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pPyCAG-cHA-IP

Expression vector with CAG promoter, HA-tag, Puromycin selection.

Catalog number RDB07100
Resource name pPyCAG-cHA-IP
Alternative name pCAGIP, pPyCAG-cHA-IP, stock no 1426
Clone info. Expression vector with CAG promoter, HA-tag, SV40 poly(A) and polyoma ori. Puromycin selection.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells)
Gene/insert name cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
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RDB07100 pPyCAG-cHA-IP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07100z.seq from Depositor
Electronic file Map RDB07100a.gif from Depositor

Original reference

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818 .

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07100_A5Edp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB07100_A5Eda.seq
>07100_07100_A5Ed_pAxCA_F1_D06_12.ab1
    1 CCAGGCGCGG GGGGGTCTTT CTACATGTTA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
   61 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGATCTCACG
  121 CGTGACTAGT GCCATGGCCT ACCCATACGA CGTGCCAGAC TACGCCTAGC GGCCGCTCGA
  181 TAAGCTTGAT ATCGAATTCC GCCCCCCCCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG
  241 GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT
  301 TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC
  361 CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG
  421 CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC
  481 GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC
  541 CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA
  601 AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT
  661 GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA
  721 AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT TCCTTTTGAA AAAACACGAT
  781 GATAATATGG GCCTGCAACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG
  841 ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC
  901 GCCACACCGT CCAATCCGGG ACCGTCACAT CGAGCGGGGT CCACCGAGCT GGCAAGAACT
  961 CTTTCTTCAC GCGCGTCGGG CTTCGACAAT CGGCAAGGTG TGGGTCGCGG AACGACGGCG
 1021 CCGCGGTGAC GGTCTAGGAC CACGCCCGGA AAAGCGTCGA AGCGGGGGGC GGTGTTCGCT
1081 CGAAATCGGT CCCCCCGCAA TGGTCCG
//
Primer: Reverse2 RDB07100_A5Edb.seq
>07100_07100_A5Ed_Reverse2_E06_15.ab1
    1 GGGGACATGA ATTCGGCCAG CTTGGGCTGC AGGTTCTTTC CGCCTCAGAA GCCATAGAGC
   61 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC
  121 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT
  181 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG
  241 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGAGCTCTAG
  301 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
  361 CGTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG
  421 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC
  481 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG
  541 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG
  601 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG
  661 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG
  721 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG
  781 ATGTCGAGCC CGACGCGCGT GAGGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG
  841 CGGTCCGGAT CGACGGTGTG GCGCGTGGCG GGGTAGTCGG CGAACGCGGC GGCGAGGGTG
  901 CGTACGGCCC TGGGGACGTC GTCGCGGGTG GCGAGGCGCA CCGTGGGCTT GTACTCGGTC
  961 ATGGGTTGTG GGCCATATTA TCATCGTGTT TTTCAAAGGA AAACCACGTC CCCGTGGGTT
 1021 CGGGGGGGCC TAGACGTTTT TTTTAACCTC GACTAAACAC ATGTAAGCAT GTGCACCGAG
 1081 GCCCCAGATC AGATCCATAC AATGGGGGTA CCTCTGGCAA TCTCCAGCTC CCTTTGATGG
1141 ATTACGCTTG GAGGGAAGAA GCCAATTTTT GAACTCT
//
Primer: Amp_R RDB07100_A5Edc.seq
>07100_07100_A9DnF_Amp_R_C10_07_ABI24.ab1
    1 CTTCGTTTTC ATGACCGCTT TGGAAACAAA GACTGTATTT CCTGGAAATT AATGTTTATT
   61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
  121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
  181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
  241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
  301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
  361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
  421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
  481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT
  541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA
  601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC
  721 TGATATCATC GTCGACATTG ATTATTGACT AGCTAGTTAT TAATAGTAAT CAATTACGGG
  781 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
  841 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
  901 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
  961 CCACTTGGCA GTACATCAAG TGTATCATAT GCCCAAGTAC GCCCCCTATT GACGTCAATG
 1021 ACGGTAAATG GGCCGCCTGG GCATTTATGC CCAGTACATG ACGTTATGGG ACTTTCCTAC
 1081 CTGCAGTACA TCTACGTATT AGGTCAATCG CTATTACAAT GGAATGTTCG AAGGTGGAGC
1141 CCCCCAACGT TCTGTGCCTT TCCACTTCTC TTCATCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818 .

2019.05.13

GNP_filter3_RDBDEP_html_190430.pl