Reporter construct of Lefty1 minimal promoter.
Alternative name | pDelSacI-luc, pLefty-luc deltaSacI; stock no 2109 |
---|---|
Clone info. | Reporter construct with luciferase expression under the Lefty1 minimal promoter (-76 to +82 relative to the transcription start site of the Lefty1). |
Comment | Generated by digestion of pLefty1-luc with SacI, followed by T4 DNA polymerase treatment and self ligation. See Fig.1C of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006. |
Vector backbone | pGL3-basic (Plasmid) |
Gene/insert name | Mouse Lefty1 genomic DNA |
Depositor|Developer | Niwa, Hitoshi | |
Sequence | RDB07097z.seq |
---|
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006)) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 26, 7772-7782 (2006))。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
---|---|---|---|---|
RDB07097 | pLefty1-luc deltaSacI | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pLefty1-luc deltaSacI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07097). |
Reference section:
Nakatake, Y., Fukui, N., Iwamatsu, Y., Masui, S., Takahashi, K., Yagi, R., Yagi, K., Miyazaki, J., Matoba, R., Ko, M.S., Niwa, H., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP] |
---|
Featured content