Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-cHA-IP

Expression vector with CAG promoter, HA-tag, Puromycin selection.

Catalog number RDB07100
Resource name pPyCAG-cHA-IP
Alternative name pCAGIP, pPyCAG-cHA-IP, stock no 1426
Clone info. Expression vector with CAG promoter, HA-tag, SV40 poly(A) and polyoma ori. Puromycin selection.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells)
Gene/insert name cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB07100zzk02.seq provided by the depositor
            Remarks, protocol and/or map (gif) RDB07100a.gif

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 22, 1526-1536 (2002))。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07100 pPyCAG-cHA-IP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818 .

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07100_A5Edp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Region: CAG pro,HA,IRES 5'
            Sequence file: RDB07100_A5Edd.seq check
            >07100_07100_B1BjS_pAxCA_F1_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNC CTTCTTCTTT TTCCTACNGC TCCTGGGCAA CGTGCTGGTT
               61 ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGA TCTCACGCGT GACTAGTGCC
              121 ATGGCCTACC CATACGACGT GCCAGACTAC GCCTAGCGGC CGCTCGATAA GCTTGATATC
              181 GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
              241 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
              301 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
              361 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
              421 AGCTTCTNGN NNACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
              481 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGNATAA NATACACCTG CAAAGGCGGC
              541 ACNNNCCNCN NNGCCACGTT GTGAGTTNNG ATAGTTGTGG AAAGAGTCNA NTGGCTCTCN
              601 NCAAGCGTAN TCNNNNAGGG GCTGAAGGNN NNCCNNNNNN NNCCCNTTGT NNGGGATCTG
              661 ATNNGGNNCC TNNNNGNNNN NGNNTNACNN GTGTNANNNN NNNNNAAAAA ANNTCTANGN
            721 CNNCNNNNNN NNNN
            //
            Primer: Reverse-2
            Region: bGHpA,PuroR
            Sequence file: RDB07100_A5Ede.seq check
            >07100_07100_A5Ed_Reverse2_E06_15.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNTNGGCTGC AGGTTCTTTC CGCCTCNGAA GCCATAGAGC
               61 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC
              121 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT
              181 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG
              241 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGAGCTCTAG
              301 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
              361 CGTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG
              421 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC
              481 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG
              541 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG
              601 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG
              661 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG
              721 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG
              781 ATGTCGAGCC CGANGCGCGT GANGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG
              841 CGGTCCGGAT CGACGGTGTG GCGCGTGGCG GGGTANTCGG CGAACGCGGC GGCNANGGNG
              901 CGTACNGNCC NNGGGNNNNC GNCNCGGGNG GCGAGGCGCA CCNNGGGCTT GNACTCGGNC
              961 ANNGNNNGNG GNCNATNTTA TCNTCGNGNT TTNCNANNNA AAANNNNNTC CCNNNNGNNN
            1021 NNGGGGGNNN NNNNNNN
            //
            Primer: Amp_R
            Region: Polyoma ori,SV40 pA,CMV en
            Sequence file: RDB07100_A5Edf.seq check
            >07100_07100_A9DnF_Amp_R_C10_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGCTT TNGNNNNNNA GANTGTATTT CCTGGAAATT AATGTTTATT
               61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
              121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
              181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
              241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
              301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
              361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
              421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
              481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT
              541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA
              601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
              661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC
              721 TGATATCATC GTCGACATTG ATTATTGACT AGCTAGTTAT TAATAGTAAT CAATTACGGG
              781 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACNG TAAATGGNCC
              841 GCCTGGCTGA NCGCCCNACG ACCCCCGCCC ATTGACGTCN ATAATGACGT ATGTTCCNNT
              901 AGTNACGNCN ATAGGGACTT TCNNTTGNCG TCANTGGGNG GANTATTTAN NNNNAACTGN
              961 CNACTTNGCA NTACATNNNG NGTATNNNNT GNCNNNNTAN GCCCCCNNNT NNNGNNNNNN
            1021 NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818 .

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl