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pPyCAG-BstXI-IP

Mammalian expression vector. Blasticidin resistance.

Catalog number RDB07099
Resource name pPyCAG-BstXI-IP
Alternative name pCAGIP, pCAGIPuro, pPyCAG-BstXI-IP, stock no 544
Clone info. Mammalian expression vector. Puromycin resistance.
SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone Plasmid
Gene/insert name cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07099 pPyCAG-BstXI-IP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07099z.seq from Depositor
Electronic file Map RDB07099a.gif from Depositor

Original reference

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07099_A5Eep1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: HCMVIEE,SV40p(A),Polyoma ori | Primer: CAGen_R1 | Sequence: RDB07099_A5Eec.seq
>07099_07099_A9I3F_CAG_R1_A10_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCGGGCGC GGGGCGAANC GGTGCGGCGC CGGCNGGAAG
   61 GAAATGGGCG GGGANGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG
  121 CCTCGGGGCT GTCCGCGGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC
  181 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC
  241 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA
  301 GAATTCCTCG AGATCCATTG TGCTGGCGGT AGGCGAGCAG CGCCTGCCTG AAGCTGCGGG
  361 CATTCCCGAT CAGAAATGAG CGCCAGTCGT CGTCGGCTCT CGGCACCGAA TGCGTATGAT
  421 TCTCCGCCAG CATGGCTTCG GCCAGTGCGT CGAGCAGCGC CCGCTTGTTC CTGAAGTGCC
  481 AGTAAAGCGC CGGCTGCTGA ACCCCCAACC GTTCCGCCAG TTTGCGTGTC GTCAGACCGT
  541 CTACGCCGAC CTCGTTCAAC AGGTCCAGGG CGGCACGGAT CACTGTATTC GGCTGCAACT
  601 TTGTCATGCT TGACACTTTA TCACTGATAA ACATAATATG TCCACCAACT TATCAGTGAT
  661 AAAGAATCCG CGCCAGCACA ATGGATCTCG AGGAATTCAC TCCTCAGGTG CAGGCTGCCT
  721 ATCAGANNGT GGTGGCTGGT GTGGCCAACT GGCTCACAAA TACCACTGAG ATCGACGGTA
  781 TCGAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TNCCTCCCCC
  841 CCCCCTAACG TTACNNGCCG AAGCNGCTNN NAATAANGNC GGNGTGCNNT TGTCTATATG
  901 TTATTTTCCA CCNTATNGCC NNCTTTTGGN ANNNTNNNGG NCNNGNNAAC CTGNNCNNNN
  961 NNNNNNNNNN NNNNNTNNNN NGGNNNNTNN NNNNNNNNNN NANNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNN
//
Region: bGHp(A),PuroR,IRES | Primer: BGH_rev2 | Sequence: RDB07099_A5Eed.seq
>07099_07099_A9I3F_IRES-M_R_B10_02_ABI08.ab1
    1 NNNNNNNNNN NNGCTTCNTT NNNGACNTTN NACAGACCTT GCATTCCTTT GGCGAGAGGG
   61 GAAAGACCCC TAGGAATGCT CGTCAAGAAG ACAGGGCCAG GTTTCCGGGC CCTCACATTG
  121 CCAAAAGACG GCAATATGGT GGAAAATAAC ATATAGACAA ACGCACACCG GCCTTATTCC
  181 AAGCGGCTTC GGCCAGTAAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC
  241 GATATCAAGC TTATCGAGCG GCCGCTCGAT ACCGTCGATC TCAGTGGTAT TTGTGANNCN
  301 NNTGGCCACA CCAGCCACCA CCTTCTGATA GGCANCCTGC NCCTGANGAN TGAATTNNNN
  361 NNGATNNNNN NNGCTGGNNC GGATTCNTTA TCACTGATNA NTTGGTGGAC NTNNNNNGTT
  421 TATCAGTGNN NNNNNNNNAA NNATGANNNA NTTGCAGCCG ANNNCAGTGA TCCGTGCCGC
  481 CCTGNNNNNN NNGAACNAGG TCNNNNNNNN NNTCTGACNA CACGCNNNCT GNNNNANNNN
  541 NNGGNNGNTN NNNNNNNNNN GCNNNNNNGN NACTNNNNGA NNNAGCGGGC GCTGNTNNNN
  601 NNANTGGNNN NNNNCNTGCT GNNNGAGAAT CNTNNNNNTT CNNNGNNNNN NNNGACGANN
  661 ACTGNNNNNN NTTTNTGNNN NNNNNNNNNC GCNNNNNNNN GNNNNNNNNN NNNNNNNNNC
  721 NNCNNNNNNN NGNNNNNCNN NNNNTTNNTN NNNNNNNNNA NNNNNANNNN NNNNNNNNNN
  781 NNNNNNNNNN CANNNNNTGN TNNNNNNAAN NNNNAANGCA TGNNNCNTGG GTTNNNNNNN
  841 NNNNCNNNAN NNNNNNNNNN NNNNNNNNNN NAAAGCCNAN NNNCNNNNNC NNNNNNNCNN
  901 NNNNNNCNNN NNNAGGNNGN NNNNNNCCCC CNNNNNNNNN NNNNNCCNNA NNNNNNNNNN
  961 NNNNANAANN NNNAANNNCN NNNNNNNNNN NCNNNNNNNN ANNGCNCNNN NNNNNNNNCC
1021 NNNTTNNNNN NNTNNNNNNN NNNNNNNNNN NNNNNNNNNN NNN
//
Primer: CAGen_R1 | Sequence: RDB07099_A5Eee.seq
>07099_07099_A9I3F_CAGen_R1_C10_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNG GCGGTCNGCC NGGCGGGCCA TTTACCGTAA
   61 GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT
  121 ATTAATAACT AGTCAATAAT CAATGTCGAC GATGATATCA GATCCAGACA TGATAAGATA
  181 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA
  241 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAATTC
  301 GATTCAGCTT CAGAAGATGG CGGAGGGCCT CCAACACAGT AATTTTCCTC CCGACTCTTA
  361 AAATAGAAAA TGTCAAGTCA GTTAAGCAGG AAGTGACTAA CTGACCGCAG CTGGCCGTGC
  421 GACATCCTCT TTTAATTAGT TGCTAGGCAA CTGCCCTCCA GAGGGCAGTG TGGTTTTGCA
  481 AGAGGAAGCA AAAAGCCTCT CCACCCAGGC CTGGAATGTC AGTGTGGTTT TGCAAGAGGA
  541 AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTTTCCACC CAATCATTAC TATGACAACA
  601 GCTGTTTTTT TTAGTATTAA GCAGAGGCCG GGGGCCCCTG GCCTCCGCTT ACTCTGGANA
  661 AAAAGAAGAG AGGCTTCCAG AGGCAACTTG TCAAAACAGG ACTGGCGCCT TGGANGCGCT
  721 GTGGGGCCAC CCAAATTGAT ATAATTAAGC CCCAACCGCC TCTTCCCGCC TCATTTCAGC
  781 CTCACCACTA TGTGAATATA GCTGAATACA CAGTTTATTG AATAAACATT AATTTCCNGG
  841 AAATACAGTC TTTGTTTTTC CNAAGCGGTC ATTGNAAAAA GGAAGANTAT GANTANTCAA
  901 CATTNNCGNN TCGCCCTTAT TCCCTTTTTT TGCGGCATTT TGCCNNCCTG TTTTTTGCNN
  961 ACCCANNAAC NCNNGNGAAN GTAAANNNNG NNNNANNNNN NNNNGNNNNN NNNNNNGGNN
 1021 TNNNNNNNNA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNN
//
Primer: BGH_rev2 | Sequence: RDB07099_A5Eef.seq
>07099_07099_A9I3F_BGH_rev2_D10_04_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC
  121 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG
  181 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG
  241 GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC
  301 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG
  361 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC
  421 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG
  481 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG
  541 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC
  601 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG
  661 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG
  721 CGCACCGTGG GCTTGTACTC GGTCATGGTT GTGGCCATAT TATCATCGTG TTTTTCAAAG
  781 GAAAACCACG TCCCCNNGGT TCGGGGGGCC TAGACGTTTT TTTAACCTCN ACTAAACACA
  841 TGTAAAGCAT GTGCACCGAN GCCCCAGATC AGATNCNNTA CNANGGGGGT ANCNTCNGGG
  901 CATCCTTCAN CCCCTNGTTG AANNCGCTNG ANGNNANCNN NTTNGANTNN TTCCNCNNNN
  961 NTCNNANNNN NNNNNNNNNN NNNNGNGNTN NNNNCNNNCN NTNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.
reference Hung, S.S., Repression of global protein synthesis by Eif1a-like genes that are expressed specifically in the two-cell embryos and the transient Zscan4-positive state of embryonic stem cells. DNA Res. 2013 Aug;20(4):391-402 (2013). PMID 23649898.
reference Hirata, T., Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells. Sci. Rep. 2: 208 (2012).. PMID 22355722.

2019.11.02

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