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pFGF4tk-luc

Reporter carrying Oct-Sox enhancer derived from Fgf4 gene.

Catalog number RDB07032
Resource name pFGF4tk-luc
Alternative name stock no 666; ptk-luc Fgf4enh5'
Clone info. Reporter construct with luciferase expression under the mouse FGF4 and tk promoter.
460-bp PCR-amplified genomic DNA carrying the Fgf-4 enhancer placed upstream of the tk-luc cassette in plasmid ptk-luc (Mol. Cell. Biol. 22 (5) 1526-1536, 2002). Use ptk-luc (RDB07031; stock no. 649) as a control.
Vector backbone ptk-luc (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Fgf4 genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank mouse Fgf4 (NCBI Gene 14175) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Niwa, H., Mol. Cell. Biol., 22, 1526-1536 (2002)).
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB07032 pFGF4tk-luc Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07032z.seq
Electronic file Map RDB07032a.gif

Original reference

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Control Vectors (English text)
Featured content Reguration of genes in ES cells (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Niwa, H., Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol., 22, 1526-1536 (2002). PMID 11839818.

2021.03.25

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