Resource data sheet
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pdxEFFlag_EGFPit2

Expression vector of EGFP.

Catalog number RDB07071
Resource name pdxEFFlag_EGFPit2
Alternative name U2608.3d.c1
Clone info. Expression vector of EGFP.
Vector backbone pAxEFwtit2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Reference of nucleotide sequence U55763 (DDBJ accession number)
Depositor DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07071 pdxEFFlag_EGFPit2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07071_A4Hep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB07071_A4Hea.seq
>07071_07071_A9DqF_2_EF1a-C_F_A01_01_ABI08.ab1
    1 TTATGGGGGA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTAATTCAT CGATTTATAG
  121 GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG CGGCCATGGT
  181 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  241 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  301 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  361 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  421 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  481 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  541 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  601 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  661 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  721 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  781 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  841 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
  901 CAGATCTCGA GCTCAAGCTT CGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCAC
  961 CGGATCTAGA TAAGGCCGCT CGAGAAATTA TAAACTAGTC TAGAATCGAT GAACTTGTTT
 1021 ATTGCAGCTT ATAATGGGTT ACAAATAAAG CATAGCATCA CAATTTCACA AATAAAGCAT
 1081 TTTTTTCACT GCATTCTAGT GGTGGGTTTG TCAAACTCAT TCAATGTATC CTAATCATGT
 1141 CTGGAATTCT CGAGGCTAGC CTGCTTACGA TGCTGGACTA TGAGTGAGTG AAGCCCGTGC
1201 A
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.05.10

GNP_filter3_RDBDEP_html_190430.pl