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pFGF4tk-luc

Reporter carrying Oct-Sox enhancer derived from Fgf4 gene.

Catalog number RDB07032
Resource name pFGF4tk-luc
Alternative name stock no 666; ptk-luc Fgf4enh5'
Clone info. Reporter construct with luciferase expression under the mouse FGF4 and tk promoter.
Comment 460-bp PCR-amplified genomic DNA carrying the Fgf-4 enhancer placed upstream of the tk-luc cassette in plasmid ptk-luc (Mol. Cell. Biol. 22 (5) 1526-1536, 2002). Use ptk-luc (RDB07031; stock no. 649) as a control.
Vector backbone ptk-luc (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Fgf4 genomic DNA
Depositor|Developer Niwa, Hitoshi |
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External Database
Mouse Fgf4

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Niwa, H., Mol. Cell. Biol., 22, 1526-1536 (2002)).
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Niwa, H., Mol. Cell. Biol., 22, 1526-1536 (2002))
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供依頼書 [Word]
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB07032 pFGF4tk-luc Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence RDB07032z.seq
          Remarks, protocol and/or map (gif) RDB07032a.gif

          Original reference

          original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Control Vectors (English text)
          Featured content Reguration of genes in ES cells (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

          2022.05.17

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