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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-cHA-IP

Expression vector with CAG promoter, HA-tag, Puromycin selection.

Catalog number RDB07100
Resource name pPyCAG-cHA-IP
Alternative name pCAGIP, pPyCAG-cHA-IP, stock no 1426
Clone info. Expression vector with CAG promoter, HA-tag, SV40 poly(A) and polyoma ori. Puromycin selection.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells)
Gene/insert name cDNA
Depositor|Developer Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB07100 pPyCAG-cHA-IP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB07100zzk02.seq
Electronic file Map RDB07100a.gif

Original reference

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818 .

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07100_A5Edp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: CAG pro,HA,IRES 5'
Sequence file: RDB07100_A5Edd.seq check
>07100_07100_B1BjS_pAxCA_F1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC CTTCTTCTTT TTCCTACNGC TCCTGGGCAA CGTGCTGGTT
   61 ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGA TCTCACGCGT GACTAGTGCC
  121 ATGGCCTACC CATACGACGT GCCAGACTAC GCCTAGCGGC CGCTCGATAA GCTTGATATC
  181 GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
  241 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
  301 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
  361 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
  421 AGCTTCTNGN NNACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
  481 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGNATAA NATACACCTG CAAAGGCGGC
  541 ACNNNCCNCN NNGCCACGTT GTGAGTTNNG ATAGTTGTGG AAAGAGTCNA NTGGCTCTCN
  601 NCAAGCGTAN TCNNNNAGGG GCTGAAGGNN NNCCNNNNNN NNCCCNTTGT NNGGGATCTG
  661 ATNNGGNNCC TNNNNGNNNN NGNNTNACNN GTGTNANNNN NNNNNAAAAA ANNTCTANGN
721 CNNCNNNNNN NNNN
//
Primer: Reverse-2
Region: bGHpA,PuroR
Sequence file: RDB07100_A5Ede.seq check
>07100_07100_A5Ed_Reverse2_E06_15.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTNGGCTGC AGGTTCTTTC CGCCTCNGAA GCCATAGAGC
   61 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC
  121 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT
  181 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG
  241 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGAGCTCTAG
  301 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
  361 CGTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG
  421 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC
  481 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG
  541 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG
  601 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG
  661 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG
  721 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG
  781 ATGTCGAGCC CGANGCGCGT GANGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG
  841 CGGTCCGGAT CGACGGTGTG GCGCGTGGCG GGGTANTCGG CGAACGCGGC GGCNANGGNG
  901 CGTACNGNCC NNGGGNNNNC GNCNCGGGNG GCGAGGCGCA CCNNGGGCTT GNACTCGGNC
  961 ANNGNNNGNG GNCNATNTTA TCNTCGNGNT TTNCNANNNA AAANNNNNTC CCNNNNGNNN
1021 NNGGGGGNNN NNNNNNN
//
Primer: Amp_R
Region: Polyoma ori,SV40 pA,CMV en
Sequence file: RDB07100_A5Edf.seq check
>07100_07100_A9DnF_Amp_R_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCTT TNGNNNNNNA GANTGTATTT CCTGGAAATT AATGTTTATT
   61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
  121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
  181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
  241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
  301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
  361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
  421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
  481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT
  541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA
  601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC
  721 TGATATCATC GTCGACATTG ATTATTGACT AGCTAGTTAT TAATAGTAAT CAATTACGGG
  781 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACNG TAAATGGNCC
  841 GCCTGGCTGA NCGCCCNACG ACCCCCGCCC ATTGACGTCN ATAATGACGT ATGTTCCNNT
  901 AGTNACGNCN ATAGGGACTT TCNNTTGNCG TCANTGGGNG GANTATTTAN NNNNAACTGN
  961 CNACTTNGCA NTACATNNNG NGTATNNNNT GNCNNNNTAN GCCCCCNNNT NNNGNNNNNN
1021 NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818 .

2021.03.02

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