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nBLUEneo (#RDB07039)

Plasmid clone to construct knock-in vectors

Clone info. nBLUEneo is a Gateway(R) entry clone containing beta-galactosidase gene with nuclear localization signal, and neomycine-resistance gene casette driven by pgk promoter. Coding sequenceis flanked by attL1 and attL2 sites, for recombination.
Comment ES cells can be selected with G418. Expression of beta-galactosidase was confirmed by the depositor. For sequencing, use the following primers as well as M13F and M13R: LacZ(68->49), TTA AGT TGG GTA ACG CCA GG; neoC1, TCG TGC TTT ACG GTA TCG CCG CTC CCG ATT; neoC2, GCC TGC TTG CCG AAT ATC ATG GTG GAA AAT.
Vector backbone pDONR221 (Plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Depositor|Developer Sasai, Yoshiki | Ikeya, Makoto |
 

Distribution information

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Terms and conditions for distribution This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Int. J. Dev. Biol., 49, 807-823 (2005)).
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提供案内 (日本国内) [open/close]

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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB07039 nBLUEneo Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The nBLUEneo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07039).

Reference section:

Ikeya, M., Kawada, M., Kiyonari, H., Sasai, N., Nakao, K., Furuta, Y., Sasai, Y., Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development, 133, 4463-4473 (2006). PMID 17035289. [link to RRC of NBRP]
Ikeya, M., Kawada, M., Nakazawa, Y., Sakuragi, M., Sasai, N., Ueno, M., Kiyonari, H., Nakao, K., Sasai, Y., Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol., 49, 807-823 (2005). PMID 16172977. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ikeya, M., Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development, 133, 4463-4473 (2006). PMID 17035289. [link to RRC of NBRP]
original Ikeya, M., Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol., 49, 807-823 (2005). PMID 16172977. [link to RRC of NBRP]