Resource data sheet
Prev.

pLefty1-luc deltaSacI

Reporter construct of Lefty1 minimal promoter.

Catalog number RDB07097
Resource name pLefty1-luc deltaSacI
Alternative name pDelSacI-luc, pLefty-luc deltaSacI; stock no 2109
Clone info. Reporter construct with luciferase expression under the Lefty1 minimal promoter (-76 to +82 relative to the transcription start site of the Lefty1).
Generated by digestion of pLefty1-luc with SacI, followed by T4 DNA polymerase treatment and self ligation. See Fig.1C of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse Lefty1 genomic DNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank mouse Lefty1 (NCBI Gene 13590) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006))
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07097 pLefty1-luc deltaSacI DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07097z.seq from Depositor

Featured content

Featured content Reguration of genes in ES cells (English text)

References

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

2019.03.11

GNP_filter3_RDBDEP_html_190307.pl