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pmAID-ER-FBG

Retroviral vector of mouse Aicda-ER

Catalog number RDB07086
Resource name pmAID-ER-FBG
Alternative name #11470
Clone info. Retroviral vector of mouse Aicda-ER. Expression is under the control of MMLV promoter. Activity of Aicda is under the control of hormone binding domain of estrogen receptor ER-T2 mutant. Products are detectable by FLAG-tag. This vector also harbors GFP.
Vector backbone pFB-IRES-GFP (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Aid/Arp2 cDNA
Depositor|Developer Honjo, Tasuku |
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External Database
Mouse Aid/Arp2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U S A, 100, 2634-2638 (2003)). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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          MTA, for use for not-for-profit academic purpose [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U S A, 100, 2634-2638 (2003).)。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB07086 pmAID-ER-FBG Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
          JPY 18,920 (for-profit-research purpose)
          plus cost of shipping containers, dry ice (if required) and shipping charge

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pmAID-ER-FBG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07086).

          Reference section:

          Doi, T., Kinoshita, K., Ikegawa, M., Muramatsu, M., Honjo. T., De novo protein synthesis is required for the activation-induced cytidine deaminase function in class-switch recombination. Proc. Natl. Acad. Sci. U S A, 100, 2634-2638 (2003). PMID 12591955. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Doi, T., De novo protein synthesis is required for the activation-induced cytidine deaminase function in class-switch recombination. Proc. Natl. Acad. Sci. U S A, 100, 2634-2638 (2003). PMID 12591955. [link to RRC of NBRP]

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          2023.10.05

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