACCGAGCT GGCAAGAACT 961 CTTTCTTCAC GCGCGTCGGG CTTCGACAAT CGGCAAGGTG TGGGTCGCGG AACGACGGCG 1021 CCGCGGTGAC GGTCTAGGAC CACGCCCGGA AAAGCGTCGA AGCGGGGGGC GGTGTTCGCT 1081 CGAAATCGGT CCCCCCGCAA TGGTCCG // |
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| Primer: Reverse2 | RDB07100_A5Edb.seq |
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>07100_07100_A5Ed_Reverse2_E06_15.ab1 1 GGGGACATGA ATTCGGCCAG CTTGGGCTGC AGGTTCTTTC CGCCTCAGAA GCCATAGAGC 61 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC 121 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT 181 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG 241 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGAGCTCTAG 301 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG 361 CGTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG 421 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC 481 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG 541 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG 601 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG 661 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG 721 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG 781 ATGTCGAGCC CGACGCGCGT GAGGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG 841 CGGTCCGGAT CGACGGTGTG GCGCGTGGCG GGGTAGTCGG CGAACGCGGC GGCGAGGGTG 901 CGTACGGCCC TGGGGACGTC GTCGCGGGTG GCGAGGCGCA CCGTGGGCTT GTACTCGGTC 961 ATGGGTTGTG GGCCATATTA TCATCGTGTT TTTCAAAGGA AAACCACGTC CCCGTGGGTT 1021 CGGGGGGGCC TAGACGTTTT TTTTAACCTC GACTAAACAC ATGTAAGCAT GTGCACCGAG 1081 GCCCCAGATC AGATCCATAC AATGGGGGTA CCTCTGGCAA TCTCCAGCTC CCTTTGATGG 1141 ATTACGCTTG GAGGGAAGAA GCCAATTTTT GAACTCT // |
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| Primer: Amp_R | RDB07100_A5Edc.seq |
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>07100_07100_A9DnF_Amp_R_C10_07_ABI24.ab1 1 CTTCGTTTTC ATGACCGCTT TGGAAACAAA GACTGTATTT CCTGGAAATT AATGTTTATT 61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA 121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT 181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC 241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG 301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT 361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC 421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG 481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT 541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA 601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC 721 TGATATCATC GTCGACATTG ATTATTGACT AGCTAGTTAT TAATAGTAAT CAATTACGGG 781 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 841 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 901 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 961 CCACTTGGCA GTACATCAAG TGTATCATAT GCCCAAGTAC GCCCCCTATT GACGTCAATG 1021 ACGGTAAATG GGCCGCCTGG GCATTTATGC CCAGTACATG ACGTTATGGG ACTTTCCTAC 1081 CTGCAGTACA TCTACGTATT AGGTCAATCG CTATTACAAT GGAATGTTCG AAGGTGGAGC 1141 CCCCCAACGT TCTGTGCCTT TCCACTTCTC TTCATCA // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.05.13
GNP_filter3_RDBDEP_html_190430.pl