Resource data sheet
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Vox (#RDB07040)

Plasmid clone to construct knock-in vectors

Clone info. Vox is a Gateway(R) entry clone containing Venus gene and neomycine-resistance gene casette driven by pgk promoter. Coding sequenceis flanked by attL1 and attL2 sites, for recombination.
Comment ES cells can be selected with G418. Expression of Venus was confirmed by the depositor. For sequencing, use the following primers as well as M13F and M13R: GFPR(500)_2, GTT GTG GCG GAT CTT GAA G; neoC1, TCG TGC TTT ACG GTA TCG CCG CTC CCG ATT; neoC2, GCC TGC TTG CCG AAT ATC ATG GTG GAA AAT.
Vector backbone pDONR221 (Plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Sasai, Yoshiki | Ikeya, Makoto |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Int. J. Dev. Biol., 49, 807-823 (2005)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は寄託者から事前に提供承諾書を取得すること。　利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Int. J. Dev. Biol., 49, 807-823 (2005))。非営利機関の非営利学術研究に限って提供する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB07040 Vox Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The Vox was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07040).

Reference section:

Ikeya, M., Kawada, M., Kiyonari, H., Sasai, N., Nakao, K., Furuta, Y., Sasai, Y., Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development, 133, 4463-4473 (2006). PMID 17035289. [link to RRC of NBRP]
Ikeya, M., Kawada, M., Nakazawa, Y., Sakuragi, M., Sasai, N., Ueno, M., Kiyonari, H., Nakao, K., Sasai, Y., Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol., 49, 807-823 (2005). PMID 16172977. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ikeya, M., Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development, 133, 4463-4473 (2006). PMID 17035289. [link to RRC of NBRP]
original Ikeya, M., Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol., 49, 807-823 (2005). PMID 16172977. [link to RRC of NBRP]