Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-BstXI-IP

Mammalian expression vector. Blasticidin resistance.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Cell. Biol., 22, 1526-1536 (2002))。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB07099z.seq
Remarks, protocol and/or map (gif)	RDB07099a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07099_A5Eep1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAGen_R1 Region: HCMVIEE,SV40p(A),Polyoma ori Sequence file: RDB07099_A5Eec.seq
>07099_07099_A9I3F_CAG_R1_A10_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCGGGCGC GGGGCGAANC GGTGCGGCGC CGGCNGGAAG    61 GAAATGGGCG GGGANGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG   121 CCTCGGGGCT GTCCGCGGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC   181 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC   241 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA   301 GAATTCCTCG AGATCCATTG TGCTGGCGGT AGGCGAGCAG CGCCTGCCTG AAGCTGCGGG   361 CATTCCCGAT CAGAAATGAG CGCCAGTCGT CGTCGGCTCT CGGCACCGAA TGCGTATGAT   421 TCTCCGCCAG CATGGCTTCG GCCAGTGCGT CGAGCAGCGC CCGCTTGTTC CTGAAGTGCC   481 AGTAAAGCGC CGGCTGCTGA ACCCCCAACC GTTCCGCCAG TTTGCGTGTC GTCAGACCGT   541 CTACGCCGAC CTCGTTCAAC AGGTCCAGGG CGGCACGGAT CACTGTATTC GGCTGCAACT   601 TTGTCATGCT TGACACTTTA TCACTGATAA ACATAATATG TCCACCAACT TATCAGTGAT   661 AAAGAATCCG CGCCAGCACA ATGGATCTCG AGGAATTCAC TCCTCAGGTG CAGGCTGCCT   721 ATCAGANNGT GGTGGCTGGT GTGGCCAACT GGCTCACAAA TACCACTGAG ATCGACGGTA   781 TCGAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TNCCTCCCCC   841 CCCCCTAACG TTACNNGCCG AAGCNGCTNN NAATAANGNC GGNGTGCNNT TGTCTATATG   901 TTATTTTCCA CCNTATNGCC NNCTTTTGGN ANNNTNNNGG NCNNGNNAAC CTGNNCNNNN 961 NNNNNN //
Primer: BGH_rev2 Region: bGHp(A),PuroR,IRES Sequence file: RDB07099_A5Eed.seq
>07099_07099_A9I3F_IRES-M_R_B10_02_ABI08.ab1     1 NNNNNNNNNN NNGCTTCNTT NNNGACNTTN NACAGACCTT GCATTCCTTT GGCGAGAGGG    61 GAAAGACCCC TAGGAATGCT CGTCAAGAAG ACAGGGCCAG GTTTCCGGGC CCTCACATTG   121 CCAAAAGACG GCAATATGGT GGAAAATAAC ATATAGACAA ACGCACACCG GCCTTATTCC   181 AAGCGGCTTC GGCCAGTAAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC   241 GATATCAAGC TTATCGAGCG GCCGCTCGAT ACCGTCGATC TCAGTGGTAT TTGTGANNCN   301 NNTGGCCACA CCAGCCACCA CCTTCTGATA GGCANCCTGC NCCTGANGAN TGAATTNNNN   361 NNGATNNNNN NNGCTGGNNC GGATTCNTTA TCACTGATNA NTTGGTGGAC NTNNNNNGTT 421 TATCAGTGNN NNNNNNNN //
Primer: CAGen_R1 Sequence file: RDB07099_A5Eee.seq
>07099_07099_A9I3F_CAGen_R1_C10_03_ABI08.ab1     1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNG GCGGTCNGCC NGGCGGGCCA TTTACCGTAA    61 GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT   121 ATTAATAACT AGTCAATAAT CAATGTCGAC GATGATATCA GATCCAGACA TGATAAGATA   181 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA   241 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAATTC   301 GATTCAGCTT CAGAAGATGG CGGAGGGCCT CCAACACAGT AATTTTCCTC CCGACTCTTA   361 AAATAGAAAA TGTCAAGTCA GTTAAGCAGG AAGTGACTAA CTGACCGCAG CTGGCCGTGC   421 GACATCCTCT TTTAATTAGT TGCTAGGCAA CTGCCCTCCA GAGGGCAGTG TGGTTTTGCA   481 AGAGGAAGCA AAAAGCCTCT CCACCCAGGC CTGGAATGTC AGTGTGGTTT TGCAAGAGGA   541 AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTTTCCACC CAATCATTAC TATGACAACA   601 GCTGTTTTTT TTAGTATTAA GCAGAGGCCG GGGGCCCCTG GCCTCCGCTT ACTCTGGANA   661 AAAAGAAGAG AGGCTTCCAG AGGCAACTTG TCAAAACAGG ACTGGCGCCT TGGANGCGCT   721 GTGGGGCCAC CCAAATTGAT ATAATTAAGC CCCAACCGCC TCTTCCCGCC TCATTTCAGC   781 CTCACCACTA TGTGAATATA GCTGAATACA CAGTTTATTG AATAAACATT AATTTCCNGG   841 AAATACAGTC TTTGTTTTTC CNAAGCGGTC ATTGNAAAAA GGAAGANTAT GANTANTCAA   901 CATTNNCGNN TCGCCCTTAT TCCCTTTTTT TGCGGCATTT TGCCNNCCTG TTTTTTGCNN 961 ACCCANNAAC NCNNGNGAAN GTAAANNNNG NNNNANNNNN NNNNGNNNNN NNNNN //
Primer: BGH_rev2 Sequence file: RDB07099_A5Eef.seq
>07099_07099_A9I3F_BGH_rev2_D10_04_ABI08.ab1     1 NNNNNNNNNN NNNGNNNNNN NNNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGAGCT CTAGATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC   121 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG   181 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG   241 GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC   301 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG   361 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC   421 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG   481 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG   541 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC   601 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG   661 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG   721 CGCACCGTGG GCTTGTACTC GGTCATGGTT GTGGCCATAT TATCATCGTG TTTTTCAAAG   781 GAAAACCACG TCCCCNNGGT TCGGGGGGCC TAGACGTTTT TTTAACCTCN ACTAAACACA   841 TGTAAAGCAT GTGCACCGAN GCCCCAGATC AGATNCNNTA CNANGGGGGT ANCNTCNGGG   901 CATCCTTCAN CCCCTNGTTG AANNCGCTNG ANGNNANCNN NTTNGANTNN TTCCNCNNNN 961 NTCNNANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl