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pLefty1-luc

Oct-Sox enhancer carrying reporter derived from Lefty1 promoter.

Catalog number RDB07096
Resource name pLefty1-luc
Alternative name stock no 972
Clone info. Reporter construct with luciferase expression under the Lefty1 promoter.
Please use a new catalog number RDB10004 (pLefty1p-luc). Genomic fragment was amplified by PCR with primers 5'- CCTTTACACTGGTCTCGAGCC-3' and 5'-ATAAGCTTGTCCGGGAAGAGGAGCCTTG-3'. Resulting fragment contains 1,379 bp from -1297 to +82 relative to the transcription start site of the Lefty1. See Fig.1 of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse Lefty1 genomic DNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank mouse Lefty1 (NCBI Gene 13590) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006))
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07096 pLefty1-luc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07096z.seq from Depositor

Featured content

Featured content Reguration of genes in ES cells (English text)

References

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

2019.03.11

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