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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-BstXI-IP

Mammalian expression vector. Blasticidin resistance.

Catalog number RDB07099
Resource name pPyCAG-BstXI-IP
Alternative name pCAGIP, pCAGIPuro, pPyCAG-BstXI-IP, stock no 544
Clone info. Mammalian expression vector. Puromycin resistance.
Comment SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone Plasmid
Gene/insert name cDNA
Depositor|Developer Niwa, Hitoshi |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Cell. Biol., 22, 1526-1536 (2002))。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB07099 pPyCAG-BstXI-IP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB07099z.seq
            Remarks, protocol and/or map (gif) RDB07099a.gif

            Original reference

            original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07099_A5Eep1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CAGen_R1
            Region: HCMVIEE,SV40p(A),Polyoma ori
            Sequence file: RDB07099_A5Eec.seq check
            >07099_07099_A9I3F_CAG_R1_A10_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGCGGGCGC GGGGCGAANC GGTGCGGCGC CGGCNGGAAG
               61 GAAATGGGCG GGGANGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG
              121 CCTCGGGGCT GTCCGCGGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC
              181 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC
              241 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA
              301 GAATTCCTCG AGATCCATTG TGCTGGCGGT AGGCGAGCAG CGCCTGCCTG AAGCTGCGGG
              361 CATTCCCGAT CAGAAATGAG CGCCAGTCGT CGTCGGCTCT CGGCACCGAA TGCGTATGAT
              421 TCTCCGCCAG CATGGCTTCG GCCAGTGCGT CGAGCAGCGC CCGCTTGTTC CTGAAGTGCC
              481 AGTAAAGCGC CGGCTGCTGA ACCCCCAACC GTTCCGCCAG TTTGCGTGTC GTCAGACCGT
              541 CTACGCCGAC CTCGTTCAAC AGGTCCAGGG CGGCACGGAT CACTGTATTC GGCTGCAACT
              601 TTGTCATGCT TGACACTTTA TCACTGATAA ACATAATATG TCCACCAACT TATCAGTGAT
              661 AAAGAATCCG CGCCAGCACA ATGGATCTCG AGGAATTCAC TCCTCAGGTG CAGGCTGCCT
              721 ATCAGANNGT GGTGGCTGGT GTGGCCAACT GGCTCACAAA TACCACTGAG ATCGACGGTA
              781 TCGAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TNCCTCCCCC
              841 CCCCCTAACG TTACNNGCCG AAGCNGCTNN NAATAANGNC GGNGTGCNNT TGTCTATATG
              901 TTATTTTCCA CCNTATNGCC NNCTTTTGGN ANNNTNNNGG NCNNGNNAAC CTGNNCNNNN
            961 NNNNNN
            //
            Primer: BGH_rev2
            Region: bGHp(A),PuroR,IRES
            Sequence file: RDB07099_A5Eed.seq check
            >07099_07099_A9I3F_IRES-M_R_B10_02_ABI08.ab1
                1 NNNNNNNNNN NNGCTTCNTT NNNGACNTTN NACAGACCTT GCATTCCTTT GGCGAGAGGG
               61 GAAAGACCCC TAGGAATGCT CGTCAAGAAG ACAGGGCCAG GTTTCCGGGC CCTCACATTG
              121 CCAAAAGACG GCAATATGGT GGAAAATAAC ATATAGACAA ACGCACACCG GCCTTATTCC
              181 AAGCGGCTTC GGCCAGTAAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC
              241 GATATCAAGC TTATCGAGCG GCCGCTCGAT ACCGTCGATC TCAGTGGTAT TTGTGANNCN
              301 NNTGGCCACA CCAGCCACCA CCTTCTGATA GGCANCCTGC NCCTGANGAN TGAATTNNNN
              361 NNGATNNNNN NNGCTGGNNC GGATTCNTTA TCACTGATNA NTTGGTGGAC NTNNNNNGTT
            421 TATCAGTGNN NNNNNNNN
            //
            Primer: CAGen_R1
            Sequence file: RDB07099_A5Eee.seq check
            >07099_07099_A9I3F_CAGen_R1_C10_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNG GCGGTCNGCC NGGCGGGCCA TTTACCGTAA
               61 GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT
              121 ATTAATAACT AGTCAATAAT CAATGTCGAC GATGATATCA GATCCAGACA TGATAAGATA
              181 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA
              241 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAATTC
              301 GATTCAGCTT CAGAAGATGG CGGAGGGCCT CCAACACAGT AATTTTCCTC CCGACTCTTA
              361 AAATAGAAAA TGTCAAGTCA GTTAAGCAGG AAGTGACTAA CTGACCGCAG CTGGCCGTGC
              421 GACATCCTCT TTTAATTAGT TGCTAGGCAA CTGCCCTCCA GAGGGCAGTG TGGTTTTGCA
              481 AGAGGAAGCA AAAAGCCTCT CCACCCAGGC CTGGAATGTC AGTGTGGTTT TGCAAGAGGA
              541 AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTTTCCACC CAATCATTAC TATGACAACA
              601 GCTGTTTTTT TTAGTATTAA GCAGAGGCCG GGGGCCCCTG GCCTCCGCTT ACTCTGGANA
              661 AAAAGAAGAG AGGCTTCCAG AGGCAACTTG TCAAAACAGG ACTGGCGCCT TGGANGCGCT
              721 GTGGGGCCAC CCAAATTGAT ATAATTAAGC CCCAACCGCC TCTTCCCGCC TCATTTCAGC
              781 CTCACCACTA TGTGAATATA GCTGAATACA CAGTTTATTG AATAAACATT AATTTCCNGG
              841 AAATACAGTC TTTGTTTTTC CNAAGCGGTC ATTGNAAAAA GGAAGANTAT GANTANTCAA
              901 CATTNNCGNN TCGCCCTTAT TCCCTTTTTT TGCGGCATTT TGCCNNCCTG TTTTTTGCNN
            961 ACCCANNAAC NCNNGNGAAN GTAAANNNNG NNNNANNNNN NNNNGNNNNN NNNNN
            //
            Primer: BGH_rev2
            Sequence file: RDB07099_A5Eef.seq check
            >07099_07099_A9I3F_BGH_rev2_D10_04_ABI08.ab1
                1 NNNNNNNNNN NNNGNNNNNN NNNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
               61 TCGAGGCTGA TCAGCGAGCT CTAGATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC
              121 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG
              181 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG
              241 GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC
              301 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG
              361 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC
              421 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG
              481 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG
              541 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC
              601 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG
              661 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG
              721 CGCACCGTGG GCTTGTACTC GGTCATGGTT GTGGCCATAT TATCATCGTG TTTTTCAAAG
              781 GAAAACCACG TCCCCNNGGT TCGGGGGGCC TAGACGTTTT TTTAACCTCN ACTAAACACA
              841 TGTAAAGCAT GTGCACCGAN GCCCCAGATC AGATNCNNTA CNANGGGGGT ANCNTCNGGG
              901 CATCCTTCAN CCCCTNGTTG AANNCGCTNG ANGNNANCNN NTTNGANTNN TTCCNCNNNN
            961 NTCNNANNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.
            reference Hung, S.S., Repression of global protein synthesis by Eif1a-like genes that are expressed specifically in the two-cell embryos and the transient Zscan4-positive state of embryonic stem cells. DNA Res. 2013 Aug;20(4):391-402 (2013). PMID 23649898.
            reference Hirata, T., Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells. Sci. Rep. 2: 208 (2012).. PMID 22355722.

            2022.05.17

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