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pPyCAG-BstXI-IP

Mammalian expression vector. Blasticidin resistance.

Catalog number RDB07099
Resource name pPyCAG-BstXI-IP
Alternative name pCAGIP, pCAGIPuro, pPyCAG-BstXI-IP, stock no 544
Clone info. Mammalian expression vector. Puromycin resistance.
SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone Plasmid
Gene/insert name cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 22, 1526-1536 (2002))
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07099 pPyCAG-BstXI-IP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07099z.seq from Depositor
Electronic file Map RDB07099a.gif from Depositor

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References

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07099_A5Ee.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB07099_A5Eea.seq
>07099_07099_A5Ee_pAxCA_F1_A03_03.ab1
    1 CTCAGAAGCT GGAGGGTCTG GCCACTTGGT TATGCCTTCT TCGTTTTGTC CTGCAGCGTC
   61 CCTGGGGAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCCTCGAGAA
  121 TCCATTGTGC TGGCGGTAGG CGAGCAGCGC CTGCCTGAAG CTGCGGGCAT TCCCGATCAG
  181 AAATGAGCGC CAGTCGTCGT CGGCTCTCGG CACCGAATGC GTATGATTCT CCGCCAGCAT
  241 GGCTTCGGCC AGTGCGTCGA GCAGCGCCCG CTTGTTCCTG AAGTGCCAGT AAAGCGCCGG
  301 CTGCTGAACC CCCAACCGTT CCGCCAGTTT GCGTGTCGTC AGACCGTCTA CGCCGACCTC
  361 GTTCAACAGG TCCAGGGCGG CACGGATCAC TGTATTCGGC TGCAACTTTG TCATGCTTGA
  421 CACTTTATCA CTGATAAACA TAATATGTCC ACCAACTTAT CAGTGATAAA GAATCCGCGC
  481 CAGCACAATG GATCTCGAGG AATTCACTCC TCAGGTGCAG GCTGCCTATC AGAAGGTGGT
  541 GGCTGGTGTG GCCAACTGGC TCACAAATAC CACTGAGATC GACGGTATCG AGCGGCCGCT
  601 CGATAAGCTT GATATCGAAT TCCGCCCCCC CCCCCTCTCC CTCCCCCCCC CCTAACGTTA
  661 CTGGCCGAAG CCGCTTGGAA ATAAGGCCGG TGTGGCGTTT TGTCTATATG TTTATTTTCC
721 CCC
//
Primer: Reverse2 RDB07099_A5Eeb.seq
>07099_07099_A5Ee_Reverse2_B03_06.ab1
    1 AAGTAACAGG GAAATTCGCC CAAGCCTTGG GGCTGCAGGT TCTTTCCGCC TCAGAAGCCA
   61 TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT
  121 CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT
  181 CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG
  241 GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG
  301 CTCTAGATCA TCGATGCATG GGGTCGTGCG CTCCTTTCGG TCGGGCGCTG CGGGTCGTGG
  361 GGCGGGCGTC AGGCACCGGG CTTGCGGGTC ATGCACCAGG TGCGCGGTCC TTCGGGCACC
  421 TCGACGTCGG CGGTGACGGT GAAGCCGAGC CGCTCGTAGA AGGGGAGGTT GCGGGGCGCG
  481 GAGGTCTCCA GGAAGGCGGG CACCCCGGCG CGCTCGGCCG CCTCCACTCC GGGGAGCACG
  541 ACGGCGCTGC CCAGACCCTT GCCCTGGTGG TCGGGCGAGA CGCCGACGGT GGCCAGGAAC
  601 CACGCGGGCT CCTTGGGCCG GTGCGGCGCC AGGAGGCCTT CCATCTGTTG CTGCGCGGCC
  661 AGCCGGGAAC CGCTCAACTC GGCCATGCGC GGGCCGATCT CGGCGAACAC CGCCCCCGCT
  721 TCGACGCTCT CCGGCGTGGT CCAGACCGCC ACCGCGGCGC CGTCGTCCGC GACCCACACC
  781 TTGCCGATGT CGAGCCCGAC GCGCGTGAGG AAGAGTTCTT GCAGCTCGGT GACCCGCTCG
  841 ATGTGGCGGT CCGGATCGAC GGTGTGGCGC GTGGCGGGGT AGTCGGCGAA CGCGGCGGCG
  901 AGGGTGCGTA CGGCCCTGGG GGACGTCGTC GCGGGTGGCG AGGCGCACCG TGGGCTTGTA
  961 CTCGGTCATG GTTGTGGCCA TATTATCATC GTGTTTTTCA AAGGAAACCA CGTCCCCGTG
 1021 GGTTCGGGGG GGCTTAGACG TTTTTTTTAA CCTCGACTAA ACAACATGTA AAAGCATGTT
 1081 GCACCGAGGG CCCCAGATCA GATCCCCATA CCAATGGGGT ACCCTTCTTG GGCATCTTCA
 1141 GCCCCTGCTG AAATACGCTT GAAGGAAGAG CCATTGGACT CTTCCCACAC CTATTTCCAA
1201 ACTCAACAAC CGTTGG
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Please visit Sequencing and PCR primers for primer information.


References

References

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.
reference Hung, S.S., Repression of global protein synthesis by Eif1a-like genes that are expressed specifically in the two-cell embryos and the transient Zscan4-positive state of embryonic stem cells. DNA Res. 2013 Aug;20(4):391-402 (2013). PMID 23649898.
reference Hirata, T., Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells. Sci. Rep. 2: 208 (2012).. PMID 22355722.

2018.12.11

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