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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-BstXI-IP (#RDB07099)

Mammalian expression vector. Blasticidin resistance.

Alternative name pCAGIP, pCAGIPuro, pPyCAG-BstXI-IP, stock no 544
Clone info. Mammalian expression vector. Puromycin resistance.
Comment SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone Plasmid
Gene/insert name cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB07099z.seq
Additional map

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Cell. Biol., 22, 1526-1536 (2002))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07099 pPyCAG-BstXI-IP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-BstXI-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07099).

Reference section:

Niwa, H., Masui, S., Chambers, I., Smith, A.G., Miyazaki, J., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07099_A5Eep1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAGen_R1 ()
Region: HCMVIEE,SV40p(A),Polyoma ori
Sequence file: RDB07099_A5Eec.seq check
>07099_07099_A9I3F_CAG_R1_A10_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCGGGCGC GGGGCGAANC GGTGCGGCGC CGGCNGGAAG
   61 GAAATGGGCG GGGANGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG
  121 CCTCGGGGCT GTCCGCGGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC
  181 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC
  241 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA
  301 GAATTCCTCG AGATCCATTG TGCTGGCGGT AGGCGAGCAG CGCCTGCCTG AAGCTGCGGG
  361 CATTCCCGAT CAGAAATGAG CGCCAGTCGT CGTCGGCTCT CGGCACCGAA TGCGTATGAT
  421 TCTCCGCCAG CATGGCTTCG GCCAGTGCGT CGAGCAGCGC CCGCTTGTTC CTGAAGTGCC
  481 AGTAAAGCGC CGGCTGCTGA ACCCCCAACC GTTCCGCCAG TTTGCGTGTC GTCAGACCGT
  541 CTACGCCGAC CTCGTTCAAC AGGTCCAGGG CGGCACGGAT CACTGTATTC GGCTGCAACT
  601 TTGTCATGCT TGACACTTTA TCACTGATAA ACATAATATG TCCACCAACT TATCAGTGAT
  661 AAAGAATCCG CGCCAGCACA ATGGATCTCG AGGAATTCAC TCCTCAGGTG CAGGCTGCCT
  721 ATCAGANNGT GGTGGCTGGT GTGGCCAACT GGCTCACAAA TACCACTGAG ATCGACGGTA
  781 TCGAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TNCCTCCCCC
  841 CCCCCTAACG TTACNNGCCG AAGCNGCTNN NAATAANGNC GGNGTGCNNT TGTCTATATG
  901 TTATTTTCCA CCNTATNGCC NNCTTTTGGN ANNNTNNNGG NCNNGNNAAC CTGNNCNNNN
961 NNNNNN
//
Primer: BGH_rev2 ()
Region: bGHp(A),PuroR,IRES
Sequence file: RDB07099_A5Eed.seq check
>07099_07099_A9I3F_IRES-M_R_B10_02_ABI08.ab1
    1 NNNNNNNNNN NNGCTTCNTT NNNGACNTTN NACAGACCTT GCATTCCTTT GGCGAGAGGG
   61 GAAAGACCCC TAGGAATGCT CGTCAAGAAG ACAGGGCCAG GTTTCCGGGC CCTCACATTG
  121 CCAAAAGACG GCAATATGGT GGAAAATAAC ATATAGACAA ACGCACACCG GCCTTATTCC
  181 AAGCGGCTTC GGCCAGTAAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC
  241 GATATCAAGC TTATCGAGCG GCCGCTCGAT ACCGTCGATC TCAGTGGTAT TTGTGANNCN
  301 NNTGGCCACA CCAGCCACCA CCTTCTGATA GGCANCCTGC NCCTGANGAN TGAATTNNNN
  361 NNGATNNNNN NNGCTGGNNC GGATTCNTTA TCACTGATNA NTTGGTGGAC NTNNNNNGTT
421 TATCAGTGNN NNNNNNNN
//
Primer: CAGen_R1
Sequence file: RDB07099_A5Eee.seq check
>07099_07099_A9I3F_CAGen_R1_C10_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNG GCGGTCNGCC NGGCGGGCCA TTTACCGTAA
   61 GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT
  121 ATTAATAACT AGTCAATAAT CAATGTCGAC GATGATATCA GATCCAGACA TGATAAGATA
  181 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA
  241 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAATTC
  301 GATTCAGCTT CAGAAGATGG CGGAGGGCCT CCAACACAGT AATTTTCCTC CCGACTCTTA
  361 AAATAGAAAA TGTCAAGTCA GTTAAGCAGG AAGTGACTAA CTGACCGCAG CTGGCCGTGC
  421 GACATCCTCT TTTAATTAGT TGCTAGGCAA CTGCCCTCCA GAGGGCAGTG TGGTTTTGCA
  481 AGAGGAAGCA AAAAGCCTCT CCACCCAGGC CTGGAATGTC AGTGTGGTTT TGCAAGAGGA
  541 AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTTTCCACC CAATCATTAC TATGACAACA
  601 GCTGTTTTTT TTAGTATTAA GCAGAGGCCG GGGGCCCCTG GCCTCCGCTT ACTCTGGANA
  661 AAAAGAAGAG AGGCTTCCAG AGGCAACTTG TCAAAACAGG ACTGGCGCCT TGGANGCGCT
  721 GTGGGGCCAC CCAAATTGAT ATAATTAAGC CCCAACCGCC TCTTCCCGCC TCATTTCAGC
  781 CTCACCACTA TGTGAATATA GCTGAATACA CAGTTTATTG AATAAACATT AATTTCCNGG
  841 AAATACAGTC TTTGTTTTTC CNAAGCGGTC ATTGNAAAAA GGAAGANTAT GANTANTCAA
  901 CATTNNCGNN TCGCCCTTAT TCCCTTTTTT TGCGGCATTT TGCCNNCCTG TTTTTTGCNN
961 ACCCANNAAC NCNNGNGAAN GTAAANNNNG NNNNANNNNN NNNNGNNNNN NNNNN
//
Primer: BGH_rev2
Sequence file: RDB07099_A5Eef.seq check
>07099_07099_A9I3F_BGH_rev2_D10_04_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC
  121 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG
  181 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG
  241 GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC
  301 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG
  361 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC
  421 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG
  481 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG
  541 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC
  601 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG
  661 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG
  721 CGCACCGTGG GCTTGTACTC GGTCATGGTT GTGGCCATAT TATCATCGTG TTTTTCAAAG
  781 GAAAACCACG TCCCCNNGGT TCGGGGGGCC TAGACGTTTT TTTAACCTCN ACTAAACACA
  841 TGTAAAGCAT GTGCACCGAN GCCCCAGATC AGATNCNNTA CNANGGGGGT ANCNTCNGGG
  901 CATCCTTCAN CCCCTNGTTG AANNCGCTNG ANGNNANCNN NTTNGANTNN TTCCNCNNNN
961 NTCNNANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818. [link to RRC of NBRP]
reference Hung, S.S., Repression of global protein synthesis by Eif1a-like genes that are expressed specifically in the two-cell embryos and the transient Zscan4-positive state of embryonic stem cells. DNA Res. 2013 Aug;20(4):391-402 (2013). PMID 23649898. [link to RRC of NBRP]
reference Hirata, T., Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells. Sci. Rep. 2: 208 (2012).. PMID 22355722. [link to RRC of NBRP]