Resource data sheet
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pLefty1-luc (#RDB07096)

Oct-Sox enhancer carrying reporter derived from Lefty1 promoter.

Alternative name stock no 972
Clone info. Reporter construct with luciferase expression under the Lefty1 promoter.
Comment Please use a new catalog number RDB10004 (pLefty1p-luc). Genomic fragment was amplified by PCR with primers 5'- CCTTTACACTGGTCTCGAGCC-3' and 5'-ATAAGCTTGTCCGGGAAGAGGAGCCTTG-3'. Resulting fragment contains 1,379 bp from -1297 to +82 relative to the transcription start site of the Lefty1. See Fig.1 of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse Lefty1 genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB07096z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006)).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006))。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB07096 pLefty1-luc Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pLefty1-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07096).

Reference section:

Nakatake, Y., Fukui, N., Iwamatsu, Y., Masui, S., Takahashi, K., Yagi, R., Yagi, K., Miyazaki, J., Matoba, R., Ko, M.S., Niwa, H., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]