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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-cHA-IP (#RDB07100)

Expression vector with CAG promoter, HA-tag, Puromycin selection.

Alternative name pCAGIP, pPyCAG-cHA-IP, stock no 1426
Clone info. Expression vector with CAG promoter, HA-tag, SV40 poly(A) and polyoma ori. Puromycin selection.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells)
Gene/insert name cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence (full) RDB07100zzk02.seq provided by the depositor
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 22, 1526-1536 (2002))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07100 pPyCAG-cHA-IP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-cHA-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07100).

Reference section:

Niwa, H., Masui, S., Chambers, I., Smith, A.G., Miyazaki, J., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07100_A5Edp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: CAG pro,HA,IRES 5'
Sequence file: RDB07100_A5Edd.seq check
>07100_07100_B1BjS_pAxCA_F1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC CTTCTTCTTT TTCCTACNGC TCCTGGGCAA CGTGCTGGTT
   61 ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGA TCTCACGCGT GACTAGTGCC
  121 ATGGCCTACC CATACGACGT GCCAGACTAC GCCTAGCGGC CGCTCGATAA GCTTGATATC
  181 GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
  241 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
  301 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
  361 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
  421 AGCTTCTNGN NNACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
  481 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGNATAA NATACACCTG CAAAGGCGGC
  541 ACNNNCCNCN NNGCCACGTT GTGAGTTNNG ATAGTTGTGG AAAGAGTCNA NTGGCTCTCN
  601 NCAAGCGTAN TCNNNNAGGG GCTGAAGGNN NNCCNNNNNN NNCCCNTTGT NNGGGATCTG
  661 ATNNGGNNCC TNNNNGNNNN NGNNTNACNN GTGTNANNNN NNNNNAAAAA ANNTCTANGN
721 CNNCNNNNNN NNNN
//
Primer: Reverse-2 (Pr0100)
Region: bGHpA,PuroR
Sequence file: RDB07100_A5Ede.seq check
>07100_07100_A5Ed_Reverse2_E06_15.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTNGGCTGC AGGTTCTTTC CGCCTCNGAA GCCATAGAGC
   61 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC
  121 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT
  181 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG
  241 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGAGCTCTAG
  301 ATCATCGATG CATGGGGTCG TGCGCTCCTT TCGGTCGGGC GCTGCGGGTC GTGGGGCGGG
  361 CGTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG
  421 TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC
  481 TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG
  541 CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG
  601 GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG
  661 GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG
  721 CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG
  781 ATGTCGAGCC CGANGCGCGT GANGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG
  841 CGGTCCGGAT CGACGGTGTG GCGCGTGGCG GGGTANTCGG CGAACGCGGC GGCNANGGNG
  901 CGTACNGNCC NNGGGNNNNC GNCNCGGGNG GCGAGGCGCA CCNNGGGCTT GNACTCGGNC
  961 ANNGNNNGNG GNCNATNTTA TCNTCGNGNT TTNCNANNNA AAANNNNNTC CCNNNNGNNN
1021 NNGGGGGNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: Polyoma ori,SV40 pA,CMV en
Sequence file: RDB07100_A5Edf.seq check
>07100_07100_A9DnF_Amp_R_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCTT TNGNNNNNNA GANTGTATTT CCTGGAAATT AATGTTTATT
   61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
  121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
  181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
  241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
  301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
  361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
  421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
  481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT
  541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA
  601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC
  721 TGATATCATC GTCGACATTG ATTATTGACT AGCTAGTTAT TAATAGTAAT CAATTACGGG
  781 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACNG TAAATGGNCC
  841 GCCTGGCTGA NCGCCCNACG ACCCCCGCCC ATTGACGTCN ATAATGACGT ATGTTCCNNT
  901 AGTNACGNCN ATAGGGACTT TCNNTTGNCG TCANTGGGNG GANTATTTAN NNNNAACTGN
  961 CNACTTNGCA NTACATNNNG NGTATNNNNT GNCNNNNTAN GCCCCCNNNT NNNGNNNNNN
1021 NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818. [link to RRC of NBRP]