Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pdxEFFlag_EGFPit2 (#RDB07071)

Expression vector of EGFP.

Alternative name U2608.3d.c1
Clone info. Expression vector of EGFP.
Vector backbone pAxEFwtit2 (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。 2. 謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07071 pdxEFFlag_EGFPit2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pdxEFFlag_EGFPit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07071).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07071_A4Hep1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Sequence file: RDB07071_A4Hea.seq check
>07071_07071_A9DqF_2_EF1a-C_F_A01_01_ABI08.ab1
    1 TTATGGGGGA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTAATTCAT CGATTTATAG
  121 GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG CGGCCATGGT
  181 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  241 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  301 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  361 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  421 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  481 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  541 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  601 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  661 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  721 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  781 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  841 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
  901 CAGATCTCGA GCTCAAGCTT CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC
  961 CGGATCTAGA TAAGGCCGCT CGAGAAATTA TAAACTAGTC TAGAATCGAT GAACTTGTTT
 1021 ATTGCAGCTT ATAATGGGTT ACAAATAAAG CATAGCATCA CAATTTCACA AATAAAGCAT
 1081 TTTTTTCACT GCATTCTAGT GGTGGGTTTG TCAAACTCAT TCAATGTATC CTAATCATGT
 1141 CTGGAATTCT CGAGGCTAGC CTGCTTACGA TGCTGGACTA TGAGTGAGTG AAGCCCGTGC
1201 A
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles