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p6W5'tk-luc

Reporter construct with luciferase expression under 6W enhancer, activated by Oct3/4.

Catalog number RDB07033
Resource name p6W5'tk-luc
Alternative name stock no 667; ptk-luc 6W5'
Clone info. Reporter construct with luciferase expression under 6W enhancer (immunoglobulin heavy chain), which is activated by Oct3/4 alone.
6W enhancer containing 6 copies of oligonucleotides with octamer-binding motif from the mouse immunoglobulin heavy chai gene enhancer (Niwa, H., Mol. Cell. Biol., 1526-1536, 2002). Use ptk-luc (RDB07031) as a control.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse immunoglobulin heavy chain cDNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank mouse Ighm (NCBI Gene 16019) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Mol. Cell. Biol., 22, 1526-1536 (2002).
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07033 p6W5'tk-luc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07033z.seq from Depositor
Electronic file Map RDB07033a.gif from Depositor

Featured content

Featured content Reguration of genes in ES cells (English text)

References

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

2018.12.10

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